作物光合作用合成生物学的策略与展望

孙扬; 陈立超; 石艳云; 王珂; 吕丹丹; 徐秀美; 张立新

doi:10.12211/2096-8280.2024-094

您当前的位置：

首页 >

文章列表页 >

作物光合作用合成生物学的策略与展望

特约评述 | 更新时间：2025-11-12

- 作物光合作用合成生物学的策略与展望
- Strategies and prospects of synthetic biology in crop photosynthesis
- 合成生物学 2025年6卷第5期页码：1025-1040
- 作者机构：
  
  河南大学生命科学学院，省部共建作物逆境适应与改良国家重点实验室，河南省合成生物与生物制造重点实验室，河南开封 475004
- 作者简介：
  
  [ "孙扬（1993—），男，讲师，硕士生导师。研究方向为逆境下光合作用调控机制以及光合作用合成生物学。E-mail：sunyy@henu.edu.cn" ]
  [ "张立新（1970—），男，教授，博士生导师。研究方向为光合作用功能调控机理，包括叶绿体基因表达调控、光合膜复合物组装、叶绿体信号转导、光合作用环境调节等。 E-mail：zhanglixin@henu.edu.cn" ]
- 基金信息：
  
  国家重点研发计划“合成生物学”重点专项(2020YFA0907600;2023YFA0914601);河南省中原学者项目(234000510005)
- DOI：10.12211/2096-8280.2024-094
  中图分类号： Q81
- 收稿：2024-12-17，
  
  修回：2025-03-12，
  
  纸质出版：2025-10-31
- 稿件说明：
移动端阅览
孙扬，陈立超，石艳云，王珂，吕丹丹，徐秀美，张立新. 作物光合作用合成生物学的策略与展望［J］. 合成生物学， 2025， 6（5）： 1025-1040

SUN Yang， CHEN Lichao， SHI Yanyun， WANG Ke， LV Dandan， XU Xiumei， ZHANG Lixin. Strategies and prospects of synthetic biology in crop photosynthesis［J］. Synthetic Biology Journal， 2025， 6（5）： 1025-1040
孙扬，陈立超，石艳云，王珂，吕丹丹，徐秀美，张立新. 作物光合作用合成生物学的策略与展望［J］. 合成生物学， 2025， 6（5）： 1025-1040 DOI： 10.12211/2096-8280.2024-094.

SUN Yang， CHEN Lichao， SHI Yanyun， WANG Ke， LV Dandan， XU Xiumei， ZHANG Lixin. Strategies and prospects of synthetic biology in crop photosynthesis［J］. Synthetic Biology Journal， 2025， 6（5）： 1025-1040 DOI： 10.12211/2096-8280.2024-094.

摘要

光合作用是地球上几乎所有生命活动的能量和物质来源，其效率直接影响作物的生长和产量。随着合成生物学的快速发展，研究者们开始探索通过工程化手段，从不同层次优化光合作用的基本环节，包括光能利用、碳固定、光呼吸及光合逆境适应等。本文综述了近年来在提高光合作用效率方面的研究进展，重点讨论了新型光能转化模型的构建、Rubisco的定向进化与活性改造、碳同化途径的优化、光呼吸支路的设计以及逆境高光效回路的构建等策略。通过合成生物学的手段，可以显著提高植物的光合效率和抗逆能力，实现生物量和作物产量的提升，为应对全球粮食安全挑战提供新的解决方案。未来，基于合成生物学的策略，深入解析光合作用的分子机制，结合人工智能等新兴技术，将为光合作用的工程化改造提供更为有效的方法和途径，实现作物光合作用效率的显著提升。

Abstract

Photosynthesis is the primary source of energy and materials for nearly all life activities on Earth

and its efficiency directly impacts crop growth and yield. With the rapid development of synthetic biology

researchers have begun to explore engineering approaches to optimize the fundamental processes of photosynthesis at various levels

including light energy utilization

carbon fixation

photorespiration

and stress adaptation. This review summarizes recent advances in improving photosynthetic efficiency

with a focus on the synthetic biological strategies that can be im

plemented in crops. To achieve efficient light absorption and electron transport

novel light energy conversion models have been developed

involving the engineering of light-harvesting antennae to minimize energy loss and the development of orthogonal electron transport chains to enhance quantum yield. Multi-level optimization strategies have been developed for carbon assimilation pathways

including directed evolution and activity modification of Rubisco

optimization of key enzymes in the Calvin-Benson-Bassham cycle

and the introduction of CO

concentrating mechanisms into C

plants. Furthermore

novel photorespiratory bypasses have been engineered through synthetic biology approaches

which optimize glycolate metabolism to effectively reduce photorespiratory carbon loss while enhancing photosynthetic efficiency in crops. Additionally

various engineering strategies have been developed to optimize photosynthetic performance under adverse conditions

such as the enhancement of non-photochemical quenching components to tolerate high light and the application of stress-responsive elements to adapt to temperature fluctuations. By employing synthetic biology techniques

significant improvements in plant photosynthetic efficiency and stress resistance have been achieved. This has led to enhanced biomass and crop yields

thereby providing new solutions to address global food security challenges. In the future

strategies based on synthetic biology

combined with a deeper understanding of the molecular mechanisms of photosynthesis and emerging technologies like artificial intelligence

will offer more effective methods and pathways for the engineering of photosynthesis

resulting in a substantial enhancement of crop photosynthetic efficiency.

关键词

Keywords

references

LEISTER D . Genetic engineering, synthetic biology and the light reactions of photosynthesis [J ] . Plant Physiology , 2019 , 179 ( 3 ): 778 - 793 .

LONG S P , AINSWORTH E A , LEAKEY A D B , et al . Global food insecurity. Treatment of major food crops with elevated carbon dioxide or ozone under large-scale fully open-air conditions suggests recent models may have overestimated future yields [J ] . Philosophical Transactions of the Royal Society of London Series B , Biological Sciences, 2005 , 360 ( 1463 ): 2011 - 2020 .

张立新 , 卢从明 , 彭连伟 , 等 . 利用合成生物学原理提高光合作用效率的研究进展 [J ] . 生物工程学报 , 2017 , 33 ( 3 ): 486 - 493 .

ZHANG L X , LU C M , PENG L W , et al . Progress in improving photosynthetic efficiency by synthetic biology [J ] . Chinese Journal of Biotechnology , 2017 , 33 ( 3 ): 486 - 493 .

朱新广 , 熊燕 , 阮梅花 , 等 . 光合作用合成生物学研究现状及未来发展策略 [J ] . 中国科学院院刊 , 2018 , 33 ( 11 ): 1239 - 1248 .

ZHU X G , XIONG Y , RUAN M H , et al . Research status and future development strategies of synthetic biology in photosynthesis [J ] . Bulletin of Chinese Academy of Sciences , 2018 , 33 ( 11 ): 1239 - 1248 .

DRUBIN D A , WAY J C , SILVER P A . Designing biological systems [J ] . Genes & Development , 2007 , 21 ( 3 ): 242 - 254 .

ZHAO Y , COELHO C , HUGHES A L , et al . Debugging and consolidating multiple synthetic chromosomes reveals combinatorial genetic interactions [J ] . Cell , 2023 , 186 ( 24 ): 5220 - 5236.e16 .

SCHINDLER D , WALKER R S K , JIANG S Y , et al . Design, construction, and functional characterization of a tRNA neochromosome in yeast [J ] . Cell , 2023 , 186 ( 24 ): 5237 - 5253.e22 .

ZHANG W M , LAZAR-STEFANITA L , YAMASHITA H , et al . Manipulating the 3D organization of the largest synthetic yeast chromosome [J ] . Molecular Cell , 2023 , 83 ( 23 ): 4424 - 4437.e5 .

HANANIA U , ARIEL T , TEKOAH Y , et al . Establishment of a tobacco BY2 cell line devoid of plant-specific xylose and fucose as a platform for the production of biotherapeutic proteins [J ] . Plant Biotechnology Journal , 2017 , 15 ( 9 ): 1120 - 1129 .

ČERMÁK T , CURTIN S J , GIL-HUMANES J , et al . A multipurpose toolkit to enable advanced genome engineering in plants [J ] . The Plant Cell , 2017 , 29 ( 6 ): 1196 - 1217 .

LEISTER D . Enhancing the light reactions of photosynthesis: strategies, controversies, and perspectives [J ] . Molecular Plant , 2023 , 16 ( 1 ): 4 - 22 .

BAG P , CHUKHUTSINA V , ZHANG Z S , et al . Direct energy transfer from photosystem Ⅱ to photosystem Ⅰ confers winter sustainability in Scots pine [J ] . Nature Communications , 2020 , 11 : 6388 .

ORT D R , MERCHANT S S , ALRIC J , et al . Redesigning photosynthesis to sustainably meet global food and bioenergy demand [J ] . Proceedings of the National Academy of Sciences of the United States of America , 2015 , 112 ( 28 ): 8529 - 8536 .

CHEN M , BLANKENSHIP R E . Expanding the solar spectrum used by photosynthesis [J ] . Trends in Plant Science , 2011 , 16 ( 8 ): 427 - 431 .

ROCHAIX J D . Regulation and dynamics of the light-harvesting system [J ] . Annual Review of Plant Biology , 2014 , 65 : 287 - 309 .

NÜRNBERG D J , MORTON J , SANTABARBARA S , et al . Photochemistry beyond the red limit in chlorophyll f-containing photosystems [J ] . Science , 2018 , 360 ( 6394 ): 1210 - 1213 .

CHEN M , SCHLIEP M , WILLOWS R D , et al . A red-shifted chlorophyll [J ] . Science , 2010 , 329 ( 5997 ): 1318 - 1319 .

KATO K , SHINODA T , NAGAO R , et al . Structural basis for the adaptation and function of chlorophyll f in photosystem Ⅰ [J ] . Nature Communications , 2020 , 11 : 238 .

BLANKENSHIP R E , TIEDE D M , BARBER J , et al . Comparing photosynthetic and photovoltaic efficiencies and recognizing the potential for improvement [J ] . Science , 2011 , 332 ( 6031 ): 805 - 809 .

ORT D R , ZHU X G , MELIS A . Optimizing antenna size to maximize photosynthetic efficiency [J ] . Plant Physiology , 2011 , 155 ( 1 ): 79 - 85 .

TIAN J G , WANG C L , CHEN F Y , et al . Maize smart-canopy architecture enhances yield at high densities [J ] . Nature , 2024 , 632 ( 8025 ): 576 - 584 .

YAMAMOTO H , TAKAHASHI S , BADGER M R , et al . Artificial remodelling of alternative electron flow by flavodiiron proteins in Arabidopsis [J ] . Nature Plants , 2016 , 2 : 16012 .

DANN M , LEISTER D . Evidence that cyanobacterial Sll1217 functions analogously to PGRL1 in enhancing PGR5-dependent cyclic electron flow [J ] . Nature Communications , 2019 , 10 : 5299 .

PESARESI P , SCHARFENBERG M , WEIGEL M , et al . Mutants, overexpressors, and interactors of Arabidopsis plastocyanin isoforms: revised roles of plastocyanin in photosynthetic electron flow and thylakoid redox state [J ] . Molecular Plant , 2009 , 2 ( 2 ): 236 - 248 .

SIMKIN A J , MCAUSLAND L , LAWSON T , et al . Overexpression of the RieskeFeS protein increases electron transport rates and biomass yield [J ] . Plant Physiology , 2017 , 175 ( 1 ): 134 - 145 .

ERMAKOVA M , LOPEZ-CALCAGNO P E , RAINES C A , et al . Overexpression of the Rieske FeS protein of the cytochrome b6f complex increases C 4 photosynthesis in Setaria viridis [J ] . Communications Biology , 2019 , 2 : 314 .

HEYNO E , ERMAKOVA M , LOPEZ-CALCAGNO P E , et al . Rieske FeS overexpression in tobacco provides increased abundance and activity of cytochrome b6f [J ] . Physiologia Plantarum , 2022 , 174 ( 6 ): e13803 .

CHEN J H , CHEN S T , HE N Y , et al . Nuclear-encoded synthesis of the D1 subunit of photosystem Ⅱ increases photosynthetic efficiency and crop yield [J ] . Nature Plants , 2020 , 6 ( 5 ): 570 - 580 .

GIMPEL J A , NOUR-ELDIN H H , SCRANTON M A , et al . Refactoring the six-gene photosystem Ⅱ core in the chloroplast of the green algae Chlamydomonas reinhardtii [J ] . ACS Synthetic Biology , 2016 , 5 ( 7 ): 589 - 596 .

SHEN L L , TANG K L , WANG W D , et al . Architecture of the chloroplast PSⅠ-NDH super complex in Hordeum vulgare [J ] . Nature , 2022 , 601 ( 7894 ): 649 - 654 .

WU J H , CHEN S , WANG C , et al . Regulatory dynamics of the higher-plant PSⅠ-LHCⅠ super complex during state transitions [J ] . Molecular Plant , 2023 , 16 ( 12 ): 1937 - 1950 .

IWAI M , TAKIZAWA K , TOKUTSU R , et al . Isolation of the elusive super complex that drives cyclic electron flow in photosynthesis [J ] . Nature , 2010 , 464 ( 7292 ): 1210 - 1213 .

SHAN J Y , NIEDZWIEDZKI D M , TOMAR R S , et al . Architecture and functional regulation of a plant PSⅡ-LHCⅡ mega complex [J ] . Science Advances , 2024 , 10 ( 50 ): eadq9967 .

STIRBET A , LAZÁR D , GUO Y , et al . Photosynthesis: basics, history and modelling [J ] . Annals of Botany , 2020 , 126 ( 4 ): 511 - 537 .

PORTIS A R JR . Rubisco activase-Rubisco’s catalytic chaperone [J ] . Photosynthesis Research , 2003 , 75 ( 1 ): 11 - 27 .

SHARKEY T D , BADGER M R , VON CAEMMERER S , et al . Increased heat sensitivity of photosynthesis in tobacco plants with reduced Rubisco activase [J ] . Photosynthesis Research , 2001 , 67 ( 1-2 ): 147 - 156 .

WAHEEDA K , KITCHEL H , WANG Q , et al . Molecular mechanism of Rubisco activase: dynamic assembly and Rubisco remodeling [J ] . Frontiers in Molecular Biosciences , 2023 , 10 : 1125922 .

QU Y C , SAKODA K , FUKAYAMA H , et al . Overexpression of both Rubisco and Rubisco activase rescues rice photosynthesis and biomass under heat stress [J ] . Plant , Cell & Environment, 2021 , 44 ( 7 ): 2308 - 2320 .

BHAT J Y , THIEULIN-PARDO G , HARTL F U , et al . Rubisco activases: AAA+ chaperones adapted to enzyme repair [J ] . Frontiers in Molecular Biosciences , 2017 , 4 : 20 .

MORENO J , GARCÍA-MURRIA M J , MARÍN-NAVARRO J . Redox modulation of Rubisco conformation and activity through its cysteine residues [J ] . Journal of Experimental Botany , 2008 , 59 ( 7 ): 1605 - 1614 .

SUDHANI H P K , MORENO J . Control of the ribulose 1,5-bisphosphate carboxylase/oxygenase activity by the chloroplastic glutathione pool [J ] . Archives of Biochemistry and Biophysics , 2015 , 567 : 30 - 34 .

CARMO-SILVA E , SHARWOOD R E . Rubisco and its regulation: major advances to improve carbon assimilation and productivity [J ] . Journal of Experimental Botany , 2023 , 74 ( 2 ): 507 - 509 .

BAR-ON Y M , MILO R . The global mass and average rate of Rubisco [J ] . Proceedings of the National Academy of Sciences of the United States of America , 2019 , 116 ( 10 ): 4738 - 4743 .

JENSEN R G . Activation of Rubisco regulates photosynthesis at high temperature and CO 2 [J ] . Proceedings of the National Academy of Sciences of the United States of America , 2000 , 97 ( 24 ): 12937 - 12938 .

BRACHER A , WHITNEY S M , HARTL F U , et al . Biogenesis and metabolic maintenance of Rubisco [J ] . Annual Review of Plant Biology , 2017 , 68 : 29 - 60 .

SPREITZER R J , SALVUCCI M E . Rubisco: structure, regulatory interactions, and possibilities for a better enzyme [J ] . Annual Review of Plant Biology , 2002 , 53 : 449 - 475 .

ELLIS R J . The most abundant protein in the world [J ] . Trends in Biochemical Sciences , 1979 , 4 ( 11 ): 241 - 244 .

KU M S B , SCHMITT M R , EDWARDS G E . Quantitative determination of RuBP carboxylase-oxygenase protein in leaves of several C 3 and C 4 plants [J ] . Journal of Experimental Botany , 1979 , 30 ( 1 ): 89 - 98 .

PARIKH M R , GREENE D N , WOODS K K , et al . Directed evolution of RuBisCO hypermorphs through genetic selection in engineered E . coli [J ] . Protein Engineering , Design & Selection, 2006 , 19 ( 3 ): 113 - 119 .

MUELLER-CAJAR O , MORELL M , WHITNEY S M . Directed evolution of rubisco in Escherichia coli reveals a specificity-determining hydrogen bond in the form Ⅱ enzyme [J ] . Biochemistry , 2007 , 46 ( 49 ): 14067 - 14074 .

DURÃO P , AIGNER H , NAGY P , et al . Opposing effects of folding and assembly chaperones on evolvability of Rubisco [J ] . Nature Chemical Biology , 2015 , 11 ( 2 ): 148 - 155 .

CAI Z , LIU G X , ZHANG J L , et al . Development of an activity-directed selection system enabled significant improvement of the carboxylation efficiency of Rubisco [J ] . Protein & Cell , 2014 , 5 ( 7 ): 552 - 562 .

WILSON R H , ALONSO H , WHITNEY S M . Evolving Methanococcoides burtonii archaeal Rubisco for improved photosynthesis and plant growth [J ] . Scientific Reports , 2016 , 6 : 22284 .

PRYWES N , PHILLIPS N R , OLTROGGE L M , et al . A map of the Rubisco biochemical landscape [J ] . Nature , 2025 , 638 ( 8051 ): 823 - 828 .

LIN M T , STONE W D , CHAUDHARI V , et al . Small subunits can determine enzyme kinetics of tobacco Rubisco expressed in Escherichia coli [J ] . Nature Plants , 2020 , 6 ( 10 ): 1289 - 1299 .

AIGNER H , WILSON R H , BRACHER A , et al . Plant RuBisCo assembly in E . coli with five chloroplast chaperones including BSD2 [J ] . Science , 2017 , 358 ( 6368 ): 1272 - 1278 .

BUCK S , RHODES T , GIONFRIDDO M , et al . Escherichia coli expressing chloroplast chaperones as a proxy to test heterologous Rubisco production in leaves [J ] . Journal of Experimental Botany , 2023 , 74 ( 2 ): 664 - 676 .

IQBAL W A , LISITSA A , KAPRALOV M V . Predicting plant Rubisco kinetics from RbcL sequence data using machine learning [J ] . Journal of Experimental Botany , 2023 , 74 ( 2 ): 638 - 650 .

GIONFRIDDO M , RHODES T , WHITNEY S M . Perspectives on improving crop Rubisco by directed evolution [J ] . Seminars in Cell & Developmental Biology , 2024 , 155 : 37 - 47 .

ZHU X G , LONG S P , ORT D R . Improving photosynthetic efficiency for greater yield [J ] . Annual Review of Plant Biology , 2010 , 61 : 235 - 261 .

CHAO M N , HU G H , DONG J , et al . Sequence cha racteristics and expression analysis of the gene encoding sedoheptulose-1,7-bisphosphatase, an important Calvin cycle enzyme in upland cotton ( Gossypium hirsutum L.) [J ] . International Journal of Molecular Sciences , 2023 , 24 ( 7 ): 6648 .

WANG M L , BI H G , LIU P P , et al . Molecular cloning and expression analysis of the gene encoding sedoheptulose-1,7-bisphosphatase from Cucumis sativus [J ] . Scientia Horticulturae , 2011 , 129 ( 3 ): 414 - 420 .

LEFEBVRE S , LAWSON T , ZAKHLENIUK O V , et al . Increased sedoheptulose-1,7-bisphosphatase activity in transgenic tobacco plants stimulates photosynthesis and growth from an early stage in development [J ] . Plant Physiology , 2005 , 138 ( 2 ): 1174 - 1174 .

LIU X L , YU H D , GUAN Y , et al . Carbonylation and loss-of-function analyses of SBPase reveal its metabolic interface role in oxidative stress, carbon assimilation, and multiple aspects of growth and development in Arabidopsis [J ] . Molecular Plant , 2012 , 5 ( 5 ): 1082 - 1099 .

SCHURMANN P , JACQUOT J P . Plant thioredoxin systems revisited [J ] . Annual Review of Plant Physiology and Plant Molecular Biology , 2000 , 51 : 371 - 400 .

THIEULIN-PARDO G , REMY T , LIGNON S , et al . Phosphoribulokinase from Chlamydomonas reinhardtii : a Benson-Calvin cycle enzyme enslaved to its cysteine residues [J ] . Molecular BioSystems , 2015 , 11 ( 4 ): 1134 - 1145 .

MARRI L , ZAFFAGNINI M , COLLIN V , et al . Prompt and easy activation by specific thioredoxins of Calvin cycle enzymes of Arabidopsis thaliana associated in the GAPDH/CP12/PRK supramolecular complex [J ] . Molecular Plant , 2009 , 2 ( 2 ): 259 - 269 .

ZHU X G , DE STURLER E , LONG S P . Optimizing the distribution of resources between enzymes of carbon metabolism can dramatically increase photosynthetic rate: a numerical simulation using an evolutionary algorithm [J ] . Plant Physiology , 2007 , 145 ( 2 ): 513 - 526 .

SIMKIN A J , LOPEZ-CALCAGNO P E , DAVEY P A , et al . Simultaneous stimulation of sedoheptulose 1,7-bisphosphatase, fructose 1,6-bisphophate aldolase and the photorespiratory glycine decarboxylase-H protein increases CO 2 assimilation, vegetative biomass and seed yield in Arabidopsis [J ] . Plant Biotechnology Journal , 2017 , 15 ( 7 ): 805 - 816 .

SIMKIN A J , MCAUSLAND L , HEADLAND L R , et al . Multigene manipulation of photosynthetic carbon assimilation increases CO 2 fixation and biomass yield in tobacco [J ] . Journal of Experimental Botany , 2015 , 66 ( 13 ): 4075 - 4090 .

DING F , WANG M L , ZHANG S X , et al . Changes in SBPase activity influence photosynthetic capacity, growth, and tolerance to chilling stress in transgenic tomato plants [J ] . Scientific Reports , 2016 , 6 : 32741 .

DRIEVER S M , SIMKIN A J , ALOTAIBI S , et al . Increased SBPase activity improves photosynthesis and grain yield in wheat grown in greenhouse conditions [J ] . Philosophical Transactions of the Royal Society of London Series B , Biological Sciences, 2017 , 372 ( 1730 ): 20160384 .

ZHAO H L , TANG Q M , CHANG T G , et al . Why an increase in activity of an enzyme in the Calvin-Benson cycle does not always lead to an increased photosynthetic CO 2 uptake rate?—a theoretical analysis [J ] . In Silico Plants , 2021 , 3 ( 1 ): diaa009 .

SAGE R F , CHRISTIN P A , EDWARDS E J . The C 4 plant lineages of planet Earth [J ] . Journal of Experimental Botany , 2011 , 62 ( 9 ): 3155 - 3169 .

SAGE R F , SAGE T L , KOCACINAR F . Photorespiration and the evolution of C 4 photosynthesis [J ] . Annual Review of Plant Biology , 2012 , 63 : 19 - 47 .

YAMORI W , HIKOSAKA K , WAY D A . Temperature response of photosynthesis in C 3 , C 4 , and CAM plants: temperature acclimation and temperature adaptation [J ] . Photosynthesis Research , 2014 , 119 ( 1 ): 101 - 117 .

LONG B M , HEE W Y , SHARWOOD R E , et al . Carboxysome encapsulation of the CO 2 -fixing enzyme Rubisco in tobacco chloroplasts [J ] . Nature Communications , 2018 , 9 : 3570 .

RAE B D , LONG B M , FÖRSTER B , et al . Progress and challenges of engineering a biophysical CO 2 -concentrating mechanism into higher plants [J ] . Journal of Experimental Botany , 2017 , 68 ( 14 ): 3717 - 3737 .

HE S , CRANS V L , JONIKAS M C . The pyrenoid: the eukaryotic CO 2 -concentrating organelle [J ] . The Plant Cell , 2023 , 35 ( 9 ): 3236 - 3259 .

LONG B M , RAE B D , ROLLAND V , et al . Cyanobacterial CO 2 -concentrating mechanism components : function and prospects for plant metabolic engineering [J ] . Current Opinion in Plant Biology , 2016 , 31 : 1 - 8 .

HIBBERD J M , QUICK W P . Characteristics of C 4 photosynthesis in stems and petioles of C 3 flowering plants [J ] . Nature , 2002 , 415 ( 6870 ): 451 - 454 .

SCHULER M L , MANTEGAZZA O , WEBER A P M . Engineering C 4 photosynthesis into C 3 chassis in the synthetic biology age [J ] . The Plant Journal , 2016 , 87 ( 1 ): 51 - 65 .

CHEN T Y , HOJKA M , DAVEY P , et al . Engineering α-carboxysomes into plant chloroplasts to support autotrophic photosynthesis [J ] . Nature Communications , 2023 , 14 : 2118 .

QIN K Z , YE X Y , LUO S S , et al . Engineering carbon assimilation in plants [J ] . Journal of Integrative Plant Biology , 2025 , 67 ( 4 ): 926 - 948 .

FEI C Y , WILSON A T , MANGAN N M , et al . Modelling the pyrenoid-based CO 2 -concentrating mechanism provides insights into its operating principles and a roadmap for its engineering into crops [J ] . Nature Plants , 2022 , 8 ( 5 ): 583 - 595 .

ATKINSON N , MAO Y W , CHAN K X , et al . Condensation of Rubisco into a proto-pyrenoid in higher plant chloroplasts [J ] . Nature Communications , 2020 , 11 : 6303 .

凌丽俐 , 林宏辉 , 焦德茂 . 转PEPC基因水稻种质的稳定光合生理特性 [J ] . 作物学报 , 2006 , 32 ( 4 ): 527 - 531 .

LING L L , LIN H H , JIAO D M . The stable photosynthetic characteristics of a PEPC transgenic rice germplasm [J ] . Acta Agronomica Sinica , 2006 , 32 ( 4 ): 527 - 531 .

ERMAKOVA M , ARRIVAULT S , GIULIANI R , et al . Installation of C 4 photosynthetic pathway enzymes in rice using a single construct [J ] . Plant Biotechnology Journal , 2021 , 19 ( 3 ): 575 - 588 .

SWIFT J , LUGINBUEHL L H , HUA L , et al . Exaptation of ancestral cell-identity networks enables C 4 photosynthesis [J ] . Nature , 2024 , 636 ( 8041 ): 143 - 150 .

SEDELNIKOVA O V , HUGHES T E , LANGDALE J A . Understanding the genetic basis of C 4 Kranz anatomy with a view to engineering C 3 crops [J ] . Annual Review of Genetics , 2018 , 52 : 249 - 270 .

BAUWE H . Photorespiration-Rubisco’s repair crew [J ] . Journal of Plant Physiology , 2023 , 280 : 153899 .

SOUTH P F , CAVANAGH A P , LIU H W , et al . Synthetic glycolate metabolism pathways stimulate crop growth and productivity in the field [J ] . Science , 2019 , 363 ( 6422 ): eaat9077 .

ROELL M S , SCHADA VON BORZYKOWSKI L , WESTHOFF P , et al . A synthetic C 4 shuttle via the β-hydroxyaspartate cycle in C 3 plants [J ] . Proceedings of the National Academy of Sciences of the United States of America , 2021 , 118 ( 21 ): e2022307118 .

SHEN B R , WANG L M , LIN X L , et al . Engineering a new chloroplastic photorespiratory bypass to increase photosynthetic efficiency and productivity in rice [J ] . Molecular Plant , 2019 , 12 ( 2 ): 199 - 214 .

WANG L M , SHEN B R , LI B D , et al . A synthetic photorespiratory shortcut enhances photosynthesis to boost biomass and grain yield in rice [J ] . Molecular Plant , 2020 , 13 ( 12 ): 1802 - 1815 .

XU H W , WANG H H , ZHANG Y W , et al . A synthetic light-inducible photorespiratory bypass enhances photosynthesis to improve rice growth and grain yield [J ] . Plant Communications , 2023 , 4 ( 6 ): 100641 .

CHEN G X , LI Y N , JIN K N , et al . Synthetic photorespiratory bypass improves rice productivity by enhancing photosynthesis and nitrogen uptake [J ] . The Plant Cell , 2025 , 37 ( 1 ): koaf015 .

ERB T J . Photosynthesis 2.0: realizing new-to-nature CO 2 -fixation to overcome the limits of natural metabolism [J ] . Cold Spring Harbor Perspectives in Biology , 2024 , 16 ( 2 ): a041669 .

SCHEFFEN M , MARCHAL D G , BENEYTON T , et al . A new-to-nature carboxylation module to improve natural and synthetic CO 2 fixation [J ] . Nature Catalysis , 2021 , 4 ( 2 ): 105 - 115 .

CHI W , SUN X W , ZHANG L X . The roles of chloroplast proteases in the biogenesis and maintenance of photosystem Ⅱ [J ] . Biochimica et Biophysica Acta (BBA)-Bioenergetics , 2012 , 1817 ( 1 ): 239 - 246 .

MU X H , CHEN Q W , CHEN F J , et al . Within-leaf nitrogen allocation in adaptation to low nitrogen supply in maize during grain-filling stage [J ] . Frontiers in Plant Science , 2016 , 7 : 699 .

SHARMA A , KUMAR V , SINGH R , et al . Effect of seed pre-soaking with 24-epibrassinolide on growth and photosynthetic parameters of Brassica juncea L. in imidacloprid soil [J ] . Ecotoxicology and Environmental Safety , 2016 , 133 : 195 - 201 .

SHARMA A , SHAHZAD B , KUMAR V , et al . Phytohormones regulate accumulation of osmolytes under abiotic stress [J ] . Biomolecules , 2019 , 9 ( 7 ): 285 .

KAUR R , YADAV P , SHARMA A , et al . Castasterone and citric acid treatment restores photosynthetic attributes in Brassica juncea L. under Cd(Ⅱ) toxicity [J ] . Ecotoxicology and Environmental Safety , 2017 , 145 : 466 - 475 .

DEMMIG-ADAMS B , STEWART J J , BAKER C R , et al . Optimization of photosynthetic productivity in contrasting environments by regulons controlling plant form and function [J ] . International Journal of Molecular Sciences , 2018 , 19 ( 3 ): 872 .

KOHLI S K , HANDA N , SHARMA A , et al . Interaction of 24-epibrassinolide and salicylic acid regulates pigment contents, antioxidative defense responses, and gene expression in Brassica juncea L. seedlings under Pb stress [J ] . Environmental Science and Pollution Research , 2018 , 25 ( 15 ): 15159 - 15173 .

PAUNOV M , KOLEVA L , VASSILEV A , et al . Effects of different metals on photosynthesis: cadmium and zinc affect chlorophyll fluorescence in durum wheat [J ] . International Journal of Molecular Sciences , 2018 , 19 ( 3 ): 787 .

SOARES C , BRANCO-NEVES S , DE SOUSA A , et al . SiO 2 nanomaterial as a tool to improve Hordeum vulgare L. tolerance to nano-NiO stress [J ] . Science of the Total Environment , 2018 , 622-623 : 517 - 525 .

YADAV P , KAUR R , KANWAR M K , et al . Castasterone confers copper stress tolerance by regulating antioxidant enzyme responses, antioxidants, and amino acid balance in B. juncea seedlings [J ] . Ecotoxicology and Environmental Safety , 2018 , 147 : 725 - 734 .

XIA X J , HUANG Y Y , WANG L , et al . Pesticides-induced depression of photosynthesis was alleviated by 24-epibrassinolide pretreatment in Cucumis sativus L [J ] . Pesticide Biochemistry and Physiology , 2006 , 86 ( 1 ): 42 - 48 .

KALAJI H M , JAJOO A , OUKARROUM A , et al . Chlorophyll a fluorescence as a tool to monitor physiological status of plants under abiotic stress conditions [J ] . Acta Physiologiae Plantarum , 2016 , 38 ( 4 ): 102 .

SHARMA A , THAKUR S , KUMAR V , et al . Pre-sowing seed treatment with 24-epibrassinolide ameliorates pesticide stress in Brassica juncea L. through the modulation of stress markers [J ] . Frontiers in Plant Science , 2016 , 7 : 1569 .

GURURANI M A , VENKATESH J , TRAN L S P . Regulation of photosynthesis during abiotic stress-induced photoinhibition [J ] . Molecular Plant , 2015 , 8 ( 9 ): 1304 - 1320 .

CHAVES M M , FLEXAS J , PINHEIRO C . Photosynthesis under drought and salt stress: regulation mechanisms from whole plant to cell [J ] . Annals of Botany , 2009 , 103 ( 4 ): 551 - 560 .

ZHANG X F , ZHANG X H , GAO B , et al . Effect of cadmium on growth, photosynthesis, mineral nutrition and metal accumulation of an energy crop, king grass ( Pennisetum americanum × P. purpureum ) [J ] . Biomass & Bioenergy , 2014 , 67 : 179 - 187 .

KOHLI S K , HANDA N , SHARMA A , et al . Synergistic effect of 24-epibrassinolide and salicylic acid on photosynthetic efficiency and gene expression in Brassica juncea L. under Pb stress [J ] . Turkish Journal of Biology , 2017 , 41 ( 6 ): 943 - 953 .

MUHAMMAD I , SHALMANI A , ALI M , et al . Mechanisms regulating the dynamics of photosynthesis under abiotic stresses [J ] . Frontiers in Plant Science , 2021 , 11 : 615942 .

RUBAN A V . Nonphotochemical chlorophyll fluorescence quenching: mechanism and effectiveness in protecting plants from photodamage [J ] . Plant Physiology , 2016 , 170 ( 4 ): 1903 - 1916 .

CAZZANIGA S , DALL′OSTO L , KONG S G , et al . Interaction between avoidance of photon absorption, excess energy dissipation and Zeaxanthin synthesis against photooxidative stress in Arabidopsis [J ] . The Plant Journal , 2013 , 76 ( 4 ): 568 - 579 .

ZHU X G , ORT D R , WHITMARSH J , et al . The slow reversibility of photosystem Ⅱ thermal energy dissipation on transfer from high to low light may cause large losses in carbon gain by crop canopies: a theoretical analysis [J ] . Journal of Experimental Botany , 2004 , 55 ( 400 ): 1167 - 1175 .

HUANG W , HU H , ZHANG S B . Photorespiration plays an important role in the regulation of photosynthetic electron flow under fluctuating light in tobacco plants grown under full sunlight [J ] . Frontiers in Plant Science , 2015 , 6 : 621 .

LAUREAU C , DE PAEPE R , LATOUCHE G , et al . Plastid terminal oxidase ( PTOX ) has the potential to act as a safety valve for excess excitation energy in the alpine plant species Ranunculus glacialis L. [J ] . Plant , Cell & Environment, 2013 , 36 ( 7 ): 1296 - 1310 .

DE SOUZA A P , BURGESS S J , DORAN L , et al . Soybean photosynthesis and crop yield are improved by accelerating recovery from photoprotection [J ] . Science , 2022 , 377 ( 6608 ): 851 - 854 .

KROMDIJK J , GŁOWACKA K , LEONELLI L , et al . Improving photosynthesis and crop productivity by accelerating recovery from photoprotection [J ] . Science , 2016 , 354 ( 6314 ): 857 - 861 .

CUI L L , LU Y S , LI Y , et al . Overexpression of glycolate oxidase confers improved photosynthesis under high light and high temperature in rice [J ] . Frontiers in Plant Science , 2016 , 7 : 1165 .

SCAFARO A P , ATWELL B J , MUYLAERT S , et al . A thermotolerant variant of Rubisco activase from a wild relative improves growth and seed yield in rice under heat stress [J ] . Frontiers in Plant Science , 2018 , 9 : 1663 .

LEISTER D . How can the light reactions of photosynthesis be improved in plants? [J ] . Frontiers in Plant Science , 2012 , 3 : 199 .

BATISTA-SILVA W , DA FONSECA-PEREIRA P , MARTINS A O , et al . Engineering improved photosynthesis in the era of synthetic biology [J ] . Plant Communications , 2020 , 1 ( 2 ): 100032 .

KUBIS A , BAR-EVEN A . Synthetic biology approaches for improving photosynthesis [J ] . Journal of Experimental Botany , 2019 , 70 ( 5 ): 1425 - 1433 .

CROCE R , CARMO-SILVA E , CHO Y B , et al . Perspectives on improving photosynthesis to increase crop yield [J ] . The Plant Cell , 2024 , 36 ( 10 ): 3944 - 3973 .

LI B D , HUANG A Y , WANG L M , et al . Increased sugar content impairs pollen fertility and reduces seed-setting in high-photosynthetic-efficiency rice [J ] . The Crop Journal , 2024 , 12 ( 6 ): 1547 - 1558 .

ERMAKOVA M , DANILA F R , FURBANK R T , et al . On the road to C 4 rice: advances and perspectives [J ] . The Plant Journal , 2020 , 101 ( 4 ): 940 - 950 .

浏览量

下载量

CSCD

文章被引用时，请邮件提醒。

提交

工具集

关联资源

光合作用碳同化的合成生物学研究进展

合成生物学赋能细胞膜纳米颗粒的精准诊疗

合成生物学策略下的人工血液研究进展