大肠杆菌中吲哚介导的双向趋化行为的调控与分析

汪昆昆; 陈泓宇; 张安栋; 卢晓云; 谭丹

doi:10.12211/2096-8280.2025-036

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大肠杆菌中吲哚介导的双向趋化行为的调控与分析

研究论文 | 更新时间：2026-01-15

- 大肠杆菌中吲哚介导的双向趋化行为的调控与分析
- A preliminary study on the regulation and analysis of indole mediated bidirectional chemotaxis behavior of Escherichia coli
- 合成生物学 2025年6卷第6期页码：1398-1420
- 作者机构：
  
  1.西安交通大学生命科学与技术学院，生物医学信息工程教育部重点实验室，陕西西安 710049
  2.清华大学生命科学学院，北京 100084
- 作者简介：
  
  [ "汪昆昆（1999—），男，硕士研究生。研究方向为基于嗜盐微生物的合成生物学。E-mail：3122113016@stu.xjtu.edu.cn" ]
  [ "谭丹（1986—），女，博士，副教授，硕士生导师。研究方向为基于嗜盐微生物的合成生物学与定量生物学、新活性天然产物的生物合成及关键酶挖掘与研究等。E-mail：tandan@mail.xjtu.edu.cn" ]
- 基金信息：
- DOI：10.12211/2096-8280.2025-036
  中图分类号： Q819
- 收稿：2025-04-29，
  
  修回：2025-07-31，
  
  纸质出版：2025-12-31
- 稿件说明：
移动端阅览
汪昆昆，陈泓宇，张安栋，卢晓云，谭丹. 大肠杆菌中吲哚介导的双向趋化行为的调控与分析［J］. 合成生物学， 2025， 6（6）： 1398-1420

WANG Kunkun， CHEN Hongyu， ZHANG Andong， LU Xiaoyun， TAN Dan. A preliminary study on the regulation and analysis of indole mediated bidirectional chemotaxis behavior of Escherichia coli［J］. Synthetic Biology Journal， 2025， 6（6）： 1398-1420
汪昆昆，陈泓宇，张安栋，卢晓云，谭丹. 大肠杆菌中吲哚介导的双向趋化行为的调控与分析［J］. 合成生物学， 2025， 6（6）： 1398-1420 DOI： 10.12211/2096-8280.2025-036.

WANG Kunkun， CHEN Hongyu， ZHANG Andong， LU Xiaoyun， TAN Dan. A preliminary study on the regulation and analysis of indole mediated bidirectional chemotaxis behavior of Escherichia coli［J］. Synthetic Biology Journal， 2025， 6（6）： 1398-1420 DOI： 10.12211/2096-8280.2025-036.

摘要

细菌趋化性在肠道菌群定植和癌症诊疗等领域发挥着重要作用，也是定量生物学与合成生物学的研究方向之一。大肠杆菌中吲哚诱导的趋化反应主要由两种效应相反的甲基化趋化受体Tsr与Tar介导，两者的拮抗效应可导致双向趋化反应的发生。本文从数学模型的建立与仿真以及实验调控与观测两方面对该双向趋化反应进行研究。基于信号转导动力学与马尔可夫随机游走模型，分别从个体水平和群体水平构建了大肠杆菌在双受体介导下趋化性的数学模型，并对不同受体比例下大肠杆菌的趋化响应行为进行了模拟和仿真分析。同时，利用CRISPRi技术对Tar受体进行了不同程度的可诱导敲低（分别敲低0%、40%和70%），并借助于Transwell细菌迁移实验和自主设计优化的微流控观测平台，对吲哚介导下不同Tar表达量菌株的趋化性行为进行了观测与分析。通过两种甲基化趋化受体Tsr/Tar相对比例的调节实现了细菌趋化行为的调控，同时结合数学建模初步揭示了相互拮抗的两种趋化性受体比例对细菌个体和群体趋化运动的影响，为扩展趋化行为的定量研究和精确调控手段，以及未来细菌趋化性在肠道菌群人工调节等多领域的应用奠定基础。

Abstract

Chemotaxis constitutes a fundamental mechanism underlying bacterial behaviors such as microbiota colonization and cancer therapy

while also representing an actively studied area within quantitative and synthetic biology. The indole induced bidirectional chemotaxis in

Escherichia coli

is mainly mediated by two methylation chemoreceptors (MCPs) with antagonistic effects

Tsr and Tar respectively

which was investigated in this study by mathematical model and experimental verification. Based on signal transduction dynamics model and Markov random walk model

the bidire

ctional chemotaxis of

E. coli

was described and simulated at both individual and population levels. Meanwhile

one of the MCPs receptors Tar was knockdown by 0%

40% and 70% respectively

via

CRISPRi

and the chemotaxis behaviors of knockdown strains with different Tar expression levels under indole induction were observed and analyzed by Transwell migration experiment and self-designed microfluidics platform. The regulation of bacterial chemotaxis behavior was achieved in this study by adjusting the ratio of two antagonistic chemotaxis receptors Tsr and Tar

and together with mathematical model and experimental observation

the influence of Tsr/Tar ratio on the chemotaxis behavior of

E. coli

at individual and population level was preliminarily revealed in our study. This will benefit the quantitative analysis and precise regulation of chemotaxis behavior

as well as the future applications of bacterial chemotaxis in various fields.

关键词

Keywords

references

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