植物合成生物学：植物细胞大规模培养的新机遇

颜钊涛; 周鹏飞; 汪阳忠; 张鑫; 谢雯燕; 田晨菲; 王勇

doi:10.12211/2096-8280.2024-095

您当前的位置：

首页 >

文章列表页 >

植物合成生物学：植物细胞大规模培养的新机遇

特约评述 | 更新时间：2025-11-12

- 植物合成生物学：植物细胞大规模培养的新机遇
- Plant synthetic biology: new opportunities for large-scale culture of plant cells
- 合成生物学 2025年6卷第5期页码：1107-1125
- 作者机构：
  
  1.上海烟草集团有限责任公司烟草行业卷烟烟气重点实验室，上海 201315
  2.中国科学院分子植物科学卓越创新中心，植物生理生态研究所，中国科学院合成生物学重点实验室，上海 200032
  3.中国科学院大学，北京 100049
  4.广东医科大学基础医学院，广东东莞 523808
- 作者简介：
  
  [ "颜钊涛（1998—），男，硕士研究生。主要研究方向为植物合成生物学及植物细胞培养。E-mail：yanzhaotao@cemps.ac.cn" ]
  [ "田晨菲（1995—），女，博士后。研究方向为植物合成生物学。E-mail：tianchenfei@cemps.ac.cn" ]
  [ "王勇（1974—），男，研究员，博士生导师。研究方向为天然产物合成生物学，通过解析天然产物的生物合成途径，运用合成生物学的思想和方法，基于工程化的设计和建构，改进复杂天然产物的生物合成效率和其生产方式，开发天然的或非天然的复杂天然产物活性成分。E-mail：yongwang@cemps.ac.cn" ]
- 基金信息：
  
  国家重点研发计划(SQ2024YFA1700126);上海市市级重大专项(24HC2820400);上海烟草集团有限责任公司行业卷烟烟气重点实验室开放性课题(K2023-1-044P)
- DOI：10.12211/2096-8280.2024-095
  中图分类号： Q81
- 收稿：2024-12-18，
  
  修回：2025-02-24，
  
  纸质出版：2025-10-31
- 稿件说明：
移动端阅览
颜钊涛，周鹏飞，汪阳忠，张鑫，谢雯燕，田晨菲，王勇. 植物合成生物学：植物细胞大规模培养的新机遇［J］. 合成生物学， 2025， 6（5）： 1107-1125

YAN Zhaotao， ZHOU Pengfei， WANG Yangzhong， ZHANG Xin， XIE Wenyan， TIAN Chenfei， WANG Yong. Plant synthetic biology： new opportunities for large-scale culture of plant cells［J］. Synthetic Biology Journal， 2025， 6（5）： 1107-1125
颜钊涛，周鹏飞，汪阳忠，张鑫，谢雯燕，田晨菲，王勇. 植物合成生物学：植物细胞大规模培养的新机遇［J］. 合成生物学， 2025， 6（5）： 1107-1125 DOI： 10.12211/2096-8280.2024-095.

YAN Zhaotao， ZHOU Pengfei， WANG Yangzhong， ZHANG Xin， XIE Wenyan， TIAN Chenfei， WANG Yong. Plant synthetic biology： new opportunities for large-scale culture of plant cells［J］. Synthetic Biology Journal， 2025， 6（5）： 1107-1125 DOI： 10.12211/2096-8280.2024-095.

摘要

植物细胞培养（plant cell culture， PCC）作为一种极具发展潜力的生物合成平台，具有生长周期短、成本效益高、无病原危害、次生代谢产物丰富等优势，在医药、食品和保健等领域备受关注。然而，生产效率不足是限制PCC应用于商业化生产的最大阻碍，其中，遗传转化效率低、调控网络复杂、细胞结团及遗传稳定性差是主要困难。合成生物学遵循自下而上的工程化建造理念，对天然植物细胞进行精准设计与改造，为开发高效、经济可行的植物细胞工厂提供了新的解决方案。本文回顾了PCC作为合成平台在生产重组蛋白和次生代谢产物中的研究现状。重点探讨了植物合成生物学对PCC在工业化发展中的推动作用，包括优质植物细胞系的构建、遗传转化体系的优化、表达系统的优化、生产效率与产能的提升以及赋予植物细胞合成异源产物的能力。未来，PCC的发展更需强调合成生物学理念和技术在突破当前技术瓶颈中的关键作用，以促进植物细胞大规模培养的进一步发展。

Abstract

Plant Cell Culture (PCC) has emerged as a highly promising chassis for synthetic biology

offering a range of advantages such as short growth cycles

cost-effectiveness

absence of pathogenic risks

and abundant secondary metabolites. These features make PCC an attractive alternative for applications in medicine

food

and health. However

insufficient production efficiency due to difficulties in genetic transformation

complex regulatory networks

cell aggregation

and poor genetic stability remains a major obstacle that limits the commercialization of PCC. Synthetic biology

with its bottom-up engineering design approach

provides a powerful toolkit to address these challenges. By enabling the precise design and modification of native plant cells

synthetic biology offers innovative strategies to develop efficient and economically viable plant cell factories. In this paper

we first review the current status of PCC in synthesizing high-value compounds

particularly recombinant proteins and secondary metabolites. Recent advancements have demonstrated the potential of PCC to produce therapeutic proteins

vaccines

industrial enzymes and bioactive compounds such as alkaloids

flavonoids

and terpenoids. These successes underscore the versatility of PCC as a bioproduction platform. We then explore the role of synthetic biology in advancing PCC industrialization. Key developments include the creation of high-quality plant cell lines through genome editing tools like CRISPR/Cas9

enhancing genetic stability and metabolic efficiency. Additionally

synthetic biology has improved genetic transformation systems

overcoming a critical bottleneck in PCC. Enhanced expression systems

incorporating synthetic promoters and regulatory elements

have significantly boosted target compound yields. Furthermore

synthetic biology has expanded PCC applications by enabling the biosynthesis of heterologous compounds beyond their native metabolic pathways. Finally

we discuss future prospects

emphasizing the potential of synthetic biology to overcome current technical challenges. Emerging technologies including multi-omics integration

machine learning

and synthetic organelle development are anticipated to further enhance PCC’s scalability and efficiency. By addressing these challenges

synthetic biology will pave the way for large-scale plant cell cultivation

thereby facilitating its widespread adoption in industrial bioproduction. The convergence of PCC and synthetic biology holds immense potential for the sustainable

cost-effective

and scalable production of high-value compounds.

关键词

Keywords

references

MURTHY H N , LEE E J , PAEK K Y . Production of secondary metabolites from cell and organ cultures: strategies and approaches for biomass improvement and metabolite accumulation [J ] . Plant Cell, Tissue and Organ Culture (PCTOC) , 2014 , 118 ( 1 ): 1 - 16 .

NAGATA T , KUMAGAI F . Plant cell biology through the window of the highly synchronized tobacco BY-2 cell line [J ] . Methods in Cell Science , 1999 , 21 ( 2 ): 123 - 127 .

RAHMAN Z A , AHMAD SEMAN Z , OTHMAN A N , et al . Efficient callus induction and plant regeneration of Malaysian indica rice MR219 using anther culture [J ] . Biocatalysis and Agricultural Biotechnology , 2021 , 31 : 101865 .

LI Y L , HUANG S W , ZHANG J Y , et al . A protocol of homozygous haploid callus induction from endosperm of Taxus chinensis Rehd. var. mairei [J ] . SpringerPlus , 2016 , 5 ( 1 ): 659 .

OBAE S G , KLANDORF H , WEST T P . Growth characteristics and ginsenosides production of in vitro tissues of American ginseng, Panax quinquefolius L [J ] . HortScience , 2011 , 46 ( 8 ): 1136 - 1140 .

LE V , SUKHIKH A , LARICHEV T , et al . Isolation of the main biologically active substances and phytochemical analysis of Ginkgo biloba callus culture extracts [J ] . Molecules , 2023 , 28 ( 4 ): 1560 .

EIBL R , MEIER P , STUTZ I , et al . Plant cell culture technology in the cosmetics and food industries: current state and future trends [J ] . Applied Microbiology and Biotechnology , 2018 , 102 ( 20 ): 8661 - 8675 .

EMMANUEL D B , ISHAKU G A , ANDREW F P , et al . Callus culture for the production of therapeutic compounds [J ] . American Journal of Plant Biology , 2019 , 4 ( 4 ): 76 .

NETT R S , LAU W , SATTELY E S . Discovery and engineering of colchicine alkaloid biosynthesis [J ] . Nature , 2020 , 584 ( 7819 ): 148 - 153 .

MAEDA H A . Harnessing evolutionary diversification of primary metabolism for plant synthetic biology [J ] . Journal of Biological Chemistry , 2019 , 294 ( 45 ): 16549 - 16566 .

MEYER H P , SCHMIDHALTER D R . Industrial scale suspension culture of living cells [M/OL ] . Weinheim , Germany : Wiley Blackwell , 2014 . ( 2014-06-18 )[ 2024-12-01 ] . https://onlinelibrary.wiley.com/doi/book/10.1002/9783527683321 https://onlinelibrary.wiley.com/doi/book/10.1002/9783527683321 .

DIRISALA V R , NAIR R R , SRIRAMA K , et al . Recombinant pharmaceutical protein production in plants: unraveling the therapeutic potential of molecular pharming [J ] . Acta Physiologiae Plantarum , 2016 , 39 ( 1 ): 18 .

ARYA S S , ROOKES J E , CAHILL D M , et al . Next-generation metabolic engineering approaches towards development of plant cell suspension cultures as specialized metabolite producing biofactories [J ] . Biotechnology Advances , 2020 , 45 : 107635 .

HUSSAIN M S , FAREED S , ANSARI S , et al . Current approaches toward production of secondary plant metabolites [J ] . Journal of Pharmacy & Bioallied Sciences , 2012 , 4 ( 1 ): 10 - 20 .

RAMIREZ-ESTRADA K , VIDAL-LIMON H , HIDALGO D , et al . Elicitation, an effective strategy for the biotechnological production of bioactive high-added value compounds in plant cell factories [J ] . Molecules , 2016 , 21 ( 2 ): 182 .

NAGEGOWDA D A , GUPTA P . Advances in biosynthesis, regulation, and metabolic engineering of plant specialized terpenoids [J ] . Plant Science , 2020 , 294 : 110457 .

FONSECA-SANTOS B , CORRÊA M A , CHORILLI M . Sustainability, natural and organic cosmetics: consumer, products, efficacy, toxicological and regulatory considerations [J ] . Brazilian Journal of Pharmaceutical Sciences , 2015 , 51 ( 1 ): 17 - 26 .

GOMORD V , FAYE L . Posttranslational modification of therapeutic proteins in plants [J ] . Current Opinion in Plant Biology , 2004 , 7 ( 2 ): 171 - 181 .

GHAG S B , ADKI V S , GANAPATHI T R , et al . Plant platforms for efficient heterologous protein production [J ] . Biotechnology and Bioprocess Engineering , 2021 , 26 ( 4 ): 546 - 567 .

SINGH A A , PILLAY P , TSEKOA T L . Engineering approaches in plant molecular farming for global health [J ] . Vaccines , 2021 , 9 ( 11 ): 1270 .

WU J P , ZHANG J X , HAO X Y , et al . Establishment of an efficient callus transient transformation system for Vitis vinifera cv. ‘Chardonnay’ [J ] . Protoplasma , 2024 , 261 ( 2 ): 351 - 366 .

CORTESE E , CARRARETTO L , BALDAN B , et al . Arabidopsis photosynthetic and heterotrophic cell suspension cultures [J ] . Methods in Molecular Biology , 2021 , 2200 : 167 - 185 .

LI B , TAKAHASHI D , KAWAMURA Y , et al . Plasma membrane proteome analyses of Arabidopsis thaliana suspension-cultured cells during cold or ABA treatment: relationship with freezing tolerance and growth phase [J ] . Journal of Proteomics , 2020 , 211 : 103528 .

SEGEČOVÁ A , ČERVENÝ J , ROITSCH T . Advancement of the cultivation and upscaling of photoautotrophic suspension cultures using Chenopodium rubrum as a case study [J ] . Plant Cell, Tissue and Organ Culture (PCTOC) , 2018 , 135 ( 1 ): 37 - 51 .

THORPE T A . History of plant tissue culture [J ] . Molecular Biotechnology , 2007 , 37 ( 2 ): 169 - 180 .

SRBA M , ČERNÍKOVÁ A , OPATRNÝ Z , et al . Practical guidelines for the characterization of tobacco BY-2 cell lines [J ] . Biologia Plantarum , 2016 , 60 ( 1 ): 13 - 24 .

IKEDA N , KAMIMURA M , UESUGI K , et al . Choline chloride and N -allylglycine promote plant growth by increasing the efficiency of photosynthesis [J ] . Bioscience, Biotechnology, and Biochemistry , 2024 , 89 ( 1 ): 51 - 61 .

LEE E K , JIN Y W , PARK J H , et al . Cultured cambial meristematic cells as a source of plant natural products [J ] . Nature Biotechnology , 2010 , 28 ( 11 ): 1213 - 1217 .

ZHOU P F , LI H H , LIN Y J , et al . Omics analyses of Rehmannia glutinosa dedifferentiated and cambial meristematic cells reveal mechanisms of catalpol and indole alkaloid biosynthesis [J ] . BMC Plant Biology , 2023 , 23 ( 1 ): 463 .

PATRA N , SRIVASTAVA A K . Artemisinin production by plant hairy root cultures in gas- and liquid-phase bioreactors [J ] . Plant Cell Reports , 2016 , 35 ( 1 ): 143 - 153 .

HA L T , PAWLICKI-JULLIAN N , PILLON-LEQUART M , et al . Hairy root cultures of Panax vietnamensis , a promising approach for the production of ocotillol-type ginsenosides [J ] . Plant Cell, Tissue and Organ Culture (PCTOC) , 2016 , 126 ( 1 ): 93 - 103 .

PERASSOLO M , CARDILLO A B , MUGAS M L , et al . Enhancement of anthraquinone production and release by combination of culture medium selection and methyl jasmonate elicitation in hairy root cultures of Rubia tinctorum [J ] . Industrial Crops and Products , 2017 , 105 : 124 - 132 .

NGUYEN T M , WU P Y , CHANG C H , et al . High-yield BMP2 expression in rice cells via CRISPR and endogenous αAmy3 promoter [J ] . Applied Microbiology and Biotechnology , 2024 , 108 ( 1 ): 206 .

KIM T G , BAEK M Y , LEE E K , et al . Expression of human growth hormone in transgenic rice cell suspension culture [J ] . Plant Cell Reports , 2008 , 27 ( 5 ): 885 - 891 .

KIM N S , YU H Y , CHUNG N D , et al . Production of functional recombinant bovine trypsin in transgenic rice cell suspension cultures [J ] . Protein Expression and Purification , 2011 , 76 ( 1 ): 121 - 126 .

JUNG J W , KIM N S , JANG S H , et al . Production and characterization of recombinant human acid α-glucosidase in transgenic rice cell suspension culture [J ] . Journal of Biotechnology , 2016 , 226 : 44 - 53 .

CHUNG N D , KIM N S , VAN GIAP D , et al . Production of functional human vascular endothelial growth factor 165 in transgenic rice cell suspension cultures [J ] . Enzyme and Microbial Technology , 2014 , 63 : 58 - 63 .

KIM T G , KIM M Y , TIEN N Q D , et al . Dengue virus E glycoprotein production in transgenic rice callus [J ] . Molecular Biotechnology , 2014 , 56 ( 12 ): 1069 - 1078 .

CHEN L , YANG X Y , LUO D , et al . Efficient production of a bioactive bevacizumab monoclonal antibody using the 2A self-cleavage peptide in transgenic rice callus [J ] . Frontiers in Plant Science , 2016 , 7 : 1156 .

ZHANG N N , WRIGHT T , CARAWAY P , et al . Enhanced secretion of human α1-antitrypsin expressed with a novel glycosylation module in tobacco BY-2 cell culture [J ] . Bioengineered , 2019 , 10 ( 1 ): 87 - 97 .

XU J F , OKADA S , TAN L , et al . Human growth hormone expressed in tobacco cells as an Arabinogalactan-protein fusion glycoprotein has a prolonged serum life [J ] . Transgenic Research , 2010 , 19 ( 5 ): 849 - 867 .

GENGENBACH B B , KEIL L L , OPDENSTEINEN P , et al . Comparison of microbial and transient expression (tobacco plants and plant-cell packs) for the production and purification of the anticancer mistletoe lectin viscumin [J ] . Biotechnology and Bioengineering , 2019 , 116 ( 9 ): 2236 - 2249 .

RATNER M . Genzyme resumes shipping as Sanofi-aventis hovers [J ] . Nature Biotechnology , 2010 , 28 ( 10 ): 994 .

MIHALIAK C A , FANTON M J , MCMILLEN J K . Preparation of vaccine master cell lines using recombinant plant suspension cultures : US2006/041305 [P ] . 2010-01-14 .

HANANIA U , ARIEL T , TEKOAH Y , et al . Establishment of a tobacco BY2 cell line devoid of plant-specific xylose and fucose as a platform for the production of biotherapeutic proteins [J ] . Plant Biotechnology Journal , 2017 , 15 ( 9 ): 1120 - 1129 .

HUANG T K , MCDONALD K A . Bioreactor systems for in vitro production of foreign proteins using plant cell cultures [J ] . Biotechnology Advances , 2012 , 30 ( 2 ): 398 - 409 .

ALCALDE M A , PEREZ-MATAS E , ESCRICH A , et al . Biotic elicitors in adventitious and hairy root cultures: a review from 2010 to 2022 [J ] . Molecules , 2022 , 27 ( 16 ): 5253 .

CHODISETTI B , RAO K , GANDI S , et al . Improved gymnemic acid production in the suspension cultures of Gymnema sylvestre through biotic elicitation [J ] . Plant Biotechnology Reports , 2013 , 7 ( 4 ): 519 - 525 .

LIANG C X , CHEN C , ZHOU P F , et al . Effect of Aspergillus flavus fungal elicitor on the production of terpenoid indole alkaloids in Catharanthus roseus cambial meristematic cells [J ] . Molecules , 2018 , 23 ( 12 ): 3276 .

NAMDEO A G . Plant cell elicitation for production of secondary metabolites: a review [J ] . Pharmacognosy Reviews , 2007 , 1 ( 1 ): 69 - 79 .

AN Y , FENG S , XU Y , et al . ChemInform abstract: hydrothermal synthesis and characterization of a new potassium phosphatoantimonate, K 8 Sb 8 P 2 O 29 ×8 H 2 O [J ] . ChemInform , 1996 , 27 ( 21 ): 199621020 .

EFFERTH T . Biotechnology applications of plant callus cultures [J ] . Engineering , 2019 , 5 ( 1 ): 50 - 59 .

ZHANG Z J , SUN Y H , LI Y . Plant rejuvenation: from phenotypes to mechanisms [J ] . Plant Cell Reports , 2020 , 39 ( 10 ): 1249 - 1262 .

TAKEDA T , MIZUKAMI M , MATSUOKA H . Characterization of two-step direct somatic embryogenesis in carrot [J ] . Biochemical Engineering Journal , 2008 , 38 ( 2 ): 206 - 211 .

LU Y , LIU Z Y , LYU M L , et al . Characterization of JsWOX1 and JsWOX4 during callus and root induction in the shrub species Jasminum sambac [J ] . Plants , 2019 , 8 ( 4 ): 79 .

FENG M , ZHANG A , NGUYEN V , et al . A conserved graft formation process in Norway spruce and Arabidopsis identifies the PAT gene family as central regulators of wound healing [J ] . Nature Plants , 2024 , 10 ( 1 ): 53 - 65 .

CAO H F , ZHANG X , LI F , et al . Glucosinolate O -methyltransferase mediated callus formation and affected ROS homeostasis in Arabidopsis thaliana [J ] . Physiology and Molecular Biology of Plants , 2024 , 30 ( 1 ): 109 - 121 .

SU Y H , TANG L P , ZHAO X Y , et al . Plant cell totipotency: insights into cellular reprogramming [J ] . Journal of Integrative Plant Biology , 2021 , 63 ( 1 ): 228 - 243 .

XU C Y , CHANG P J , GUO S Q , et al . Transcriptional activation by WRKY23 and derepression by removal of bHLH041 coordinately establish callus pluripotency in Arabidopsis regeneration [J ] . The Plant Cell , 2023 , 36 ( 1 ): 158 - 173 .

YANG W T , ZHAI H W , WU F M , et al . Peptide REF1 is a local wound signal promoting plant regeneration [J ] . Cell , 2024 , 187 ( 12 ): 3024 - 3038.e14 .

张博 , 马永硕 , 尚轶 , 等 . 植物合成生物学研究进展 [J ] . 合成生物学 , 2020 , 1 ( 2 ): 121 - 140 .

ZHANG B , MA Y S , SHANG Y , et al . Recent advances in plant synthetic biology [J ] . Synthetic Biology Journal , 2020 , 1 ( 2 ): 121 - 140 .

YOOSEFZADEH-NAJAFABADI M , TORABI S , TULPAN D , et al . Genome-wide association studies of soybean yield-related hyperspectral reflectance bands using machine learning-mediated data integration methods [J ] . Frontiers in Plant Science , 2021 , 12 : 777028 .

RAMEZANPOUR M R , FARAJPOUR M . Application of artificial neural networks and genetic algorithm to predict and optimize greenhouse banana fruit yield through nitrogen, potassium and magnesium [J ] . PLoS One , 2022 , 17 ( 2 ): e0264040 .

HESAMI M , ALIZADEH M , JONES A M P , et al . Machine learning: its challenges and opportunities in plant system biology [J ] . Applied Microbiology and Biotechnology , 2022 , 106 ( 9-10 ): 3507 - 3530 .

JAFARI M , DANESHVAR M H . Prediction and optimization of indirect shoot regeneration of Passiflora caerulea using machine learning and optimization algorithms [J ] . BMC Biotechnology , 2023 , 23 ( 1 ): 27 .

FALLAH ZIARANI M , TOHIDFAR M , NAVVABI M . Modeling and optimizing in vitro percentage and speed callus induction of carrot via Multilayer Perceptron-Single point discrete GA and radial basis function [J ] . BMC Biotechnology , 2022 , 22 ( 1 ): 34 .

REZAEI H , MIRZAIE-ASL A , ABDOLLAHI M R , et al . Enhancing Petunia tissue culture efficiency with machine learning: a pathway to improved callogenesis [J ] . PLoS One , 2023 , 18 ( 11 ): e0293754 .

GRIGOREVA E I , SIDORCHUK Y V , DEINEKO E V . Aggregates’ formation in higher plants’ cell culture: the role of cell wall components [J ] . Biology Bulletin Reviews , 2022 , 12 ( 2 ): S182 - S194 .

KOLEWE M E , HENSON M A , ROBERTS S C . Analysis of aggregate size as a process variable affecting paclitaxel accumulation in Taxus suspension cultures [J ] . Biotechnology Progress , 2011 , 27 ( 5 ): 1365 - 1372 .

PATIL R A , KOLEWE M E , ROBERTS S C . Cellular aggregation is a key parameter associated with long term variability in paclitaxel accumulation in Taxus suspension cultures [J ] . Plant Cell, Tissue and Organ Culture , 2013 , 112 ( 3 ): 303 - 310 .

NYON M P , DU L Y , TSENG C K , et al . Engineering a stable CHO cell line for the expression of a MERS-coronavirus vaccine antigen [J ] . Vaccine , 2018 , 36 ( 14 ): 1853 - 1862 .

BISSINGER T , WU Y X , MARICHAL-GALLARDO P , et al . Towards integrated production of an influenza A vaccine candidate with MDCK suspension cells [J ] . Biotechnology and Bioengineering , 2021 , 118 ( 10 ): 3996 - 4013 .

SHEN C F , GUILBAULT C , LI X L , et al . Development of suspension adapted Vero cell culture process technology for production of viral vaccines [J ] . Vaccine , 2019 , 37 ( 47 ): 6996 - 7002 .

LEE N , SHIN J , PARK J H , et al . Targeted gene deletion using DNA-free RNA-guided Cas9 nuclease accelerates adaptation of CHO cells to suspension culture [J ] . ACS Synthetic Biology , 2016 , 5 ( 11 ): 1211 - 1219 .

MOHNEN D . Pectin structure and biosynthesis [J ] . Current Opinion in Plant Biology , 2008 , 11 ( 3 ): 266 - 277 .

YEH B J , RUTIGLIANO R J , DEB A , et al . Rewiring cellular morphology pathways with synthetic guanine nucleotide exchange factors [J ] . Nature , 2007 , 447 ( 7144 ): 596 - 600 .

GLEBA Y Y , TUSÉ D , GIRITCH A . Plant viral vectors for delivery by Agrobacterium [M/OL ] //PALMER K, GLEBA Y. Plant viral vectors . Berlin, Heidelberg: Springer Berlin Heidelberg, 2013 , 375 : 155 - 192 . ( 2013-08-15)[2024-12-14] . https://link.springer.com/10.1007/82_2013_352 https://link.springer.com/10.1007/82_2013_352 .

HESAMI M , ALIZADEH M , NADERI R , et al . Forecasting and optimizing Agrobacterium -mediated genetic transformation via ensemble model-fruit fly optimization algorithm: a data mining approach using chrysanthemum databases [J ] . PloS One , 2020 , 15 ( 9 ); e0239901 .

JIANG T , ZHANG Y , ZUO G G , et al . Transcription factor PgNAC72 activates DAMMARENEDIOL SYNTHASE expression to promote ginseng saponin biosynthesis [J ] . Plant Physiology , 2024 , 195 ( 4 ): 2952 - 2969 .

ZHAO Y , CHENG P , LIU Y , et al . A highly efficient soybean transformation system using GRF3-GIF1 chimeric protein [J ] . Journal of Integrative Plant Biology , 2025 , 67 ( 1 ): 3 - 6 .

JHA P , KUMAR V . BABY BOOM ( BBM ): a candidate transcription factor gene in plant biotechnology [J ] . Biotechnology Letters , 2018 , 40 ( 11 ): 1467 - 1475 .

LOWE K , WU E , WANG N , et al . Morphogenic regulators baby boom and wuschel improve monocot transformation [J ] . The Plant Cell , 2016 , 28 ( 9 ): 1998 - 2015 .

KOBERCOVÁ E , SRBA M , FISCHER L . Sulfadiazine and phosphinothricin selection systems optimised for the transformation of tobacco BY-2 cells [J ] . Plant Cell Reports , 2023 , 42 ( 3 ): 535 - 548 .

HE Y B , ZHANG T , SUN H , et al . A reporter for noninvasively monitoring gene expression and plant transformation [J ] . Horticulture Research , 2020 , 7 : 152 .

TIAN C F , ZHANG Y X , LI J H , et al . Benchmarking intrinsic promoters and terminators for plant synthetic biology research [J ] . Biodesign Research , 2022 , 2022 : 9834989 .

YAMASAKI S , SUZUKI A , YAMANO Y , et al . Identification of 5 ' -untranslated regions that function as effective translational enhancers in monocotyledonous plant cells using a novel method of genome-wide analysis [J ] . Plant Biotechnology , 2018 , 35 ( 4 ): 365 - 373 .

HUANG L F , TAN C C , YEH J F , et al . Efficient secretion of recombinant proteins from rice suspension-cultured cells modulated by the choice of signal peptide [J ] . PLoS One , 2015 , 10 ( 10 ): e0140812 .

WOJCIK S , KRIECHBAUMER V . Go your own way: membrane-targeting sequences [J ] . Plant Physiology , 2021 , 185 ( 3 ): 608 - 618 .

SELMA S , SANMARTÍN N , ESPINOSA-RUIZ A , et al . Custom-made design of metabolite composition in N. benthamiana leaves using CRISPR activators [J ] . Plant Biotechnology Journal , 2022 , 20 ( 8 ): 1578 - 1590 .

PEREZ-MATAS E , HIDALGO-MARTINEZ D , MOYANO E , et al . Overexpression of BAPT and DBTNBT genes in Taxus baccata in vitro cultures to enhance the biotechnological production of paclitaxel [J ] . Plant Biotechnology Journal , 2024 , 22 ( 1 ): 233 - 247 .

ZUO A Q , HE D , SUN C R , et al . Integration of induction, system optimization and genetic transformation in Veratrum californicum var. vitro cultures to enhance the production of cyclopamine and veratramine [J ] . Plant Physiology and Biochemistry , 2024 , 216 : 109087 .

CHU M Y , PEDREÑO M A , ALBURQUERQUE N , et al . A new strategy to enhance the biosynthesis of trans-resveratrol by overexpressing stilbene synthase gene in elicited Vitis vinifera cell cultures [J ] . Plant Physiology and Biochemistry , 2017 , 113 : 141 - 148 .

LI J H , MUTANDA I , WANG K B , et al . Chloroplastic metabolic engineering coupled with isoprenoid pool enhancement for committed taxanes biosynthesis in Nicotiana benthamiana [J ] . Nature Communications , 2019 , 10 : 4850 .

SHI Y S , WANG D , LI R S , et al . Engineering yeast subcellular compartments for increased production of the lipophilic natural products ginsenosides [J ] . Metabolic Engineering , 2021 , 67 : 104 - 111 .

KARLSON C K S , MOHD NOOR S N , KHALID N , et al . CRISPRi-mediated down-regulation of the cinnamate-4-hydroxylase (C4H) gene enhances the flavonoid biosynthesis in Nicotiana tabacum [J ] . Biology , 2022 , 11 ( 8 ): 1127 .

ZHANG W H , ZHANG J Z , FAN Y D , et al . RNA sequencing analysis reveals PgbHLH28 as the key regulator in response to methyl jasmonate-induced saponin accumulation in Platycodon grandiflorus [J ] . Horticulture Research , 2024 , 11 ( 5 ): uhae058 .

KARPPINEN K , LAFFERTY D J , ALBERT N W , et al . MYBA and MYBPA transcription factors co-regulate anthocyanin biosynthesis in blue-coloured berries [J ] . New Phytologist , 2021 , 232 ( 3 ): 1350 - 1367 .

APPELHAGEN I , WULFF-VESTER A K , WENDELL M , et al . Colour bio-factories: towards scale-up production of anthocyanins in plant cell cultures [J ] . Metabolic Engineering , 2018 , 48 : 218 - 232 .

JIA E T , LI H , HE F , et al . Metabolic engineering of artificially modi fied transcription factor SmMYB36-VP16 for high-level production of tanshinones and phenolic acids [J ] . Metabolic Engineering , 2024 , 86 : 29 - 40 .

GE C , YU Z , SHENG H K , et al . Redesigning regulatory components of quorum-sensing system for diverse metabolic control [J ] . Nature Communications , 2022 , 13 : 2182 .

WU J J , BAO M J , DUAN X G , et al . Developing a pathway-independent and full-autonomous global resource allocation strategy to dynamically switching phenotypic states [J ] . Nature Communications , 2020 , 11 : 5521 .

KURZBACH E , STRIEKER M , WITTSTOCK U . Production of benzylglucosinolate in genetically engineered carrot suspension cultures [J ] . Plant Biotechnology , 2022 , 39 ( 3 ): 241 - 250 .

VASILEV N , SCHMITZ C , GRÖMPING U , et al . Assessment of cultivation factors that affect biomass and geraniol production in transgenic tobacco cell suspension cultures [J ] . PLoS One , 2014 , 9 ( 8 ): e104620 .

CHUN J H , ADHIKARI P B , PARK S B , et al . Production of the dammarene sapogenin (protopanaxadiol) in transgenic tobacco plants and cultured cells by heterologous expression of PgDDS and CYP716A47 [J ] . Plant Cell Reports , 2015 , 34 ( 9 ): 1551 - 1560 .

MURATA J , MATSUMOTO E , MORIMOTO K , et al . Generation of triple-transgenic Forsythia cell cultures as a platform for the efficient, stable, and sustainable production of lignans [J ] . PLoS One , 2015 , 10 ( 12 ): e0144519 .

LU S W , ZHANG Y , ZHU K J , et al . The Citrus transcription factor CsMADS6 modulates carotenoid metabolism by directly regulating carotenogenic genes [J ] . Plant Physiology , 2018 , 176 ( 4 ): 2657 - 2676 .

DONG Y S , DUAN W L , HE H X , et al . Enhancing taxane biosynthesis in cell suspension culture of Taxus chinensis by overexpressing the neutral/alkaline invertase gene [J ] . Process Biochemistry , 2015 , 50 ( 4 ): 651 - 660 .

CHEE M J Y , LYCETT G W , KHOO T J , et al . Bioengineering of the plant culture of Capsicum frutescens with vanillin synthase gene for the production of vanillin [J ] . Molecular Biotechnology , 2017 , 59 ( 1 ): 1 - 8 .

HUSAIN Z , WARSI Z I , KHAN S , et al . Metabolic engineering of hairy root cultures in beta vulgaris for enhanced production of vanillin, 4-hydroxybenzoic acid, and vanillyl alcohol [J ] . Frontiers in Bioengineering and Biotechnology , 2024 , 12 : 1435190 .

MAYER M J , NARBAD A , PARR A J , et al . Rerouting the plant phenylpropanoid pathway by expression of a novel bacterial enoyl-CoA hydratase/lyase enzyme function [J ] . The Plant Cell , 2001 , 13 ( 7 ): 1669 - 1682 .

KITAOKA N , NOMURA T , OGITA S , et al . Bioproduction of glucose conjugates of 4-hydroxybenzoic and vanillic acids using bamboo cells transformed to express bacterial 4-hydroxycinnamoyl-CoA hydratase/lyase [J ] . Journal of Bioscience and Bioengineering , 2020 , 130 ( 1 ): 89 - 97 .

IMAMURA T , ISOZUMI N , HIGASHIMURA Y , et al . Isolation of amaranthin synthetase from Chenopodium quinoa and construction of an amaranthin production system using suspension-cultured tobacco BY-2 cells [J ] . Plant Biotechnology Journal , 2019 , 17 ( 5 ): 969 - 981 .

HAN J Y , WANG H Y , CHOI Y E . Production of dammarenediol-Ⅱ triterpene in a cell suspension culture of transgenic tobacco [J ] . Plant Cell Reports , 2014 , 33 ( 2 ): 225 - 233 .

SOBRINO-MENGUAL G , ALVAREZ D , TWYMAN R M , et al . Activation of the native PHYTOENE SYNTHASE 1 promoter by modifying near-miss cis -acting elements induces carotenoid biosynthesis in embryogenic rice callus [J ] . Plant Cell Reports , 2024 , 43 ( 5 ): 118 .

HIDALGO D , GEORGIEV M , MARCHEV A , et al . Tailoring tobacco hairy root metabolism for the production of stilbenes [J ] . Scientific Reports , 2017 , 7 : 17976 .

DEUS-NEUMANN B , ZENK M H . Instability of indole alkaloid production in Catharanthus roseus cell suspension cultures [J ] . Planta Medica , 1984 , 50 ( 5 ): 427 - 431 .

ISHCHUK O P , DOMENZAIN I , SÁNCHEZ B J , et al . Genome-scale modeling drives 70-fold improvement of intracellular heme production in Saccharomyces cerevisiae [J ] . Proceedings of the National Academy of Sciences of the United States of America , 2022 , 119 ( 30 ): e2108245119 .

CRISTIANA GOMES DE OLIVEIRA DAL’MOLIN L Q . AraGEM, a genome-scale reconstruction of the primary metabolic network in Arabidopsis [J ] . Plant Physiology , 2010 , 152 ( 2 ): 579 - 589 .

MURALI S , IBRAHIM M , RAJENDRAN H , et al . Genome-scale metabolic model led engineering of Nothapodytes nimmoniana plant cells for high camptothecin production [J ] . Frontiers in Plant Science , 2023 , 14 : 1207218 .

LAKSHMANAN M , LIM S H , MOHANTY B , et al . Unraveling the light-specific metabolic and regulatory signatures of rice through combined in silico modeling and multiomics analysis [J ] . Plant Physiology , 2015 , 169 ( 4 ): 3002 - 3020 .

CHOWDHURY N B , SIMONS-SENFTLE M , DECOUARD B , et al . A multi-organ maize metabolic model connects temperature stress with energy production and reducing power generation [J ] . iScience , 2023 , 26 ( 12 ): 108400 .

YUAN H L , MAURICE CHEUNG C Y , POOLMAN M G , et al . A genome-scale metabolic network reconstruction of tomato ( Solanum lycopersicum L.) and its application to photorespiratory metabolism [J ] . The Plant Journal , 2016 , 85 ( 2 ): 289 - 304 .

CUNHA E , SILVA M , CHAVES I , et al . The first multi-tissue genome-scale metabolic model of a woody plant highlights suberin biosynthesis pathways in Quercus suber [J ] . PLoS Computational Biology , 2023 , 19 ( 9 ): e1011499 .

ARANO-VARELA H , FERNÁNDEZ F J , ESTRADA-ZÚÑIGA M E , et al . Verbascoside production in long-term Buddleja cordata Kunth cell suspension cultures [J ] . 3 Biotech , 2020 , 10 ( 6 ): 245 .

TREJO-TAPIA G , BALCAZAR-AGUILAR J B , MARTÍNEZ-BONFIL B , et al . Effect of screening and subculture on the production of betaxanthins in Beta vulgaris L. var. ‘Dark Detroit’ callus culture [J ] . Innovative Food Science & Emerging Technologies , 2008 , 9 ( 1 ): 32 - 36 .

COPPEDE J S , PINA E S , PAZ T A , et al . Cell cultures of Maytenus ilicifolia Mart. are richer sources of quinone-methide triterpenoids than plant roots in natura [J ] . Plant Cell, Tissue and Organ Culture (PCTOC) , 2014 , 118 ( 1 ): 33 - 43 .

ZHU X F , MOHSIN A , ZAMAN W Q , et al . Development of a novel noninvasive quantitative method to monitor Siraitia grosvenorii cell growth and browning degree using an integrated computer-aided vision technology and machine learning [J ] . Biotechnology and Bioengineering , 2021 , 118 ( 10 ): 4092 - 4104 .

浏览量

下载量

CSCD

文章被引用时，请邮件提醒。

提交

工具集

关联资源

天然产物的化学-酶法合成：方法与策略的演进

面向碳达峰与碳中和的植物合成生物学