微生物法合成维生素E研究进展

张阳; 李志臻; 高馨语; 陈浩锋; 袁吉锋

doi:10.12211/2096-8280.2026-061

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微生物法合成维生素E研究进展

特约评述 | 更新时间：2026-10-26

- 微生物法合成维生素E研究进展
- Research Progress on Microbial Synthesis of Vitamin E
- 合成生物学 2026年页码：1-15
- 作者机构：
  
  1.厦门大学生命科学学院，细胞应激生物学国家重点实验室，福建厦门 361102
  2.厦门大学生物医药前沿交叉学院，福建厦门 361102
- 作者简介：
  
  张阳（1989—），男，副教授，硕士生导师。研究方向为紫色非硫光合细菌合成生物学，非模式微生物高效细胞工厂的构建；1C/2C、木质纤维素等非粮原料的高效生物转化。E-mail：y-zhang@xmu.edu.cn
  李志臻（2000—），女，博士研究生。研究方向为构建紫色非硫光合细菌的高效细胞工厂用于生产萜类化合物。E-mail：lizhizhen@stu.xmu.edu.cn
  袁吉锋（1984—），男，教授，博士生导师，闽江学者特聘教授，国家重点研发计划项目首席科学家。研究方向为构建高效细胞工厂用于生产各类高附加值产品（维生素、生物药、天然产物、化工品、蛋白制品等）；生物传感器用于菌株进化高通量筛选和生物检测；肠道益生菌的精准个性化治疗策略。E-mail：jfyuan@xmu.edu.cn
- 基金信息：
  
  国家重点研发计划(2024YFC3407000);国家自然科学基金(32270087;32400058);厦门市自然科学基金(3502Z202471013);中央高校基本科研业务费(20720240120;20720250011)
- DOI：10.12211/2096-8280.2026-061
  中图分类号： Q939.9
- 收稿：2026-07-13，
  
  修回：2026-09-29，
  
  网络首发：2026-10-26，
- 稿件说明：
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张阳，李志臻，高馨语，陈浩锋，袁吉锋. 微生物法合成维生素E研究进展［J］. 合成生物学， 2026， 7. DOI： 10.12211/2096-8280.2026-061

ZHANG Yang， LI Zhizhen， GAO Xinyu， CHEN Haofeng， YUAN Jifeng. Research Progress on Microbial Synthesis of Vitamin E［J］. Synthetic Biology Journal， 2026， 7. DOI： 10.12211/2096-8280.2026-061
张阳，李志臻，高馨语，陈浩锋，袁吉锋. 微生物法合成维生素E研究进展［J］. 合成生物学， 2026， 7. DOI： 10.12211/2096-8280.2026-061 DOI：

ZHANG Yang， LI Zhizhen， GAO Xinyu， CHEN Haofeng， YUAN Jifeng. Research Progress on Microbial Synthesis of Vitamin E［J］. Synthetic Biology Journal， 2026， 7. DOI： 10.12211/2096-8280.2026-061 DOI：

摘要

维生素E是一类脂溶性抗氧化剂，兼具重要的生理功能与庞大的市场需求。传统化学全合成法所得产物生物活性低、环境不友好；天然植物油提取法则受限于原料供应与成本，难以满足可持续发展需求。微生物发酵法凭借原料可再生、过程绿色、产物为单一天然构型等优势，成为维生素E绿色制造的前沿方向。本文重点阐述了大肠杆菌、酵母及紫色非硫光合细菌等工程化底盘细胞在维生素E异源合成中的研究进展，分析了前体供应强化、关键酶优化、膜工程与转运工程、辅因子优化及发酵工艺改进等策略。近年来，合成生物学的发展为微生物异源合成维生素E提供了工程化改造的新思路。通过酶工程改造、转运蛋白筛选及原位萃取等技术突破，结合传统的代谢工程改造，维生素E产量已从微克每升级别提升至克级每升水平，其中球形红细菌中生育酚产量达到3.51 g/L，创下最高纪录。最后，本文从新型酶资源挖掘、辅因子全局优化、产物耐受性改造、发酵—分离耦合、人工智能驱动的细胞工厂设计等方面展望了未来的研究方向，以期为维生素E及其他脂溶性天然产物的微生物合成提供参考。

Abstract

Vitamin E is a group of essential lipid-soluble vitamins with antioxidant

anti-aging

anti-tumor and other physiological activities. It plays critical roles in maintaining normal human physiological functions

preventing cardiovascular diseases

and treating Alzheimer's disease. As living standards improve globally

vitamin E is widely utilized as a nutritional supplement and antioxidant in feed

pharmaceutical

food and cosmetic industries

generating enormous market demand. At present

more than 80% of vitamin E worldwide is manufactured via total chemical synthesis. However

traditional chemical synthesis relies on petroleum-based raw materials

causes severe environmental pollution

and fails to meet the market demand for vitamin E monomers with specific stereoconfigurations. Extraction from natural vegetable oils is constrained by raw material supply and high production costs

and cannot satisfy sustainable development requirements. By comparison

microbial fermentation has emerged as a sustainable alternative for biomanufacturing of vitamin E

owing to advantages such as renewable feedstocks

eco-friendly manufacturing processes

and the production of natural single stereoisomers. This review systematically summarizes research advances in the heterologous biosynthesis of vitamin E using engineered chassis cells including

Escherichia coli

yeast and purple non-sulfur photosynthetic bacteria. Engineering strategies—including enhancement of precursor supply

optimization

of key enzymes

membrane and transport engineering

cofactor tuning

as well as fermentation process upgrading—are critically analyzed. In recent years

advances in synthetic biology have offered innovative strategies for engineering microbial hosts to heterologously synthesize vitamin E. Combined with traditional metabolic engineering

technological breakthroughs such as enzyme engineering

transporter screening

and in situ extraction have drastically raised vitamin E titers from micrograms per liter to grams per liter levels. Notably

the record-high tocopherol titer of 3.51 g/L has been achieved in engineered

Rhodobacter sphaeroides

. Finally

future research perspectives are proposed from five aspects: exploration of novel enzyme resources

global cofactor optimization

strain engineering for improved product tolerance

integrated fermentation-separation systems

and artificial intelligence-assisted design of microbial cell factories. This work aims to provide theoretical and technical references for the microbial synthesis of vitamin E and other lipid-soluble natural products.

关键词

Keywords

references

FRYER M J . The antioxidant effects of thylakoid vitamin E (α-tocopherol) [J ] . Plant , Cell & Environment, 1992 , 15 ( 4 ): 381 - 392 .

MATRINGE M , KSAS B , REY P , et al . Tocotrienols, the unsaturated forms of vitamin E, can function as antioxidants and lipid protectors in tobacco leaves [J ] . Plant Physiology , 2008 , 147 ( 2 ): 764 - 778 .

JOMOVA K , RAPTOVA R , ALOMAR S Y , et al . Reactive oxygen species, toxicity, oxidative stress, and antioxidants: chronic diseases and aging [J ] . Archives of Toxicology , 2023 , 97 ( 10 ): 2499 - 2574 .

BOREL P , PREVERAUD D , DESMARCHELIER C . Bioavailability of vitamin E in humans: an update [J ] . Nutrition Reviews , 2013 , 71 ( 6 ): 319 - 331 .

JIANG Q . Metabolism of natural forms of vitamin E and biological actions of vitamin E metabolites [J ] . Free Radical Biology and Medicine , 2022 , 179 : 375 - 387 .

DELLAPENNA D , POGSON B J . VITAMIN SYNTHESIS IN PLANTS: Tocopherols and carotenoids [J ] . Annual Review of Plant Biology , 2006 , 57 : 711 - 738 .

MÈNE-SAFFRANÉ L . Vitamin E biosynthesis and its regulation in plants [J ] . Antioxidants , 2018 , 7 ( 1 ): 2 .

TRABER M G , ATKINSON J . Vitamin E, antioxidant and nothing more [J ] . Free Radical Biology and Medicine , 2007 , 43 ( 1 ): 4 - 15 .

BURTON G W , TRABER M G , ACUFF R V , et al . Human plasma and tissue alpha-tocopherol concentrations in response to supplementation with deuterated natural and synthetic vitamin E [J ] . The American Journal of Clinical Nutrition , 1998 , 67 ( 4 ): 669 - 684 .

KAMAL-ELDIN A , APPELQVIST L-Å . The chemistry and antioxidant properties of tocopherols and tocotrienols [J ] . Lipids , 1996 , 31 ( 7 ): 671 - 701 .

SALIMATH S S , ROMSDAHL T B , KONDA A R , et al . Production of tocotrienols in seeds of cotton ( Gossypium hirsutum L.) enhances oxidative stability and offers nutraceutical potential [J ] . Plant Biotechnology Journal , 2021 , 19 ( 6 ): 1268 - 1282 .

YANG J , YU H , YE L . Evolution of vitamin E production: From chemical synthesis and plant extraction to microbial cell factories [J ] . Journal of Agricultural and Food Chemistry , 2024 , 72 ( 50 ): 27701 - 27714 .

ZHANG R , REN Y , ZHAO Y , et al . A century of vitamin E research: The innovative journey from basic biology to synthetic bio-manufacturing [J ] . Journal of Integrative Plant Biology , 2026 , 68 ( 4 ): 985 - 1012 .

ALBERMANN C , GHANEGAONKAR S , LEMUTH K , et al . Biosynthesis of the vitamin E compound δ-tocotrienol in recombinant Escherichia coli cells [J ] . ChemBioChem , 2008 , 9 ( 15 ): 2524 - 2533 .

GHANEGAONKAR S , CONRAD J , BEIFUSS U , et al . Towards the in vivo production of tocotrienol compounds: Engineering of a plasmid-free Escherichia coli strain for the heterologous synthesis of 2-methyl-6-geranylgeranyl-benzoquinol [J ] . Journal of Biotechnology , 2013 , 164 ( 2 ): 238 - 247 .

SUN H , YANG J , LIN X , et al . De novo high-titer production of delta-tocotrienol in recombinant Saccharomyces cerevisiae [J ] . Journal of Agricultural and Food Chemistry , 2020 , 68 ( 29 ): 7710 - 7717 .

SHEN B , ZHOU P , JIAO X , et al . Fermentative production of vitamin E tocotrienols in Saccharomyces cerevisiae under cold-shock-triggered temperature control [J ] . Nature Communications , 2020 , 11 ( 1 ): 5155 .

JIAO X , SHEN B , LI M , et al . Secretory production of tocotrienols in Saccharomyces cerevisiae [J ] . ACS Synthetic Biology , 2022 , 11 ( 2 ): 788 - 799 .

HAN L , WU Y , XU Y , et al . Engineered Saccharomyces cerevisiae for de novo δ-tocotrienol biosynthesis [J ] . Systems Microbiology and Biomanufacturing , 2024 , 4 ( 1 ): 150 - 164 .

JIAO X , BIAN Q , FENG T , et al . Efficient secretory production of δ-tocotrienol by combining pathway modularization and transportation engineering [J ] . Journal of Agricultural and Food Chemistry , 2023 , 71 ( 23 ): 9020 - 9030 .

XIANG J , LIU M , WANG X , et al . Combined metabolic and enzymatic engineering for de novo biosynthesis of δ-tocotrienol in Yarrowia lipolytica [J ] . Synthetic and Systems Biotechnology , 2025 , 10 ( 3 ): 719 - 727 .

XU C , JIAO X , ZHAO Q , et al . Membrane transporter mining and enzyme engineering drive high-titer δ-tocotrienol production in Yarrowia lipolytica [J ] . Biochemical Engineering Journal , 2026 , 228 : 110079 .

ZHANG Y , LI Z , CAO L , et al . A nonclassical biosynthetic route in bacteria towards tocopherol synthesis [J ] . Nature Metabolism , 2026 , doi: 10.1038/s42255-026-01576-y http://dx.doi.org/10.1038/s42255-026-01576-y .

SAVIDGE B , WEISS J D , WONG Y H H , et al . Isolation and characterization of homogentisate phytyltransferase genes from Synechocystis sp. PCC 6803 and Arabidopsis [J ] . Plant Physiology , 2002 , 129 ( 1 ): 321 - 332 .

COLLAKOVA E , DELLAPENNA D . Homogentisate phytyltransferase activity is limiting for tocopherol biosynthesis in Arabidopsis [J ] . Plant Physiology , 2003 , 131 ( 2 ): 632 - 642 .

COLLAKOVA E , DELLAPENNA D . The role of homogentisate phytyltransferase and other tocopherol pathway enzymes in the regulation of tocopherol synthesis during abiotic stress [J ] . Plant Physiology , 2003 , 133 ( 2 ): 930 - 940 .

CAHOON E B , HALL S E , RIPP K G , et al . Metabolic redesign of vitamin E biosynthesis in plants for tocotrienol production and increased antioxidant content [J ] . Nature Biotechnology , 2003 , 21 ( 9 ): 1082 - 1087 .

KELLER Y , BOUVIER F , D'HARLINGUE A , CAMARA B . Metabolic compartmentation of plastid prenyllipid biosynthesis [J ] . European Journal of Biochemistry , 1998 , 251 ( 1-2 ): 413 - 417 .

HORVATH G , WESSJOHANN L , BIGIRIMANA J , et al . Differential distribution of tocopherols and tocotrienols in photosynthetic and non-photosynthetic tissues [J ] . Phytochemistry , 2006 , 67 ( 12 ): 1185 - 1195 .

COLLAKOVA E , DELLAPENNA D . Isolation and functional analysis of homogentisate phytyltransferase from Synechocystis sp. PCC 6803 and Arabidopsis [J ] . Plant Physiology , 2001 , 127 ( 3 ): 1113 - 1124 .

SATTLER S E , CAHOON E B , COUGHLAN S J , et al . Characterization of tocopherol cyclases from higher plants and cyanobacteria: Evolutionary implications for tocopherol synthesis and function [J ] . Plant Physiology , 2003 , 132 ( 4 ): 2184 - 2195 .

SHINTANI D , DELLAPENNA D . Elevating the vitamin E content of plants through metabolic engineering [J ] . Science , 1998 , 282 ( 5396 ): 2098 - 2100 .

ZHANG C , CAHOON R E , HUNTER S C , et al . Genetic and biochemical basis for alternative routes of tocotrienol biosynthesis for enhanced vitamin E antioxidant production [J ] . The Plant Journal , 2013 , 73 ( 4 ): 628 - 639 .

KEASLING J D . Manufacturing molecules through metabolic engineering [J ] . Science , 2010 , 330 ( 6009 ): 1355 - 1358 . [35 ] NIELSEN J, KEASLING J D. Engineering cellular metabolism [J ] . Cell, 2016 , 164 ( 6 ): 1185 - 1197 .

QI Q G , HAO M , NG W O , et al . Application of the Synechococcus nirA promoter to establish an inducible expression system for engineering the Synechocystis tocopherol pathway [J ] . Applied and Environmental Microbiology , 2005 , 71 ( 10 ): 5678 - 5684 .

王岩岩 , 刘林霞 , 金朝霞 , 等 . 代谢工程在维生素生产中的应用及研究进展 [J ] . 生物工程学报 , 2021 , 37 ( 5 ): 1748 - 1770 .

WANG Y Y , LIU L X , JIN Z X , et al . Advances in metabolic engineering for vitamins production [J ] . Chinese Journal of Biotechnology , 2021 , 37 ( 5 ): 1748 - 1770 .

PONTRELLI S , CHIU T Y , LAN E I , et al . Escherichia coli as a host for metabolic engineering [J ] . Metabolic Engineering , 2018 , 50 : 16 - 46 .

RINALDI M A , FERRAZ C A , SCRUTTON N S . Alternative metabolic pathways and strategies to high-titre terpenoid production in Escherichia coli [J ] . Natural Product Reports , 2022 , 39 ( 1 ): 90 - 118 .

薛小莉 , 覃重军 . 大肠杆菌最小基因组分析和删减进展 [J ] . 生命科学 , 2013 , 25 ( 10 ): 978 - 982 .

XUE X L , QIN Z J . Progresses in the analysis and development of Escherichia coli minimal genome [J ] . Chinese Journal of Life Science , 2013 , 25 ( 10 ): 978 - 982 .

GAO H , QIU Z , WANG X , et al . Recent advances in genome-scale engineering in Escherichia coli and their applications [J ] . Engineering Microbiology , 2024 , 4 ( 1 ): 100115 .

JIANG M , ZHANG H . Engineering the shikimate pathway for biosynthesis of molecules with pharmaceutical activities in E. coli [J ] . Current Opinion in Biotechnology , 2016 , 42 : 1 - 6 .

XU W , YUAN J , YANG S , et al . Programming saposin-mediated compensatory metabolic sinks for enhancing ubiquinone productions [J ] . ACS Synthetic Biology , 2016 , 5 ( 12 ): 1404 - 1411 .

CLUIS C P , EKINS A , NARCROSS L , et al . Identification of bottlenecks in Escherichia coli engineered for the production of CoQ10 [J ] . Metabolic Engineering , 2011 , 13 ( 6 ): 733 - 744 .

LU Z , HUANG Z , WU Z , et al . High-level production of vitamin K2 in Escherichia coli via modular molecular engineering [J ] . Synthetic and Systems Biotechnology , 2026 , 12 : 42 - 51 .

LI Z , XU K , CHEN Y , et al . Rational design of heptaprenyl diphosphate synthase and metabolic optimization drive biosynthesis of menaquinone-7 by Escherichia coli [J ] . Bioresource Technology , 2026 , 135786 .

UBEROS H , AUGUSTIN M , LIÉBANA A , et al . Comparative study of the influence of melatonin and vitamin E on the surface characteristics of Escherichia coli [J ] . Letters in Applied Microbiology , 2001 , 32 ( 5 ): 303 - 306 .

ORSI E , BEEKWILDER J , EGGINK G , et al . The transition of Rhodobacter sphaeroides into a microbial cell factory [J ] . Biotechnology and Bioengineering , 2021 , 118 ( 2 ): 531 - 541 .

WANG J , KANG Q , CHEN X , et al . Systematic reprogramming of Rhodobacter sphaeroides for efficient biosynthesis of Coenzyme Q 10 and porphyrins [J ] . ACS Synthetic Biology , 2026 , 15 ( 6 ): 2196 - 2208 .

HE X , WANG D , LIU J , et al . Engineering the methylerythritol phosphate pathway and using a temporal promoter for enhanced lycopene production in Rhodobacter sphaeroides HY01 [J ] . Journal of Agricultural and Food Chemistry , 2024 , 72 ( 50 ): 28040 - 28047 .

ORSI E , BEEKWILDER J , VAN GELDER D , et al . Functional replacement of isoprenoid pathways in Rhodobacter sphaeroides [J ] . Microbial Biotechnology , 2020 , 13 ( 4 ): 1082 - 1093 .

CHEN H , WANG Y , WANG W , et al . High-yield porphyrin production through metabolic engineering and biocatalysis [J ] . Nature Biotechnology , 2025 , 43 ( 10 ): 1717 - 1727 .

ZHANG Y , SONG X , LAI Y , MO Q , YUAN J . High-yielding terpene-based biofuel production in Rhodobacter capsulatus [J ] . ACS Synthetic Biology , 2021 , 10 ( 6 ): 1545 - 1552 .

MOUGIAKOS I , ORSI E , GHIFFARY M R , et al . Efficient Cas9-based genome editing of Rhodobacter sphaeroides for metabolic engineering [J ] . Microbial Cell Factories , 2019 , 18 ( 1 ): 204 .

KOSTANJŠEK M , RAYNAL A , DIMOPOULOS G , et al . Rhodo-Box: A synthetic biology toolbox to facilitate metabolic engineering of Rhodobacter sphaeroides [J ] . ACS Synthetic Biology , 2026 , 15 ( 4 ): 1400 - 1411 .

BOTSTEIN D , FINK G R . Yeast: an experimental organism for 21st century biology [J ] . Genetics , 2011 , 189 ( 3 ): 695 - 704 .

江丽红 , 董昌 , 黄磊 , 等 . 酿酒酵母代谢工程技术 [J ] . 生物工程学报 , 2021 , 37 ( 5 ): 1578 - 1602 .

JIANG L H , DONG C , HUANG L , et al . Metabolic engineering tools for Saccharomyces cerevisiae [J ] . Chinese Journal of Biotechnology , 2021 , 37 ( 5 ): 1578 - 1602 .

陈明凯 , 叶丽丹 , 于洪巍 . 代谢改造酿酒酵母合成萜类化合物的研究进展 [J ] . 生物工程学报 , 2021 , 37 ( 6 ): 2085 - 2104 .

CHEN M K , YE L D , YU H W . Advances in metabolic engineering of Saccharomyces cerevisiae for terpenoids biosynthesis [J ] . Chinese Journal of Biotechnology , 2021 , 37 ( 6 ): 2085 - 2104 .

ZHANG Q , YU S , LYU Y , et al . Systematically engineered fatty acid catabolite pathway for the production of (2 S )-naringenin in Saccharomyces cerevisiae [J ] . ACS Synthetic Biology , 2021 , 10 ( 5 ): 1166 - 1175 .

SRINIVASAN P , SMOLKE C D . Biosynthesis of medicinal tropane alkaloids in yeast [J ] . Nature , 2020 , 585 ( 7826 ): 614 - 619 .

LIAN J , MISHRA S , ZHAO H . Recent advances in metabolic engineering of Saccharomyces cerevisiae : New tools and their applications [J ] . Metabolic Engineering , 2018 , 50 : 85 - 108 .

栾韬 , 尹梦琦 , 王明 , 康秀龙 , 赵建志 , 鲍晓明 . 酿酒酵母细胞器区室化合成化学品的研究进展 [J ] . 生物工程学报 , 2023 , 39 ( 6 ): 2334 - 2358 .

LUAN T , YIN M Q , WANG M , KANG X L , ZHAO J Z , BAO X M . Advances in the production of chemicals by organelle compartmentalization in Saccharomyces cerevisiae [J ] . Chinese Journal of Biotechnology , 2023 , 39 ( 6 ): 2334 - 2358 .

刘世昌 , 高教琪 , 周雍进 . 常见酵母的代谢工程研究进展 [J ] . 生物工程学报 , 2025 , 41 ( 11 ): 4037 - 4051 .

LIU S C , GAO J Q , ZHOU Y J . Advances in metabolic engineering of common yeast species [J ] . Chinese Journal of Biotechnology , 2025 , 41 ( 11 ): 4037 - 4051 .

FAN C , FAN J , CHEN H , et al . Switching the yeast metabolism via modulation of sugar phosphorylation [J ] . Metabolic Engineering , 2025 , 89 : 76 - 85 .

WANG Z , QI X , REN X , et al . Synthetic evolution of Saccharomyces cerevisiae for biomanufacturing: Approaches and applications [J ] . mLife , 2025 , 4 ( 1 ): 1 - 16 .

LEDESMA-AMARO R , NICAUD J M . Yarrowia lipolytica as a biotechnological chassis to produce usual and unusual fatty acids [J ] . Progress in Lipid Research , 2016 , 61 : 40 - 50 .

ABDEL-MAWGOUD A M , MARKHAM K A , PALMER C M , et al . Metabolic engineering in the host Yarrowia lipolytica [J ] . Metabolic Engineering , 2018 , 50 : 192 - 208 .

XU P , QIAO K , AHN W S , et al . Engineering Yarrowia lipolytica as a platform for synthesis of drop-in transportation fuels and oleochemicals [J ] . Proceedings of the National Academy of Sciences of the United States of America , 2016 , 113 ( 39 ): 10848 - 10853 .

NING Y , LIU M , RU Z , et al . Efficient synthesis of squalene by cytoplasmic-peroxisomal engineering and regulating lipid metabolism in Yarrowia lipolytica [J ] . Bioresource Technology , 2024 , 395 : 130379 .

LIU S C , XU L , SUN Y , et al . Sustainable high-titer production of β-elemene in Yarrowia lipolytica via modular engineering, pathway refactoring, and a chimeric germacrene A synthase [J ] . Chemical Engineering Journal , 2026 , 528 : 172276 .

SHANG Y , ZHANG P , WEI W , et al . Metabolic engineering for the high-yield production of polydatin in Yarrowia lipolytica [J ] . Bioresource Technology , 2023 , 381 : 129129 .

LIU H-H , JI X J , HUANG H . Biotechnological applications of Yarrowia lipolytica : Past, present and future [J ] . Biotechnology Advances , 2015 , 33 ( 8 ): 1522 - 1546 .

MILLER K K , ALPER H S . Yarrowia lipolytica : more than an oleaginous workhorse [J ] . Applied Microbiology and Biotechnology , 2019 , 103 ( 23 ): 9251 - 9262 .

KIKUCHI Y , TSUJIMOTO K , KURAHASHI O . Mutational analysis of the feedback sites of phenyl alanine-sensitive 3-deoxy-D-arabino-heptulosonate-7-phosphate synthase of Escherichia coli [J ] . Applied and Environmental Microbiology , 1997 , 63 ( 2 ): 761 - 762 .

LÜTKE EVERSLOH T , STEPHANOPOULOS G . L -Tyrosine production by deregulated strains of Escherichia coli [J ] . Applied Microbiology and Biotechnology , 2007 , 75 ( 1 ): 103 - 110 .

GOLD N D , GOWEN C M , LUSSIER F X , et al . Metabolic engineering of a tyrosine-overproducing yeast platform using targeted metabolomics [J ] . Microbial Cell Factories , 2015 , 14 ( 1 ): 73 .

XU S , WANG Q , ZENG W , et al . Construction of a heat-inducible Escherichia coli strain for efficient de novo biosynthesis of L -tyrosine [J ] . Process Biochemistry , 2020 , 92 : 85 - 92 .

PING J , WANG L , QIN Z , ZHOU Z , ZHOU J . Synergetic engineering of Escherichia coli for efficient production of L -tyrosine [J ] . Synthetic and Systems Biotechnology , 2023 , 8 ( 4 ): 724 - 731 .

KOMA D , KISHIDA T , YOSHIDA E , et al . Chromosome engineering to generate plasmid-free phenylalanine- and tyrosine-overproducing Escherichia coli strains that can be applied in the generation of aromatic-compound-producing bacteria [J ] . Applied and Environmental Microbiology , 2020 , 86 ( 14 ).

BONGERS M , CHRYSANTHOPOULOS P K , BEHRENDORFF J B Y H , et al . Systems analysis of methylerythritol-phosphate pathway flux in E. coli : Insights into the role of oxidative stress and the validity of lycopene as an isoprenoid reporter metabolite [J ] . Microbial Cell Factories , 2015 , 14 ( 1 ): 193 .

XIE W , LV X , YE L , ZHOU P , YU H . Construction of lycopene-overproducing Saccharomyces cerevisiae by combining directed evolution and metabolic engineering [J ] . Metabolic Engineering , 2015 , 30 : 69 - 78 .

朱航志 , 蒋珊 , 陈丹 , 等 . 引入新型异戊二烯醇利用途径促进解脂耶氏酵母中β-胡萝卜素的合成 [J ] . 中国生物工程杂志 , 2021 , 41 ( 4 ): 37 - 46 .

ZHU H Z , JIANG S , CHEN D , et al . Improving the biosynthesis of β-carotene in yarrowia lipolytica by introducing an artificial isopentenol utilization pathway [J ] . China Biotechnology , 2021 , 41 ( 4 ): 37 - 46 .

KOCH M , LEMKE R , HEISE K P , MOCK H P . Characterization of γ-tocopherol methyltransferases from Capsicum annuum L and Arabidopsis thaliana [J ] . European Journal of Biochemistry , 2003 , 270 ( 1 ): 84 - 92 .

TEWARI K , DAHUJA A , SACHDEV A , et al . Molecular cloning, heterologous expression and functional characterization of gamma tocopherol methyl transferase (γ-TMT) from Glycine max [J ] . Protein Expression and Purification , 2017 , 140 : 81 - 89 .

TAVVA V S , KIM Y H , KAGAN I A , et al . Increased α-tocopherol content in soybean seed overexpressing the Perilla frutescens γ-tocopherol methyltransferase gene [J ] . Plant Cell Reports , 2007 , 26 ( 1 ): 61 - 70 .

ZHANG L , LUO Y , LIU B , et al . Overexpression of the maize γ-tocopherol methyltransferase gene (ZmTMT) increases α-tocopherol content in transgenic Arabidopsis and maize seeds [J ] . Transgenic Research , 2020 , 29 ( 1 ): 95 - 104 .

SOMSRI A , CHU S H , NAWADE B , et al . Harnessing γ-TMT genetic variations and haplotypes for vitamin E diversity in the korean rice collection [J ] . Antioxidants , 2024 , 13 : 234 .

CHEN H , ZHENG F , FENG X , et al . Engineering archaeal membrane-spanning lipid GDGT biosynthesis in bacteria: Implications for early life membrane transformations [J ] . mLife , 2025 , 4 ( 2 ): 193 - 204 .

ADDLEE H A , HUNTER C N . Physical mapping and functional assignment of the geranylgeranyl-bacteriochlorophyll reductase gene, bchP, of Rhodobacter sphaeroides [J ] . Journal of Bacteriology , 1999 , 181 ( 23 ): 7248 - 7255 .

WALTHER T C , FARESE R V . Lipid droplets and cellular lipid metabolism [J ] . Annual Review of Biochemistry , 2012 , 81 : 687 - 714 .

BU X , LIN J Y , CHENG J , et al . Engineering endogenous ABC transporter with improving ATP supply and membrane flexibility enhances the secretion of β-carotene in Saccharomyces cerevisiae [J ] . Biotechnology for Biofuels , 2020 , 13 ( 1 ): 168 .

VERWAAL R , JIANG Y , WANG J , et al . Heterologous carotenoid production in Saccharomyces cerevisiae induces the pleiotropic drug resistance stress response [J ] . Yeast , 2010 , 27 ( 12 ): 983 - 998 .

MORAN G R . 4-Hydroxyphenylpyruvate dioxygenase [J ] . Archives of Biochemistry and Biophysics , 2005 , 433 ( 1 ): 117 - 128 .

MURAKAMI M , SHIBUYA K , NAKAYAMA T , et al . Geranylgeranyl reductase involved in the biosynthesis of archaeal membrane lipids in the hyperthermophilic archaeon Archaeoglobus fulgidus [J ] . The FEBS Journal , 2007 , 274 ( 3 ): 805 - 814 .

CUI G Z , MA Z , LIU Y J , et al . Overexpression of glucose-6-phosphate dehydrogenase enhanced the polyunsaturated fatty acid composition of Aurantiochytrium sp. SD116 [J ] . Algal Research , 2016 , 19 : 138 - 145 .

YUAN L , QIN Y L , ZOU Z C , et al . Enhancing intracellular NADPH bioavailability through improving pentose phosphate pathway flux and its application in biocatalysis asymmetric reduction reaction [J ] . Journal of Bioscience and Bioengineering , 2022 , 134 ( 6 ): 528 - 533 .

ZHAN M , KAN B , DONG J , et al . Metabolic engineering of Corynebacterium glutamicum for improved L -arginine synthesis by enhancing NADPH supply [J ] . Journal of Industrial Microbiology and Biotechnology , 2019 , 46 ( 1 ): 45 - 54 .

ZHANG Y , ZHOU Z , WU J , et al . Construction and application of an efficient methylation platform in Escherichia coli based on SAM regeneration [J ] . Chemical Engineering Journal , 2025 , 512 : 162642 .

MONTAÑO LÓPEZ J , DURAN L , AVALOS J L . Physiological limitations and opportunities in microbial metabolic engineering [J ] . Nature Reviews Microbiology , 2022 , 20 ( 1 ): 35 - 48 .

LIU L , DING D , WANG H , et al . Balancing cell growth and product synthesis for efficient microbial cell factories [J ] . Advanced Science , 2025 , 12 ( 40 ): e2510649 .

DIANAT M , STRAATEN S , MARITATO A , et al . Exploration of in situ extraction for enhanced triterpenoid production by Saccharomyces cerevisiae [J ] . Microbial Biotechnology , 2024 , 17 ( 12 ): e70061 .

COMBES J , IMATOUKENE N , COUVREUR J , et al . Intensification of p -coumaric acid heterologous production using extractive biphasic fermentation [J ] . Bioresource Technology , 2021 , 337 : 125436 .

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