木聚糖酶Xyn11A与阿拉伯呋喃糖苷酶Abf62A协同水解麦秆木聚糖生物合成低聚木糖

胡蝶; 徐道铸; 鲁志毅; 唐卫; 樊博; 何玉财

doi:10.12211/2096-8280.2025-037

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木聚糖酶Xyn11A与阿拉伯呋喃糖苷酶Abf62A协同水解麦秆木聚糖生物合成低聚木糖

研究论文 | 更新时间：2025-09-05

- 木聚糖酶Xyn11A与阿拉伯呋喃糖苷酶Abf62A协同水解麦秆木聚糖生物合成低聚木糖
- Biosynthesis of xylo-oligosaccharides from wheat straw xylan through the synergistic hydrolysis by xylanase Xyn11A and arabinofuranosidase Abf62A
- 合成生物学 2025年6卷第4期页码：972-986
- 作者机构：
  
  常州大学，药学院生物与食品工程学院，江苏常州 213164
- 作者简介：
  
  [ "胡蝶（1988—），女，博士，助理研究员，硕士生导师。研究方向为酶工程与酶分子设计。 E-mail：Butterflystudy@163.com" ]
  [ "何玉财（1979—），男，博士，教授，博士生导师，研究方向为合成生物学和生物质能源。E-mail：heyucai2001@126.com" ]
- 基金信息：
  
  国家自然科学基金(22208031;21978072;22308034)
- DOI：10.12211/2096-8280.2025-037
  中图分类号： Q814
- 收稿：2025-04-29，
  
  修回：2025-07-03，
  
  纸质出版：2025-08-31
- 稿件说明：
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胡蝶，徐道铸，鲁志毅，唐卫，樊博，何玉财. 木聚糖酶Xyn11A与阿拉伯呋喃糖苷酶Abf62A协同水解麦秆木聚糖生物合成低聚木糖［J］. 合成生物学， 2025， 6（4）： 972-986

HU Die， XU Daozhu， LU Zhiyi， TANG Wei， FAN Bo， HE Yucai. Biosynthesis of xylo-oligosaccharides from wheat straw xylan through the synergistic hydrolysis by xylanase Xyn11A and arabinofuranosidase Abf62A［J］. Synthetic Biology Journal， 2025， 6（4）： 972-986
胡蝶，徐道铸，鲁志毅，唐卫，樊博，何玉财. 木聚糖酶Xyn11A与阿拉伯呋喃糖苷酶Abf62A协同水解麦秆木聚糖生物合成低聚木糖［J］. 合成生物学， 2025， 6（4）： 972-986 DOI： 10.12211/2096-8280.2025-037.

HU Die， XU Daozhu， LU Zhiyi， TANG Wei， FAN Bo， HE Yucai. Biosynthesis of xylo-oligosaccharides from wheat straw xylan through the synergistic hydrolysis by xylanase Xyn11A and arabinofuranosidase Abf62A［J］. Synthetic Biology Journal， 2025， 6（4）： 972-986 DOI： 10.12211/2096-8280.2025-037.

摘要

低聚木糖（XOSs）是一类由2～7个木糖分子通过β-1，4糖苷键连接而成的功能性寡糖，被公认为具有益生元活性的可溶性膳食纤维。在温和条件下的酶催化从廉价生物质提取木聚糖制备高附加值低聚木糖引起了广泛关注。本研究从宇佐美曲霉中克隆了一种阿拉伯呋喃糖苷酶Abf62A基因，并实现其在毕赤酵母X33中的异源表达。利用木聚糖酶Xyn11A（300 U/g 底物）和阿拉伯呋喃糖苷酶Abf62A（20 U/g 底物）对高浓度麦秆木聚糖（100 g/L）进行协同酶水解，生成50.32 g/L低聚木糖，相较于木聚糖酶Xyn11A单酶水解（34.42 g/L低聚木糖）展现了显著的协同效应。协同水解低聚木糖产物分析结果显示50.32 g/L低聚木糖中包含木二糖（31.71 g/L）、木三糖（15.92 g/L）、木四糖（1.65 g/L）和木五糖（1.04 g/L），其中木二糖和木三糖的含量高达94.7%。此外，从纯化后酶解低聚木糖产物显示可有效清除自由基，抗氧化活性>90%。综上所述，本研究通过木聚糖酶和阿拉伯呋喃糖苷酶Abf62A的协同生物催化作用，以绿色可持续的方式从麦秆木聚糖中制备低聚木糖，为人类和动物健康提供了一种益生元寡糖。

Abstract

Xylo-oligosaccharides (XOSs) are a category of functional oligosaccharides primarily composed of 2-7 xylose units linked by β-1

4 glycosidic bonds. They are recognized as soluble dietary fibers with prebiotic properties. Recently

there has been significant interest in manufacturing XOSs from xylan extracted from lignocellulosic biomass using enzyme catalysis under mild conditions. In this work

the arabinofuranosidase Abf62A gene was cloned from

Aspergillus usamii

genomic DNA through sequential molecular processes and expressed in

Pichia pastoris

X33. The xylan (100 g/L) extracted xylan in wheat straw (WS) was biologically hydrolyzed into 50.32 g/L of XOSs by xylanase Xyn11A (300 U/g substrate) and arabinofuranase Abf62A (20 U/g substrate)

which indicated a notable synergistic effect compared to the 34.42 g/L XOSs produced

via

Xyn11A. The 50.32 g/L of XOSs products comprised xylobiose (31.71 g/L)

xylotriose (15.92 g/L)

xylotetraose (1.65 g/L) and xylopentaose (1.04 g/L). Notably

the combined content of xylobiose and xylotriose accounted for up to 94.7%. The XOSs purified from the enzyme hydrolysate could effectually scavenge free radicals

and the antioxidant activity was more than 90%. In summary

XOSs were biologically manufactured from whe

at straw xylan through the synergistic biocatalysis via xylanase and arabinofuranosidase Abf62A in a green and sustainable way

rending one kind of prebiotic oligosaccharides with substantial positive effects on human and animal health.

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references

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