酚酸类化合物：从植物提取到生物合成

李星烨; 胡楠

doi:10.12211/2096-8280.2025-018

您当前的位置：

首页 >

文章列表页 >

酚酸类化合物：从植物提取到生物合成

特约评述 | 更新时间：2026-01-15

- 酚酸类化合物：从植物提取到生物合成
- Phenolic acid compounds： extraction from plants to biosynthesis
- 合成生物学 2025年6卷第6期页码：1367-1383
- 作者机构：
  
  中北大学化学与化工学院生物工程系，山西太原 030051
- 作者简介：
  
  [ "李星烨（2004—），男，本科生。研究方向为酚酸类化合物的植物提取。所在NUC-CHINA团队荣获2024年合成生物学创新赛蛋白设计赛金奖。 E-mail：13284156338@163.com" ]
  [ "胡楠（1992—），男，副教授，硕士生导师。研究方向为生物分离。 E-mail：nanhu@nuc.edu.cn" ]
- 基金信息：
  
  国家自然科学基金(22108260)
- DOI：10.12211/2096-8280.2025-018
  中图分类号： TQ28
- 收稿：2025-03-19，
  
  修回：2025-07-07，
  
  纸质出版：2025-12-31
- 稿件说明：
移动端阅览
李星烨，胡楠. 酚酸类化合物：从植物提取到生物合成［J］. 合成生物学， 2025， 6（6）： 1367-1383

LI Xingye， HU Nan. Phenolic acid compounds： extraction from plants to biosynthesis［J］. Synthetic Biology Journal， 2025， 6（6）： 1367-1383
李星烨，胡楠. 酚酸类化合物：从植物提取到生物合成［J］. 合成生物学， 2025， 6（6）： 1367-1383 DOI： 10.12211/2096-8280.2025-018.

LI Xingye， HU Nan. Phenolic acid compounds： extraction from plants to biosynthesis［J］. Synthetic Biology Journal， 2025， 6（6）： 1367-1383 DOI： 10.12211/2096-8280.2025-018.

摘要

酚酸类化合物，作为一类广泛存在于植物中的次生代谢产物，展现出抗氧化、抗炎、抗癌及抑菌等多种生物活性。随着研究者对这类化合物了解的不断加深，其在食品、医药和化妆品等多个领域的应用潜力逐渐显现。传统上，从植物中提取酚酸类化合物是获取此类物质的主要途径；然而，由于受到效率低下与纯度不足等因素制约，加之提取过程耗时较长，这种方法正面临难以满足市场日益增长需求的问题。本文总结了近年来逐步受到重视的基于基因工程与代谢工程技术的新型生产方法——生物合成技术在酚酸类化合物中的应用。该技术不仅能够显著提升酚酸类化合物的产量与品质，还实现了更加环保高效的制备流程。尽管如此，有关酚酸类化合物生物合成的具体机制仍有待进一步阐明，特别是不同合成路径之间调控机制尚不完全清楚。此外，如何提高这些化合物的生物利用度及其稳定性也是当前亟需解决的关键挑战之一。因此，未来的研究工作应当致力于揭示更多关于酚酸类化合物生物合成过程的信息，并探索更为先进的合成策略，以促进其更广泛的应用与发展。

Abstract

Phenolic acid compounds are a class of secondary metabolites that are widely distributed in the plant kingdom

where they play essential roles in plant growth

development

and defense mechanisms. These compounds are known for their diverse bioactivities

including antioxidants

anti-inflammatory

anticancer

and antimicrobial properties

making them valuable natural products with significant therapeutic potential. In recent years

as understanding of their biological functions has deepened through advanced analytical techniques and molecular biology studies

phenolic acid compounds have shown increasingly promising potential for the applications in the fields of food preservation

nutraceuticals

pharmaceuticals

and cosmetics

particularly as natural alternatives to synthetic additives. However

the traditional method of obtaining these compounds through plant extraction is often limited by factors such as extraction efficiency

purity

and scalability

which are influenced by seasonal variations

plant species differences

and extraction methods. Recently

biosynthesis has emerged as a novel and promising approach to produce phenolic acid compounds in a more sustainable and controllable manner

addressing many of the limitations associated with conventional extraction methods. This review summarizes the application of biosynthesis in phenolic acid compounds production

focusing on recent advances in microbial fermentation and plant cell culture technologies. By employing genetic engineering and metabolic engineering techniques

including gene knockout

overexpression

and pathway optimization strategies

it is possible to significantly enhance the yield and purity of these compounds in various biological systems. For example

the overexpression of key enzymes involved in the phenylpropanoid pathway

such as phenylalanine ammonia-lyase (PAL) and cinnamate-4-hydroxylase (C4H)

can lead to increased production of phenolic acids in both microbial and plant hosts. However

there are still many unknowns regarding the biosynthetic mechanisms of phenolic acids that require further investigation

particularly the pathway regulation and metabolic flux control. The regulatory mechanisms of different biosynthetic pathways and their expression variations among various plant species remain to be fully elucidated through comprehensive omics studies and comparative genomics approaches. In addition to biosynthesis challenges

the bioavailability and stability of phenolic acid compounds remain critical challenges that need to be addressed for their practical applications in commercial products. Phenolic acids are often prone to be degradative under certain conditions such as high temperature

extreme pH

or prolonged storage

which can limit their effectiveness and shelf-life in final formulations. Therefore

future research should focus on exploring the biosynthetic pathways of phenolic acid compounds in greater details using systems biology approaches

optimizing extraction and purification techniques to improve efficiency and purity through innovative separation technologies

and developing efficient biosynthetic systems using synthetic biology tools and high-throughput screening methods. These efforts will be crucial in realizing the widespread application of phenolic acid compounds across multiple fields including medicine

agriculture

food industry

and personal care products

ultimately contributing to the development of sustainable bioprocesses and value-added natural products.

关键词

Keywords

references

刘苹 , 赵海军 , 唐朝辉 , 等 . 连作对不同抗性花生品种根系分泌物和土壤中化感物质含量的影响 [J ] . 中国油料作物学报 , 2015 , 37 ( 4 ): 467 - 474 .

LIU P , ZHAO H J , TANG Z H , et al . Effects of continuous cropping on root exudates of different resistance peanut ( Arachis hypogaea L.) varieties and allelochemicals content in soil [J ] . Chinese Journal of Oil Crop Sciences , 2015 , 37 ( 4 ): 467 - 474 .

李树白 , 聂华丽 , 薛勇 , 等 . 对羟基苯乙酸在酪氨酸酶催化中的动力学研究 [J ] . 化学试剂 , 2010 , 32 ( 2 ): 97 - 101 .

LI S B , NIE H L , XUE Y , et al . Inhibitory kinetics of p -hydroxyphenylacetic acid on mushroom tyrosinase-catalyzing reaction [J ] . Chemical Reagents , 2010 , 32 ( 2 ): 97 - 101 .

于倩 . 返魂草颗粒中对羟基苯甲酸和对羟基苯乙酸的含量测定及药代动力学研究 [D ] . 大连 : 大连医科大学 , 2016 .

YU Q . Content determination and pharmacokinetics of hydroxy benzoic acid and benzoic acid in the fanhuncao granule [D ] . Dalian : Dalian Medical University , 2016 .

KUMAR N , GOEL N . Phenolic acids: natural versatile molecules with promising therapeutic applications [J ] . Biotechnology Reports , 2019 , 24 : e00370 .

杨阳 , 何师国 . 葱属植物化感作用研究进展 [J ] . 北方园艺 , 2016 ( 3 ): 189 - 194 .

YANG Y , HE S G . Research progress on Allium allelopathy [J ] . Northern Horticulture , 2016 ( 3 ): 189 - 194 .

张东明 . 酚酸化学 [M ] . 北京 : 化学工业出版社 , 2009 .

ZHANG D M . Phenolic acid chemistry [M ] . Beijing : Chemical Industry Press , 2009 .

石梦瑶 , 赵薇 , 杨安全 , 等 . 酚酸类化合物在化妆品中的原料制备与应用 [J ] . 精细化工 , 2024 , 41 ( 2 ): 245 - 256, 276 .

SHI M Y , ZHAO W , YANG A Q , et al . Preparation and application of phenolic acid compounds as raw materials in cosmetics [J ] . Fine Chemicals , 2024 , 41 ( 2 ): 245 - 256, 276 .

DEL CARMEN VILLEGAS-AGUILAR M , SÁNCHEZ-MARZO N , FERNÁNDEZ-OCHOA Á , et al . Evaluation of bioactive effects of five plant extracts with different phenolic compositions against different therapeutic targets [J ] . Antioxidants , 2024 , 13 ( 2 ): 217 .

陈志杰 , 吴嘉琪 , 马燕 , 等 . 植物食品原料中酚酸的生物合成与调控及其生物活性研究进展 [J ] . 食品科学 , 2018 , 39 ( 7 ): 321 - 328 .

CHEN Z J , WU J Q , MA Y , et al . Biosynthesis, metabolic regulation and bioactivity of phenolic acids in plant food materials [J ] . Food Science , 2018 , 39 ( 7 ): 321 - 328 .

GROMOVAYA V F , SHAPOVAL G S , MIRONYUK I E . Antiradical and antioxidant activity of biologically active carboxylic acids [J ] . Russian Journal of General Chemistry , 2002 , 72 ( 5 ): 774 - 777 .

GAO Q C , LI Y , LI Y H , et al . Antioxidant and prooxidant activities of phenolic acids commonly existed in vegetables and their relationship with structures [J ] . Food Science and Technology , 2022 , 42 : e07622 .

KOCH W , KUKUŁA-KOCH W , CZOP M , et al . The role of extracting solvents in the recovery of polyphenols from green tea and its antiradical activity supported by principal component analysis [J ] . Molecules , 2020 , 25 ( 9 ): 2173 .

ZALA A R , RAJANI D P , KUMARI P . Synthesis, molecular docking, ADME study, and antimicrobial potency of piperazine based cinnamic acid bearing coumarin moieties as a DNA gyrase inhibitor [J ] . Journal of Biochemical and Molecular Toxicology , 2023 , 37 ( 1 ): e23231 .

MERMER A , KELES T , SIRIN Y . Recent studies of nitrogen containing heterocyclic compounds as novel antiviral agents: a review [J ] . Bioorganic Chemistry , 2021 , 114 : 105076 .

FLORIS B , GALLONI P , CONTE V , et al . Tailored functionalization of natural phenols to improve biological activity [J ] . Biomolecules , 2021 , 11 ( 9 ): 1325 .

GOMES C A , GIRÃO DA CRUZ T , ANDRADE J L , et al . Anticancer activity of phenolic acids of natural or synthetic origin:a structure-activity study [J ] . Journal of Medicinal Chemistry , 2003 , 46 ( 25 ): 5395 - 5401 .

GODLEWSKA-ŻYŁKIEWICZ B , ŚWISŁOCKA R , KALINOWSKA M , et al . Biologically active compounds of plants: structure-related antioxidant, microbiological and cytotoxic activity of selected carboxylic acids [J ] . Materials , 2020 , 13 ( 19 ): 4454 .

WANG C , WANG H , ZHAO Z J , et al . Pediococcus acidilactici AS185 attenuates early atherosclerosis development through inhibition of lipid regulation and inflammation in rats [J ] . Journal of Functional Foods , 2019 , 60 : 103424 .

CHATATIKUN M , KWANHIAN W . Phenolic profile of Nipa palm vinegar and evaluation of its antilipidemic activities [J ] . Evidence-Based Complementary and Alternative Medicine , 2020 , 2020 ( 1 ): 6769726 .

CHENG Y H , WANG Y T , YUAN T J , et al . Polyphenol compounds contributing to the improved bioactivities of fermented Rubus chingii Hu [J ] . Food Research International , 2024 , 197 : 115218 .

李京芳 . 莲藕酶促褐变及功能成分变化对降脂、降胆固醇的作用和机理探讨 [D ] . 南昌 : 南昌大学 , 2023 .

LI J F . Effects of the enzymatic browning of Lotus root and the transformation of functional components on regulating lipid and cholesterol metabolism [D ] . Nanchang : Nanchang University , 2023 .

胡勇梅 . 植物多酚及其衍生物的抗菌活性评价与作用机制研究 [D ] . 兰州 : 兰州大学 , 2023 .

HU Y M . Evaluation of antimicrobial activity and mechanism of plant polyphenols and their derivatives [D ] . Lanzhou : Lanzhou University , 2023 .

王静 , 丁海燕 . 酚酸类化合物抑菌作用研究进展 [J ] . 中成药 , 2022 , 44 ( 6 ): 1906 - 1911 .

WANG J , DING H Y . Research progress on the antibacterial effect of phenolic acids [J ] . Chinese Traditional Patent Medicine , 2022 , 44 ( 6 ): 1906 - 1911 .

SARANKAR S K , RAJAK B , OJHA S , et al . Exploring the utilization of phenolic compounds in pharmaceuticals and healthcare [J ] . GSC Biological and Pharmaceutical Sciences , 2023 , 24 ( 3 ): 95 - 102 .

ZHAO N , LIU Z Y , YU T , et al . Spent coffee grounds: present and future of environmentally friendly applications on industries-a review [J ] . Trends in Food Science & Technology , 2024 , 143 : 104312 .

WANG M , WANG Z Y , ZHANG J C , et al . Sustainable bioactive salts fully composed of natural products for enhanced pharmaceutical applicability [J ] . ACS Sustainable Chemistry & Engineering , 2022 , 10 ( 31 ): 10369 - 10382 .

李莎 , 张培卫 , 王翔 . 医药抗癌苯并吡咯及其衍生物的合成研究进展 [J ] . 凯里学院学报 , 2012 , 30 ( 3 ): 42 - 47 .

LI S , ZHANG P W , WANG X . Research progress on synthesis of medicine anticancer benzene and pyrrole and its derivatives [J ] . Journal of Kaili University , 2012 , 30 ( 3 ): 42 - 47 .

ROY A , CHINTA J P . Antioxidant conjugated metal complexes and their medicinal applications [M ] // Vitamins and hormones . Amsterdam : Elsevier , 2023 : 319 - 353 .

孔令义 . 天然药物化学 [M ] . 北京 : 化学工业出版社 , 2018 .

KONG L Y . Natural medicinal chemistry [M ] . Beijing : Chemical Industry Press , 2018 .

岳春磊 . 代谢工程改造酿酒酵母合成迷迭香酸及其前体的研究 [D ] . 扬州 : 扬州大学 , 2022 .

YUE C L . Metabolic engineering of Saccharomyces cerevisiae to synthesize rosmarinic acid and its precursors [D ] . Yangzhou : Yangzhou University , 2022 .

LIANG X X , FAN Q J . Application of sub-critical water extraction in pharmaceutical industry [J ] . Journal of Materials Science and Chemical Engineering , 2013 , 1 ( 5 ): 1 - 6 .

王瑞红 . 过表达 Sm PsbD对丹参光合作用和酚类化合物生物合成的影响作用研究 [D ] . 咸阳 : 西北农林科技大学 , 2020 .

WANG R H . Effects of over-expression of Sm PsbD on photosynthesis and biosynthesis of phenolic compounds in Salvia miltiorrhiza [D ] . Xianyang : Northwest A & F University , 2020 .

郭娜 . 桑叶和桑果活性成分提取与微生物转化研究 [D ] . 哈尔滨 : 东北林业大学 , 2021 .

GUO N . Study on extraction and microbial transformation of active components from mulberry leaves and mulberry fruits [D ] . Harbin : Northeast Forestry University , 2021 .

王静 . 苍山地区地茶属地衣内生真菌的分离及活性菌株 Coprinopsis sp . Ts-F016抗MRSA次级代谢产物初步研究[D ] . 大理 : 大理大学 , 2023 .

WANG J . Isolation of endophytic fungi from the lichen genus Thamnolia spp . in Cangshan region and preliminary study on secondary metabolites of active strain Coprinopsis sp. Ts-F016 against MRSA[D ] . Dali : Dali University , 2023 .

MARCHIOSI R , DOS SANTOS W D , CONSTANTIN R P , et al . Biosynthesis and metabolic actions of simple phenolic acids in plants [J ] . Phytochemistry Reviews , 2020 , 19 ( 4 ): 865 - 906 .

Phenolics and flavonoids [C ] // Abstracts of 14th World Congress of Food Science & Technology , 2008 : 23 .

CARPENTIERI S , FERRARI G , PATARO G . Pulsed electric fields-assisted extraction of valuable compounds from red grape pomace: process optimization using response surface methodology [J ] . frontiers in Nutrition , 2023 , 10 : 1158019 .

HAMMOUD M , DEBS E , VAN DEN BROEK L A M , et al . Intensification of extraction process through IVDV pretreatment from Eryngium creticum leaves and stems: maximizing yields and assessing biological activities [J ] . Heliyon , 2024 , 10 ( 6 ): e27431 .

DONG J H , CHEN X Y , WANG C Y , et al . Ultra-fast and selective extraction of phenolic acids by multifunctional and magnetic nanomaterials in honeysuckle [J ] . Journal of Chromatography A , 2025 , 1755 : 466028 .

OSAMEDE AIROUYUWA J , SIVAPRAGASAM N , ALI REDHA A , et al . Sustainable green extraction of anthocyanins and carotenoids using deep eutectic solvents (DES): a review of recent developments [J ] . Food Chemistry , 2024 , 448 : 139061 .

DJAOUDENE O , BACHIR-BEY M , SCHISANO C , et al . A sustainable extraction approach of phytochemicals from date ( Phoenix dactylifera L.) fruit cultivars using ultrasound-assisted deep eutectic solvent: a comprehensive study on bioactivity and phenolic variability [J ] . Antioxidants , 2024 , 13 ( 2 ): 181 .

MOKHTARI B , POURABDOLLAH K . Inclusion extraction of alkali metals by emulsion liquid membranes bearing nano-baskets [J ] . Journal of Inclusion Phenomena and Macrocyclic Chemistry , 2013 , 76 ( 3 ): 403 - 413 .

ZHOU B , YAN S H , LI Y . Expression of anthocyanin biosynthetic genes during fruit development in ‘Fengxiang’ Strawberry [M/OL ] // Advanced materials research: future material research and industry application(FMRIA 2011 Part 1) . Zurich, Switzerland: Trans Tech Publications Ltd, 2011 , 455-456 : 443 - 448 [2025-01-01] . https://doi.org/10.4028/www.scientific.net/AMR.455-456.443 https://doi.org/10.4028/www.scientific.net/AMR.455-456.443 .

朱艳琴 . 基于代谢组学及转录组学对不同生境的辣木叶化学多样性研究 [D ] . 昆明 : 昆明理工大学 , 2021 .

ZHU Y Q . Study on the chemical diversity of Moringa oleifera leaves in different habitats based on metabolomics and transcriptomics [D ] . Kunming : Kunming University of Science and Technology , 2021 .

刘欣瑜 . 西藏3种蒿属植物代谢组学分析及其生物活性研究 [D ] . 咸阳 : 西北农林科技大学 , 2023 .

LIU X Y . Metabolomic analysis and biological activity of three Artemisia species in Tibet [D ] . Xianyang : Northwest A & F University , 2023 .

HAJMOHAMMADI M R , NAJAFI ASLIPASHAKI S , RAJAB DIZAVANDI Z , et al . Ultrasound-assisted vesicle-based microextraction as a novel method for determination of phenolic acid compounds in Nepeta cataria L. samples [J ] . Journal of the Iranian Chemical Society , 2021 , 18 ( 7 ): 1559 - 1566 .

李辉 , 李佑稷 , 蒋剑波 . 植物天然产物提取工艺学 [M ] . 北京 : 化学工业出版社 , 2022 .

LI H , LI Y J , JIANG J B . Extraction technology of plant natural products [M ] . Beijing : Chemical Industry Press , 2022 .

WANG X H , CAI C , LI X M . Optimal extraction of gallic acid from Suaeda glauca bge. leaves and enhanced efficiency by ionic liquids [J ] . International Journal of Chemical Engineering , 2016 , 2016 ( 1 ): 5217802 .

YANG Z , YUE S J , GAO H , et al . Natural deep eutectic solvent-ultrasound assisted extraction: a green approach for ellagic acid extraction from Geum japonicum [J ] . Frontiers in Nutrition , 2023 , 9 : 1079767 .

AHMAD A . A box-behnken design for optimization of ultrasound-assisted extraction of sinapic acid from Fragaria ananassa [J ] . Farmacia , 2023 , 71 ( 5 ): 938 - 947 .

KUŚ P , JERKOVIĆ I , JAKOVLJEVIĆ M , et al . Extraction of bioactive phenolics from black poplar ( Populus nigra L.) buds by supercritical CO 2 and its optimization by response surface methodology [J ] . Journal of Pharmaceutical and Biomedical Analysis , 2018 , 152 : 128 - 136 .

AMIRAH , REDDY PRASAD D M , KHAN M R . Comparison of extraction techniques on extraction of gallic acid from stem bark of Jatropha curcas [J ] . Journal of Applied Sciences , 2012 , 12 ( 11 ): 1106 - 1111 .

PANAGIOTIDOU C , BOULOUMPASI E , IRAKLI M , et al . Optimization of extracted phenolic compounds from oregano through accelerated solvent extraction using response surface methodology [J ] . Engineering Proceedings , 2024 , 67 ( 1 ): 10 .

高皊 . 超声辅助柑橘皮提取物绿色合成纳米金颗粒及其抗炎活性研究 [D ] . 镇江 : 江苏大学 , 2022 .

GAO L . Ultrasound-assisted green synthesis of gold nanoparticles using citrus peel extract and the anti-inflammatory activity [D ] . Zhenjiang : Jiangsu University , 2022 .

BOY F R , CASQUETE R , MARTÍNEZ A , et al . Antioxidant, antihypertensive and antimicrobial properties of phenolic compounds obtained from native plants by different extraction methods [J ] . International Journal of Environmental Research and Public Health , 2021 , 18 ( 5 ): 2475 .

WANG L , BOUSSETTA N , LEBOVKA N , et al . Selectivity of ultrasound-assisted aqueous extraction of valuable compounds from flesh and peel of apple tissues [J ] . LWT-Food Science and Technology , 2018 , 93 : 511 - 516 .

SANTOS-SÁNCHEZ N F , SALAS-CORONADO R , HERNÁNDEZ-CARLOS B , et al . Shikimic acid pathway in biosynthesis of phenolic compounds [M ] // Plant physiological aspects of phenolic compounds . London : IntechOpen , 2019 .

陈鑫洁 , 钱芷兰 , 刘启 , 等 . 毕赤酵母底盘芳香族氨基酸合成途径改造生产肉桂酸及对香豆酸 [J ] . 中国生物工程杂志 , 2021 , 41 ( 10 ): 52 - 61 .

CHEN X J , QIAN Z L , LIU Q , et al . Modification of aromatic amino acid synthetic pathway i n Pichia pastoris to produce cinnamic acid and ρ -coumaric acid [J ] . China Biotechnology , 2021 , 41 ( 10 ): 52 - 61 .

李园子 , 谢琴 , 廖永红 , 等 . 微生物合成阿魏酸的研究进展 [J ] . 中国生物工程杂志 , 2024 , 44 ( 9 ): 113 - 125 .

LI Y Z , XIE Q , LIAO Y H , et al . Advances in ferulic acid production by engineered microorganisms [J ] . China Biotechnology , 2024 , 44 ( 9 ): 113 - 125 .

屈艳君 , 王文慧 , 曹家南 , 等 . 鞣花酸的制备及应用研究进展 [J ] . 中国食物与营养 , 2022 , 28 ( 6 ): 39 - 45 .

QU Y J , WANG W H , CAO J N , et al . Research progress on preparation and application of ellagic acid [J ] . Food and Nutrition in China , 2022 , 28 ( 6 ): 39 - 45 .

李宁 , 宋春明 . 医药中间体β-对羟基苯乙醇合成与工艺研究 [J ] . 化工管理 , 2017 ( 26 ): 65 .

LI N , SONG C M . Research on the synthesis and process of pharmaceutical intermediates β- p -hydroxyphenylethanol [J ] . Chemical Enterprise Management , 2017 ( 26 ): 65 .

谈荣慧 , 赵望 , 张金家 , 等 . 丹参 Sm HPPR1基因调控丹酚酸生物合成的研究 [J ] . 药学学报 , 2023 , 58 ( 9 ): 2818 - 2828 .

TAN R H , ZHAO W , ZHANG J J , et al . Study on the regulation of salvianolic acid biosynthesis by Sm HPPR1 gene in Salvia miltiorrhizae [J ] . Acta Pharmaceutica Sinica , 2023 , 58 ( 9 ): 2818 - 2828 .

刘蓉 , 王美燕 , 杜红毅 , 等 . 咖啡酸合成途径重构及其在大肠杆菌中的异源表达 [J ] . 微生物学报 , 2024 , 64 ( 11 ): 4371 - 4387 .

LIU R , WANG M Y , DU H Y , et al . Reconstruction and heterologous expression of a biosynthetic pathway for caffeic acid in Escherichia coli [J ] . Acta Microbiologica Sinica , 2024 , 64 ( 11 ): 4371 - 4387 .

HARTLEY S E , JONES C G , COUPER G C , et al . Biosynthesis of plant phenolic compounds in elevated atmospheric CO 2 [J ] . Global Change Biology , 2000 , 6 ( 5 ): 497 - 506 .

ORTEGA-HERNÁNDEZ E , NAIR V , WELTI-CHANES J , et al . Wounding and UVB light synergistically induce the biosynthesis of phenolic compounds and ascorbic acid in red prickly pears ( Opuntia ficus -indica cv. Rojo vigor) [J ] . International Journal of Molecular Sciences , 2019 , 20 ( 21 ): 5327 .

GULERIA P , KUMAR V . Understanding the phenylpropanoid pathway for agronomical and nutritional improvement of mungbean [J ] . The Journal of Horticultural Science and Biotechnology , 2017 , 92 ( 4 ): 335 - 348 .

SHENDE V V , BAUMAN K D , MOORE B S . The shikimate pathway: gateway to metabolic diversity [J ] . Natural Product Reports , 2024 , 41 ( 4 ): 604 - 648 .

ALMEIDA A M , ABRAHÃO J , SEIXAS F A V , et al . Unraveling shikimate dehydrogenase inhibition by 6-nitroquinazoline-2,4-diol and its impact on soybean and maize growth [J ] . Agronomy , 2024 , 14 ( 5 ): 930 .

YANG L , SUN J Y , ZHANG T Y , et al . Comparative transcriptome analysis and HPLC reveal candidate genes associated with synthesis of bioactive constituents in Rheum palmatum complex [J ] . Physiology and Molecular Biology of Plants , 2024 , 30 ( 8 ): 1239 - 1252 .

BARBER M S , MITCHELL H J . Regulation of phenylpropanoid metabolism in relation to lignin biosynthesis in plants [M ] // International review of cytology . Amsterdam : Elsevier , 1997 : 243 - 293 .

BHAGAT N , MANSOTRA R , PATEL K , et al . Molecular warfare between pathogenic Fusarium oxysporum R1 and host Crocus sativus L. unraveled by dual transcriptomics [J ] . Plant Cell Reports , 2024 , 43 ( 2 ): 42 .

PELED-ZEHAVI H , OLIVA M , XIE Q J , et al . Metabolic engineering of the phenylpropanoid and its primary, precursor pathway to enhance the flavor of fruits and the aroma of flowers [J ] . Bioengineering , 2015 , 2 ( 4 ): 204 - 212 .

JADHAV P R , MAHATMA M K , JHA S , et al . Changes in phenylpropanoid pathway during compatible and incompatible interaction of Ricinus communis - Fusarium oxysporum f.sp. ricini [J ] . Indian Journal of Agricultural Biochemistry , 2013 , 26 ( 1 ): 56 - 60 .

RASHIDIFAR M , ASKARI H , MOGHADAM A . Integrated transcriptomic data reveal a regulatory network for the lignin biosynthesis in Nicotiana tabacum upon drought stress [J ] . Genetic Resources and Crop Evolution , 2025 , 72 ( 3 ): 3581 - 3602 .

YANG J W , PARK S U , LEE H U , et al . Differential responses of antioxidant enzymes and lignin metabolism in susceptible and resistant sweetpotato cultivars during root-knot nematode infection [J ] . Antioxidants , 2023 , 12 ( 6 ): 1164 .

ZHANG Q , WANG S K , QIN B , et al . Analysis of the transcriptome and metabolome reveals phenylpropanoid mechanism in common bean ( Phaseolus vulgaris ) responding to salt stress at sprout stage [J ] . Food and Energy Security , 2023 , 12 ( 5 ): e481 .

TAO X Y , WU Q , LI J Y , et al . Exogenous methyl jasmonate regulates phenolic compounds biosynthesis during postharvest tomato ripening [J ] . Postharvest Biology and Technology , 2022 , 184 : 111760 .

GARCÍA-PÉREZ P , ZHANG L L , MIRAS-MORENO B , et al . The combination of untargeted metabolomics and machine learning predicts the biosynthesis of phenolic compounds in Bryophyllum Medicinal plants (genus Kalanchoe ) [J ] . Plants , 2021 , 10 ( 11 ): 2430 .

DING M L , ZHANG B , ZHANG S , et al . The Sm NPR4- Sm TGA5 module regulates SA-mediated phenolic acid biosynthesis in Salvia miltiorrhiza hairy roots [J ] . Horticulture Research , 2023 , 10 ( 5 ): uhad066 .

CUI J D , LI Y . Response surface optimization of medium composition for phenylalanine ammonia lyase production by recombinant E. coli [C ] // International Conference on Biomass Energy Technologies Proceeding , 2008 , 2 : 6 .

ZHAO Y , CHEN K , YANG H X , et al . Semirational design based on consensus sequences to balance the enzyme activity-stability trade-off [J ] . Journal of Agricultural and Food Chemistry , 2024 , 72 ( 12 ): 6454 - 6462 .

MAO Z J , JIANG H , SUN J N , et al . Research progress in the preparation and structure-activity relationship of bioactive peptides derived from aquatic foods [J ] . Trends in Food Science & Technology , 2024 , 147 : 104443 .

SHI M , HUANG F F , DENG C P , et al . Bioactivities, biosynthesis and biotechnological production of phenolic acids in Salvia miltiorrhiza [J ] . Critical Reviews in Food Science and Nutrition , 2019 , 59 ( 6 ): 953 - 964 .

王炼 . 微生物合成水飞蓟宾体系构建及优化 [D ] . 无锡 : 江南大学 , 2024 .

WANG L . Construction and optimization of microbial biosynthesis system for silybin [D ] . Wuxi : Jiangnan University , 2024 .

周景文 , 陈坚 , 吴俊俊 , 等 . 黄酮类化合物的合成生物学制造 [M ] . 北京 : 化学工业出版社 , 2022 .

ZHOU J W , CHEN J , WU J J . Manufacturing flavonoids by synthetic biology [M ] . Beijing : Chemical Industry Press , 2022 .

ZHANG M M , YUAN B , LI Y Z , et al . Differential global gene transcription of Saccharomyces cerevisiae by zinc sulfate addition under acetic acid stress and identification of novel zinc and stress-responsive genes related to cell wall function [J ] . Fungal Biology , 2025 , 129 ( 4 ): 101573 .

GUO Y L , XIONG Z , ZHAI H T , et al . The advances in creating Crabtree-negative Saccharomyces cerevisiae and the application for chemicals biosynthesis [J ] . FEMS Yeast Research , 2025 , 25 : foaf014 .

FENG J , HAUSER M , COX R J , et al . Engineering Aspergillus oryzae for the heterologous expression of a bacterial modular polyketide synthase [J ] . Journal of Fungi , 2021 , 7 ( 12 ): 1085 .

CAI M M , LIU Z F , ZHAO Z Q , et al . Microbial production of L-methionine and its precursors using systems metabolic engineering [J ] . Biotechnology Advances , 2023 , 69 : 108260 .

NONAKA D , KISHIDA M , HIRATA Y , et al . Modular pathway engineering for enhanced production of para -aminobenzoic acid and 4-amino-phenylalanine in Escherichia coli via glucose/xylose co-utilization [J ] . Applied and Environmental Microbiology , 2025 , 91 ( 5 ): e02468-24 .

KHANDY M T , SOFRONOVA A K , GORPENCHENKO T Y , et al . Plant pyranocoumarins: description, biosynthesis, application [J ] . Plants , 2022 , 11 ( 22 ): 3135 .

LEE K Y , NAM D H , JEON Y , et al . Exploring the production of secondary metabolites from a halophyte Tetragonia tetragonoides through callus culture [J ] . Horticulturae , 2024 , 10 ( 3 ): 244 .

ALARA O R , ABDURAHMAN N H , UKAEGBU C I . Extraction of phenolic compounds: a review [J ] . Current Research in Food Science , 2021 , 4 : 200 - 214 .

IRFAN M , AHMAD T , MONIRUZZAMAN M , et al . Effect of pH on ionic liquid mediated synthesis of gold nanoparticle using elaiseguineensis (palm oil) kernel extract [J ] . IOP Conference Series: Materials Science and Engineering , 2017 , 204 : 012002 .

BAIANO A , DEL NOBILE M A . Antioxidant compounds from vegetable matrices: biosynthesis, occurrence, and extraction systems [J ] . Critical Reviews in Food Science and Nutrition , 2016 , 56 ( 12 ): 2053 - 2068 .

MA J , YANG B , CHEN M , et al . Complete chloroplast genome of Clematis terniflora DC: structural features and gene expression variation induced by UV-B radiation plus incubation in dark [C ] // Proceedings of the 5th Annual Academic Conference of Zhejiang Bioinformatics Society and the International Symposium on Precision Medicine in the Era of Big Data , 2017 : 31 .

王文文 . 发酵豆粕对断奶仔猪肠道屏障的保护作用及机理研究 [D ] . 呼和浩特 : 内蒙古农业大学 , 2023 .

WANG W W . Protective effect and mechanism of fermented soybean meal on gut barrier in weaned piglets [D ] . Hohhot : Inner Mongolia Agricultural University , 2023 .

Functional ingredients from plant resources [C ] // Abstracts of 14th World Congress of Food Science & Technology , 2008 : 36 .

MORTAZAEINEZAHAD F , SAFAVI K . Investigation of epicatechin in barberry fruits [C ] // Proceedings of International Conference on Life Science and Technology . Singapore : LACSIT Press , 2011 , 3 : 156 - 158 .

吴文君 , 胡兆农 , 姬志勤 , 等 . 中国植物源农药研究与应用 [M ] . 北京 : 化学工业出版社 , 2021 .

WU W J , HU Z N , JI Z Q , et al . Research and application of plant-derived pesticides in China [M ] . Beijing : Chemical Industry Press , 2021 .

浏览量

下载量

CSCD

文章被引用时，请邮件提醒。

提交

工具集

关联资源

可控分子量透明质酸的生物合成研究进展

微生物合成二元醇研究进展

真菌源非核糖体肽类药物生物合成及代谢工程

5-氨基乙酰丙酸生物合成技术的发展及展望