从刺激响应到智能调控：磁场驱动的合成细胞调控

姚宇航; 陈兆委

doi:10.12211/2096-8280.2026-057

您当前的位置：

首页 >

文章列表页 >

从刺激响应到智能调控：磁场驱动的合成细胞调控

评论 | 更新时间：2026-09-01

- 从刺激响应到智能调控：磁场驱动的合成细胞调控
- From stimuli-responsiveness to intelligent regulation： magnetic field-driven regulation of synthetic cells
- 合成生物学 2026年页码：1-12
- 作者机构：
  
  环境分析与检测研究所，福州大学化学学院，福建　福州　350108
- 作者简介：
  
  姚宇航（1992—），男，副教授，博士。研究方向为仿生人工细胞、生物正交催化及人工金属酶等。 E-mail：yuhangyao@fzu.edu.cn
  陈兆委（1988—），男，教授，博士，博士生导师。研究方向为仿生人工细胞、生物正交催化及生物医学工程等。发表SCI论文120余篇，获得海外高层次人才等项目。担任基金和人才项目评审专家、《Nano Research》等期刊青年编委。 E-mail：chenzw@fzu.edu.cn
- 基金信息：
  
  国家自然科学基金(22277011;22577014;22404025;22421002)
- DOI：10.12211/2096-8280.2026-057
  中图分类号： Q939.9;Q789
- 收稿：2026-06-22，
  
  修回：2026-08-26，
  
  网络首发：2026-09-01，
- 稿件说明：
移动端阅览
姚宇航，陈兆委. 从刺激响应到智能调控：磁场驱动的合成细胞调控［J］. 合成生物学， 2026， 7. DOI： 10.12211/2096-8280.2026-057

YAO Yuhang， CHEN Zhaowei. From stimuli-responsiveness to intelligent regulation： magnetic field-driven regulation of synthetic cells［J］. Synthetic Biology Journal， 2026， 7. DOI： 10.12211/2096-8280.2026-057
姚宇航，陈兆委. 从刺激响应到智能调控：磁场驱动的合成细胞调控［J］. 合成生物学， 2026， 7. DOI： 10.12211/2096-8280.2026-057 DOI：

YAO Yuhang， CHEN Zhaowei. From stimuli-responsiveness to intelligent regulation： magnetic field-driven regulation of synthetic cells［J］. Synthetic Biology Journal， 2026， 7. DOI： 10.12211/2096-8280.2026-057 DOI：

摘要

合成细胞是指通过生物工程技术，利用DNA、蛋白质和脂质等基本分子组件“自下而上”人工构建的、能够执行类似天然生命功能的仿生系统，正成为研究生命起源与开发智能诊疗器件的前沿平台。然而，如何以非侵入、深穿透的外部刺激实现远程、时空精准的功能调控，始终是将这类系统推向实际应用的关键瓶颈。近期，牛津大学Michael J. Booth课题组在《Nature Chemistry》上发表的研究工作，巧妙借鉴已获临床批准的磁热疗技术，通过设计磁性球形核酸，首次在合成细胞内以临床可耐受的交变磁场参数实现了对无细胞蛋白合成的精准调控及封装物释放。该工作展示了交变磁场穿透不透明屏障并通过磁热转换激活合成细胞的独特能力，凸显了磁场作为调控手段在组织穿透深度上无可比拟的优势。结合Michael J. Booth课题组的研究工作，本文围绕外部刺激介导的远程调控策略，系统梳理了光、温度、磁场等刺激模式在合成细胞功能调控中的研究进展，深入剖析了磁控合成细胞系统的创新设计与潜在局限，并结合本课题组在光驱动合成原细胞以及自调控人工β细胞等方面的研究积累，从刺激响应机制、生物-非生物杂化系统以及多模态正交调控等角度，对磁场调控合成细胞技术从概念验证走向实际应用所面临的挑战与未来发展进行了分析与展望。我们认为，未来合成细胞的跨越式发展不仅有赖于单一刺激响应模块的性能提升，更取决于多种控制形式间的高效耦合与协同调控，最终实现具备复杂信息处理能力的智能合成生命系统。

Abstract

Synthetic cells refer to biomimetic systems that are artificially constructed from the bottom up using basic molecular components such as DNA

proteins

and lipids through bioengineering techniques

and that are capable of performing functions analogous to those of natural life. They are emerging as a cutting-edge platform for studying the origin of life and developing intelligent diagnostic and therapeutic devices. A key challenge is the development of remote

noninvasive control strategies that function with deep tissue penetration. This requirement remains difficult to satisfy using light or chemical stimuli due to limited penetration depth and pharmacokinetic constraints. A recent study by Michael J. Booth and co-workers at the University of Oxford addressed this limitation by repurposing clinically established magnetic hyperthermia. The authors engineered magnetic spherical nucleic acids in which T7 promoter duplexes are covalently conjugated to superparamagnetic iron oxide nanoparticles. Exposure to clinically relevant alternating magnetic fields induces localized heating that denatures the duplex

releasing the T7 promoter strand. This process activates cell-free protein synthesis and cargo release within giant vesicles

even behind opaque barriers. This comment places this advance within the broader field of physically responsive synthetic cells and critically evaluates the design principles and intrinsic limitations of magnetic control strategies. Building on our previous work and recent advances on responsive synthetic cells

we discuss how these systems can transduce external physical stimuli into defined biochemical outputs. We further highlight key challenges

including thermal safety constraints

long-term signal stability

reversibility of cargo release

and translational barriers associated with manufacturing

in vivo robustness

and regulatory approval. We propose that next-generation synthetic cells will require multimodal and orthogonal regulatory architectures that integrate the deep tissue penetration of magnetic fields with the spatial precision of light and the adaptability of chemical sensing systems.

关键词

Keywords

references

SAMANTA A , BARANDA PELLEJERO L , MASUKAWA M , et al . DNA-empowered synthetic cells as minimalistic life forms [J ] . Nature Reviews Chemistry , 2024 , 8 ( 6 ): 454 - 470 .

丁明珠 , 李炳志 , 王颖 , 谢泽雄 , 刘夺 , 元英进 . 合成生物学重要研究方向进展 [J ] . 合成生物学 , 2020 , 1 ( 1 ): 7 - 28 .

Ding M Z , Li B Z , Wang Y , XIE Z X , LIU D , YUAN Y J . Significant research progress in synthetic biology [J ] . Synthetic Biology Journal , 2020 , 1 ( 1 ): 7 - 28 .

MOGHIMIANAVVAL H , NEWELL C , PARVIZIAN P , et al . Strategies and applications of synthetic cell communication [J ] . Nature Chemical Biology , 2025 , 21 ( 9 ): 1317 - 1329 .

FROHN B P , KOHYAMA S , SCHWILLE P . Protein design and optimization for synthetic cells [J ] . Nature Reviews Bioengineering , 2025 , 3 ( 8 ): 645 - 659 .

刘明煜 , 罗博煜 , 魏子群 , 张家宁 , 袁刘天韵 , 于小淼 , 张劢 , 贾尚 , 滕越 , 史志远 . 人工细胞在合成生物学中的应用 [J ] . 生物工程学报 , 2025 , 41 ( 5 ): 1705 - 1719 .

LIU M Y , LUO B Y , WEI Z Q , ZHANG J N , YUAN L T Y , YU X M , ZHANG L , JIA S , TENG Y , SHI Z Y . Advancements in the application of artificial cells in synthetic biology [J ] . Chinese Journal of Biotechnology , 2025 , 41 ( 5 ): 1705 - 1719 .

闫玉玲 , 张敏 , 鞠秀琴 , 刘剑波 . 自下而上构建人工细胞及其生物医学应用 [J ] . 集成技术 , 2023 , 13 ( 4 ): 30 - 50 .

YAN Y L , ZHANG M , JU X Q , LIU J B . Bottom-up construction of artificial cells and their biomedical applications [J ] . Journal of Integration Technology , 2023 , 13 ( 4 ): 30 - 50 .

CHEN Z , WANG Z , GU Z . Bioinspired and Biomimetic Nanomedicines [J ] . Accounts of Chemical Research , 2019 , 52 ( 5 ): 1255 - 1264 .

WEI X , WANG Y , HU H , et al . Wirelessly controlled drug delivery systems for translational medicine [J ] . Nature Reviews Electrical Engineering , 2025 , 2 ( 4 ): 244 - 262 .

RIVNAY J , RAMAN R , ROBINSON J T , et al . Integrating bioelectronics with cell-based synthetic biology [J ] . Nature Reviews Bioengineering , 2025 , 3 ( 3 ): 317 - 332 .

CHEN Z , DE QUEIROS SILVEIRA G , MA X , et al . Light-Gated Synthetic Protocells for Plasmon-Enhanced Chemiosmotic Gradient Generation and ATP Synthesis [J ] . Angewandte Chemie International Edition , 2019 , 58 ( 15 ): 4896 - 4900 .

CHEN Z , ZHANG H , GUO P , et al . Semi-artificial Photosynthetic CO 2 Reduction through Purple Membrane Re-engineering with Semiconductor [J ] . Journal of the American Chemical Society , 2019 , 141 ( 30 ): 11811 - 11815 .

CHEN Z , WANG J , SUN W , et al . Synthetic beta cells for fusion-mediated dynamic insulin secretion [J ] . Nature Chemical Biology , 2018 , 14 ( 1 ): 86 - 93 .

DWIDAR M , SEIKE Y , KOBORI S , et al . Programmable Artificial Cells Using Histamine-Responsive Synthetic Riboswitch [J ] . Journal of the American Chemical Society , 2019 , 141 ( 28 ): 11103 - 11114 .

MONCK C , ELANI Y , CERONI F . Genetically programmed synthetic cells for thermo-responsive protein synthesis and cargo release [J ] . Nature Chemical Biology , 2024 , 20 ( 10 ): 1380 - 1386 .

SMITH J M , HARTMANN D , BOOTH M J . Engineering cellular communication between light-activated synthetic cells and bacteria [J ] . Nature Chemical Biology , 2023 , 19 ( 9 ): 1138 - 1146 .

ZHU K K , GISPERT CONTAMINA I , CES O , et al . Magnetic Modulation of Biochemical Synthesis in Synthetic Cells [J ] . Journal of the American Chemical Society , 2024 , 146 ( 19 ): 13176 - 13182 .

SHAKERI-ZADEH A , BULTE J W M . Imaging-guided precision hyperthermia with magnetic nanoparticles [J ] . Nature Reviews Bioengineering , 2025 , 3 ( 4 ): 245 - 260 .

YANG J , LU Y , Physical stimuli-responsive cell-free protein synthesis [J ] . Synthetic and Systems Biotechnology , 2020 , 5 ( 4 ): 363 - 368 .

EFRAT Y , TAYAR A M , DAUBE S S , LEVY M , BAR-ZIV R H , Electric-Field Manipulation of a Compartmentalized Cell-Free Gene Expression Reaction [J ] . ACS Synthetic Biology , 2018 , 7 ( 8 ): 1829 - 1833 .

PARKES E , AL SAMAD A , MAZZOTTI G , et al . Magnetic activation of spherical nucleic acids enables the remote control of synthetic cells [J ] . Nature Chemistry , 2025 , 17 ( 10 ): 1505 - 1513 .

WALTER S . German Clinical Trials Register [EB/OL ] . 2020 .

SOUZA G R , MOLINA J R , RAPHAEL R M , OZAWA M G , STARK D J , LEVIN C S , BRONK L F , ANANTA J S , MANDELIN J , GEORGESCU M M , BANKSON J A , GELOVANI J G , KILLIAN T C , ARAP W , PASQUALINI R , Three-dimensional tissue culture based on magnetic cell levitation [J ] . Nature Nanotechnology , 2010 , 5 : 291 - 296 .

GUPTA T , AITHAL S , MISHRIKI S , SAHU R P , GENG F , PURI I K , Label-Free Magnetic-Field-Assisted Assembly of Layer-on-Layer Cellular Structures [J ] . ACS Biomaterials Science & Engineering , 2020 , 6 ( 7 ): 4294 - 4303 .

HARTMANN D , BOOTH M J . Accessible light-controlled knockdown of cell-free protein synthesis using phosphorothioate-caged antisense oligonucleotides [J ] . Communications Chemistry , 2023 , 6 ( 1 ): 59 .

HEMPHILL J , BORCHARDT E K , BROWN K , et al . Optical Control of CRISPR/Cas9 Gene Editing [J ] . Journal of the American Chemical Society , 2015 , 137 ( 17 ): 5642 - 5645 .

LEVSKAYA A , WEINER O D , LIM W A , et al . Spatiotemporal control of cell signalling using a light-switchable protein interaction [J ] . Nature , 2009 , 461 ( 7266 ): 997 - 1001 .

SHAO J , XUE S , YU G , et al . Smartphone-controlled optogenetically engineered cells enable semiautomatic glucose homeostasis in diabetic mice [J ] . Science Translational Medicine , 2017 , 9 ( 387 ): eaal2298 .

MILLER I C , GAMBOA CASTRO M , MAENZA J , et al . Remote Control of Mammalian Cells with Heat-Triggered Gene Switches and Photothermal Pulse Trains [J ] . ACS Synthetic Biology , 2018 , 7 ( 4 ): 1167 - 1173 .

JIA H , HEYMANN M , HÄRTEL T , et al . Temperature-sensitive protein expression in protocells [J ] . Chemical Communications , 2019 , 55 ( 45 ): 6421 - 6424 .

BOLINGER P Y , STAMOU D , VOGEL H . Integrated Nanoreactor Systems: Triggering the Release and Mixing of Compounds Inside Single Vesicles [J ] . Journal of the American Chemical Society , 2004 , 126 ( 28 ): 8594 - 8595 .

CHERTOK B , LANGER R . Circulating Magnetic Microbubbles for Localized Real-Time Control of Drug Delivery by Ultrasonography-Guided Magnetic Targeting and Ultrasound [J ] . Theranostics , 2018 , 8 ( 2 ): 341 - 357 .

GLEICH B , WEIZENECKER J . Tomographic imaging using the nonlinear response of magnetic particles [J ] . Nature , 2005 , 435 ( 7046 ): 1214 - 1217 .

DIAS J T , MOROS M , DEL PINO P , et al . DNA as a Molecular Local Thermal Probe for the Analysis of Magnetic Hyperthermia [J ] . Angewandte Chemie International Edition , 2013 , 52 ( 44 ): 11526 - 11529 .

BEARDO A , CHEN W , MCBENNETT B , et al . Nanoscale confinement of phonon flow and heat transport [J ] . npj Computational Materials , 2025 , 11 ( 1 ): 172 .

CECCHINI M P , TUREK V A , PAGET J , et al . Self-assembled nanoparticle arrays for multiphase trace analyte detection [J ] . Nature Materials , 2013 , 12 ( 2 ): 165 - 171 .

LEE J H , JANG J T , CHOI J S , et al . Exchange-coupled magnetic nanoparticles for efficient heat induction [J ] . Nature Nanotechnology , 2011 , 6 ( 7 ): 418 - 422 .

浏览量

下载量

CSCD

文章被引用时，请邮件提醒。

提交

工具集

关联资源

限制性内切酶的无细胞快速制备研究

基于无细胞体系的生物合成代谢模块设计、构建与快速途径原型

电催化还原CO2耦合代谢工程改造解脂耶氏酵母实现高效异戊二烯生物合成

食气梭菌驱动的一碳气体增值转化：进展与挑战

基于微生物电合成的一碳生物转化