1.中国科学院微生物研究所微生物生理与代谢工程研究室,北京 100101
2.中国科学院大学,北京 100049
王丽敏(1978年8月—),女,项目研究员,中国科学院微生物研究所特聘研究骨干。研究方向为芽孢杆菌及非模式酵母菌的化学品合成与代谢工程改造,主要包括可降解材料聚乳酸合成单体L-乳酸和D-乳酸,以及高分子生物聚合物的生物合成研究。
收稿:2026-09-18,
修回:2026-11-01,
网络首发:2026-11-04,
移动端阅览
王丽敏, 张莉敏, 于波. 耐酸微生物底盘细胞创制及应用[J]. 合成生物学, 2026, 7. DOI: 10.12211/2096-8280.2026-107
Wang L-min, Zhang Limin, Yu Bo. Development and Application of Acid-Tolerant Microbial Chassis Cells[J]. Synthetic Biology Journal, 2026, 7. DOI: 10.12211/2096-8280.2026-107
在合成生物学的推动下,微生物制造作为连接基础研究与工业应用的桥梁,已在多种产品的规模化生产中展现出巨大潜力,成为当前生物制造领域备受瞩目的研究方向。与传统微生物相比,极端微生物因其在胁迫条件下优异的生长与合成能力而表现出更优的性能。耐酸微生物能够在极端酸性环境稳定增殖,是合成生物学研究领域极具潜力的新一代底盘细胞。基于耐酸微生物的新一代生物制造技术有望解决传统嗜中性模式菌发酵产酸所导致的产物抑制、杂菌污染、大量酸碱中和剂消耗以及盐类副产物污染等工业难题,可实现有机酸的清洁生产,成为可持续制造领域中一种颇具吸引力的绿色工艺。本文介绍了耐酸微生物底盘细胞的特征,阐释了耐酸机制以及提高微生物细胞酸耐受性的方法与手段。此外,本文还总结了适用于耐酸微生物的遗传元件、分子工具与操作策略,及其在有机酸等高价值生物基化学品合成领域的最新应用成果、面临的挑战与机遇,并展望了推动耐酸底盘细胞从实验室走向工业应用的方向,包括拓展遗传操作工具包为在耐酸底盘中进行多基因通路的理性重构与工业表型优化提供可操作手段、以及利用合成生物学与系统生物学手段提高耐酸底盘细胞工厂在工业场景中的适配能力。
Driven by advances in synthetic biology
microbial biomanufacturing
serving as a bridge between fundamental research and industrial applications
has demonstrated enormous potential in the large-scale production of a wide range of products
and has become a prominent research direction in the field of biomanufacturing. Compared with conventional microorganisms
extremophiles exhibit superior performance owing to their remarkable growth and biosynthetic capabilities under stressful conditions. Acid-tolerant microorganisms
capable of stable proliferation under extremely acidic conditions
represent a new generation of highly promising chassis cells in synthetic biology. Biomanufacturing based on acid-tolerant microorganisms
offering solutions to long-standing industrial challenges associated with traditional neutrophilic model strains
such as product inhibition caused by acid accumulation
microbial contamination
substantial consumption of acid–base neutralizing agents
and pollution from salt by-products
is expected to simplify biomanufacturing processes
enable the clean production of organic acids and open (non-sterile) continuous fermentation
and utilize low-cost substrates
thus emerging as an attractive green process for sustainable manufacturing. This review describes the characteristics of acid-tolerant microbial chassis
and subsequently elucidates the underlying acid tolerance mechanisms together with strategies for enhancing microbial acid resistance. In addition
we summarize the genetic elements
molecular tools
and operational strategies developed for acid-tolerant microorganisms
as well as recent advances in the application of acid-tolerant chassis cells in the biosynthesis of high-value bio-based chemicals such as organic acids. Finally
the challenges and future opportunities of acid-tolerant chassis cells are discussed. This review also outlines directions for advancing acid-tolerant chassis cells from the laboratory to industrial applications
which include expanding the genetic manipulation toolkit to provide operable means for the rational reconstruction of multi-gene pathways and the optimization of industrial phenotypes in acid-tolerant chassis
and leveraging synthetic biology and systems biology to improve the adaptability of acid-tolerant chassis cell factories in industrial settings. We hope that this review can provide theoretical guidance for the rational design and industrial application of acid-tolerant chassis cell factories.
2
Ye J W , Lin Y N , Yi X Q , et al . Synthetic biology of extremophiles: a new wave of biomanufacturing [J ] . Trends in Biotechnology , 2023 , 41 ( 3 ): 342 - 357 .
Zhang Z , Liu P , Zhang B , et al . De nov o biosynthesis of caffeic acid and chlorogenic acid in Escherichia coli via enzyme engineering and pathway engineering [J ] . ACS Synthetic Biology , 2025 , 14 ( 5 ): 1581 - 1593 .
Narayanan A K , Rao K R R , Megha K , et al . Toward sustainable production of bulnesol and elemol enabled by synthetic biology in Saccharomyces cerevisiae [J ] . Archives of Microbiology , 2026 , 208 ( 7 ): 332 .
Wang J , Ma W , Zhou J , et al . Microbial chassis design and engineering for production of ɤ-aminobutyric acid [J ] . World Journal of Microbiology and Biotechnology , 2024 , 40 ( 5 ): 159 .
Raj K , Venayak N , Mahadevan R . Novel two-stage processes for optimal chemical production in microbes [J ] . Metabolic Engineering , 2020 , 62 : 186 – 97 .
Kuang Z , Yan X , Yuan Y , et al . Advances in stress-tolerance elements for microbial cell factories [J ] . Synthetic and Systems Biotechnology , 2024 , 9 : 793 – 808 .
Gao S , Liao Y , He H , et al . Advance of tolerance engineering on microbes for industrial production [J ] . Synthetic and Systems Biotechnology , 2023 , 8 : 697 – 707 .
Tan S , Liu Z , Tran V G , et al . Issatchenkia orientalis as a platform organism for cost-effective production of organic acids [J ] . Metabolic Engineering , 2025 , 89 : 12 – 21 .
Gao X , Xu K , Ahmad N , et al . Recent advances in engineering of microbial cell factories for intelligent pH regulation and tolerance [J ] . Biotechnology Journal , 2021 , 16 ( 9 ): e2100151
Wang Y , Wang Y , Cui J , et al . Non-conventional yeasts: Promising cell factories for organic acid bioproduction [J ] . Trends in Biotechnology , 2025 , 43 ( 7 ): 1609 – 1626 .
Kraakman J R , Pok A , Smeulders M J , et al . Extremely acidophilic sulfur-oxidizing bacteria applied in biotechnological processes for gas purification [J ] . Journal of Environmental Science and Health, Part A , 2012 , 47 ( 7 ): 964 - 969 .
López-Martinez A , Martinez-Prado M A , Núñez-Ramirez D M , et al . Acidophilic bacteria for metal extraction: Biotechnological characteristics and applications [J ] . Brazilian Journal of Chemical Engineering , 2024 , 42 ( 1 ): 31 - 52 .
Zhang B , Li R , Yu L , et al L-lactic acid production via sustainable neutralizer-free route by engineering acid-tolerant yeast Pichia kudriavzevii [J ] . Journal of Agricultural and Food Chemistry , 2023 , 71 ( 29 ): 11131 - 11140 .
Zhong Y , Shang C , Tao H , et al . Boosting succinic acid production of Yarrowia lipolytica at low pH through enhancing product tolerance and glucose metabolism [J ] . Microbial Cell Factories , 2024 , 23 : 291 .
Avila-Cabre S , Perez-Trujillo M , Albiol J , et al . Engineering the synthetic β-alanine pathway in Komagataella phaffii for conversion of methanol into 3-hydroxypropionic acid [J ] . Microbial Cell Factories , 2023 , 22 ( 1 ): 237 .
Watcharawipas A , Runguphan W , Khamwachirapithak P , et al . Integrating yeast biodiversity and machine learning for predictive metabolic engineering [J ] . FEMS Yeast Research , 2025 , 25 : foaf072 .
Narayanan V , Sànchez i Nogué V , van Niel E W J , et al . Adaptation to low pH and lignocellulosic inhibitors resulting in ethanolic fermentation and growth of Saccharomyces cerevisiae [J ] . Amb Express , 2016 , 6 ( 1 ): 59 .
Li B , Liu N , Zhao X . Response mechanisms of Saccharomyces cerevisiae to the stress factors present in lignocellulose hydrolysate and strategies for constructing robust strains [J ] . Biotechnology for Biofuels , 2022 , 15 : 28 .
Nguyen T A , Kim H Y , Stocker S , et al . Pichia kudriavzevii ( Candida krusei ): a systematic review to inform the World Health Organisation priority list of fungal pathogens [J ] , Medical Mycology , 2024 , 62 ( 6 ): myad132 .
Tran K D , Le-Thi L , Vo H H , et al . Probiotic properties and safety evaluation in the invertebrate model host galleria mellonella of the Pichia kudriavzevii YGM091 strain isolated from fermented goat milk [J ] . Probiotics and Antimicrobial Proteins , 2024 , 16 : 1288 – 1303 .
Vijayakumar V E , Venkataraman K A . Systematic review of the potential of Pichia pastoris ( Komagataella phaffii ) as an alternative host for biologics production [J ] . Molecular Biotechnology , 2024 , 66 : 1621 – 1639 .
Zha J , Liu D , Ren J , et al . Advances in metabolic engineering of Pichia pastoris strains as powerful cell factories [J ] . Journal of Fungi , 2023 , 9 : 1027 .
Sae-Tang K , Bumrungtham P , Mhuantong W , et al . Engineering flocculation for improved tolerance and production of D-lactic acid in Pichia pastoris [J ] . Journal of Fungi , 2023 , 9 : 409 .
Guo F , Dai Z , Peng W , et al . Metabolic engineering of Pichia pastoris for malic acid production from methanol [J ] . Biotechnology and Bioengineering , 2021 , 118 : 357 – 371 .
Chen S , Zhang M , Wu X , et al . Promoter engineering for enhanced 3-hydroxypropionic acid production in Pichia pastoris [J ] . Synthetic and Systems Biotechnology , 2025 , 10 : 916 – 924 .
Smets J , Godoy H E , Goossenaerts J , et al ., Metabolic engineering and adaptive laboratory evolution of Kluyveromyces Marxianus for lactic acid production [J ] . Microbial Cell Factories , 2025 , 24 ( 1 ): 179 .
Perpetuini G , Tittarelli F , Perla C , et al . Influence of different aggregation states on volatile organic compounds released by dairy Kluyveromyces marxianus strains [J ] . Food Security , 2022 , 11 : 2910 .
Moon S Y , An N Y , Lee JY . Transforming non-conventional yeasts into key players in biotechnology: advances in synthetic biology applications [J ] . Frontiers in Microbiology , 2025 , 16 : 1600187 .
Mackenzie T , Raha P O , Lalit K , et al . Tri-Functional CRISPR screen reveals overexpression of QDR2 and QDR3 transporters increase fumaric acid production in Kluyveromyces marxianus [J ] . ACS Synthetic Biology , 2025 , 14 ( 12 ): 4907 – 4922 .
Lopes M , Miranda S M , Costa A R , et al . Yarrowia lipolytica as a biorefinery platform for effluents and solid wastes valorization—challenges and opportunities [J ] . Critical Reviews in Biotechnology , 2022 , 42 : 163 – 83 .
Fu J , Zaghen S , Lu H , et al . Reprogramming Yarrowia lipolytica metabolism for efficient synthesis of itaconic acid from flask to semipilot scale [J ] . Science Advances , 2024 , 10 : eadn0414 .
Pyne M E , Bagley J A , Narcross L , et al . Screening non-conventional yeasts for acid tolerance and engineering Pichia occidentalis for production of muconic acid [J ] . Nature Communications , 2023 , 14 ( 1 ): 5294 .
Wefelmeier K , Schmitz S , Haut A M . Engineering the methylotrophic yeast Ogataea polymorpha for lactate production from methanol [J ] . Frontiers in Bioengineering and Biotechnology , 2023 , 11 : 1223726 .
Park Y K , Nicaud J M , Ledesma-Amaro R . The engineering potential of Rhodosporidium toruloides as a workhorse for biotechnological applications [J ] . Trends in Biotechnology , 2018 , 36 ( 3 ): 304 – 317 .
Kuanyshev N , RAO C , BIEN B , et al . Domesticating a food spoilage yeast into an organic acid-tolerant metabolic engineering host: lactic acid production by engineered Zygosaccharomyces bailii [J ] . Biotechnology and Bioengineering , 2021 , 118 : 372 – 382 .
Mallick S , Das S . Acid-tolerant bacteria and prospects in industrial and environmental applications [J ] . Applied Microbiology and Biotechnology , 2023 , 107 ( 11 ): 3355 - 3374 .
Vardanyan A , Vyrides I . Acidophilic bioleaching at high dissolved organic compounds: Inhibition and strategies to counteract this [J ] . Minerals Engineering , 2019 , 143 : 105943 .
Xu Y , Li H , Zeng X C . A novel biofilm bioreactor derived from a consortium of acidophilic arsenite-oxidizing bacteria for the cleaning up of arsenite from acid mine drainage [J ] . Ecotoxicology , 2020 , 30 ( 7 ): 1437 - 1445 .
Zakalyukina Y V , Zenova G M . Antagonistic activity of soil acidophilic actinomycetes [J ] . Biology Bulletin , 2007 , 34 ( 4 ): 329 - 332 .
González E , Vera F , Scott F , et al . Acidophilic heterotrophs: Basic aspects and technological applications [J ] . Frontiers in Microbiology , 2024 , 15 : 1374800 .
Yan B , Chen H , Deng Z , et al . Functional analysis of a diterpene synthase for jiangxidienes and jiangxienols biosynthesis from Streptacidiphilus jiangxiensis [J ] . Chinese Chemical Letters , 2026 , 37 ( 9 ): 112790 .
Li Y , Yang J , Zhang X , et al . Integrative multi-omics identify key secondary metabolites linked to acid tolerance in Leptospirillum ferriphilum [J ] . Microorganisms , 2025 , 13 ( 11 ): 2493 .
Igbojionu L I , Martinez M M , Goddard A D , et al . Whole-cell biotechnological applications with thermoacidophilic Crenarchaeota: Opportunities and challenges [J ] . Process Biochemistry , 2026 , 160 : 86 - 101 .
Sakuntala M , Kim JR . Recent advances and challenges in the bioconversion of acetate to value-added chemicals [J ] . Bioresource Technology , 2022 , 364 : 128064 .
Guan N , Liu L , Shin H , et al . Systems-level understanding how Propionibacterium acidipropionici respond to propionic acid stress at the microenviron ment levels: mechanism and application [J ] . Journal of Biotechnology , 2013 , 167 : 56 – 63 .
Frousnoon T B , Pham N N , Wu Z Y , et al . Comparison of stress tolerance mechanisms between Saccharomyces cerevisiae and the multistress-tolerant Pichia kudriavzevii [J ] . FEMS Yeast Research , 2025 , 25 : foaf024 .
Rowbury RJ , Goodson M . PhoE porin of Escherichia coli and phosphate reversal of acid damage and killing and of acid induction of the CadA gene product [J ] . The Journal of Bacteriology , 1993 , 74 ( 6 ): 652 - 661 .
Jerez C A , Seeger M , Amaro A M . Phosphate starvation affects the synthesis of outer membrane proteins in Thiobacillus ferrooxidans [J ] , FEMS Microbiology Letters , 1992 , 98 ( 1-3 ): 29 – 33 .
Van de Vossenberg J L C M , Driessen A J M , Zillig W , et al . Bioenergetics and cytoplasmic membrane stability of the extremely acidophilic, thermophilic archaeon Picrophilus oshimae [J ] . Extremophiles , 1998 , 2 ( 2 ): 67 – 74 .
Peetermans A , Foulquié-Moreno M R , Thevelein J M . Mechanisms underlying lactic acid tolerance and its influence on lactic acid production in Saccharomyces cerevisiae [J ] . Microbial Cell , 2021 , 8 ( 6 ): 111 - 130 .
Xia X . Multiple regulatory mechanisms for pH homeostasis in the gastric pathogen, Helicobacter pylori [J ] . Advanced Genetics , 2022 , 109 : 39 - 69 .
Fütterer O , Angelov A , Liesegang H , et al . Genome sequence of Picrophilus torridus and its implications for life around pH 0 [J ] . Proceedings of the National Academy of Sciences , 2004 , 101 ( 24 ): 9091 - 9096
Auernik K S , Cooper C R , Kelly R M . Life in hot acid: Pathway analyses in extremely thermoacidophilic archaea [J ] . Current Opinion in Biotechnology , 2008 , 19 ( 5 ): 445 - 453 .
Golyshina O V , Tran H , Reva O N , et al . Metabolic and evolutionary patterns in the extremely acidophilic archaeon Ferroplasma acidiphilum Y T [J ] . Scientific Reports , 2017 , 7 ( 1 ): 3682 .
Seong Y J , Lee H J , Lee J E , et al . Physiological and metabolomic analysis of Issatchenkia orientalis MTY1 with multiple tolerance for cellulosic bioethanol production [J ] . Biotechnology Journal , 2017 , 12 ( 11 ): 1700110 .
Li Y , Wu Z , Li R , et al . Integrated transcriptomic and proteomic analysis of the acetic acid stress i n Issatchenkia orientalis [J ] . Journal of Food Biochemistry , 2020 , 44 ( 6 ): e13203 .
Ji H , Xu K , Dong X , et al . Transcriptional profiling reveals molecular basis and the role of arginine in response to low-pH stress in Pichia kudriavzevii [J ] . Journal of Bioscience and Bioengineering , 2020 , 130 ( 6 ): 588 – 595
Liu J , Guo T , Wang D , et al . Butanol production by a Clostridium beijerinckii mutant with high ferulic acid tolerance [J ] . Biotechnology and Applied Biochemistry , 2016 , 63 : 727 - 33 .
Fletcher E , Feizi A , Bisschops M M , et al . Evolutionary engineering reveals divergent paths when yeast is adapted to different acidic environments [J ] . Metabolic Engineering , 2017 , 39 : 19 - 28 .
Sun Y , Fukamachi T , Saito H , et al . Respiration and the F1Fo-ATPase enhance survival under acidic conditions in Escherichia coli [J ] . PLoS One , 2012 , 7 : e52577 .
Nygård Y , Mojzita D , Toivari M , et al . The diverse role of Pdr12 in resistance to weak organic acids [J ] . Yeast , 2014 , 31 : 219 – 232 .
Takabatake A , Kawazoe N , Izawa S . Plasma membrane proteins Yro2 and Mrh1 are required for acetic acid tolerance in Saccharomyces cerevisiae [J ] . Applied Microbiology and Biotechnology , 2015 , 99 : 2805 - 2814 .
Palma M , de Canaveira R F , Guerreiro J F , et al . Search for genes responsible for the remarkably high acetic acid tolerance of a Zygosaccharomyces bailii -derived interspecieshybrid strain [J ] . BMC Genomics , 2015 , 16 : 1070 .
Wu XC , Zhang LJ , Jin XN , et al . Deletion of JJJ1 improves acetic acid tolerance and bioethanol fermentation performance of Saccharomyces cerevisiae strains [J ] . Biotechnology Letters , 2016 , 38 : 1097 - 1106 .
Ding J , Holzwarth G , Bradford C S , et al . PEP3 overexpression shortens lag phase but does not alter growth rate in Saccharomyces cerevisiae exposed to acetic acid stress [J ] . Applied Microbiology and Biotechnology , 2015 , 99 : 8667 - 8680 .
Sandoval N R , Mills T Y , Zhang M , et al . Elucidating acetate tolerance in E. coli using a genome-wide approach [J ] . Metabolic Engineering , 2011 , 13 ( 2 ): 214 - 224 .
Garst A D , Bassalo M C , Pines G , et al ., Genome-wide mapping of mutations at single-nucleotide resolution for protein, metabolic and genome engineering [J ] . Nature Biotechnology , 2017 , 35 ( 1 ): 48 - 55 .
Xiao H , Shao Z , Jiang Y , et al . Exploiting Issatchenkia orientalis SD108 for succinic acid production [J ] . Microbial Cell Factory , 2014 , 13 : 1 - 11 .
Cao M , Fatma Z , Song X , et al . A genetic toolbox for metabolic engineering of Issatchenkia orientalis [J ] . Metabolic Engineering , 2020 , 59 : 87 - 97 .
Smith J R , Sislak C D , Mendoza P F , et al . Auxotrophy-independent plasmid shuttle vectors for applications in diverse yeasts [J ] . Applied Microbiology and Biotechnology , 2024 , 4 ( 1 ): 453 - 469 .
Tran V G , Cao M , Fatma Z , et al . Development of a CRISPR/Cas9- based tool for gene deletion in Issatchenkia orientalis [J ] . mSphere , 2019 , 4 ( 3 ): e00345 .
Sun W , Vila-Santa A , Liu N , et al . Metabolic engineering of an acid-tolerant yeast strain Pichia kudriavzevii for itaconic acid production [J ] . Metabolic Engineering Communications , 2020 , 10 : e00124
Wang Y , Gao S , Wu C , et al . Expanding the genetic toolkit of Pichia kudriavzevii E1 for enhanced mycoprotein production [J ] . Microbial Biotechnology , 2026 , 19 ( 9 ): e70447
Lee Y G , Kim C , Kuanyshev N , et al . Cas9-based metabolic engineering of Issatchenkia orientalis for enhanced utilization of cellulosic hydrolysates [J ] . Journal of Agricultural and Food Chemistry , 2022 , 70 ( 38 ): 12085 - 12094 .
Schwartz C M , Hussain MS , Blenner M , et al . Synthetic RNA polymerase iii promoters facilitate high-efficiency CRISPR-Cas9-mediated genome editing in Yarrowia lipolytica [J ] . ACS Synthetic Biology , 2016 , 5 : 356 - 359 .
Bae SJ , Park BG , Kim BG , et al . Multiplex gene disruption by targeted base editing of Yarrowia lipolytica genome using cytidine deaminase combined with the CRISPR/Cas9 system [J ] . Biotechnology Journal , 2020 , 15 : e1900238 .
Jiang W , Wang S , Ahlheit D , et al . High-throughput metabolic engineering of Yarrowia lipolytica through gene expression tuning [J ] . PNAS , 2025 , 122 ( 23 ): e2426686122 .
Larroude M , Rossignol T , Nicaud J M , et al . Synthetic biology tools for engineering Yarrowia lipolytica [J ] . Biotechnology Advances , 2018 , 36 : 2150 - 2164 .
Suthersa PF , Dinha HV , Fatma Z , et al ., Genome-scale metabolic reconstruction of the non-model yeast Issatchenkia orientalis SD108 and its application to organic acids production [J ] . Metabolic Engineering Communications , 2020 , 11 : e00148 .
Zhang S , Voigt C A . Engineered dcas9 with reduced toxicity in bacteria: Implications for genetic circuit design [J ] . Nucleic Acids Research , 2018 , 46 ( 20 ): 11115 - 11125 .
Arifah A Q , Vento J M , Kurrer I , et al . Cas9-independent tracrrna cytotoxicity in Lacticaseibacillus paracasei [J ] . microLife , 2025 , 6 : uqaf013 .
Xuan Q , Wang J , Nie Y , et al . Research progress and application of miniature CRISPR-Cas12 system in gene editing [J ] . International Journal of Molecular Sciences , 2024 , 25 ( 23 ): 12686 .
Meliawati M , Schilling C , Schmid J . Recent advances of Cas12a applications in bacteria [J ] . Applied Microbiology and Biotechnology , 2021 , 105 ( 8 ): 2981 - 2990 .
Zhang Y , Kubiak A M , Bailey T S , et al . Development of a CRISPR-Cas12a system for efficient genome engineering in clostridia [J ] . Microbiology Spectrum , 2023 , 11 ( 6 ): e02459-02423 .
Jung H , Inaba Y , Banta S . CRISPR/dCas12a knock-down of Acidithiobacillus ferrooxidans electron transport chain bc1 complexes enables enhanced metal sulfide bioleaching [J ] . Journal of Biological Chemistry , 2024 , 300 ( 9 ): 107703 .
Jung H , Inaba Y , West A C , et al . Overexpression of quorum sensing genes in Acidithiobacillus ferrooxidans enhances cell attachment and covellite bioleaching [J ] . Biotechnology Reports , 2023 , 38 : e00789 .
Kanao T . Tetrathionate hydrolase from the acidophilic microorganisms [J ] . Frontiers in Microbiology , 2024 , 15 : 1338669 .
Wang Z B , Li Y Q , Lin J Q , et al . The two-component system RSRS-RSRR regulates the tetrathionate intermediate pathway for thiosulfate oxidation in Acidithiobacillus caldus [J ] . Frontiers in Microbiology , 2016 , 7 : 1755 .
Moinier D , Byrne D , Amouric A , et al . The global redox responding RegB/RegA signal transduction system regulates the genes involved in ferrous iron and inorganic sulfur compound oxidation of the acidophilic Acidithiobacillus ferrooxidans [J ] . Frontiers in Microbiology , 2017 , 8 : 1277 .
Sepúlveda-Rebolledo P , González-Rosales C , Dopson M , et al . Comparative genomics of sigma factors in acidithiobacillia sheds light into the transcriptional regulatory networks involved in biogeochemical dynamics in extreme acidic environments [J ] . Microorganisms , 2025 , 13 ( 6 ): 1199 .
Berkner S , Wlodkowski A , Albers SV , et al . Inducible and constitutive promoters for genetic systems in Sulfolobus acidocaldarius [J ] . Extremophiles , 2010 , 14 ( 3 ): 249 - 259 .
Park H J , Bae J H , Ko H J , et al . Low-pH production of D-lactic acid using newly isolated acid tolerant yeast Pichia kudriavzevii NG7 [J ] . Biotechnology and Bioengineering , 2018 , 115 ( 9 ): 2232 - 2242 .
Wang Y , Cui J , Liu Z , et al . Industrial perspective validation of a neutralizer-free L-lactic acid production process by engineered acid-tolerant yeast Pichia kudriavzevii [J ] . Trends in Biotechnology , 2026 , 44 ( 6 ): 1660 - 1682 .
Lee YG , Kang NK , Kim C , et al . Self-buffering system for cost-effective production of lactic acid from glucose and xylose using acid-tolerant Issatchenkia orientalis [J ] . Bioresource Technology , 2024 , 399 : 130641 .
Altvater M , Tomico-Cuenca I , Mattanovich D , et al . Long-term adaptive laboratory evolution of Saccharomyces cerevisiae for high-titer lactic acid production at low pH [J ] . Microbial Cell Factories , 2025 , 24 ( 1 ): 242 .
Xi Y , Xu H , Zhan T , et al . Metabolic engineering of the acid-tolerant yeast Pichia kudriavzevii for efficient L-malic acid production at low pH [J ] , Metabolic Engineering , 2023 , 75 : 170 - 180 .
Wang Y , HAN Y , LIU H , et al . Engineering Yarrowia lipolytica to produce L-malic acid from glycerol [J ] . ACS Synthethic Biology , 2024 , 13 ( 11 ), 3635 - 3645 .
Tran V G , Mishra S , Bhagwat S S , et al . An end-to-end pipeline for succinic acid production at an industrially relevant scale using Issatchenkia orientalis [J ] . Nature Communications , 2023 , 14 ( 1 ): 6152 .
Tran V G , Tan S I , Xu H , et al . Decompartmentalization of the yeast mitochondrial metabolism to improve chemical production in Issatchenkia orientalis [J ] . Nature Communications , 2025 , 16 ( 1 ): 7110 .
Takayama S , Ozaki A , Konishi R , et al . Enhancing 3-hydroxypropionic acid production in combination with sugar supply engineering by cell surface-display and metabolic engineering of Schizosaccharomyces pombe [J ] . Microbial Cell Factories , 2018 , 17 : 176 .
Tan S I , Bhagwat S S , Martin T A , et al . High yield production of 3-hydroxypropionic acid using Issatchenkia orientalis [J ] . Nature Communications , 2026 , 117 ( 1 ): 899 .
Ning Y , Liu H , Zhang R , et al . Research progress on the construction of artificial pathways for the biosynthesis of adipic acid by engineered microbes [J ] . Fermentation , 2022 , 8 : 393 .
Ju J H , Oh B R , Heo S Y , et al . Production of adipic acid by short- and long chain fatty acid acyl-CoA oxidase engineered in yeast Candida tropicalis [J ] . Bioprocess and Biosystems Engineering , 2020 , 43 : 33 - 43 .
Ksiazek E . Citric acid: properties, microbial pro duction, and applications in industries [J ] . Molecules , 2024 , 29 : 22 .
Hesham A E , Mostafa Y S , AlSharqi L E O . Optimization of citric acid production by immobilized cells of novel yeast isolates [J ] . Mycobiology , 2020 , 48 : 122 - 132 .
Tapia J M , Muñoz J A , González F , et al . Mechanism of adsorption of ferric iron by extracellular polymeric substances (EPS) from a bacterium Acidiphilium sp [J ] . Water Science and Technology , 2011 , 64 : 1716 - 1722 .
González E , Zuleta C , Zamora G , et al . Production of poly (3-hydroxybutyrate) and extracellular polymeric substances from glycerol by the acidophile Acidiphilium cryptum [J ] . Extremophiles , 2023 , 27 : 3 .
Zolghadr B , Klingl A , Koerdt A , et al . Appendage-mediated surface adherence of Sulfolobus solfataricus [J ] . Journal of Bacteriology , 2010 , 192 : 104 - 110 .
Vidal P , Martínez-Martínez M , Fernandez-Lopez L , et al . Metagenomic mining for esterases in the microbial community of los rueldos acid mine drainage formation [J ] . Frontiers in Microbiology , 2022 , 13 : 868839 .
Golyshina O V , Golyshin P N , Timmis K N , et al . The 'pH optimum anomaly' of intracellular enzymes of Ferroplasma acidiphilum [J ] . Environmental Microbiology , 2006 , 8 : 416 - 425 .
Isobe K , Wakao N . Tween-hydrolyzing esterase produced by Acidiphilium sp [J ] . Journal of General and Applied Microbiology , 1996 , 42 : 285 - 295 .
Ade C , Marcelino T F , Dulchavsky M , et al . Microreactor equipped with naturally acid-resistant histidine ammonia lyase from an extremophile [J ] . Materials Advances , 2022 , 3 : 3649 - 3662 .
0
浏览量
0
下载量
0
CSCD
关联资源
相关文章
相关作者
相关机构
京公网安备11010102004073号