Core Elements and Key Technologies of ivBT-based Glycan Synthesis

PEI Caixia; LI Jianjun; DU Yuguang

doi:10.12211/2096-8280.2025-064

您当前的位置：

首页 >

文章列表页 >

Core Elements and Key Technologies of ivBT-based Glycan Synthesis

更新时间：2025-08-13

- Core Elements and Key Technologies of ivBT-based Glycan Synthesis
- Synthetic Biology Journal Pages: 1-19(2025)
- 作者机构：
  
  1.中国科学院过程工程研究所，生物药制备与递送全国重点实验室，北京 100190
  2.中国科学院过程工程研究所，国家生化工程技术研究中心（北京），北京 100190
- 作者简介：
- 基金信息：
- DOI：10.12211/2096-8280.2025-064
  CLC： Q814.9
- Received：23 June 2025，
  
  Revised：2025-08-10，
  
  Online First：13 August 2025，
- 稿件说明：
移动端阅览
裴彩霞，李建军，杜昱光. 糖质ivBT生物合成的核心元件及关键技术［J］. 合成生物学， 2025， 6. DOI： 10.12211/2096-8280.2025-064

PEI Caixia， LI Jianjun， DU Yuguang. Core Elements and Key Technologies of ivBT-based Glycan Synthesis［J］. Synthetic Biology Journal， 2025， 6. DOI： 10.12211/2096-8280.2025-064
裴彩霞，李建军，杜昱光. 糖质ivBT生物合成的核心元件及关键技术［J］. 合成生物学， 2025， 6. DOI： 10.12211/2096-8280.2025-064 DOI：

PEI Caixia， LI Jianjun， DU Yuguang. Core Elements and Key Technologies of ivBT-based Glycan Synthesis［J］. Synthetic Biology Journal， 2025， 6. DOI： 10.12211/2096-8280.2025-064 DOI：

摘要

糖质（glycans）是构成生命的基本生物分子，在生物过程中发挥重要作用，与人类健康和疾病密切相关。由于糖质结构的复杂性，糖质所携带的密码信息大大超过核酸及蛋白质等任何其它生物大分子。为了破译糖质携带的密码信息，就需要获得一定量的、结构明确的糖质。合成仍然是获得糖质的最佳方式。体外生物转化（

in vitro

biotransformation， ivBT）是一个基于体外多酶催化的新型工业生物制造技术，由酶、辅酶等元件体外重构生化反应途径，实现目标产物的高效合成，正逐渐发展成为合成糖质的一种重要技术。本文综述了ivBT在糖质合成中的应用、ivBT糖质合成相关的糖核苷合成、糖基转移、NTP再生等核心元件及酶的固定化、微流控、酶促自动化合成、动力学模拟、离子液体等关键技术，展望了糖质ivBT合成相关的酶的协同性、特殊重组酶生产成本的降低、仿生辅酶的循环再生、多酶复合体的应用、硫酸化糖质的合成、AI辅助的反应条件优化等发展方向，旨在为糖质ivBT合成的未来发展提供指导。

Abstract

Glycans are the fundamental biomolecules that make up life and play important roles in biological processes

including cell growth and proliferation

immune responses

angiogenesis and tumor cell metastasis

toxin interaction

protein folding and degradation

cell-cell communications

and cell-pathogen interactions

and are closely related to human health and disease such as occurrence

development

and metastasis of tumor

inflammation

viral or bacterial infections

etc. Due to their structural complexity arising from the number of chemically similar (and often isomeric) monosaccharide building blocks

the position and orientation of glycosidic linkages

branching

and non-template-driven biosynthesis

the coding information they carry far exceeds that of any other biological macromolecules such as nucleic acids and proteins

which are generally linear structures and biosynthesized based on templates. To decipher the code information carried by glycans

it is necessary to obtain some structurally defined pure glycans. Since it is not possible to separate and purify glycans with defined structures from natural environment

synthesis remains the best approach to obtaining glycans. In vitro biotransformation (ivBT)

a novel industrial biomanufacturing platform based on multi-enzyme catalysis in vitro

has become an essential approach to obtain high value-added products due to its appealing advantages in rapid construction of nonnatural enzymatic pathways

higher product yields

faster reaction rates

and better tolerances to toxic compounds. ivBT is gradually being developed into an important means of glycan synthesis. This article reviews application of ivBT in glycan synthesis

the core elements such as synthesis of sugar-nucleotides

glycosyl transfer and NTP regeneration

and key technologies of ivBT-based glycan synthesis like enzyme immobilization

microfluidics

enzyme-mediated automatic synthesis

dynamic simulation

and ion liquids

aiming to provide guidance for its future development

including synergism of multi-enzyme

reduction of production costs for special recombinant enzymes

regeneration of biomimetic coenzymes

application of complexes of multi-enzyme

ivBT synthesis of sulfated glycans and AI-assisted optimization of reaction conditions of ivBT synthesis

etc.

关键词

Keywords

references

VARKI A , CUMMINGS R D , ESKO J D , et al . Essentials of Glycobiology . Cold Spring Harbor (NY) . 2022 .

Transforming Glycoscience: A Roadmap for the Future . Washington (DC) . 2012 .

SEEBERGER P H . The logic of automated glycan assembly [J ] . Accounts of Chemical Research , 2015 , 48 ( 5 ): 1450 - 1463 .

BOLTJE T J , BUSKAS T , BOONS G J . Opportunities and challenges in synthetic oligosaccharide and glycoconjugate research [J ] . Nature Chemistry , 2009 , 1 ( 8 ): 611 - 622 .

HSU C H , HUNG S C , WU C Y , et al . Toward automated oligosaccharide synthesis [J ] . Angewandte Chemie International Edition , 2011 , 50 ( 50 ): 11872 - 11923 .

YU H , CHRN X . One-pot multienzyme (OPME) systems for chemoenzymatic synthesis of carbohydrates [J ] . Organic & Biomolecular Chemistry ,, 2016 , 14 ( 10 ): 2809 - 2818 .

MUTHANA S , CAO H , CHEN X . Recent progress in chemical and chemoenzymatic synthesis of carbohydrates [J ] . Current Opinions in Chemical Biology , 2009 , 13 ( 5-6 ): 573 - 581 .

ZHANG Y H P , MYUNGy S , YOU C , et al . Toward low-cost biomanufacturing through in vitro synthetic biology: bottom-up design [J ] . Journal of Materials Chemistry , 2011 , 21 ( 47 ): 18877 - 18886 .

LOPEZ-GALLEGO F , SCHMIDT-DANNERT C . Multi-enzymatic synthesis [J ] . Current Opinions in Chemical Biology , 2010 , 14 ( 2 ): 174 - 183 .

VASIC-RACKI D , FINDRIK Z , PRESCKI A V . Modelling as a tool of enzyme reaction engineering for enzyme reactor development [J ] . Applied Microbiology and Biotechnology , 2011 , 91 ( 4 ): 845 - 856 .

VILKHOVOY M , ADHIKARI A , VADHIN S , et al . The Evolution of Cell Free Biomanufacturing [J ] . Processes , 2020 , 8 ( 6 ): 675 .

JAROENTOMEECHAI T , TAW M N , Li M , et al . Cell-Free Synthetic Glycobiology: Designing and Engineering Glycomolecules Outside of Living Cells [J ] . Frontiers in Chemistry , 2020 , 8 : 645 .

LUO S , LIN P P , NIEH L-Y , et al . A cell-free self-replenishing CO2-fixing system [J ] . Nature Catalysis , 2022 , 5 ( 2 ): 154 - 162 .

ZHANG Y H . Production of biocommodities and bioelectricity by cell-free synthetic enzymatic pathway biotransformations: challenges and opportunities [J ] . Biotechnology and Bioengineering , 2010 , 105 ( 4 ): 663 - 677 .

ZHANG Y H P J , ZHU Z , YOU C , at al . In Vitro BioTransformation (ivBT): Definitions, Opportunities, and Challenges [J ] . Synthtic Biology and Engineering , 2023 , 1 ( 2 ): 10013

石婷 , 宋展 , 宋世怡 , 等 , 体外生物转化(ivBT) : 生物制造的新前沿 [J ] , 合成生物学 , 2024 , 5 ( 6 ): 1437 - 1460

SHI T , SONG Z , SONG S , et al . In vitro BioTransformation (ivBT): a new frontier of. industrial biomanufacturing [J ] . Synthetic Biology Journal , 2024 , 5 ( 6 ): 1437 - 1460

BHOSALE S H , RAO M B , DESHPANDE V V . Molecular and industrial aspects of glucose isomerase [J ] . Microbiological reviews , 1996 , 60 ( 2 ): 280 - 300 .

DEMAIN A L . Pickles, pectin, and penicillin [J ] . Annual Review Microbiology , 2004 , 58 : 1 - 42 .

TAHERZADEH M J , KARIMI K J B . Enzyme-based hydrolysis processes for ethanol from lignocellulosic materials: A review [J ] . BioResources , 2007 , 2 ( 4 ): 707 - 738 .

WICHMANN R , VASIC-RACKI D . Cofactor regeneration at the lab scale [J ] . Advances in Biochemical Engineering/Biotechnology , 2005 , 92 : 225 - 260 .

KOELLER K M , WONG C H . Synthesis of complex carbohydrates and glycoconjugates: enzyme-based and programmable one-pot strategies [J ] . Chemical Reviews , 2000 , 100 ( 12 ): 4465 - 4494 .

THIBODEAUX C J , MELANCON C E , LIU H W . Unusual sugar biosynthesis and natural product glycodiversification [J ] . Nature , 2007 , 446 ( 7139 ): 1008 - 1016 .

THIBODEAUX C J , MELANÇON C E , LIU H W . Natural‐Product Sugar Biosynthesis and Enzymatic Glycodiversification [J ] . Angewandte Chemie International Edition , 2008 , 47 ( 51 ): 9814 - 9859 .

YOU C , CHENh H , MYUNG S , et al . Enzymatic transformation of nonfood biomass to starch [J ] . Proceedings of the National Academy of Sciences of the United States of America , 2013 , 110 ( 18 ): 7182 - 7187 .

DURDA V-R . History of industrial biotransformations-dreamand realities [M ] . Weinheim : Wiley-VCH, KGaA , 2006 .

FRANCE S P , HEPWORTH L J , TURNER N J , et al . Constructing Biocatalytic Cascades: In Vitro and in Vivo Approaches to de Novo Multi-Enzyme Pathways [J ] . ACS Catalysis , 2017 , 7 ( 1 ): 710 - 724 .

DING Q B , OU L . NTP Regeneration and its Application in the Biosynthesis of Nucleotides and their Derivatives [J ] . Current Pharmaceutical Design , 2017 , 23 ( 45 ): 6936 – 6947 .

YANG J , ZHANG T , TIAN C , et al . Multi-enzyme systems and recombinant cells for synthesis of valuable saccharides: Advances and perspectives [J ] . Biotechnology Advances , 2019 , 37 ( 7 ): 107406 .

YE J , LIU X PENG P , et al . Diversity-Oriented Enzymatic Modular Assembly of ABO Histo-blood Group Antigens [J ] . ACS Catalysis , 2016 , 6 ( 12 ): 8140 - 8144 .

WANG S , ZHANG Q , CHEN C , et al . Facile Chemoenzymatic Synthesis of O-Mannosyl Glycans [J ] . Angewandte Chemie International Edition , 2018 , 57 ( 30 ): 9268 - 9273 .

MENG C , SASMAL A , ZHANG Y , et al . Chemoenzymatic Assembly of Mammalian O-Mannose Glycans [J ] . Angewandte Chemie International Edition , 2018 , 57 ( 29 ): 9003 - 9007 .

LI Y , LI Y , GUO Y , et al . Enzymatic modular synthesis of asymmetrically branched human milk oligosaccharides [J ] . Carbohydrate Polymers , 2024 , 333 : 121908 .

BAI Y , AGRAHARI A K , ZHANG L , et al . EASyMap-Guided Stepwise One-Pot Multienzyme (StOPMe) Synthesis and Multiplex Assays Identify Functional Tetraose-Core-Human Milk Oligosaccharides [J ] . JACS Au , 2025 , 5 ( 2 ): 822 - 837 .

TSAI T I , LEE H Y , CHANG S H , et al . Effective sugar nucleotide regeneration for the large-scale enzymatic synthesis of Globo H and SSEA4 [J ] . Journal of the American Chemical Society , 2013 , 135 ( 39 ): 14831 - 14839 .

TSENG H W , TSENG H K , OOI K E , et al . Controllable Enzymatic Synthesis of Natural Asymmetric Human Milk Oligosaccharides [J ] . JACS Au , 2024 , 4 ( 11 ): 4496 - 4506 .

REXER T F T , SCHILDBACH A , KLAPPROTH J , et al . One pot synthesis of GDP-mannose by a multi-enzyme cascade for enzymatic assembly of lipid-linked oligosaccharides [J ] . Biotechnology and Bioengineering , 2018 , 115 ( 1 ): 192 - 205 .

DU Z , LIU Z , TAN Y , et al . Lacto-N-biose synthesis via a modular enzymatic cascade with ATP regeneration [J ] . iScience , 2021 , 24 ( 3 ): 102236 .

JIAO R , ZHANG L , YOU R , et al . Efficient and Cost-Effective Synthesis of N-Acetyllactosamine by Sequential Modular Enzymatic Cascade Reactions Involving NTP Regeneration [J ] . Journal of Agricultural and Food Chemistry , 2024 , 72 ( 50 ): 28060 - 28071 .

JIAO R , PENG X , WANG B , DU Y , QIAN E W , LI J . Highly Efficient and Economical One-Pot Two-Step Multienzymatic Synthesis of 6′/3′-Sialyllactosamine from In Situ-Produced N-Acetyllactosamine [J ] . Journal of Agricultural and Food Chemistry , 2025 , 73 ( 23 ): 14444 - 14452 .

FENG Y , YAO M , WANG Y , et al . Advances in engineering UDP-sugar supply for recombinant biosynthesis of glycosides in microbes [J ] . Biotechnology Advances , 2020 , 41 : 107538 .

ZHUANG J D , SHI J M , HONG C C , et al . Engineering Bifunctional Galactokinase/Uridyltransferase Chimera for Enhanced UDP-d-Xylose Production [J ] . JACS Au , 2024 , 4 ( 7 ): 2557 - 2563 .

SHI J , WU T , ZHOU H , et al . Substrate promiscuities of a bacterial galactokinase and a glucose-1-phosphate uridyltransferase enable xylose salvaging [J ] . Green Chemistry , 2022 , 24 ( 9 ): 3717 - 3722 .

WEIJERS C A , FRANSSEN M C , VISSER G M . Glycosyltransferase-catalyzed synthesis of bioactive oligosaccharides [J ] . Biotechnology Advances , 2008 , 26 ( 5 ): 436 - 456 .

LAIRSON L L , HENRISSAT B , DAVIES G J , et al . Glycosyltransferases: structures, functions, and mechanisms [J ] . Annual Review of Biochemistry , 2008 , 77 : 521 - 555 .

SCHMALTZ R M , HANSON S R , WONG C H . Enzymes in the Synthesis of Glycoconjugates [J ] . Chemical Reviews , 2011 , 111 ( 7 ): 4259 - 4307 .

REXER T , LAAF D , GOTTSCHALK J , et al . Enzymatic Synthesis of Glycans and Glycoconjugates [J ] . Advances in Biochemical Engineering/Biotechnology , 2021 , 175 : 231 - 280 .

CHANG L , CUI H , LIU W , et al . Electrodriven ATP Synthesis via a Reconstructed Thylakoid Membrane [J ] . Angewandte Chemie International Edition , 2025 , 64 ( 6 ): e202421120 .

LUO S S , ADAM D , GIAVERI S , et al . ATP production from electricity with a new-to-nature electrobiological module [J ] . Joule , 2023 , 7 ( 8 ): 1745 - 1758 .

RUCCOLO S , BRITO G , CHRISTENSEN M , et al . Electrochemical Recycling of Adenosine Triphosphate in Biocatalytic Reaction Cascades [J ] . Journal of Agricultural and Food Chemistry , 2022 , 144 ( 49 ): 22582 - 22588 .

LEE J H , CHUNG S W , LEE H J , et al . Optimization of the enzymatic one pot reaction for the synthesis of uridine 5'-diphosphogalactose [J ] . Bioprocess and Biosystems Engineering , 2010 , 33 ( 1 ): 71 - 78 .

LEE S G , LEE J O , YI J K , et al . Production of cytidine 5′-monophosphate N-acetylneuraminic acid using recombinant as a biocatalyst [J ] . Biotechnology and Bioengineering , 2002 , 80 ( 5 ): 516 - 524 .

ANDEXER J N , RICHTER M . Emerging enzymes for ATP regeneration in biocatalytic processes [J ] . Chembiochem , 2015 , 16 ( 3 ): 380 - 386 .

NAHALKA J , PATOPRSTY V . Enzymatic synthesis of sialylation substrates powered by a novel polyphosphate kinase (PPK3) [J ] Organic & Biomolecular Chemistry , 2009 , 7 ( 9 ): 1778 - 1780 .

Cai T , Sun H , Qiao J , et al . Cell-free chemoenzymatic starch synthesis from carbon dioxide [J ] . Science , 2021 , 373 ( 6562 ): 1523 - 1527 .

LIU Z , ZHANG J , CHEN X , et al . Combined biosynthetic pathway for de novo production of UDP-galactose: catalysis with multiple enzymes immobilized on agarose beads [J ] . Chembiochem , 2002 , 3 ( 4 ): 348 - 355 .

ZHANG J , WU B , ZHANG Y , et al . Creatine phosphate--creatine kinase in enzymatic synthesis of glycoconjugates [J ] . Organic Letters , 2003 , 5 ( 15 ): 2583 - 2586 .

WOHLGEMUTH R . Biocatalysis-key to sustainable industrial chemistry [J ] . Current Opinion in Biotechnology , 2010 , 21 ( 6 ): 713 - 724 .

STEPANKOVA V , BIDMANOVA S , KOUDELAKOVA T , et al . Strategies for Stabilization of Enzymes in Organic Solvents [J ] . ACS Catalysis , 2013 , 3 ( 12 ): 2823 - 2836 .

HANEFELD U , GARDOSSI L , MAGNER E . Understanding enzyme immobilisation [J ] . Chemical Society Reviews , 2009 , 38 ( 2 ): 453 - 468 .

MATEO C , PALOMO J M , FERNANDEZ-LORENTE G , et al . Improvement of enzyme activity, stability and selectivity via immobilization techniques [J ] . Enzyme and Microbial Technology , 2007 , 40 ( 6 ): 1451 - 1463 .

BARBOSA O , ORTIZ C , BERENGUER-MURCIA A , et al . Strategies for the one-step immobilization-purification of enzymes as industrial biocatalysts [J ] . Biotechnology Advances , 2015 , 33 ( 5 ): 435 - 456 .

JI Q , WANG B , TAN J , et al . Immobilized multienzymatic systems for catalysis of cascade reactions [J ] . Process Biochemistry , 2016 , 51 ( 9 ): 1193 - 1203 .

CHEN X , FANG J , ZHANG J , et al . Sugar nucleotide regeneration beads (superbeads): a versatile tool for the practical synthesis of oligosaccharides [J ] . Journal of the American Chemical Society , 2001 , 123 ( 9 ): 2081 - 2082 .

LI Z , LIU X , NAKANISHI H , et al . Encapsulation of Mannose-6-phosphate Isomerase in Yeast Spores and Its Application in l-Ribose Production [J ] . Journal of Agricultural and Food Chemistry , 2020 , 68 ( 25 ): 6892 - 6899 .

LI Z , LI Y , DUAN S , et al . Bioconversion of D-glucose to D-psicose with immobilized D-xylose isomerase and D-psicose 3-epimerase on Saccharomyces cerevisiae spores [J ] . Journal of Industrial Microbiology and Biotechnology , 2015 , 42 ( 8 ): 1117 - 1128 .

CHAO Q , LI T L , JIA J X , et al . Spore-Encapsulating Glycosyltransferase Catalysis Tandem Reactions: Facile Chemoenzymatic Synthesis of Complex Human Glycans [J ] . ACS Catalysis , 2022 , 12 ( 5 ): 3181 - 3188 .

LIANG W , WIED P , CARRARO F , et al . Metal-Organic Framework-Based Enzyme Biocomposites [J ] . Chemical Reviews , 2021 , 121 ( 3 ): 1077 - 1129 .

BAILEY J B , ZHANG L , CHIONG J A , et al . Synthetic Modularity of Protein-Metal-Organic Frameworks [J ] . Journal of the American Chemical Society , 2017 , 139 ( 24 ): 8160 - 8166 .

PATIL P D , GARGATE N , TIWARI M S , et al . Designable metal-organic frameworks for enzyme immobilization: The reality of controlled architecture [J ] . Chemical Engineering Journal , 2025 , 508 , 160994 .

SHA F R , WANG X L , KIRLIKOVALI K O , et al . Enhancing Biocatalysis: Metal-Organic Frameworks as Multifunctional Enzyme Hosts [J ] . Accounts of Chemical Research , 2024 , 57 ( 24 ): 3500 - 3511 .

ZHENG J , XU H , LI B , et al . Spatially Segregated MOF Bioreactor Enables Versatile Modular Glycoenzyme Assembly for Hierarchical Glycan Library Construction [J ] . ACS Applied Materials & Interfaces , 2023 , 15 ( 16 ): 19807 - 19816 .

PENG X , PEI C , QIAN E W , et al . Co-immobilization of a bi-enzymatic cascade into hierarchically porous MIL-53 for efficient 6'-sialyllactose production [J ] . Nanoscale , 2024 , 16 ( 31 ): 14932 - 14939 .

Han P , Wang X , Li Y , et al . Synthesis of a Healthy Sweetener d-Tagatose from Starch Catalyzed by Semiartificial Cell Factories [J ] . Journal of Agricultural and Food Chemistry , 2023 , 71 ( 8 ): 3813 - 3820 .

林炳承 . 微纳流控芯片实验室 [M ] . 2013 .

MARTIN J G , GUPTA M , XU Y , et al . Toward an artificial Golgi: redesigning the biological activities of heparan sulfate on a digital microfluidic chip [J ] . Journal of the American Chemical Society , 2009 , 131 ( 31 ): 11041 - 11048 .

GONG M , LI T , WU L , et al . Liquid-Phase and Ultrahigh-Frequency-Acoustofluidics-Based Solid-Phase Synthesis of Biotin-Tagged 6′/3′-Sialyl-N-Acetylglucosamine by Sequential One-Pot Multienzyme System [J ] . Catalysts , 2020 , 10 ( 11 ): 1347 .

SUN Y , WU Y , MA D , et al . Digital microfluidics-engaged automated enzymatic degradation and synthesis of oligosaccharides [J ] . Frontiers in Bioengineering and Biotechnology , 2023 , 11 : 1201300 .

WU Y , SUN Y , PEI C , et al . Automated chemoenzymatic modular synthesis of human milk oligosaccharides on a digital microfluidic platform [J ] . RSC Advances , 2024 , 14 ( 25 ): 17397 - 17405 .

WEN L , EDMUNDS G , GIBBONS C , et al . Toward Automated Enzymatic Synthesis of Oligosaccharides [J ] . Chemical Reviews , 2018 , 118 ( 17 ): 8151 - 8187 .

PLANTE O J , PALMACCI E R , SEEBERGER P H . Automated solid-phase synthesis of oligosaccharides [J ] . Science , 2001 , 291 ( 5508 ): 1523 - 1527 .

YAO W , XIONG D , YANG Y , et al . Automated solution-phase multiplicative synthesis of complex glycans up to a 1,080-mer [J ] . Nature Synthesis , 2022 , 1 : 854 - 863 .

SCHUSTER M , WANG P , PAULSON J C , et al . Solid-Phase Chemical-Enzymatic Synthesis of Glycopeptides and Oligosaccharides [J ] . Journal of the American Chemical Society , 1994 , 116 ( 3 ): 1135 - 1136 .

HALCOMB R L , HUANG H , WONG C-H . Solution- and Solid-Phase Synthesis of Inhibitors of H. pylori Attachment and E-Selectin-Mediated Leukocyte Adhesion [J ] . Journal of the American Chemical Society , 1994 , 116 ( 25 ): 11315 - 11322 .

MATSUSHITA T , NAGASHIMA I , FUMOTO M , et al . Artificial Golgi apparatus: globular protein-like dendrimer facilitates fully automated enzymatic glycan synthesis [J ] . Journal of the American Chemical Society , 2010 , 132 ( 46 ): 16651 - 16656 .

ZHANG J , CHEN C , GADI M R , et al . Machine-Driven Enzymatic Oligosaccharide Synthesis by Using a Peptide Synthesizer [J ] . Angewandte Chemie International Edition , 2018 , 57 ( 51 ): 16638 - 16642 .

ZHANG J , LIU D , SAIKAM V , et al . Machine-Driven Chemoenzymatic Synthesis of Glycopeptide [J ] . Angewandte Chemie International Edition , 2020 , 59 ( 45 ): 19825 - 19829 .

LI T , LIU L , WEI N , et al . An automated platform for the enzyme-mediated assembly of complex oligosaccharides [J ] . Nature Chemistry , 2019 , 11 ( 3 ): 229 - 236 .

MAHOUR R , KLAPPROTH J , REXER T F T , et al . Establishment of a five-enzyme cell-free cascade for the synthesis of uridine diphosphate N-acetylglucosamine [J ] . Journal of Biotechnology , 2018 , 283 : 120 - 129 .

SCHELCH S , EIBINGER M , GROSS Belduma S , et al . Engineering analysis of multienzyme cascade reactions for 3'-sialyllactose synthesis [J ] . Biotechnology and Bioengineering , 2021 , 118 ( 11 ): 4290 - 4304 .

DONG K , LIU X , DONG H , et al . Multiscale Studies on Ionic Liquids [J ] . Chemical Reviews , 2017 , 117 ( 10 ): 6636 - 6695 .

ANGELL C A , ANSARI Y , ZHAO Z . Ionic liquids: past, present and future [J ] . Faraday Discussions , 2012 , 154 : 9 - 27 .

ITOH T . Ionic Liquids as Tool to Improve Enzymatic Organic Synthesis [J ] . Chemical Reviews , 2017 , 117 ( 15 ): 10567 - 10607 .

LI Z , HAN Q , WANG K , et al . Ionic liquids as a tunable solvent and modifier for biocatalysis [J ] . Catalysis Reviews , 2024 , 66 ( 2 ): 484 - 530 .

JI X L , XUE Y J , LI Z , et al . Ionozyme: ionic liquids as solvent and stabilizer for efficient bioactivation of CO2 [J ] . Green Chemistry , 2021 , 23 ( 18 ): 6990 - 7000 .

LI Z , WANG K , XUE Y J , et al . Ionozymes for Efficient Synthesis of Cadaverine: Offering a Sustainable Way for Bio-nylon 5X Production [J ] . ACS Sustainable Chemistry & Engineering , 2023 , 11 ( 10 ): 4197 - 4208 .

LANG M , KAMRAT T , NIDETZKY B . Influence of ionic liquid cosolvent on transgalactosylation reactions catalyzed by thermostable β-glycosylhydrolase celB from [J ] . Biotechnology and Bioengineering , 2006 , 95 ( 6 ): 1093 - 1100 .

SANDOVAL M , CORTÉS A , CIVERA C , et al . Efficient and selective enzymatic synthesis of N-acetyl-lactosamine in ionic liquid: a rational explanation [J ] . RSC Advances , 2012 , 2 ( 15 ): 6306 - 6314 .

BAYÓN C , CORTÉS A , BERENGUER J , et al . Highly efficient enzymatic synthesis of Galβ-(1→3)-GalNAc and Galβ-(1→3)-GlcNAc in ionic liquids [J ] . Tetrahedron , 2013 , 69 ( 24 ): 4973 - 4978 .

PATEL M S , NEMERIA N S , FUREY W , et al . The pyruvate dehydrogenase complexes: structure-based function and regulation [J ] . The Journal of biological chemistry , 2014 , 289 ( 24 ): 16615 - 16623 .

PAPAGEORGIOU A C . Structural Characterization of Multienzyme Assemblies: An Overview [J ] . Methods in molecular biology , 2022 , 2487 : 51 - 72 .

QU J , CAO S , WEI Q , et al . Synthetic Multienzyme Complexes, Catalytic Nanomachineries for Cascade Biosynthesis In Vivo [J ] . ACS Nano , 2019 , 13 ( 9 ): 9895 - 9906 .

OVADI J , SRERE P A . Metabolic consequences of enzyme interactions [J ] . Cell Biochemistry and Function , 1996 , 14 ( 4 ): 249 - 258 .

HWANG E T , LEE S . Multienzymatic Cascade Reactions via Enzyme Complex by Immobilization [J ] . ACS Catalysis , 2019 , 9 ( 5 ): 4402 - 4425 .

XU R R , WANG Y , HUANG H , et al . Closed-Loop System Driven by ADP Phosphorylation from Pyrophosphate Affords Equimolar Transformation of ATP to 3′-Phosphoadenosine-5′- phosphosulfate [J ] . ACS Catalysis , 2021 , 11 ( 16 ): 10405 - 10415 .

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

No data

Related Author

No data

Related Institution

No data

⁰