核酸生物传感

姚林欣; 宋璐; 李敏; 左小磊

doi:10.12211/2096-8280.2025-015

您当前的位置：

首页 >

文章列表页 >

核酸生物传感

特约评述 | 更新时间：2026-03-07

- 核酸生物传感
- Nucleic acid biosensing
- 合成生物学 2026年7卷第1期页码：66-92
- 作者机构：
  
  上海交通大学医学院附属仁济医院，分子医学研究院，上海 200127
- 作者简介：
  
  姚林欣（2001—），女，硕士研究生。研究方向为框架核酸、临床检验诊断。E-mail：ylx888@sjtu.edu.cn
  左小磊（1980—），男，研究员，博士生导师。研究方向为框架核酸、临床检验诊断、DNA信息存储等。E-mail：zuoxiaolei@sjtu.edu.cn
- 基金信息：
  
  国家重点研发计划(2021YFF1200300;2020YFA0909000);国家自然科学基金(22025404;T2188102);上海市自然科学基金(23ZR1438700;24ZR1445200);上海市高水平大学创新研究团队(SHSMU-ZLCX20212602);上海市卫生健康委员会资助项目(2022JC027);国家资助博士后研究人员计划(GZB20230436)
- DOI：10.12211/2096-8280.2025-015
  中图分类号： Q71
- 收稿：2025-03-13，
  
  修回：2025-04-15，
  
  纸质出版：2026-02-28
- 稿件说明：
移动端阅览
姚林欣，宋璐，李敏，左小磊. 核酸生物传感［J］. 合成生物学， 2026， 7（1）： 66-92

YAO Linxin， SONG Lu， LI Min， ZUO Xiaolei. Nucleic acid biosensing［J］. Synthetic Biology Journal， 2026， 7（1）： 66-92
姚林欣，宋璐，李敏，左小磊. 核酸生物传感［J］. 合成生物学， 2026， 7（1）： 66-92 DOI： 10.12211/2096-8280.2025-015.

YAO Linxin， SONG Lu， LI Min， ZUO Xiaolei. Nucleic acid biosensing［J］. Synthetic Biology Journal， 2026， 7（1）： 66-92 DOI： 10.12211/2096-8280.2025-015.

摘要

脱氧核糖核酸（DNA）分子在生命体内发挥储存和传递遗传信息的生物学功能，在体外，它还可以作为一种可编程的分子自组装纳米材料。基于DNA双螺旋模型和碱基互补配对原则，研究人员开发了多种DNA自组装技术并以此构建了尺寸、形貌可控以及具有动态响应特性的二维和三维纳米结构。鉴于DNA纳米结构具有可控的尺寸、精确的寻址能力、可定制的生物功能、良好的生物相容性等特点，其已被广泛用于生物传感、生物成像、组织工程、药物递送等分子生物学研究领域。本文概述了利用DNA自组装技术构建的二维和三维DNA纳米结构，并分类讨论了DNA链置换驱动以及环境刺激驱动的DNA纳米结构动态变构，重点介绍了DNA纳米结构的生物传感应用，最后展望了基于DNA自组装技术构建的生物传感器的发展前景与面临挑战，包括提高DNA纳米结构的合成效率和稳定性、开发体内动态监测技术、建立多重检测与快速诊断方法以及探索与CRISPR技术联用的新发展方向。

Abstract

Deoxyribonucleic acid (DNA) molecules store and transmit genetic information in all living organisms and some viruses.

In vitro

it can be utilized as a programmable and versatile molecular self-assembling building block to construct functional materials. On the basis of the DNA double helix model and the specific rules of base complementary pairing

where adenine (A) with thymine (T) and cytosine (C) with guanine (G)

researchers have developed various DNA self-assembly techniques over the past few decades

including DNA tiling arrays such as DNA origami and laterally developed single strand tiling. These technologies have been employed to construct a variety of two- or three-dimensional nanoscale structures and devices with controllable sizes and morphologies

as well as dynamic response properties to external environmental stimulus. Researchers have continually demonstrated the exceptional construction capabilities of DNA molecules and have constructed a variety of DNA nanostructures: from simple four-arm nucleic acid junction to origami arrays up to 2～3 microns in size

from two-dimensional planar shapes to three-dimensional complex and twisted structures

and from simple nano-tweezers to command-executing DNA walkers. Due to the unparalleled programmability

precise addressability

editable biological functions

tissue permeability and inherent biocompatibility of DNA nanostructures

they have hold significant potentials in molecular biology research such as biosensing

bioimaging

tissue engineering

and drug delivery. In this review

first

we summarize the construction of two- and three-dimensional DNA nano

structures using various DNA self-assembly technologies. Then

the dynamic transformation of DNA nanostructures driven by two distinct types of driving forces have been categorized and discussed. Finally

the prospects of biosensors based on DNA self-assembly technologies

as well as challenges in this field including enhancing the efficiency and stability of synthesized structures

advancing dynamic monitoring technology in vivo

establishing multiplex and rapid detection methods

and exploring new directions for integration with CRISPR technology

have been explored.

关键词

Keywords

references

SEEMAN N C , KALLENBACH N R . Design of immobile nucleic acid junctions [J ] . Biophysical Journal , 1983 , 44 ( 2 ): 201 - 209 .

FU T J , SEEMAN N C . DNA double-crossover molecules [J ] . Biochemistry , 1993 , 32 ( 13 ): 3211 - 3220 .

ROTHEMUND P W K . Folding DNA to create nanoscale shapes and patterns [J ] . Nature , 2006 , 440 ( 7082 ): 297 - 302 .

WEI B , DAI M J , YIN P . Complex shapes self-assembled from single-stranded DNA tiles [J ] . Nature , 2012 , 485 ( 7400 ): 623 - 626 .

YAO G , ZHANG F , WANG F , et al . Meta-DNA structures [J ] . Nature Chemistry , 2020 , 12 ( 11 ): 1067 - 1075 .

PINHEIRO A V , HAN D R , SHIH W M , et al . Challenges and opportunities for structural DNA nanotechnology [J ] . Nature Nanotechnology , 2011 , 6 ( 12 ): 763 - 772 .

CHEN J , SEEMAN N C . Synthesis from DNA of a molecule with the connectivity of a cube [J ] . Nature , 1991 , 350 ( 6319 ): 631 - 633 .

WINFREE E , LIU F R , WENZLER L A , et al . Design and self-assembly of two-dimensional DNA crystals [J ] . Nature , 1998 , 394 ( 6693 ): 539 - 544 .

HE Y , TIAN Y , CHEN Y , et al . Sequence symmetry as a tool for designing DNA nanostructures [J ] . Angewandte Chemie International Edition , 2005 , 44 ( 41 ): 6694 - 6696 .

ANDERSEN E S , DONG M D , NIELSEN M M , et al . Self-assembly of a nanoscale DNA box with a controllable lid [J ] . Nature , 2009 , 459 ( 7243 ): 73 - 76 .

DOUGLAS S M , DIETZ H , LIEDL T , et al . Self-assembly of DNA into nanoscale three-dimensional shapes [J ] . Nature , 2009 , 459 ( 7245 ): 414 - 418 .

DIETZ H , DOUGLAS S M , SHIH W M . Folding DNA into twisted and curved nanoscale shapes [J ] . Science , 2009 , 325 ( 5941 ): 725 - 730 .

LIU W Y , ZHONG H , WANG R S , et al . Crystalline two-dimensional DNA-origami arrays [J ] . Angewandte Chemie International Edition , 2011 , 50 ( 1 ): 264 - 267 .

ONG L L , HANIKEL N , YAGHI O K , et al . Programmable self-assembly of three-dimensional nanostructures from 10, 000 unique components [J ] . Nature , 2017 , 552 ( 7683 ): 72 - 77 .

WAGENBAUER K F , SIGL C , DIETZ H . Gigadalton-scale shape-programmable DNA assemblies [J ] . Nature , 2017 , 552 ( 7683 ): 78 - 83 .

GE Z L , GU H Z , LI Q , et al . Concept and development of framework nucleic acids [J ] . Journal of the American Chemical Society , 2018 , 140 ( 51 ): 17808 - 17819 .

LIU Y J , DAI Z Z , XIE X D , et al . Spacer-programmed two-dimensional DNA origami assembly [J ] . Journal of the American Chemical Society , 2024 , 146 ( 8 ): 5461 - 5469 .

GEHRING K , LEROY J L , GUÉRON M . A tetrameric DNA structure with protonated cytosine-cytosine base pairs [J ] . Nature , 1993 , 363 ( 6429 ): 561 - 565 .

DHAMODHARAN V , PRADEEPKUMAR P I . Specific recognition of promoter G-quadruplex DNAs by small molecule ligands and light-up probes [J ] . ACS Chemical Biology , 2019 , 14 ( 10 ): 2102 - 2114 .

MA R I , KALLENBACH N R , SHEARDY R D , et al . Three-arm nucleic acid junctions are flexible [J ] . Nucleic Acids Research , 1986 , 14 ( 24 ): 9745 - 9753 .

WANG Y L , MUELLER J E , KEMPER B , et al . Assembly and characterization of five-arm and six-arm DNA branched junctions [J ] . Biochemistry , 1991 , 30 ( 23 ): 5667 - 5674 .

WANG X , SEEMAN N C . Assembly and characterization of 8-arm and 12-arm DNA branched junctions [J ] . Journal of the American Chemical Society , 2007 , 129 ( 26 ): 8169 - 8176 .

ZHANG F , NANGREAVE J , LIU Y , et al . Structural DNA nanotechnology: state of the art and future perspective [J ] . Journal of the American Chemical Society , 2014 , 136 ( 32 ): 11198 - 11211 .

ANDERSEN E S , DONG M D , NIELSEN M M , et al . DNA origami design of dolphin-shaped structures with flexible tails [J ] . ACS Nano , 2008 , 2 ( 6 ): 1213 - 1218 .

HAN D R , PAL S , NANGREAVE J , et al . DNA origami with complex curvatures in three-dimensional space [J ] . Science , 2011 , 332 ( 6027 ): 342 - 346 .

HAN D R , PAL S , YANG Y , et al . DNA gridiron nanostructures based on four-arm junctions [J ] . Science , 2013 , 339 ( 6126 ): 1412 - 1415 .

ZHANG F , JIANG S X , WU S Y , et al . Complex wireframe DNA origami nanostructures with multi-arm junction vertices [J ] . Nature Nanotechnology , 2015 , 10 ( 9 ): 779 - 784 .

TIKHOMIROV G , PETERSEN P , QIAN L L . Fractal assembly of micrometre-scale DNA origami arrays with arbitrary patterns [J ] . Nature , 2017 , 552 ( 7683 ): 67 - 71 .

LABEAN T H , YAN H , KOPATSCH J , et al . Construction, analysis, ligation, and self-assembly of DNA triple crossover complexes [J ] . Journal of the American Chemical Society , 2000 , 122 ( 9 ): 1848 - 1860 .

MATHIEU F , LIAO S P , KOPATSCH J , et al . Six-helix bundles designed from DNA [J ] . Nano Letters , 2005 , 5 ( 4 ): 661 - 665 .

YAN H , PARK S H , FINKELSTEIN G , et al . DNA-templated self-assembly of protein arrays and highly conductive nanowires [J ] . Science , 2003 , 301 ( 5641 ): 1882 - 1884 .

HE Y , CHEN Y , LIU H P , et al . Self-assembly of hexagonal DNA two-dimensional (2D) arrays [J ] . Journal of the American Chemical Society , 2005 , 127 ( 35 ): 12202 - 12203 .

HE Y , TIAN Y , RIBBE A E , et al . Highly connected two-dimensional crystals of DNA six-point-stars [J ] . Journal of the American Chemical Society , 2006 , 128 ( 50 ): 15978 - 15979 .

QIAN L L , WANG Y , ZHANG Z , et al . Analogic China map constructed by DNA [J ] . Chinese Science Bulletin , 2006 , 51 ( 24 ): 2973 - 2976 .

GOODMAN R P , SCHAAP I A T , TARDIN C F , et al . Rapid chiral assembly of rigid DNA building blocks for molecular nanofabrication [J ] . Science , 2005 , 310 ( 5754 ): 1661 - 1665 .

ZHANG Y W , SEEMAN N C . Construction of a DNA-truncated octahedron [J ] . Journal of the American Chemical Society , 1994 , 116 ( 5 ): 1661 - 1669 .

HE Y , YE T , SU M , et al . Hierarchical self-assembly of DNA into symmetric supramolecular polyhedra [J ] . Nature , 2008 , 452 ( 7184 ): 198 - 201 .

LIU X G , ZHANG F , JING X X , et al . Complex silica composite nanomaterials templated with DNA origami [J ] . Nature , 2018 , 559 ( 7715 ): 593 - 598 .

ZHANG C , SU M , HE Y , et al . Conformational flexibility facilitates self-assembly of complex DNA nanostructures [J ] . Proceedings of the National Academy of Sciences of the United States of America , 2008 , 105 ( 31 ): 10665 - 10669 .

LI Y L , TIAN C , LIU Z Y , et al . Structural transformation: assembly of an otherwise inaccessible DNA nanocage [J ] . Angewandte Chemie International Edition , 2015 , 54 ( 20 ): 5990 - 5993 .

BENSON E , MOHAMMED A , GARDELL J , et al . DNA rendering of polyhedral meshes at the nanoscale [J ] . Nature , 2015 , 523 ( 7561 ): 441 - 444 .

IINUMA R , KE Y G , JUNGMANN R , et al . Polyhedra self-assembled from DNA tripods and characterized with 3D DNA-PAINT [J ] . Science , 2014 , 344 ( 6179 ): 65 - 69 .

YURKE B , TURBERFIELD A J , MILLS A P , et al . A DNA-fuelled molecular machine made of DNA [J ] . Nature , 2000 , 406 ( 6796 ): 605 - 608 .

ZHOU C , YANG Z Q , LIU D S . Reversible regulation of protein binding affinity by a DNA machine [J ] . Journal of the American Chemical Society , 2012 , 134 ( 3 ): 1416 - 1418 .

SHIN J S , PIERCE N A . A synthetic DNA walker for molecular transport [J ] . Journal of the American Chemical Society , 2004 , 126 ( 35 ): 10834 - 10835 .

LIU M H , FU J L , HEJESEN C , et al . A DNA tweezer-actuated enzyme nanoreactor [J ] . Nature Communications , 2013 , 4 : 2127 .

KOU B B , CHAI Y Q , YUAN Y L , et al . Dynamical regulation of enzyme cascade amplification by a regenerated DNA nanotweezer for ultrasensitive electrochemical DNA detection [J ] . Analytical Chemistry , 2018 , 90 ( 18 ): 10701 - 10706 .

CHA T G , PAN J , CHEN H R , et al . A synthetic DNA motor that transports nanoparticles along carbon nanotubes [J ] . Nature Nanotechnology , 2014 , 9 ( 1 ): 39 - 43 .

BAZRAFSHAN A , MEYER T A , SU H Q , et al . Tunable DNA origami motors translocate ballistically over μm distances at nm/s speeds [J ] . Angewandte Chemie International Edition , 2020 , 59 ( 24 ): 9514 - 9521 .

CHENG X Y , BAO Y F , LIANG S , et al . Flap endonuclease 1-assisted DNA walkers for sensitively and specifically sensing ctDNAs [J ] . Analytical Chemistry , 2021 , 93 ( 27 ): 9593 - 9601 .

XIN L , ZHOU C , DUAN X Y , et al . A rotary plasmonic nanoclock [J ] . Nature Communications , 2019 , 10 : 5394 .

ZERAATI M , LANGLEY D B , SCHOFIELD P , et al . I-motif DNA structures are formed in the nuclei of human cells [J ] . Nature Chemistry , 2018 , 10 ( 6 ): 631 - 637 .

LIU J B , JING X X , LIU M M , et al . Mechano-fluorescence actuation in single synaptic vesicles with a DNA framework nanomachine [J ] . Science Robotics , 2022 , 7 ( 73 ): eabq5151 .

KUZYK A , YANG Y Y , DUAN X Y , et al . A light-driven three-dimensional plasmonic nanosystem that translates molecular motion into reversible chiroptical function [J ] . Nature Communications , 2016 , 7 : 10591 .

DONG Y C , YANG Z Q , LIU D S . DNA nanotechnology based on i-motif structures [J ] . Accounts of Chemical Research , 2014 , 47 ( 6 ): 1853 - 1860 .

CHEN C , LI M , XING Y Z , et al . Study of pH-induced folding and unfolding kinetics of the DNA i-motif by stopped-flow circular dichroism [J ] . Langmuir , 2012 , 28 ( 51 ): 17743 - 17748 .

UEYAMA H , TAKAGI M , TAKENAKA S . A novel potassium sensing in aqueous media with a synthetic oligonucleotide derivative. Fluorescence resonance energy transfer associated with Guanine quartet-potassium ion complex formation [J ] . Journal of the American Chemical Society , 2002 , 124 ( 48 ): 14286 - 14287 .

HELLER M J . DNA microarray technology: devices, systems, and applications [J ] . Annual Review of Biomedical Engineering , 2002 , 4 : 129 - 153 .

BARTEL D P . MicroRNAs: genomics, biogenesis, mechanism, and function [J ] . Cell , 2004 , 116 ( 2 ): 281 - 297 .

LI M , YIN F F , SONG L , et al . Nucleic acid tests for clinical translation [J ] . Chemical Reviews , 2021 , 121 ( 17 ): 10469 - 10558 .

CHEN Y J , GROVES B , MUSCAT R A , et al . DNA nanotechnology from the test tube to the cell [J ] . Nature Nanotechnology , 2015 , 10 ( 9 ): 748 - 760 .

LIN M H , WEN Y L , LI L Y , et al . Target-responsive, DNA nanostructure-based E-DNA sensor for microRNA analysis [J ] . Analytical Chemistry , 2014 , 86 ( 5 ): 2285 - 2288 .

LIN M H , WANG J J , ZHOU G B , et al . Programmable engineering of a biosensing interface with tetrahedral DNA nanostructures for ultrasensitive DNA detection [J ] . Angewandte Chemie International Edition , 2015 , 54 ( 7 ): 2151 - 2155 .

MIAO P , TANG Y G , YIN J . microRNA detection based on analyte triggered nanoparticle localization on a tetrahedral DNA modified electrode followed by hybridization chain reaction dual amplification [J ] . Chemical Communications , 2015 , 51 ( 86 ): 15629 - 15632 .

LIU L , RONG Q M , KE G L , et al . Efficient and reliable microRNA imaging in living cells via a FRET-based localized hairpin-DNA cascade amplifier [J ] . Analytical Chemistry , 2019 , 91 ( 5 ): 3675 - 3680 .

MENG R , ZHANG X L , LIU J L , et al . Dual-layer 3D DNA nanostructure: the next generation of ultrafast DNA nanomachine for microRNA sensing and intracellular imaging [J ] . Biosensors and Bioelectronics , 2023 , 237 : 115517 .

SUBRAMANIAN H K K , CHAKRABORTY B , SHA R J , et al . The label-free unambiguous detection and symbolic display of single nucleotide polymorphisms on DNA origami [J ] . Nano Letters , 2011 , 11 ( 2 ): 910 - 913 .

WANG X Y , CHEN F , ZHANG D X , et al . Single copy-sensitive electrochemical assay for circulating methylated DNA in clinical samples with ultrahigh specificity based on a sequential discrimination-amplification strategy [J ] . Chemical Science , 2017 , 8 ( 7 ): 4764 - 4770 .

LIU H M , LUO J , FANG L C , et al . An electrochemical strategy with tetrahedron rolling circle amplification for ultrasensitive detection of DNA methylation [J ] . Biosensors & Bioelectronics , 2018 , 121 : 47 - 53 .

WANG S , XIA M C , LIU J , et al . Simultaneous imaging of three tumor-related mRNAs in living cells with a DNA tetrahedron-based multicolor nanoprobe [J ] . ACS Sensors , 2017 , 2 ( 6 ): 735 - 739 .

ZHANG P , LI Z Y , WANG H J , et al . DNA nanomachine-based regenerated sensing platform: a novel electrochemiluminescence resonance energy transfer strategy for ultra-high sensitive detection of microRNA from cancer cells [J ] . Nanoscale , 2017 , 9 ( 6 ): 2310 - 2316 .

QIU X , HILDEBRANDT N . Rapid and multiplexed microRNA diagnostic assay using quantum dot-based Förster resonance energy transfer [J ] . ACS Nano , 2015 , 9 ( 8 ): 8449 - 8457 .

TSANG M K , YE W W , WANG G J , et al . Ultrasensitive detection of Ebola virus oligonucleotide based on upconversion nanoprobe/nanoporous membrane system [J ] . ACS Nano , 2016 , 10 ( 1 ): 598 - 605 .

LIU F , CHOI J Y , SEO T S . Graphene oxide arrays for detecting specific DNA hybridization by fluorescence resonance energy transfer [J ] . Biosensors and Bioelectronics , 2010 , 25 ( 10 ): 2361 - 2365 .

RESCH-GENGER U , GRABOLLE M , CAVALIERE-JARICOT S , et al . Quantum dots versus organic dyes as fluorescent labels [J ] . Nature Methods , 2008 , 5 ( 9 ): 763 - 775 .

SAPSFORD K E , BERTI L , MEDINTZ I L . Materials for fluorescence resonance energy transfer analysis: beyond traditional donor-acceptor combinations [J ] . Angewandte Chemie International Edition , 2006 , 45 ( 28 ): 4562 - 4589 .

LI J M , ZHAO M X , SU H , et al . Multifunctional quantum-dot-based siRNA delivery for HPV18 E6 gene silence and intracellular imaging [J ] . Biomaterials , 2011 , 32 ( 31 ): 7978 - 7987 .

ANDO T . High-speed AFM imaging [J ] . Current Opinion in Structural Biology , 2014 , 28 : 63 - 68 .

FU W Y , ZHANG W . Hybrid AFM for nanoscale physicochemical characterization: recent development and emerging applications [J ] . Small , 2017 , 13 ( 11 ): 1603525 .

LIU K , PAN D , WEN Y Q , et al . Identifying the genotypes of hepatitis B virus (HBV) with DNA origami label [J ] . Small , 2018 , 14 ( 6 ): 1701718 .

ZHANG H L , CHAO J , PAN D , et al . DNA origami-based shape IDs for single-molecule nanomechanical genotyping [J ] . Nature Communications , 2017 , 8 : 14738 .

MUKHERJEE S , GHOSH R N , MAXFIELD F R . Endocytosis [J ] . Physiological Reviews , 1997 , 77 ( 3 ): 759 - 803 .

MONTESANO R , ROTH J , ROBERT A , et al . Non-coated membrane invaginations are involved in binding and internalization of cholera and tetanus toxins [J ] . Nature , 1982 , 296 ( 5858 ): 651 - 653 .

SHI W , CHANCE M R . Metallomics and metalloproteomics [J ] . Cellular and Molecular Life Sciences , 2008 , 65 ( 19 ): 3040 - 3048 .

HUANG Y F , YAN J , FANG Z Z , et al . Highly sensitive and selective optical detection of lead(Ⅱ) using a label-free fluorescent aptasensor [J ] . RSC Advances , 2016 , 6 ( 93 ): 90300 - 90304 .

KOTCH F W , FETTINGER J C , DAVIS J T . A lead-filled G-quadruplex: insight into the G-quartet’s selectivity for Pb 2+ over K + [J ] . Organic Letters , 2000 , 2 ( 21 ): 3277 - 3280 .

LI T , WANG E K , DONG S J . Potassium-lead-switched G-quadruplexes: a new class of DNA logic gates [J ] . Journal of the American Chemical Society , 2009 , 131 ( 42 ): 15082 - 15083 .

ZHONG R B , XIAO M S , ZHU C F , et al . Logic catalytic interconversion of G-molecular hydrogel [J ] . ACS Applied Materials & Interfaces , 2018 , 10 ( 5 ): 4512 - 4518 .

QU X M , YANG F , CHEN H , et al . Bubble-mediated ultrasensitive multiplex detection of metal ions in three-dimensional DNA nanostructure-encoded microchannels [J ] . ACS Applied Materials & Interfaces , 2017 , 9 ( 19 ): 16026 - 16034 .

CHEN B , WANG Y T , MA W J , et al . A mimosa-inspired cell-surface-anchored ratiometric DNA nanosensor for high-resolution and sensitive response of target tumor extracellular pH [J ] . Analytical Chemistry , 2020 , 92 ( 22 ): 15104 - 15111 .

LIU L , DOU C X , LIU J W , et al . Cell surface-anchored DNA nanomachine for dynamically tunable sensing and imaging of extracellular pH [J ] . Analytical Chemistry , 2018 , 90 ( 19 ): 11198 - 11202 .

CHEN J H , ZHOU S G , WEN J L . Disposable strip biosensor for visual detection of Hg 2+ based on Hg 2+ -triggered toehold binding and exonuclease Ⅲ-assisted signal amplification [J ] . Analytical Chemistry , 2014 , 86 ( 6 ): 3108 - 3114 .

LANZ E , GREGOR M , SLAVÍK J , et al . Use of FITC as a fluorescent probe for intracellular pH measurement [J ] . Journal of Fluorescence , 1997 , 7 ( 4 ): 317 - 319 .

MORDON S , DEVOISSELLE J M , MAUNOURY V . In vivo pH measurement and imaging of tumor tissue using a pH-sensitive fluorescent probe (5,6-carboxyfluorescein): instrumental and experimental studies [J ] . Photochemistry and Photobiology , 1994 , 60 ( 3 ): 274 - 279 .

BAE W , YOON T Y , JEONG C . Direct evaluation of self-quenching behavior of fluorophores at high concentrations using an evanescent field [J ] . PLoS One , 2021 , 16 ( 2 ): e0247326 .

CHEN G , LIU D , HE C B , et al . Enzymatic synthesis of periodic DNA nanoribbons for intracellular pH sensing and gene silencing [J ] . Journal of the American Chemical Society , 2015 , 137 ( 11 ): 3844 - 3851 .

LI N , WANG M M , GAO X N , et al . A DNA tetrahedron nanoprobe with controlled distance of dyes for multiple detection in living cells and in vivo [J ] . Analytical Chemistry , 2017 , 89 ( 12 ): 6670 - 6677 .

ZHAO J , LI Y H , YU M M , et al . Time-resolved activation of pH sensing and imaging in vivo by a remotely controllable DNA nanomachine [J ] . Nano Letters , 2020 , 20 ( 2 ): 874 - 880 .

CARDONE R A , CASAVOLA V , RESHKIN S J . The role of disturbed pH dynamics and the Na + /H + exchanger in metastasis [J ] . Nature Reviews Cancer , 2005 , 5 ( 10 ): 786 - 795 .

ANDERSON M , MOSHNIKOVA A , ENGELMAN D M , et al . Probe for the measurement of cell surface pH in vivo and ex vivo [J ] . Proceedings of the National Academy of Sciences of the United States of America , 2016 , 113 ( 29 ): 8177 - 8181 .

SAHA S , CHAKRABORTY K , KRISHNAN Y . Tunable, colorimetric DNA-based pH sensors mediated by a-motif formation [J ] . Chemical Communications , 2012 , 48 ( 19 ): 2513 - 2515 .

RICH A , DAVIES D R , CRICK F H C , et al . The molecular structure of polyadenylic acid [J ] . Journal of Molecular Biology , 1961 , 3 ( 1 ): 71 -IN19.

FRESCO J R . Polynucleotides Ⅱ: the X-ray diffraction patterns of solutions of the randomly coiled and helical forms of polyriboadenylic acid [J ] . Journal of Molecular Biology , 1959 , 1 ( 2 ): 106 -IN3.

TS’O P O P , HELMKAMP G K , SANDER C . Interaction of nucleosides and related compounds with nucleic acids as indicated by the change of helix-coil transition temperature [J ] . Proceedings of the National Academy of Sciences of the United States of America , 1962 , 48 ( 4 ): 686 - 698 .

LI X X , LIU Y M , LI Y J , et al . High glucose concentration induces endothelial cell proliferation by regulating cyclin-D2-related miR-98 [J ] . Journal of Cellular and Molecular Medicine , 2016 , 20 ( 6 ): 1159 - 1169 .

SANG F M , ZHANG X , LIU J , et al . A label-free hairpin aptamer probe for colorimetric detection of adenosine triphosphate based on the anti-aggregation of gold nanoparticles [J ] . Spectrochimica Acta Part A, Molecular and Biomolecular Spectroscopy , 2019 , 217 : 122 - 127 .

SHENG Q L , LIU R X , ZHANG S , et al . Ultrasensitive electrochemical cocaine biosensor based on reversible DNA nanostructure [J ] . Biosensors and Bioelectronics , 2014 , 51 : 191 - 194 .

ZHU Z , WU C C , LIU H P , et al . An aptamer cross-linked hydrogel as a colorimetric platform for visual detection [J ] . Angewandte Chemie International Edition , 2010 , 49 ( 6 ): 1052 - 1056 .

WALTER H K , BAUER J , STEINMEYER J , et al . “DNA origami traffic lights” with a split aptamer sensor for a bicolor fluorescence readout [J ] . Nano Letters , 2017 , 17 ( 4 ): 2467 - 2472 .

XIONG Z W , WANG Q , XIE Y J , et al . Simultaneous detection of aflatoxin B 1 and ochratoxin A in food samples by dual DNA tweezers nanomachine [J ] . Food Chemistry , 2021 , 338 : 128122 .

YANG H H , LIU H P , KANG H Z , et al . Engineering target-responsive hydrogels based on aptamer-target interactions [J ] . Journal of the American Chemical Society , 2008 , 130 ( 20 ): 6320 - 6321 .

MA Y L , MAO Y , AN Y , et al . Target-responsive DNA hydrogel for non-enzymatic and visual detection of glucose [J ] . Analyst , 2018 , 143 ( 7 ): 1679 - 1684 .

MENG H M , ZHANG X B , LÜ Y F , et al . DNA dendrimer: an efficient nanocarrier of functional nucleic acids for intracellular molecular sensing [J ] . ACS Nano , 2014 , 8 ( 6 ): 6171 - 6181 .

WANG Y H , SONG W , ZHAO H Y , et al . DNA walker-assisted aptasensor for highly sensitive determination of Ochratoxin A [J ] . Biosensors and Bioelectronics , 2021 , 182 : 113171 .

PIN J P , BETTLER B . Organization and functions of mGlu and GABAB receptor complexes [J ] . Nature , 2016 , 540 ( 7631 ):　 60 - 68 .

JENKINS W J , PETERS T J . Mitochondrial enzyme activities in liver biopsies from patients with alcoholic liver disease [J ] . Gut , 1978 , 19 ( 5 ): 341 - 344 .

YAN H , ZHONG G C , XU G W , et al . Sodium taurocholate cotransporting polypeptide is a functional receptor for human hepatitis B and D virus [J ] . eLife , 2012 , 1 : e00049 .

LI J R , WANG J , GREWAL Y S , et al . Multiplexed SERS detection of soluble cancer protein biomarkers with gold-silver alloy nanoboxes and nanoyeast single-chain variable fragments [J ] . Analytical Chemistry , 2018 , 90 ( 17 ): 10377 - 10384 .

HORI S S , GAMBHIR S S . Mathematical model identifies blood biomarker-based early cancer detection strategies and limitations [J ] . Science Translational Medicine , 2011 , 3 ( 109 ): 109ra116 .

CARDOZO K H M , LEBKUCHEN A , OKAI G G , et al . Establishing a mass spectrometry-based system for rapid detection of SARS-CoV-2 in large clinical sample cohorts [J ] . Nature Communications , 2020 , 11 : 6201 .

ZHOU X Y , ZHAO M , DUAN X L , et al . Collapse of DNA tetrahedron nanostructure for “off-on” fluorescence detection of DNA methyltransferase activity [J ] . ACS Applied Materials & Interfaces , 2017 , 9 ( 46 ): 40087 - 40093 .

DAEMS D , PFEIFER W , RUTTEN I , et al . Three-dimensional DNA origami as programmable anchoring points for bioreceptors in fiber optic surface plasmon resonance biosensing [J ] . ACS Applied Materials & Interfaces , 2018 , 10 ( 28 ): 23539 - 23547 .

YAN J , HU C Y , WANG P , et al . Novel rolling circle amplification and DNA origami-based DNA belt-involved signal amplification assay for highly sensitive detection of prostate-specific antigen (PSA) [J ] . ACS Applied Materials & Interfaces , 2014 , 6 ( 22 ): 20372 - 20377 .

RAVEENDRAN M , LEE A J , SHARMA R , et al . Rational design of DNA nanostructures for single molecule biosensing [J ] . Nature Communications , 2020 , 11 : 4384 .

FENG Q M , MA P , CAO Q H , et al . An aptamer-binding DNA walking machine for sensitive electrochemiluminescence detection of tumor exosomes [J ] . Chemical Communications , 2020 , 56 ( 2 ): 269 - 272 .

HOU J , LI X T , XIE K P . Coupled liquid biopsy and bioinformatics for pancreatic cancer early detection and precision prognostication [J ] . Molecular Cancer , 2021 , 20 ( 1 ): 34 .

KARAMI P , KHOSHSAFAR H , JOHARI-AHAR M , et al . Colorimetric immunosensor for determination of prostate specific antigen using surface plasmon resonance band of colloidal triangular shape gold nanoparticles [J ] . Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy , 2019 , 222 : 117218 .

SELL S . AFP as a marker for liver cell injury: differentiation of tumor growth, hepatotoxicity, and carcinogenesis [J ] . UCLA Forum in Medical Sciences , 1978 , 20 : 51 - 58 .

GHORBANI F , ABBASZADEH H , DOLATABADI J E N , et al . Application of various optical and electrochemical aptasensors for detection of human prostate specific antigen: a review [J ] . Biosensors and Bioelectronics , 2019 , 142 : 111484 .

SUN Y L , FAN J F , CUI L Y , et al . Fluorometric nanoprobes for simultaneous aptamer-based detection of carcinoembryonic antigen and prostate specific antigen [J ] . Microchimica Acta , 2019 , 186 ( 3 ): 152 .

CHEN C T , FENG S L , ZHOU M , et al . Development of a structure-switching aptamer-based nanosensor for salicylic acid detection [J ] . Biosensors and Bioelectronics , 2019 , 140 : 111342 .

TAGHDISI S M , DANESH N M , NAMEGHI M A , et al . A DNA triangular prism-based fluorescent aptasensor for ultrasensitive detection of prostate-specific antigen [J ] . Analytica Chimica Acta , 2020 , 1120 : 36 - 42 .

AHMADI-SANGACHIN E , MOHAMMADNEJAD J , HOSSEINI M . Fluorescence self-assembled DNA hydrogel for the determination of prostate specific antigen by aggregation induced emission [J ] . Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy , 2023 , 303 : 123234 .

QIN Y , LI D X , YUAN R , et al . Netlike hybridization chain reaction assembly of DNA nanostructures enables exceptional signal amplification for sensing trace cytokines [J ] . Nanoscale , 2019 , 11 ( 35 ): 16362 - 16367 .

URABE F , KOSAKA N , ITO K , et al . Extracellular vesicles as biomarkers and therapeutic targets for cancer [J ] . American Journal of Physiology Cell Physiology , 2020 , 318 ( 1 ): C29 - C39 .

THÉRY C , ZITVOGEL L , AMIGORENA S . Exosomes: composition, biogenesis and function [J ] . Nature Reviews Immunology , 2002 , 2 ( 8 ): 569 - 579 .

WANG C C , JIN D , YU Y , et al . A dual antibody-modified nanochannel biosensor for capture and identification of exosomes [J ] . Sensors and Actuators B: Chemical , 2020 , 314 : 128056 .

ZHOU Q , RAHIMIAN A , SON K , et al . Development of an aptasensor for electrochemical detection of exosomes [J ] . Methods , 2016 , 97 : 88 - 93 .

ZHANG H X , WANG Z H , ZHANG Q X , et al . Ti 3 C 2 MXenes nanosheets catalyzed highly efficient electrogenerated chemiluminescence biosensor for the detection of exosomes [J ] . Biosensors and Bioelectronics , 2019 , 124-125 : 184 - 190 .

ZHAO L , SUN R J , HE P , et al . Ultrasensitive detection of exosomes by target-triggered three-dimensional DNA walking machine and exonuclease Ⅲ-assisted electrochemical ratiometric biosensing [J ] . Analytical Chemistry , 2019 , 91 ( 22 ): 14773 - 14779 .

NAGRATH S , SEQUIST L V , MAHESWARAN S , et al . Isolation of rare circulating tumour cells in cancer patients by microchip technology [J ] . Nature , 2007 , 450 ( 7173 ): 1235 - 1239 .

YOON H J , KIM T H , ZHANG Z , et al . Sensitive capture of circulating tumour cells by functionalized graphene oxide nanosheets [J ] . Nature Nanotechnology , 2013 , 8 ( 10 ): 735 - 741 .

ZHAO W A , CUI C H , BOSE S , et al . Bioinspired multivalent DNA network for capture and release of cells [J ] . Proceedings of the National Academy of Sciences of the United States of America , 2012 , 109 ( 48 ): 19626 - 19631 .

ZHOU G B , LIN M H , SONG P , et al . Multivalent capture and detection of cancer cells with DNA nanostructured biosensors and multibranched hybridization chain reaction amplification [J ] . Analytical Chemistry , 2014 , 86 ( 15 ): 7843 - 7848 .

SONG P , YE D K , ZUO X L , et al . DNA hydrogel with aptamer-toehold-based recognition, cloaking, and decloaking of circulating tumor cells for live cell analysis [J ] . Nano Letters , 2017 , 17 ( 9 ): 5193 - 5198 .

WANG J , DONG H Y , ZHOU Y Y , et al . Immunomagnetic antibody plus aptamer pseudo-DNA nanocatenane followed by rolling circle amplication for highly-sensitive CTC detection [J ] . Biosensors and Bioelectronics , 2018 , 122 : 239 - 246 .

XIONG J R , DONG C , ZHANG J J , et al . DNA walker-powered ratiometric SERS cytosensor of circulating tumor cells with single-cell sensitivity [J ] . Biosensors and Bioelectronics , 2022 , 213 : 114442 .

MORRIS J G , ACHESON D . Cholera and other types of vibriosis: a story of human pandemics and oysters on the half shell [J ] . Clinical Infectious Diseases , 2003 , 37 ( 2 ): 272 - 280 .

BISHARA J , LEIBOVICI L , GARTMAN-ISRAEL D , et al . Long-term outcome of infective endocarditis: the impact of early surgical intervention [J ] . Clinical Infectious Diseases , 2001 , 33 ( 10 ): 1636 - 1643 .

MUSHER D M , THORNER A R . Community-acquired pneumonia [J ] . The New England Journal of Medicine , 2014 , 371 ( 17 ): 1619 - 1628 .

GIOVANNI M , SETYAWATI M I , TAY C Y , et al . Electrochemical quantification of Escherichia coli with DNA nanostructure [J ] . Advanced Functional Materials , 2015 , 25 ( 25 ): 3840 - 3846 .

WANG J P , LEONG M C , LEONG E Z W , et al . Clinically relevant detection of Streptococcus pneumoniae with DNA-antibody nanostructures [J ] . Analytical Chemistry , 2017 , 89 ( 12 ): 6900 - 6906 .

ZHANG X Y , SUN R M , ZHENG H R , et al . Amplification-free sensitive detection of Staphylococcus aureus by spherical nucleic acid triggered CRISPR/Cas12a and poly T-Cu reporter [J ] . Microchimica Acta , 2025 , 192 ( 2 ): 76 .

YU J L , WU H H , HE L Y , et al . The universal dual-mode aptasensor for simultaneous determination of different bacteria based on naked eyes and microfluidic-chip together with magnetic DNA encoded probes [J ] . Talanta , 2021 , 225 : 122062 .

XIAO M S , ZOU K , LI L , et al . Stochastic DNA walkers in droplets for super-multiplexed bacterial phenotype detection [J ] . Angewandte Chemie International Edition , 2019 , 58 ( 43 ): 15448 - 15454 .

WEI Y D , TAO Z Z , WAN L , et al . Aptamer-based Cas14a1 biosensor for amplification-free live pathogenic detection [J ] . Biosensors and Bioelectronics , 2022 , 211 : 114282 .

SUN Y H , DUAN N , MA P F , et al . Colorimetric aptasensor based on truncated aptamer and trivalent DNAzyme for Vibrio parahemolyticus determination [J ] . Journal of Agricultural and Food Chemistry , 2019 , 67 ( 8 ): 2313 - 2320 .

YANG H H , XIAO M S , LAI W , et al . Stochastic DNA dual-walkers for ultrafast colorimetric bacteria detection [J ] . Analytical Chemistry , 2020 , 92 ( 7 ): 4990 - 4995 .

BROWN T M , FAKIH H H , SALIBA D , et al . Stabilization of functional DNA structures with mild photochemical methods [J ] . Journal of the American Chemical Society , 2023 , 145 ( 4 ): 2142 - 2151 .

BAI T X , ZHANG J Y , HUANG K , et al . Reconfiguration of DNA nanostructures induced by enzymatic ligation treatment [J ] . Nucleic Acids Research , 2022 , 50 ( 14 ): 8392 - 8398 .

HANG X M , LIU P F , TIAN S , et al . Rapid and sensitive detection of Ebola RNA in an unamplified sample based on CRISPR-Cas13a and DNA roller machine [J ] . Biosensors and Bioelectronics , 2022 , 211 : 114393 .

YAO S , LIU Y , DING Y K , et al . Three-dimensional DNA nanomachine biosensor coupled with CRISPR Cas12a cascade amplification for ultrasensitive detection of carcinoembryonic antigen [J ] . Journal of Nanobiotechnology , 2024 , 22 ( 1 ): 266 .

GUO J R , ZHU Y L , MIAO P . Nano-impact electrochemical biosensing based on a CRISPR-responsive DNA hydrogel [J ] . Nano Letters , 2023 , 23 ( 23 ): 11099 - 11104 .

浏览量

279

下载量

CSCD

文章被引用时，请邮件提醒。

提交

工具集

关联资源

单细胞转录组联合生物传感——宫颈癌分子诊断方法研发新框架

N-糖链合成的研究进展

基于合成生物学的唾液酸乳糖合成方法及其应用研究进展

基于体外合成生物学的健康糖生物制造新技术与产业化进展

糖质ivBT生物合成的核心元件及关键技术