酿酒酵母使能技术在化妆品原料合成中的应用
Enabling technology for the biosynthesis of cosmetic raw materials with
Saccharomyces cerevisiae - 合成生物学 2025年6卷第2期 页码：233-253
- 作者机构：
1.浙江大学化学工程与生物工程学院，生物质化工教育部重点实验室，生物基运输燃料技术全国重点实验室，浙江 杭州 310027
2.浙江大学杭州国际科创中心，浙江 杭州 310000
- 作者简介：
[ "左一萌（1997—），女，博士研究生。研究方向为植物天然产物合成生物学。E-mail：ymzuo@zju.edu.cn" ]
[ "张姣姣（1994—），女，博士研究生。研究方向为植物天然产物合成生物学。E-mail：L231459@zju.edu.cn" ]
[ "连佳长（1984—），男，博士，研究员。研究方向为合成生物学的相关研究。E-mail：jzlian@zju.edu.cn" ]
- 基金信息：浙江省属高校基本科研业务费专项资金(226-2023-00015);国家自然科学基金(22278361;32200052;32300053;22478341)
DOI：10.12211/2096-8280.2024-070
中图分类号： Q812收稿：2024-09-02，
修回：2024-11-04，
纸质出版：2025-04-30
- 稿件说明：
移动端阅览
左一萌， 张姣姣， 连佳长. 酿酒酵母使能技术在化妆品原料合成中的应用［J］. 合成生物学， 2025， 6（2）： 233-253
ZUO Yimeng， ZHANG Jiaojiao， LIAN Jiazhang. Enabling technology for the biosynthesis of cosmetic raw materials with Saccharomyces cerevisiae［J］. Synthetic Biology Journal， 2025， 6（2）： 233-253
左一萌， 张姣姣， 连佳长. 酿酒酵母使能技术在化妆品原料合成中的应用［J］. 合成生物学， 2025， 6（2）： 233-253 DOI： 10.12211/2096-8280.2024-070.
ZUO Yimeng， ZHANG Jiaojiao， LIAN Jiazhang. Enabling technology for the biosynthesis of cosmetic raw materials with Saccharomyces cerevisiae［J］. Synthetic Biology Journal， 2025， 6（2）： 233-253 DOI： 10.12211/2096-8280.2024-070.
摘要
伴随消费者对化妆品的需求急剧增长，化妆品原料市场同步扩张。化妆品原料作为化妆品的核心成分，不仅承载着化妆品的主体功效和产品竞争力，同时对化妆品的安全也至关重要。合成生物学是以工程化设计为理念，利用基因编辑技术、计算机模拟技术和生物工程等技术对生物体进行有目标的设计、改造乃至重新合成的一门新兴交叉融合性学科。合成生物学的进步使微生物宿主能够以高效、具有成本竞争力和安全的方式合成有价值的天然产物。随着合成生物学的不断发展，酿酒酵母作为一种重要的微生物底盘细胞，在化妆品原料合成中的应用日益广泛。构建酿酒酵母细胞工厂异源生物合成化妆品原料作为一种有效的替代方案，具有环保、可持续的优点，可以减少对传统物理提取法的依赖以及规避化学合成法的污染问题。本文综述了酿酒酵母基因编辑技术的发展及其在化妆品原料生物合成途径构建中的关键作用，总结了基因多拷贝整合、区室化工程、转运工程、人工多细胞体系等代谢工程策略在化妆品原料酿酒酵母细胞工厂优化中的应用，并进一步从萜类、维生素类、多酚类、蛋白质与氨基酸类等不同类别的化妆品活性成分出发，阐述了酿酒酵母细胞工厂生物合成化妆品原料的最新进展。虽然酿酒酵母在化妆品原料大规模生产方面具有巨大潜能与优势，然而目前仍面临诸如产品生物合成途径未完全解析、生物合成水平较为低下、分离纯化困难等一系列挑战。未来，结合人工智能、机器学习等手段有望开发更为高效的基因编辑工具并应用于酿酒酵母细胞工厂的优化与化妆品原料成分的合成中，为化妆品行业的可持续发展提供理论支持和实践指导。
Abstract
With the rapid growth of consumption in cosmetics
demand for their raw materials is expanding correspondingly
which not only drive the efficacy and product competitiveness but are also crucial for ensuring safety. Synthetic biology
an emerging interdisciplinary field based on engineering principles
leverages gene editing
computer simulation
and bioengineering technologies to design
modify
and even resynthesize organisms through rational strategies.
Saccharomyces cerevisiae
an important microbial platform
is increasingly used in the production of cosmetic raw materials. Constructing
S. cerevisiae
cell factories for the heterologous biosynthesis of cosmetic ingredients presents an eco-friendly and sustainable alternative to traditional plant extraction and chemical synthesis
addressing both environmental concern and resource limitation. In this article
we review the development of gene editing technology and its key role in constructing biosynthetic pathways for the production of cosmetic raw materials with
S. cerevisiae
. We also summarize the application of metabolic engineering strategies such as multi-copy gene integration
compartmentalization
transporter engineering
and multicellular system in the optimization of
S. cerevisiae
cell factories. Moreover
we present the latest progress in the biosynthesis of different cosmetic active ingredients with
S. cerevisiae
cell factories
such as terpenes
vitamins
polyphenols
proteins and amino acids. While the potential and advantages of using
S. cerevisiae
for large-scale production of cosmetic raw materials are significant
a series of challenges remain
including incomplete biosynthetic pathway analysis
low biosynthesis yield
and low yield with the separation and purification. Looking ahead
the integration of artificial intelligence
machine learning
and other advanced technologies is expected to establish more efficient gene editing tools for the optimization of yeast cell factories and the biosynthesis of cosmetic raw materials
providing technical support and practical guidance for the sustainable development of the cosmetics industry.
2
关键词
Keywords
references
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