Research on the equipment, process, and commercialization progress of syngas fermentation

BAI Xue; LIANG Nan; LI Xinqi; MO Zhipeng; TONG Shuhuan; YUE Meiqi; JIA Xiaojing; REN Kang; XI Xiaojie; CHAO Wei

doi:10.12211/2096-8280.2025-001

您当前的位置：

首页 >

文章列表页 >

Research on the equipment, process, and commercialization progress of syngas fermentation

更新时间：2025-02-24

- Research on the equipment, process, and commercialization progress of syngas fermentation
- Synthetic Biology Journal Pages: 1-22(2025)
- 作者机构：
  
  1.北京首钢朗泽科技股份有限公司，北京 100043
  2.河北首朗新能源科技有限公司，河北唐山 063200
- 作者简介：
- 基金信息：
- DOI：10.12211/2096-8280.2025-001
  CLC： Q815
- Received：02 January 2025，
  
  Revised：2025-02-21，
  
  Online First：24 February 2025，
- 稿件说明：
移动端阅览
白雪，梁楠，李欣启，莫志朋，佟淑环，岳美琪，贾晓静，任康，息晓杰，晁伟. 合成气发酵装备、工艺及商业化进展研究［J］. 合成生物学， 2025， 6. DOI： 10.12211/2096-8280.2025-001

BAI Xue， LIANG Nan， LI Xinqi， MO Zhipeng， TONG Shuhuan， YUE Meiqi， JIA Xiaojing， REN Kang， XI Xiaojie， CHAO Wei. Research on the equipment， process， and commercialization progress of syngas fermentation［J］. Synthetic Biology Journal， 2025， 6. DOI： 10.12211/2096-8280.2025-001
白雪，梁楠，李欣启，莫志朋，佟淑环，岳美琪，贾晓静，任康，息晓杰，晁伟. 合成气发酵装备、工艺及商业化进展研究［J］. 合成生物学， 2025， 6. DOI： 10.12211/2096-8280.2025-001 DOI：

BAI Xue， LIANG Nan， LI Xinqi， MO Zhipeng， TONG Shuhuan， YUE Meiqi， JIA Xiaojing， REN Kang， XI Xiaojie， CHAO Wei. Research on the equipment， process， and commercialization progress of syngas fermentation［J］. Synthetic Biology Journal， 2025， 6. DOI： 10.12211/2096-8280.2025-001 DOI：

摘要

在全球气候变暖日益加剧的背景下，探索并实施有效的碳减排策略，已成为应对这一全球性挑战的当务之急。合成气发酵技术作为一种减少温室气体排放、回收工业尾气并生产可再生燃料和化学品的绿色低碳技术，在全球气候缓解和循环经济转型中发挥着重要作用。工业菌种被认为是生物发酵的“芯片”，而在合成气发酵技术转化落地过程中，新装备、新工艺的开发同样重要。本文综述了在合成气发酵领域菌种端和工业应用端重要装备的研究进展，总结了包括原料气净化处理、气体发酵、产物分离提取等工艺在内的全流程合成气发酵集成技术的发展和面临的挑战，系统梳理了合成气发酵商业化项目的发展脉络，指出技术的工程转化与推广不仅要求技术上的革新突破，更需要前瞻的商业布局以及政策的支持，为合成气发酵技术的发展和商业化项目复制推广提供重要参考。

Abstract

In the context of the increasingly severe global climate warming

exploring and implementing effective carbon reduction strategies has become an urgent priority to address this global challenge. Syngas fermentation

as a green low-carbon technology that reduces greenhouse gas emissions

recycles industrial waste gas

and produces renewable fuels and chemicals

plays an important role in global climate mitigation and the transition to a circular economy. Industrial strains are considered the "chips" of fermentation

and in the process of transforming and implementing syngas fermentation technology

the development of new equipment and new processes is equally important. Firstly

this article reviews the research progress on important equipment in the fields of strain development and industrial application of syngas fermentation. It summarizes the latest advancements in high-throughput cultivation and screening of anaerobic microorganisms in syngas atmosphere. The application of this technology will accelerate the optimization and iteration of industrial strains for syngas fermentation

providing better-performing industrial strains for commercial application. Then

the review outlines the research progress of various types of reactors currently used for syngas fermentation

including stirred tank reactors

bubble column reactors

gas-lift reactors

trickle bed reactors

and hollow fiber membrane reactors

and discusses the potential and challenges of each type of reactor in industrial production. Among these reactors

the gas-lift reactor

due to its low energy consumption

simple structure

and good economic benefits

has been widely used in the commercial application of syngas fermentation. Secondly

the review summarizes the development and challenges faced by the integrated syngas fermentation technology

including raw gas purification

gas fermentation

and product separation and extraction processes. After an in-depth discussion of the development trajectory of commercialization projects for syngas fermentation

the review points out that the promotion of syngas fermentation not only require technological breakthroughs but also meticulous engineering management

forward-looking commercial planning

and strong policy support

all of which are core to advancing the technology from the laboratory to the market.

关键词

Keywords

references

CHU H , HUANG Z , ZHANG Z , et al . Integration of carbon emission reduction policies and technologies: Research progress on carbon capture, utilization and storage technologies [J ] . Separation and Purification Technology , 2024 , 343 : 127153 .

DAVOODI S , AL-SHARGABI M , WOOD D A , et al . Review of technological progress in carbon dioxide capture, storage, and utilization [J ] . Gas Science and Engineering , 2023 , 117 : 205070 .

JATAIN I , DUBEY K K , SHARMA M , et al . Synthetic biology potential for carbon sequestration into biocommodities [J ] . Journal of Cleaner Production , 2021 , 323 : 129176 .

LIU X , LUO H , YU D , et al . Synthetic biology promotes the capture of CO 2 to produce fatty acid derivatives in microbial cell factories [J ] . Bioresources and Bioprocessing , 2022 , 9 ( 1 ): 124 .

DELISI C , PATRINOS A , MACCRACKEN M , et al . The role of synthetic biology in atmospheric greenhouse gas reduction: prospects and challenges [J ] . Biodesign Research , 2020 , 2020 : 1016207 .

FACKLER N , HEIJSTRA B D , RASOR B J , et al . Stepping on the gas to a circular economy: Accelerating development of carbon-negative chemical production from gas fermentation [J ] . Annual Review of Chemical and Biomolecular Engineering , 2021 , 12 ( 1 ): 1 - 32 .

CALVO D C , LUNA H J , ARANGO J A , et al . Determining global trends in syngas fermentation research through a bibliometric analysis [J ] . Journal of Environmental Management , 2022 , 307 : 114522 .

RAGSDALE S W . Enzymology of the Wood–Ljungdahl pathway of acetogenesis [J ] . Annals of the New York Academy of Sciences , 2008 , 1125 ( 1 ): 129 - 136 .

LIEW F , HENSTRA A M , KӦPKE M , et al . Metabolic engineering of Clostridium autoethanogenum for selective alcohol production [J ] . Metabolic Engineering , 2017 , 40 : 104 - 114 .

LAUER I , PHILIPPS G , JENNEWEIN S . Metabolic engineering of Clostridium ljungdahlii for the production of hexanol and butanol from CO 2 and H 2 [J ] . Microbial Cell Factories , 2022 , 21 ( 1 ): 85 .

ARSLAN K , SCHOCH T , HÖFELE F , et al . Engineering Acetobacterium woodii for the production of isopropanol and acetone from carbon dioxide and hydrogen [J ] . Biotechnology Journal , 2022 , 17 ( 5 ): e2100515 .

MOON J , POEHLEIN A , DANIEL R , et al . Redirecting electron flow in Acetobacterium woodii enables growth on CO and improves growth on formate [J ] . Nature Communications , 2024 , 15 ( 1 ): 5424 .

JIA D , DENG W , HU P , et al . Thermophilic Moorella thermoacetica as a platform microorganism for C1 gas utilization: Physiology, engineering, and applications [J ] . Bioresources and Bioprocessing , 2023 , 10 ( 1 ): 61 .

SUN X , ATIYEH H K , HUHNKE R L , et al . Syngas fermentation process development for production of biofuels and chemicals: A review [J ] . Bioresource Technology Reports , 2019 , 7 : 100279 .

BENEVENUTI C , AMARAL P , FERREIRA T , et al . Impacts of syngas composition on anaerobic fermentation [J ] . Reactions , 2021 , 2 ( 4 ): 391 - 407 .

MUNASINGHE P C , KHANAL S K . Biomass-derived syngas fermentation into biofuels: Opportunities and challenges [J ] . Bioresource Technology , 2010 , 101 ( 13 ): 5013 - 5022 .

OWOADE A , ALSHAMI A S , LEVIN D , et al . Progress and development of syngas fermentation processes toward commercial bioethanol production [J ] . Biofuels, Bioproducts and Biorefining , 2023 , 17 ( 5 ): 1328 - 1342 .

BALEEIRO F C F , KLEINSTEUBER S , NEUMANN A , et al . Syngas-aided anaerobic fermentation for medium-chain carboxylate and alcohol production: The case for microbial communities [J ] . Applied Microbiology and Biotechnology , 2019 , 103 ( 21-22 ): 8689 - 8709 .

MOLITOR B , MARCELLIN E , ANGENENT L T . Overcoming the energetic limitations of syngas fermentation [J ] . Current Opinion in Chemical Biology , 2017 , 41 : 84 - 92 .

BENGELSDORF F R , STRAUB M , DÜRRE P . Bacterial synthesis gas (syngas) fermentation [J ] . Environmental Technology , 2013 , 34 ( 13-16 ): 1639 - 1651 .

NETO A S , WAINAINA S , CHANDOLIAS K , et al . Exploring the potential of syngas fermentation for recovery of high-value resources: A comprehensive review [J ] . Current Pollution Reports , 2024 , 11 ( 1 ): 7 .

WAINAINA S , HORVÁTH I S , TAHERZADEH M J . Biochemicals from food waste and recalcitrant biomass via syngas fermentation: A review [J ] . Bioresource Technology , 2018 , 248 ( Pt A ): 113 - 121 .

KIM J Y , LEE M , OH S , et al . Acetogen and acetogenesis for biological syngas valorization [J ] . Bioresource Technology , 2023 , 384 : 129368 .

LEE J . Lessons from clostridial genetics: Toward engineering acetogenic bacteria [J ] . Biotechnology and Bioprocess Engineering , 2021 , 26 ( 6 ): 841 - 858 .

ENRIQUEZ F A , AHRING B K . Strategies to overcome mass transfer limitations of hydrogen during anaerobic gaseous fermentations: A comprehensive review [J ] . Bioresource Technology , 2023 , 377 : 128948 .

PUIMAN L , ELISIÁRIO M P , CRASBORN L M L , et al . Gas mass transfer in syngas fermentation broths is enhanced by ethanol [J ] . Biochemical Engineering Journal , 2022 , 185 : 108505 .

YOU M , ZHAO Q , LIU Y , et al . Insights into lignocellulose degradation: Comparative genomics of anaerobic and cellulolytic ruminiclostridium-type species [J ] . Frontiers in Microbiology , 2023 , 14 : 1288286 .

STARK C , MÜNSSINGER S , ROSENAU F , et al . The potential of sequential fermentations in converting C1 substrates to higher-value products [J ] . Frontiers in Microbiology , 2022 , 13 : 907577 .

WHITE H , HUBER C , FEICHT R , et al . On a reversible molybdenum-containing aldehyde oxidoreductase from Clostridium formicoaceticum [J ] . Archives of Microbiology , 1993 , 159 ( 3 ): 244 - 249 .

FAST A G , PAPOUTSAKIS E T . Stoichiometric and energetic analyses of non-photosynthetic CO 2 -fixation pathways to support synthetic biology strategies for production of fuels and chemicals [J ] . Current Opinion in Chemical Engineering , 2012 , 1 ( 4 ): 380 - 395 .

YAMAMOTO I , SAIKI T , LIU S M , et al . Purification and properties of NADP-dependent formate dehydrogenase from Clostridium thermoaceticum , a tungsten-selenium-iron protein [J ] . Journal of Biological Chemistry , 1983 , 258 ( 3 ): 1826 - 1832 .

MOON M , PARK G W , LEE J P , et al . Recombinant expression and characterization of formate dehydrogenase from Clostridium ljungdahlii (ClFDH) as CO 2 reductase for converting CO 2 to formate [J ] . Journal of CO 2 Utilization , 2022 , 57 : 101876 .

NAGARAJU S , DAVIES N K , WALKER D J F , et al . Genome editing of Clostridium autoethanogenum using CRISPR/Cas9 [J ] . Biotechnology for Biofuels , 2016 , 9 ( 1 ): 219 .

FACKLER N , HEFFERNAN J , JUMINAGA A , et al . Transcriptional control of Clostridium autoethanogenum using CRISPRi [J ] . Synthetic Biology , 2021 , 6 ( 1 ): ysab008 .

POULALIER-DELAVELLE M , BAKER J P , MILLARD J , et al . Endogenous CRISPR/Cas systems for genome engineering in the acetogens Acetobacterium woodii and Clostridium autoethanogenum [J ] . Frontiers in Bioengineering and Biotechnology , 2023 , 11 : 1213236 .

CHARUBIN K , STREETT H , PAPOUTSAKIS E T . Development of strong anaerobic fluorescent reporters for Clostridium acetobutylicum and Clostridium ljungdahlii using HaloTag and SNAP-tag proteins [J ] . Applied and Environmental Microbiology , 2020 , 86 ( 20 ): e01271-20 .

STREETT H E , KALIS K M , PAPOUTSAKIS E T . A Strongly fluorescing anaerobic reporter and protein-tagging system for Clostridium organisms based on the fluorescence-activating and absorption-shifting tag protein (FAST) [J ] . Applied and Environmental Microbiology , 2019 , 85 ( 14 ): e00622-19 .

KWON K K , LEE J , KIM H , et al . Advancing high-throughput screening systems for synthetic biology and biofoundry [J ] . Current Opinion in Systems Biology , 2024 , 37 : 100487 .

ZENG W , GUO L , XU S , et al . High-throughput screening technology in industrial biotechnology [J ] . Trends in Biotechnology , 2020 , 38 ( 8 ): 888 - 906 .

WANG Y , LI S , XUE N , et al . Modulating sensitivity of an erythromycin biosensor for precise high-throughput screening of strains with different characteristics [J ] . ACS Synthetic Biology , 2023 , 12 ( 6 ): 1761 - 1771 .

WONG B G , MANCUSO C P , KIRIAKOV S , et al . Precise, automated control of conditions for high-throughput growth of yeast and bacteria with eVOLVER [J ] . Nature Biotechnology , 2018 , 36 ( 7 ): 614 - 623 .

SUNDSTROM E R , CRIDDLE C S . Optimization of methanotrophic growth and production of poly(3-hydroxybutyrate) in a high-throughput microbioreactor system [J ] . Applied and Environmental Microbiology , 2015 , 81 ( 14 ): 4767 - 4773 .

KÖPKE M , SIMPSON S D . Pollution to products: recycling of 'above ground' carbon by gas fermentation [J ] . Current Opinion in Biotechnology , 2020 , 65 : 180 - 189 .

TU R , ZHANG Y , HUA E , et al . Droplet-based microfluidic platform for high-throughput screening of Streptomyces [J ] . Communications Biology , 2021 , 4 ( 1 ): 647 .

CHAO R , MISHRA S , SI T , et al . Engineering biological systems using automated biofoundries [J ] . Metabolic Engineering , 2017 , 42 : 98 - 108 .

MUNASINGHE P C , KHANAL S K . Syngas fermentation to biofuel: Evaluation of carbon monoxide mass transfer coefficient ( K L a ) in different reactor configurations [J ] . Biotechnology Progress , 2010 , 26 ( 6 ): 1616 - 1621 .

UNGERMAN A J , HEINDEL T J . Carbon monoxide mass transfer for syngas fermentation in a stirred tank reactor with dual impeller configurations [J ] . Biotechnology Progress , 2007 , 23 ( 3 ): 613 - 620 .

ASIMAKOPOULOS K , GAVALA H N , SKIADAS I V . Reactor systems for syngas fermentation processes: A review [J ] . Chemical Engineering Journal , 2018 , 348 : 732 - 744 .

RIGGS S S , HEINDEL T J . Measuring carbon monoxide gas—liquid mass transfer in a stirred tank reactor for syngas fermentation [J ] . Biotechnology Progress , 2006 , 22 ( 3 ): 903 - 906 .

BREDWELL , SRIVASTAVA , WORDEN . Reactor design issues for synthesis-gas fermentations [J ] . Biotechnology Progress , 1999 , 15 ( 5 ): 834 - 844 .

ABUBACKAR H N , VEIGA M C , KENNES C . Biological conversion of carbon monoxide: Rich syngas or waste gases to bioethanol [J ] . Biofuels, Bioproducts and Biorefining , 2011 , 5 ( 1 ): 93 - 114 .

KANTARCI N , BORAK F , ULGEN K O . Bubble column reactors [J ] . Process Biochemistry , 2005 , 40 ( 7 ): 2263 - 2283 .

RAJAGOPALAN S , DATAR R P , LEWIS R S . Formation of ethanol from carbon monoxide via a new microbial catalyst [J ] . Biomass and Bioenergy , 2002 , 23 ( 6 ): 487 - 493 .

XU X , ZHANG Y . Hydrodynamics and mass transfer in an airlift loop reactor: Comparison between using two kinds of spargers [J ] . Processes , 2023 , 12 ( 1 ): 35 .

KOMMAREDDY A , ANDERSON G . Analysis of currents and mixing in a modified bubble column reactor [J ] . 2004 , Ottawa , Canada August 1 - 4 , 2004 , 2004 .

FADAVI A , CHISTI Y . Gas–liquid mass transfer in a novel forced circulation loop reactor [J ] . Chemical Engineering Journal , 2005 , 112 ( 1-3 ): 73 - 80 .

RÜCKEL A , HANNEMANN J , MAIERHOFER C , et al . Studies on syngas fermentation with Clostridium carboxidivorans in stirred-tank reactors with defined gas impurities [J ] . Frontiers in Microbiology , 2021 , 12 : 655390 .

RÜCKEL A , OPPELT A , LEUTER P , et al . Conversion of syngas from entrained flow gasification of biogenic residues with Clostridium carboxidivorans and Clostridium autoethanogenum [J ] . Fermentation , 2022 , 8 ( 9 ): 465 .

RIEGLER P , CHRUSCIEL T , MAYER A , et al . Reversible retrofitting of a stirred-tank bioreactor for gas-lift operation to perform synthesis gas fermentation studies [J ] . Biochemical Engineering Journal , 2019 , 141 : 89 - 101 .

ZHANG L , HU P , PAN J , et al . Immobilization of trophic anaerobic acetogen for semi-continuous syngas fermentation [J ] . Chinese Journal of Chemical Engineering , 2021 , 29 : 311 - 316 .

SERTKAYA S , GUNDOGDU T K , KENNES C , et al . Bioethanol production through syngas fermentation by a novel immobilized bioreactor using Clostridium ragsdalei [J ] . Icontech International Journal , 2021 , 5 ( 3 ): 13 - 20 .

YASIN M , PARK S , JEONG Y , et al . Effect of internal pressure and gas/liquid interface area on the CO mass transfer coefficient using hollow fibre membranes as a high mass transfer gas diffusing system for microbial syngas fermentation [J ] . Bioresource Technology , 2014 , 169 : 637 - 643 .

SHEN Y , BROWN R , WEN Z . Syngas fermentation of Clostridium carboxidivoran P7 in a hollow fiber membrane biofilm reactor: Evaluating the mass transfer coefficient and ethanol production performance [J ] . Biochemical Engineering Journal , 2014 , 85 : 21 - 29 .

DEVARAPALLI M , LEWIS R S , ATIYEH H K . Continuous ethanol production from synthesis gas by Clostridium ragsdalei in a trickle-bed reactor [J ] . Fermentation , 2017 , 3 ( 2 ): 23 .

SHEN Y , BROWN R , WEN Z . Enhancing mass transfer and ethanol production in syngas f ermentation of Clostridium carboxidivorans P7 through a monolithic biofilm reactor [J ] . Applied Energy , 2014 , 136 : 68 - 76 .

SHEN Y , BROWN R C , WEN Z . Syngas fermentation by Clostridium carboxidivorans P7 in a horizontal rotating packed bed biofilm reactor with enhanced ethanol production [J ] . Applied Energy , 2017 , 187 : 585 - 594 .

STOLL I K , BOUKIS N , SAUER J . Syngas fermentation to alcohols: Reactor technology and application perspective [J ] . Chemie Ingenieur Technik , 2020 , 92 ( 1-2 ): 125 - 136 .

CHEN J , GOMEZ J A , HÖFFNER K , et al . Metabolic modeling of synthesis gas fermentation in bubble column reactors [J ] . Biotechnology for Biofuels , 2015 , 8 ( 1 ): 89 .

BENALCÁZAR E A , NOORMAN H , FILHO R M , et al . Modeling ethanol production through gas fermentation: A biothermodynamics and mass transfer-based hybrid model for microbial growth in a large-scale bubble column bioreactor [J ] . Biotechnology for Biofuels , 2020 , 13 ( 1 ): 59 .

LEE M , YASIN M , JANG N , et al . A simultaneous gas feeding and cell-recycled reaction (SGCR) system to achieve biomass boosting and high acetate titer in microbial carbon monoxide fermentation [J ] . Bioresource Technology , 2020 , 298 : 122549 .

LEE P H , NI S Q , CHANG S Y , et al . Enhancement of carbon monoxide mass transfer using an innovative external hollow fiber membrane (HFM) diffuser for syngas fermentation: Experimental studies and model development [J ] . Chemical Engineering Journal , 2012 , 184 : 268 - 277 .

LU C , CHENG W , LI Z , et al . Influence of Aeration rates on oxygen mass transfer and flow- field in a microporous aeration system [J ] . Polish Journal of Environmental Studies , 2021 , 30 ( 4 ): 3727 - 3739 .

AHMED T , SEMMENS M J . Use of sealed end hollow fibers for bubbleless membrane aeration: experimental studies [J ] . Journal of Membrane Science , 1992 , 69 ( 1-2 ): 1 - 10 .

JENSEN M B , OTTOSEN L D M , KOFOED M V W . H 2 gas-liquid mass transfer: A key element in biological Power-to-Gas methanation [J ] . Renewable and Sustainable Energy Reviews , 2021 , 147 : 111209 .

EBRAHIMI S , KLEEREBEZEM R , KREUTZER M T , et al . Potential application of monolith packed columns as bioreactors, control of biofilm formation [J ] . Biotechnology and Bioengineering , 2006 , 93 ( 2 ): 238 - 245 .

EBRAHIMI S , PICIOREANU C , XAVIER J B , et al . Biofilm growth pattern in honeycomb monolith packings: Effect of shear rate and substrate transport limitations [J ] . Catalysis Today , 2005 , 105 ( 3-4 ): 448 - 454 .

BEYGMOHAMMDI F , KAZEROUNI H N , JAFARZADEH Y , et al . Preparation and characterization of PVDF/PVP-GO membranes to be used in MBR system [J ] . Chemical Engineering Research and Design , 2020 , 154 : 232 - 240 .

DJAMEL G . New configurations and techniques for controlling membrane bioreactor (MBR) fouling [J ] . Open Access Library Journal , 2020 , 07 ( 07 ): 1 - 18 .

MEDEIROS E M de , NOORMAN H , FILHO R M , et al . Production of ethanol fuel via syngas fermentation: Optimization of economic performance and energy efficiency [J ] . Chemical Engineering Science: X , 2020 , 5 : 100056 .

TAKORS R , KOPF M , MAMPEL J , et al . Using gas mixtures of CO, CO 2 and H 2 as microbial substrates: The do's and don'ts of successful technology transfer from laboratory to production scale [J ] . Microbial Biotechnology , 2018 , 11 ( 4 ): 606 - 625 .

ELISIÁRIO M P , WEVER H D , HECKE W V , et al . Membrane bioreactors for syngas permeation and fermentation [J ] . Critical Reviews in Biotechnology , 2022 , 42 ( 6 ): 856 - 872 .

KURT E , QIN J , WILLIAMS A , et al . Perspectives for using CO 2 as a feedstock for biomanufacturing of fuels and chemicals [J ] . Bioengineering , 2023 , 10 ( 12 ): 1357 .

OSWALD F , ZWICK M , OMAR O , et al . Growth and product formation of Clostridium ljungdahlii in presence of cyanide [J ] . Frontiers in Microbiology , 2018 , 9 : 1213 .

FRILUND C , KOTILAINEN M , LORENZO J B , et al . Steel manufacturing EAF dust as a potential adsorbent for hydrogen sulfide removal [J ] . Energy & Fuels , 2022 , 36 ( 7 ): 3695 - 3703 .

CILIBERTI C , BIUNDO A , ALBERGO R , et al . Syngas derived from lignocellulosic biomass gasification as an alternative resource for innovative bioprocesses [J ] . Processes , 2020 , 8 ( 12 ): 1567 .

LÓPEZ P A , REBECCHI S , VLAEMINCK E , et al . Demonstrating pilot-scale gas fermentation for acetate production from biomass-derived syngas streams [J ] . Fermentation , 2024 , 10 ( 6 ): 285 .

HARAHAP B M , AHRING B K . Acetate production from syngas produced from lignocellulosic biomass materials along with gaseous fermentation of the syngas: A review [J ] . Microorganisms , 2023 , 11 ( 4 ): 995 .

SAXENA J , TANNER R S . Optimization of a corn steep medium for production of ethanol from synthesis gas fermentation by Clostridium ragsdalei [J ] . World Journal of Microbiology and Biotechnology , 2012 , 28 ( 4 ): 1553 - 1561 .

ABUBACKAR H N , VEIGA M C , KENNES C . Carbon monoxide fermentation to ethanol by Clostridium autoethanogenum in a bioreactor with no accumulation of acetic acid [J ] . Bioresource Technology , 2015 , 186 : 122 - 127 .

LI D , MENG C , WU G , et al . Effects of zinc on the production of alcohol by Clostridium carboxidivorans P7 using model syngas [J ] . Journal of Industrial Microbiology & Biotechnology , 2018 , 45 ( 1 ): 61 - 69 .

SHEN S , WANG G , ZHANG M , et al . Effect of temperature and surfactant on biomass growth and higher-alcohol production during syngas fermentation by Clostridium carboxidivorans P7 [J ] . Bioresources and Bioprocessing , 2020 , 7 ( 1 ): 56 .

LIU C , LUO G , WANG W , et al . The effects of pH and temperature on the acetate production and microbial community compositions by syngas fermentation [J ] . Fuel , 2018 , 224 : 537 - 544 .

HURST K M , LEWIS R S . Carbon monoxide partial pressure effects on the metabolic process of syngas fermentation [J ] . Biochemical Engineering Journal , 2010 , 48 ( 2 ): 159 - 165 .

OSWALD F , STOLL I K , ZWICK M , et al . Formic acid formation by Clostridium ljungdahlii at elevated pressures of carbon dioxide and hydrogen [J ] . Frontiers in Bioengineering and Biotechnology , 2018 , 6 : 6 .

MAKSIMOV P , LAARI A , RUUSKANEN V , et al . Gas phase methanol synthesis with Raman spectroscopy for gas composition monitoring [J ] . RSC Advances , 2020 , 10 ( 40 ): 23690 - 23701 .

METCALFE G D , SMITH T W , HIPPLER M . On-line analysis and in situ pH monitoring of mixed acid fermentation by Escherichia coli using combined FTIR and Raman techniques [J ] . Analytical and Bioanalytical Chemistry , 2020 , 412 ( 26 ): 7307 - 7319 .

DANG J , WANG N , ATIYEH H K . Review of dissolved CO and H 2 measurement methods for syngas fermentation [J ] . Sensors , 2021 , 21 ( 6 ): 2165 .

PUIMAN L , BENALCÁZAR E A , PICIOREANU C , et al . Downscaling industrial-scale syngas fermentation to simulate frequent and irregular dissolved gas concentration shocks [J ] . Bioengineering , 2023 , 10 ( 5 ): 518 .

RICHTER H , MARTIN M , ANGENENT L . A two-stage continuous fermentation system for conversion of syngas into ethanol [J ] . Energies , 2013 , 6 ( 8 ): 3987 - 4000 .

ROBLES-IGLESIAS R , NAVEIRA-PAZOS C , NICAUD J M , et al . Two-stage syngas fermentation into microbial oils and β-carotene with Clostridium carboxidivorans and engineered Yarrowia lipolytica [J ] . Journal of CO 2 Utilization , 2023 , 76 : 102593 .

SIVALINGAM V , AHMADI V , BABAFEMI O , et al . Integrating syngas fermentation into a single-cell microbial electrosynthesis (MES) reactor [J ] . Catalysts , 2020 , 11 ( 1 ): 40 .

PU Y , WANG Y , WU G , et al . Tandem acidic CO 2 electrolysis coupled with syngas fermentation: a two-stage process for producing medium-chain fatty acids [J ] . Environmental Science & Technology , 2024 , 58 ( 17 ): 7445 - 7456 .

VERBEECK K , GILDEMYN S , RABAEY K . Membrane electrolysis assisted gas fermentation for enhanced acetic acid production [J ] . Frontiers in Energy Research , 2018 , 6 : 88 .

KUTTASSERY F , KUMAGAI H , KAMATA R , et al . Supramolecular photocatalysts fixed on the inside of the polypyrrole layer in dye sensitized molecular photocathodes: application to photocatalytic CO 2 reduction coupled with water oxidation [J ] . Chemical Science , 2021 , 12 ( 39 ): 13216 - 13232 .

VERMA P , STEWART D J , RAJA R . Recent advances in photocatalytic CO 2 utilisation over multifunctio nal metal–organic frameworks [J ] . Catalysts , 2020 , 10 ( 10 ): 1176 .

JANKOVIĆ T , STRAATHOF A J J , MCGREGOR I R , et al . Bioethanol separation by a new pass-through distillation process [J ] . Separation and Purification Technology , 2024 , 336 : 126292 .

ZENTOU H , ABIDIN Z Z , YUNUS R , et al . Overview of alternative ethanol removal techniques for enhancing bioethanol recovery from fermentation broth [J ] . Processes , 2019 , 7 ( 7 ): 458 .

DEVI N B , PUGAZHENTHI G , PAKSHIRAJAN K . Synthetic biology approaches and bioseparations in syngas fermentation [J ] . Trends in Biotechnology , 2025 , 43 ( 1 ): 111 - 130 .

HUANG T Y , HO J S , GOH S , et al . Ethanol recovery from dilute aqueous solution by perstraction using supported ionic liquid membrane (SILM) [J ] . Journal of Cleaner Production , 2021 , 298 : 126811 .

DIMITRIJEVIĆ D , BÖSENHOFER M , HARASEK M . Liquid–liquid phase separation of two non-dissolving liquids—A mini review [J ] . Processes , 2023 , 11 ( 4 ): 1145 .

REDL S , SUKUMARA S , PLOEGER T , et al . Thermodynamics and economic feasibility of acetone production from syngas usin g the thermophilic production host Moorella thermoacetica [J ] . Biotechnology for Biofuels , 2017 , 10 ( 1 ): 150 .

JEAN A B , BROWN R C . Techno-economic analysis of gas fermentation for the production of single cell protein [J ] . Environmental Science & Technology , 2024 , 58 ( 8 ): 3823 - 3829 .

VLAEMINCK E , UITTERHAEGEN E , QUATAERT K , et al . Single-cell protein production from industrial off-gas through acetate: Techno-economic analysis for a coupled fermentation approach [J ] . Fermentation , 2023 , 9 ( 8 ): 771 .

ANEKWE I M S , NYEMBE N , NQAKALA L C , et al . Sustainable fuels: Lower alcohols perspective [J ] . Environmental Progress & Sustainable Energy , 2023 , 42 ( 6 ).

KNITTEL C R , MEISELMAN B S , STOCK J H . The pass-through of rin prices to wholesale and retail fuels under the renewable fuel standard [J ] . Journal of the Association of Environmental and Resource Economists , 2017 , 4 ( 4 ): 1081 - 1119 .

EGGERT H , GREAKER M . Promoting second generation biofuels: does the first generation pave the road? [J ] . Energies , 2014 , 7 ( 7 ): 4430 - 4445 .

DANIELL J , KÖPKE M , SIMPSON S . Commercial biomass syngas fermentation [J ] . Energies , 2012 , 5 ( 12 ): 5372 - 5417 .

巨鹏生物发展历史 [EB/OL ] . http://cn.jupengbio.com/history http://cn.jupengbio.com/history .

塞纳达生物01 [EB/OL ] . https://baijiahao.baidu.com/s?id=1789206954065696710&wfr=spider&for=pc https://baijiahao.baidu.com/s?id=1789206954065696710&wfr=spider&for=pc .

食气生化 [EB/OL ] . https://baijiahao.baidu.com/s?id=1792216666824871730&wfr=spider&for=pc https://baijiahao.baidu.com/s?id=1792216666824871730&wfr=spider&for=pc .

郭姝媛 , 王博 , 于涛 , 等 . 微生物中一碳代谢网络构建的进展与挑战 [J ] . 合成生物学 , 2022 , 3 ( 1 ): 116 - 137 .

GUO S Y , WANG B , YU T , et al . Developing C1-based metabolic network in methylotrophy for biotransformation [J ] . Synthetic Biology Journal , 2022 , 3 ( 1 ): 116 - 137 .

禹伟 , 高教琪 , 周雍进 . 一碳生物转化合成有机酸的研究进展 [J ] . 合成生物学 , 2024 , 5 ( 5 ): 1169 - 1188 .

YU W , GAO J Q , ZHOU Y J . Bioconversion of one carbon feedstocks for producing organic acids [J ] . Synthetic Biology Journal , 2024 , 5 ( 5 ): 1169 - 1188 .

赵亮 , 李福利 , 刘自勇 , 等 . 生物转化一碳化合物原料产油脂与单细胞蛋白研究进展 [J ] . 合成生物学 , 2024 , 5 ( 6 ): 1300 - 1318 .

ZHAO L , LI F L , LIU Z Y . Progress in biomanufacturing of lipids and single cell protein from one-carbon compounds [J ] . Synthetic Biology Journal , 2024 , 5 ( 6 ): 1300 - 1318 .

叶伟 , 姜卫红 , 顾阳 . 二氧化碳微生物转化与体外酶催化体系研究进展 [J ] . 合成生物学 , 2023 , 4 ( 6 ): 1223 - 1245 .

YE W , JIANG W H , GU Y . Microbial conversion and in vitro enzymatic catalysis for carbon dioxide utilization: a review [J ] . Synthetic Biology Journal , 2023 , 4 ( 6 ): 1223 - 1245 .

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Regulating the commercialization of cell-cultured meat: practices in selected jurisdictions and their implications for China

The legal issues about commercialization of food products employing synthetic biology strategies

Related Author

LI Yujuan

FU Xiongfei

DU Li

WANG Meng

Xiongfei FU

Yujuan LI

Li DU

Related Institution

Faculty of Law， University of Macau， Macau SAR

CAS Key Laboratory of Quantitative Engineering Biology， Shenzhen Institute of Synthetic Biology， Shenzhen Institutes of Advanced Technology， Chinese Academy of Sciences

⁰