过氧化物酶的重组表达和应用研究进展

李庚; 申晓林; 孙新晓; 王佳; 袁其朋

doi:10.12211/2096-8280.2024-027

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过氧化物酶的重组表达和应用研究进展

特约评述 | 更新时间：2025-04-11

- 过氧化物酶的重组表达和应用研究进展
- Research progress in recombinant expression and application of peroxidases
- 合成生物学 2024年5卷第6期页码：1498-1517
- 作者机构：
  
  北京化工大学生命科学与技术学院，化工资源有效利用国家重点实验室，北京 100029
- 作者简介：
  
  [ "李庚（1998—），男，硕士研究生。研究方向为代谢工程及合成生物学。E-mail：1602975489@qq.com" ]
  [ "王佳（1989—），女，博士，教授。研究方向为代谢工程及合成生物学。E-mail：wangjia@mail.buct.edu.cn" ]
  [ "袁其朋（1969—），男，博士，教授。研究方向为生物化工，代谢工程及微生物合成生物学。E-mail：yuanqp@mail.buct.edu.cn" ]
- 基金信息：
  
  国家重点研发计划(2022YFC2106100);国家自然科学基金(22378016)
- DOI：10.12211/2096-8280.2024-027
  中图分类号： Q812
- 收稿：2024-03-21，
  
  修回：2024-06-28，
  
  纸质出版：2024-12-31
- 稿件说明：
移动端阅览
李庚，申晓林，孙新晓，王佳，袁其朋. 过氧化物酶的重组表达和应用研究进展［J］. 合成生物学， 2024， 5（6）： 1498-1517

LI Geng， SHEN Xiaolin， SUN Xinxiao， WANG Jia， YUAN Qipeng. Research progress in recombinant expression and application of peroxidases［J］. Synthetic Biology Journal， 2024， 5（6）： 1498-1517
李庚，申晓林，孙新晓，王佳，袁其朋. 过氧化物酶的重组表达和应用研究进展［J］. 合成生物学， 2024， 5（6）： 1498-1517 DOI： 10.12211/2096-8280.2024-027.

LI Geng， SHEN Xiaolin， SUN Xinxiao， WANG Jia， YUAN Qipeng. Research progress in recombinant expression and application of peroxidases［J］. Synthetic Biology Journal， 2024， 5（6）： 1498-1517 DOI： 10.12211/2096-8280.2024-027.

摘要

过氧化物酶作为一类自然界中广泛分布的酶，参与了生物体的先天免疫防疫、植物微生物抗氧化应激、真菌木质素降解、植物细胞壁代谢及伤口愈合等重要生命过程。随着测序、基因编辑、重组蛋白表达和高通量筛选技术的飞速发展，越来越多的过氧化物酶被发现、表征和重组表达。这些种类丰富、数量庞大及催化性能卓越的过氧化物酶，在众多领域的应用研究中受到广泛关注。近年来过氧化物酶在重组表达上取得了显著进展，进一步促进了其在应用研究领域的开发。本文从系统进化分类及功能角度对过氧化物酶进行了简要概述，对近年来过氧化物酶在大肠杆菌、酵母和丝状真菌中重组表达研究进展及其在环境修复、化合物检测的应用研究成果进行系统综述，重点介绍了过氧化物酶应用于生物合成高附加值化合物方面的最新研究进展，并对其目前在该领域应用研究中存在的底物和产物非专一性问题及辅因子H

细胞毒性问题进行讨论。过氧化物酶在医学检测、环境保护和生物合成等领域中的应用潜力巨大。然而，当前的技术和应用仍面临一些挑战，比如过氧化物酶在复杂环境中的稳定性和活性差、酶制剂生产成本高及专一性差问题。未来，通过结合蛋白质工程、合成生物学和固定化技术等多学科的最新进展，可以有效解决这些挑战，推动过氧化物酶在各个领域的广泛应用。

Abstract

As a class of enzymes widely distributed in nature

peroxidases are involved in important life processes such as innate immunity and epidemic prevention of organisms

anti-oxidative stress of plant microorganisms

fungal lignin degradation

plant ce

ll wall metabolism and wound healing. With the rapid development of DNA sequencing

gene editing

recombinant protein expression and high-throughput screening technologies

more and more peroxidases have been discovered

characterized and recombinantly expressed. These peroxidases

characterized by their species diversity

abundant quantity

and excellent catalytic performance

have attracted extensive attention in many fields of application research. In recent years

remarkable progress has been made in the recombinant expression of peroxidases

further promoting their development in the field of applied research. Additionally

as we deepen our understanding of the catalytic properties of peroxidases

new opportunities have emerged for their application in the field of biosynthesis. Their high catalytic activity allows for rapid oxidation reactions under mild conditions and enables the construction of multi-enzyme cascade systems in conjunction with other enzymes

thereby facilitating the efficient synthesis of complex compounds. This paper provides a brief overview of peroxidases from the perspective of systematic evolutionary classification and function. It systematically reviews recent progress in the recombinant expression of peroxidases in

Escherichia coli

yeast

and fungi

as well as their application achievements in environmental remediation and compound detection. The focus is on the latest research advances in the application of peroxidases for the biosynthesis of high-value-added compounds. The paper also discusses the current issues in this field

such as substrate and product non-specificity and the cytotoxicity of the cofactor H

. Peroxidases have enormous potential for applications in medical diagnostics

environmental protection

and biosynthesis. However

current technologies and applications still face several challenges

such as the stability and activity of peroxidases in complex environments

high production costs of enzyme preparations

and poor spec

ificity. In the future

by integrating the latest advances in protein engineering

synthetic biology

and immobilization technology

these challenges can be effectively solved

promoting the widespread application of peroxidases across various fields.

关键词

Keywords

references

SAVELLI B , LI Q , WEBBER M , et al . RedoxiBase: a database for ROS homeostasis regulated proteins [J ] . Redox Biology , 2019 , 26 : 101247 .

ZAMOCKY M , JAKOPITSCH C , FURTMÜLLER P G , et al . The peroxidase-cyclooxygenase superfamily: reconstructed evolution of critical enzymes of the innate immune system [J ] . Proteins: Structure, Function, and Bioinformatics , 2008 , 72 ( 2 ): 589 - 605 .

KANGASJÄRVI S , LEPISTÖ A , HÄNNIKÄINEN K , et al . Diverse roles for chloroplast stromal and thylakoid-bound ascorbate peroxidases in plant stress responses [J ] . The Biochemical Journal , 2008 , 412 ( 2 ): 275 - 285 .

PREETHI P S , PATHY M R . Recombinant peroxidase: production and its prospective applications-a review [J ] . Research Journal of Pharmacy and Technology , 2018 , 11 ( 7 ): 3186 - 3196 .

MARTINS D , KATHIRESAN M , ENGLISH A M . Cytochrome c peroxidase is a mitochondrial heme-based H 2 O 2 sensor that modulates antioxidant defense [J ] . Free Radical Biology and Medicine , 2013 , 65 : 541 - 551 .

HIGUCHI T . Microbial degradation of lignin: role of lignin peroxidase, manganese peroxidase, and laccase [J ] . Proceedings of the Japan Academy, Series B , 2004 , 80 ( 5 ): 204 - 214 .

SIGOILLOT J C , BERRIN J G , BEY M , et al . Fungal strategies for lignin degradation [M/OL ] // Lignins: biosynthesis, biodegradation and bioengineering . Amsterdam : Elsevier , 2012 : 263 - 308 . ( 2012-06-08)[2024-03-01] . https://doi.org/10.1016/B978-0-12-416023-1.00008-2 https://doi.org/10.1016/B978-0-12-416023-1.00008-2 .

COSIO C , DUNAND C . Specific functions of individual class Ⅲ peroxidase genes [J ] . Journal of Experimental Botany , 2009 , 60 ( 2 ): 391 - 408 .

PASSARDI F , PENEL C , DUNAND C . Performing the paradoxical: how plant peroxidases modify the cell wall [J ] . Trends in Plant Science , 2004 , 9 ( 11 ): 534 - 540 .

BARCELÓ A R , POMAR F . Oxidation of cinnamyl alcohols and aldehydes by a basic peroxidase from lignifying Zinnia elegans hypocotyls [J ] . Phytochemistry , 2001 , 57 ( 7 ): 1105 - 1113 .

BERNARDS M A , FLEMING W D , LLEWELLYN D B , et al . Biochemical characterization of the suberization-associated anionic peroxidase of potato [J ] . Plant Physiology , 1999 , 121 ( 1 ): 135 - 146 .

GAZARYAN I G , LAGRIMINI L M , ASHBY G A , et al . Mechanism of indole-3-acetic acid oxidation by plant peroxidases: anaerobic stopped-flow spectrophotometric studies on horseradish and tobacco peroxidases [J ] . The Biochemical Journal , 1996 , 313 ( 3 ): 841 - 847 .

ALLISON S D , SCHULTZ J C . Differential activity of peroxidase isozymes in response to wounding, gypsy moth, and plant hormones in northern red oak ( Quercus rubra L.) [J ] . Journal of Chemical Ecology , 2004 , 30 ( 7 ): 1363 - 1379 .

LISZKAY A , KENK B , SCHOPFER P . Evidence for the involvement of cell wall peroxidase in the generation of hydroxyl radicals mediating extension growth [J ] . Planta , 2003 , 217 ( 4 ): 658 - 667 .

MCINNIS S M , DESIKAN R , HANCOCK J T , et al . Production of reactive oxygen species and reactive nitrogen species by angiosperm stigmas and pollen: potential signalling crosstalk? [J ] . The New Phytologist , 2006 , 172 ( 2 ): 221 - 228 .

HUANG R H , XIA R X , HU L M , et al . Antioxidant activity and oxygen-scavenging system in orange pulp during fruit ripening and maturation [J ] . Scientia Horticulturae , 2007 , 113 ( 2 ): 166 - 172 .

PANDEY V P , AWASTHI M , SINGH S , et al . A comprehensive review on function and application of plant peroxidases [J ] . Biochemistry & Analytical Biochemistry , 2017 , 6 ( 1 ): 308 .

LIN Z L , THORSEN T , ARNOLD F H . Functional expression of horseradish peroxidase in E . coli by directed evolution [J ] . Biotechnology Progress , 1999 , 15 ( 3 ): 467 - 471 .

ASAD S , DABIRMANESH B , GHAEMI N , et al . Studies on the refolding process of recombinant horseradish peroxidase [J ] . Molecular Biotechnology , 2013 , 54 ( 2 ): 484 - 492 .

GUNDINGER T , SPADIUT O . A comparative approach to recombinantly produce the plant enzyme horseradish peroxidase in Escherichia coli [J ] . Journal of Biotechnology , 2017 , 248 : 15 - 24 .

CHAUHAN S , KANG T J . Soluble expression of horseradish peroxidase in Escherichia coli and its facile activation [J ] . Journal of Bioscience and Bioengineering , 2018 , 126 ( 4 ): 431 - 435 .

WANG N , REN K , JIA R , et al . Expression of a fungal manganese peroxidase in Escherichia coli : a comparison between the soluble and refolded enzymes [J ] . BMC Biotechnology , 2016 , 16 ( 1 ): 87 .

LIN M I , NAGATA T , KATAHIRA M . High yield production of fungal manganese peroxidases by E . coli through soluble expression, and examination of the activities [J ] . Protein Expression and Purification , 2018 , 145 : 45 - 52 .

ALFI A , ZHU B , DAMNJANOVIĆ J , et al . Production of active manganese peroxidase in Escherichia coli by co-expression of chaperones and in vitro maturation by ATP-dependent chaperone release [J ] . Journal of Bioscience and Bioengineering , 2019 , 128 ( 3 ): 290 - 295 .

GU L N , LAJOIE C , KELLY C . Expression of a Phanerochaete chrysosporium manganese peroxidase gene in the yeast Pichia pastoris [J ] . Biotechnology Progress , 2003 , 19 ( 5 ): 1403 - 1409 .

MORAWSKI B , LIN Z L , CIRINO P , et al . Functional expression of horseradish peroxidase in Saccharomyces cerevisiae and Pichia pastoris [J ] . Protein Engineering , 2000 , 13 ( 5 ): 377 - 384 .

MORAWSKI B , QUAN S , ARNOLD F H . Functional expression and stabilization of horseradish peroxidase by directed evolution in Saccharomyces cerevisiae [J ] . Biotechnology and Bioengineering , 2001 , 76 ( 2 ): 99 - 107 .

GMEINER C , SPADIUT O . Effects of different media supplements on the production of an active recombinant plant peroxidase in a Pichia pastoris Δ och1 strain [J ] . Bioengineered , 2015 , 6 ( 3 ): 175 - 178 .

KRAINER F W , DARNHOFER B , BIRNER-GRUENBERGER R , et al . Recombinant production of a peroxidase-protein G fusion protein in Pichia pastoris [J ] . Journal of Biotechnology , 2016 , 219 : 24 - 27 .

KRAINER F W , GERSTMANN M A , DARNHOFER B , et al . Biotechnological advances towards an enhanced peroxidase production in Pichia pastoris [J ] . Journal of Biotechnology , 2016 , 233 : 181 - 189 .

CONESA A , JEENES D , ARCHER D B , et al . Calnexin overexpression increases manganese peroxidase production in Aspergillus niger [J ] . Applied and Environmental Microbiology , 2002 , 68 ( 2 ): 846 - 851 .

STEWART P , WHITWAM R E , KERSTEN P J , et al . Efficient expression of a Phanerochaete chrysosporium manganese peroxidase gene in Aspergillus oryzae [J ] . Applied and Environmental Microbiology , 1996 , 62 ( 3 ): 860 - 864 .

KAUR J , KUMAR A , KAUR J . Strategies for optimization of heterologous protein expression in E . coli : roadblocks and reinforcements [J ] . International Journal of Biological Macromolecules , 2018 , 106 : 803 - 822 .

WANG Y T , HAN H , CUI B Q , et al . A glutathione peroxidase from Antarctic psychrotrophic bacterium Pseudoalteromonas sp . ANT506: cloning and heterologous expression of the gene and characterization of recombinant enzyme [J ] . Bioengineered , 2017 , 8 ( 6 ): 742 - 749 .

LIAO J , WANG K Y , YAO W R , et al . Cloning, expression and antioxidant activity of a thioredoxin peroxidase from Branchiostoma belcheri tsingtaunese [J ] . PLoS One , 2017 , 12 ( 4 ): e0175162 .

KHAN S I , ZADA N S , SAHINKAYA M , et al . Cloning, expression and biochemical characterization of lignin-degrading DyP-type peroxidase from Bacillus sp. Strain BL5 [J ] . Enzyme and Microbial Technology , 2021 , 151 : 109917 .

CHEN W T , ZHENG L L , JIA R , et al . Cloning and expression of a new manganese peroxidase from Irpex lacteus F17 and its application in decolorization of reactive black 5 [J ] . Process Biochemistry , 2015 , 50 ( 11 ): 1748 - 1759 .

FATTAHIAN Y , RIAHI-MADVAR A , MIRZAEE R , et al . Heterologous expression, purification and characterization of a peroxidase isolated from Lepidium draba [J ] . The Protein Journal , 2017 , 36 ( 6 ): 461 - 471 .

GRIGORENKO V G , ANDREEVA I P , RUBTSOVA M Y , et al . Recombinant horseradish peroxidase: production and analytical applications [J ] . Biochemistry (Moscow) , 2015 , 80 ( 4 ): 408 - 416 .

YIN J C , LI G X , REN X F , et al . Select what you need: a comparative evaluation of the advantages and limitations of frequently used expression systems for foreign genes [J ] . Journal of Biotechnology , 2007 , 127 ( 3 ): 335 - 347 .

PECH-CANUL A C , CARRILLO-CAMPOS J , BALLINAS-CASARRUBIAS M L , et al . Functional expression and one-step protein purification of manganese peroxidase 1 (rMnP1) from Phanerochaetechrysosporium using the E . coli -expression system [J ] . International Journal of Molecular Sciences , 2020 , 21 ( 2 ): 416 .

XU H , GUO M Y , GAO Y H , et al . Expression and characteristics of manganese peroxida se from Ganoderma lucidum in Pichia pastoris and its application in the degradation of four dyes and phenol [J ] . BMC Biotechnology , 2017 , 17 ( 1 ): 19 .

WANG W , WEN X H . Expression of lignin peroxidase H 2 from Phanerochaete chrysosporium by multi-copy recombinant Pichia strain [J ] . Journal of Environmental Sciences , 2009 , 21 ( 2 ): 218 - 222 .

KIM S J , LEE J A , WON K , et al . Functional expression of Coprinus cinereus peroxidase in Pichia pastoris [J ] . Process Biochemistry , 2009 , 44 ( 7 ): 731 - 735 .

VLAMIS-GARDIKAS A , SMITH A T , CLEMENTS J M , et al . Expression of active horseradish peroxidase in Saccharomyces cerevisiae [J ] . Biochemical Society Transactions , 1992 , 20 ( 2 ): 111S .

KRAINER F W , GMEINER C , NEUTSCH L , et al . Knockout of an endogenous mannosyltransferase increases the homogeneity of glycoproteins produced in Pichia pastoris [J ] . Scientific Reports , 2013 , 3 : 3279 .

SU X Y , SCHMITZ G , ZHANG M L , et al . Heterologous gene expression in filamentous fungi [J ] . Advances in Applied Microbiology , 2012 , 81 : 1 - 61 .

CONESA A , VAN DEN HONDEL C A , PUNT P J . Studies on the production of fungal peroxidases in Aspergillus niger [J ] . Applied and Environmental Microbiology , 2000 , 66 ( 7 ): 3016 - 3023 .

CONESA A , VAN DE VELDE F , VAN RANTWIJK F , et al . Expression of the Caldariomyces fumago chloroperoxidase in Aspergillus niger and characterization of the recombinant enzyme [J ] . The Journal of Biological Chemistry , 2001 , 276 ( 21 ): 17635 - 17640 .

SHAKERI M , SUGANO Y , SHODA M . Stable repeated-batch production of recombinant dye-decolorizing peroxidase (rDyP) from Aspergillus oryzae [J ] . Journal of Bioscience and Bioengineering , 2008 , 105 ( 6 ): 683 - 686 .

MAYFIELD M B , KISHI K , ALIC M , et al . Homologous expression of recombinant manganese peroxidase in Phanerochaete chrysosporium [J ] . Applied and Environmental Microbiology , 1994 , 60 ( 12 ): 4303 - 4309 .

IRIE T , HONDA Y , WATANABE T , et al . Homologous expression of recombinant manganese peroxidase genes in ligninolytic fungus Pleurotus ostreatus [J ] . Applied Microbiology and Biotechnology , 2001 , 55 ( 5 ): 566 - 570 .

KAMEI I , TOMITAKA N , MOTODA T , et al . Selective homologous expression of recombinant manganese peroxi dase isozyme of salt-tolerant white-rot fungus Phlebia sp. MG-60, and its salt-tolerance and thermostability [J ] . Journal of Microbiology and Biotechnology , 2022 , 32 ( 2 ): 248 - 255 .

SALEHI S , ABDOLLAHI K , PANAHI R , et al . Applications of biocatalysts for sustainable oxidation of phenolic pollutants: a review [J ] . Sustainability , 2021 , 13 ( 15 ): 8620 .

CHATHA S A S , ASGHER M , IQBAL H M N . Enzyme-based solutions for textile processing and dye contaminant biodegradation-a review [J ] . Environmental Science and Pollution Research , 2017 , 24 ( 16 ): 14005 - 14018 .

COUTO C F , LANGE L C , AMARAL M C S . Occurrence, fate and removal of pharmaceutically active compounds (PhACs) in water and wastewater treatment plants — a review [J ] . Journal of Water Process Engineering , 2019 , 32 : 100927 .

SELLAMI K , COUVERT A , NASRALLAH N , et al . Peroxidase enzymes as green catalysts for bioremediation and biotechnological applications: a review [J ] . The Science of the Total Environment , 2022 , 806 ( Pt 2 ): 150500 .

BILAL M , RASHEED T , IQBAL H M N , et al . Peroxidases-assisted removal of environmentally-related hazardous pollutants with reference to the reaction mechanisms of industrial dyes [J ] . Science of the Total Environment , 2018 , 644 : 1 - 13 .

AZIZI A , ABOUSEOUD M , AHMEDI A . Phenol removal by soluble and alginate entrapped turnip peroxidase [J ] . Journal of Biochemical Technology , 2014 , 5 : 795 - 800 .

AZIZI A , ABOUSEOUD M , AMRANE A . Phenol removal by a sequential combined Fenton-enzymatic process [J ] . Nature Environment & Pollution Technology , 2017 , 16 ( 1 ): 321 - 330 .

ALEMZADEH I , NEJATI S . Phenols removal by immobilized horseradish peroxidase [J ] . Journal of Hazardous Materials , 2009 , 166 ( 2/3 ): 1082 - 1086 .

VASILEVA N , GODJEVARGOVA T , IVANOVA D , et al . Application of immobilized horseradish peroxidase onto modified acrylonitrile copolymer membrane in removing of phenol from water [J ] . International Journal of Biological Macromolecules , 2009 , 44 ( 2 ): 190 - 194 .

SELLAMI K , COUVERT A , NASRALLAH N , et al . Bio-based and cost-effective method for phenolic compounds removal using cross-linked enzyme aggregates [J ] . Journal of Hazardous Materials , 2021 , 403 : 124021 .

BAYRAMOĞLU G , ARıCA M Y . Enzymatic removal of phenol and p -chlorophenol in enzyme reactor: horseradish peroxidase immobilized on magnetic beads [J ] . Journal of Hazardous Materials , 2008 , 156 ( 1-3 ): 148 - 155 .

BESHARATI VINEH M , SABOURY A A , POOSTCHI A A , et al . Physical adsorption of horseradish peroxidase on reduced graphene oxide nanosheets functionalized by amine: a good system for biodegradation of high phenol concentration in wastewater [J ] . International Journal of Environmental Research , 2018 , 12 ( 1 ): 45 - 57 .

ASHRAF H , HUSAIN Q . Application of immobilized peroxidase for the removal of p -bromophenol from polluted water in batch and continuous processes [J ] . Journal of Water Reuse and Desalination , 2011 , 1 ( 1 ): 52 - 60 .

PETRONIJEVIĆ M , PANIĆ S , SAVIĆ S , et al . Characterization and application of biochar-immobilized crude horseradish peroxidase for removal of phenol from water [J ] . Colloids and Surfaces B , Biointerfaces, 2021 , 208 : 112038 .

EL-NAGGAR M E , ABDEL-ATY A M , WASSEL A R , et al . Immobilization of horseradish peroxidase on cationic microporous starch: physico-bio-chemical characterization and removal of phenolic compounds [J ] . International Journal of Biological Macromolecules , 2021 , 181 : 734 - 742 .

PANTIĆ N , PRODANOVIĆ R , ĐURĐIĆ K I , et al . Optimization of phenol removal with horseradish peroxidase encapsulated within tyramine-alginate micro-beads [J ] . Environmental Technology & Innovation , 2021 , 21 : 101211 .

BILAL M , RASHEED T , IQBAL H M N , et al . Horseradish peroxidase immobilization by copolymerization into cross-linked polyacrylamide gel and its dye degradation and detoxification potential [J ] . International Journal of Biological Macromolecules , 2018 , 113 : 983 - 990 .

WEBER A C , DA SILVA B E , CORDEIRO S G , et al . Immobilization of commercial horseradish peroxidase in calcium alginate-starch hybrid support and its application in the biodegradation of phenol red dye [J ] . International Journal of Biological Macromolecules , 2023 , 246 : 125723 .

PARVEEN S , ASGHER M , BILAL M . Lignin peroxidase-based cross-linked enzyme aggregates (LiP-CLEAs) as robust biocatalytic materials for mitigation of textile dyes-contaminated aqueous solution [J ] . Environmental Technology & Innovation , 2021 , 21 : 101226 .

ALI L , ALGAITHI R , HABIB H M , et al . Soybean peroxidase-mediated degradation of an azo dye-a detailed mechanistic study [J ] . BMC Biochemistry , 2013 , 14 : 35 .

ZHANG Y R , REN J , WANG Q , et al . Oxidation characteristics and degradation potential of a dye-decolorizing peroxidase from Bacillus amyloliquefaciens for crystal violet dye [J ] . Biochemical Engineering Journal , 2021 , 168 : 107930 .

BOUCHERIT N , ABOUSEOUD M , ADOUR L . Degradation of direct azo dye by Cucurbita pepo free and immobilized peroxidase [J ] . Journal of Environmental Sciences , 2013 , 25 ( 6 ): 1235 - 1244 .

WANG H L , LI P , PANG M , et al . Rapid decolourization of azo dyes by a new isolated higher manganese peroxidase producer: Phanerochaete sp. HSD [J ] . Biochemical Engineering Journal , 2009 , 46 ( 3 ): 327 - 333 .

KALSOOM U , ASHRAF S S , MEETANI M A , et al . Mechanistic study of a diazo dye degradation by Soybean Peroxidase [J ] . Chemistry Central Journal , 2013 , 7 ( 1 ): 93 .

PREETHI P S , VICKRAM S , DAS R , et al . Bioprospecting of novel peroxidase from Streptomyces coelicolor strain SPR7 for carcinogenic azo dyes decolorization [J ] . Chemosphere , 2023 , 310 : 136836 .

HAMDI S , ALLALA F , MECHRI S , et al . Biochemical and molecular characterization of a new heme peroxidase from Aspergillus niger CTM10002, and its application in textile reactive dye decolorization [J ] . Process Biochemistry , 2022 , 121 : 619 - 634 .

KALSOOM U , BHATTI H N , AFTAB K , et al . Biocatalytic potential of Brassica oleracea L. var. botrytis leaves peroxidase for efficient degradation of textile dyes in aqueous medium [J ] . Bioprocess and Biosystems Engineering , 2023 , 46 ( 3 ): 453 - 465 .

AI J , ZHANG W J , LIAO G Y , et al . NH 2 Fe 3 O 4 @SiO 2 supported peroxidase catalyzed H 2 O 2 for degradation of endocrine disrupter from aqueous solution: roles of active radicals and NOMs [J ] . Chemosphere , 2017 , 186 : 733 - 742 .

TABOADA-PUIG R , EIBES G , LLORET L , et al . Fostering the action of versatile peroxidase as a highly efficient biocatalyst for the removal of endocrine disrupting compounds [J ] . New Biotechnology , 2016 , 33 ( 1 ): 187 - 195 .

MASHHADI N , TAYLOR K E , JIMENEZ N , et al . Removal of selected pharmaceuticals and personal care products from wastewater using soybean peroxidase [J ] . Environmental Management , 2019 , 63 ( 3 ): 408 - 415 .

AL-MAQDI K A , HISAINDEE S , RAUF M A , et al . Detoxification and degradation of sulfamethoxazole by soybean peroxidase and UV+ H 2 O 2 remediation approaches [J ] . Chemical Engineering Journal , 2018 , 352 : 450 - 458 .

PYLYPCHUK I V , DANIEL G , KESSLER V G , et al . Removal of diclofenac, paracetamol, and carbamazepine from model aqueous solutions by magnetic sol-gel encapsulated horseradish peroxidase and lignin peroxidase composites [J ] . Nanomaterials , 2020 , 10 ( 2 ): 282 .

WEN X H , JIA Y N , LI J X . Enzymatic degradation of tetracycline and oxytetracycline by crude manganese peroxidase prepared from Phanerochaete chrysosporium [J ] . Journal of Hazardous Materials , 2010 , 177 ( 1-3 ): 924 - 928 .

ZDARTA J , DEGÓRSKA O , JANKOWSKA K , et al . Removal of persistent sulfamethoxazole and carbamazepine from water by horseradish peroxidase encapsulated into poly (vinyl chloride) electrospun fibers [J ] . International Journal of Molecular Sciences , 2021 , 23 ( 1 ): 272 .

GARCÍA-ZAMORA J L , LEÓN-AGUIRRE K , QUIROZ-MORALES R , et al . Chloroperoxidase-mediated halogenation of selected pharmaceutical micropollutants [J ] . Catalysts , 2018 , 8 ( 1 ): 32 .

HAMID M , KHALIL-UR-REHMAN . Potential applications of peroxidases [J ] . Food Chemistry , 2009 , 115 ( 4 ): 1177 - 1186 .

LINDGREN A , EMNÉUS J , RUZGAS T , et al . Amperometric detection of phenols using peroxidase-modified graphite electrodes [J ] . Analytica Chimica Acta , 1997 , 347 ( 1-2 ): 51 - 62 .

TKÁC J , STURDÍK E , GEMEINER P . Novel glucose non-interference biosensor for lactose detection based on galactose oxidase-peroxidase with and without co-immobilised β-galactosidase [J ] . The Analyst , 2000 , 125 ( 7 ): 1285 - 1289 .

GILLE D , WALTHER B , BADERTSCHER R , et al . Detection of lactose in products with low lactose content [J ] . International Dairy Journal , 2018 , 83 : 17 - 19 .

LIANG X , CHEN Y X , WEN K , et al . Urate oxidase loaded in PCN-222(Fe) with peroxidase-like activity for colorimetric detection of uric acid [J ] . Journal of Materials Chemistry B , 2021 , 9 ( 34 ): 6811 - 6817 .

AKYILMAZ E , SEZGINTÜRK M K , DINÇKAYA E . A biosensor based on urate oxidase-peroxidase coupled enzyme system for uric acid determination in urine [J ] . Talanta , 2003 , 61 ( 2 ): 73 - 79 .

SAA L , CORONADO-PUCHAU M , PAVLOV V , et al . Enzymatic etching of gold nanorods by horseradish peroxidase and application to blood glucose detection [J ] . Nanoscale , 2014 , 6 ( 13 ): 7405 - 7409 .

CSÖREGI E , GORTON L , MARKO-VARGA G . Amperometric microbiosensors for detection of hydrogen peroxide and glucose based on peroxidase-modified carbon fibers [J ] . Electroanalysis , 1994 , 6 ( 11-12 ): 925 - 933 .

FOSSATI P , PRENCIPE L . Serum triglycerides determined colorimetrically with an enzyme that produces hydrogen peroxide [J ] . Clinical Chemistry , 1982 , 28 ( 10 ): 2077 - 2080 .

PUNDIR C S , AGGARWAL V . Amperometric triglyceride bionanosensor based on nanoparticles of lipase, glycerol kinase, glycerol-3-phosphate oxidase [J ] . Analytical Biochemistry , 2017 , 517 : 56 - 63 .

ZHAO M Y , LI Y , MA X J , et al . Adsorption of cholesterol oxidase and entrapment of horseradish peroxidase in metal-organic frameworks for the colorimetric biosensing of cholesterol [J ] . Talanta , 2019 , 200 : 293 - 299 .

SINGH S , SOLANKI P R , PANDEY M K , et al . Cholesterol biosensor based on cholesterol esterase, cholesterol oxidase and peroxidase immobilized onto conducting polyaniline films [J ] . Sensors and Actuators B: Chemical , 2006 , 115 ( 1 ): 534 - 541 .

NEWMAN D J , CRAGG G M . Natural products as sources of new drugs from 1981 to 2014 [J ] . Journal of Natural Products , 2016 , 79 ( 3 ): 629 - 661 .

PADDON C J , WESTFALL P J , PITERA D J , et al . High-level semi-synthetic production of the potent antimalarial artemisinin [J ] . Nature , 2013 , 496 ( 7446 ): 528 - 532 .

AJIKUMAR P K , XIAO W H , TYO K E , et al . Isoprenoid pathway optimization for Taxol precursor overproduction in Escherichia coli [J ] . Science , 2010 , 330 ( 6000 ): 70 - 74 .

OGUCHI T , TAWAKI S I , UYAMA H , et al . Soluble polyphenol [J ] . Macromolecular rapid communications , 1999 , 20 ( 7 ): 401 - 403 .

UYAMA H , KURIOKA H , SUGIHARA J , et al . Enzymatic synthesis and thermal properties of a new class of polyphenol [J ] . Bulletin of the Chemical Society of Japan , 1996 , 69 ( 1 ): 189 - 193 .

TONAMI H , UYAMA H , KOBAYASHI S , et al . Peroxidase-catalyzed oxidative polymerization of m -substituted phenol derivatives [J ] . Macromolecular Chemistry and Physics , 1999 , 200 ( 10 ): 2365 - 2371 .

DUBEY S , SINGH D , MISRA R A . Enzymatic synthesis and various properties of poly(catechol) [J ] . Enzyme and Microbial Technology , 1998 , 23 ( 7-8 ): 432 - 437 .

TANG B , WANG Y , LIANG H L , et al . Studies on the oxidation reaction of tyrosine (Tyr) with H 2 O 2 catalyzed by horseradish peroxidase (HRP) in alcohol-water medium by spectrofluorimetry and differential spectrophotometry [J ] . Spectrochimica Acta Part A, Molecular and Biomolecular Spectroscopy , 2006 , 63 ( 3 ): 609 - 613 .

SALIU F , TOLPPA E L , ZOIA L , et al . Horseradish peroxidase catalyzed oxidative cross-coupling reactions: the synthesis of ‘unnatural’ dihydrobenzofuran lignans [J ] . Tetrahedron Letters , 2011 , 52 ( 30 ): 3856 - 3860 .

LI C , LU J , XU X F , et al . pH-switched HRP-catalyzed dimerization of resveratrol: a selective biomimetic synthesis [J ] . Green Chemistry , 2012 , 14 ( 12 ): 3281 - 3284 .

RICKLEFS E , GIRHARD M , KOSCHORRECK K , et al . Two-step one-pot synthesis of pinoresinol from eugenol in an enzymatic cascade [J ] . ChemCatChem , 2015 , 7 ( 12 ): 1857 - 1864 .

LÜ Y K , CHENG X Z , DU G C , et al . Engineering of an H 2 O 2 auto-scavenging in vivo cascade for pinoresinol production [J ] . Biotechnology and Bioengineering , 2017 , 114 ( 9 ): 2066 - 2074 .

ABENAVOLI L , CAPASSO R , MILIC N , et al . Milk thistle in liver diseases: past, present, future [J ] . Phytotherapy Research , 2010 , 24 ( 10 ): 1423 - 1432 .

LÜ Y K , XU S , LYU Y B , et al . Engineering enzymatic cascades for the efficient biotransformation of eugenol and taxifolin to silybin and isosilybin [J ] . Green Chemistry , 2019 , 21 ( 7 ): 1660 - 1667 .

YANG J Z , LIANG J C , SHAO L , et al . Green production of silybin and isosilybin by merging metabolic engineering approaches and enzymatic catalysis [J ] . Metabolic Engineering , 2020 , 59 : 44 - 52 .

LI X L , ZHOU Z , LI W N , et al . Design of stable and self-regulated microbial consortia for chemical synthesis [J ] . Nature Communications , 2022 , 13 ( 1 ): 1554 .

PARK S Y , YANG D , HA S H , et al . Production of phenylpropanoids and flavonolignans from glycerol by metabolically engineered Escherichia coli [J ] . Biotechnology and Bioengineering , 2022 , 119 ( 3 ): 946 - 962 .

HABIB M , TRAJKOVIC M , FRAAIJE M W . The biocatalytic synthesis of syringaresinol from 2,6-dimethoxy-4-allylphenol in one-pot using a tailored oxidase/peroxidase system [J ] . ACS Catalysis , 2018 , 8 ( 6 ): 5549 - 5552 .

GUO Y M , ALVIGINI L , SAIFUDDIN M , et al . One-pot biocatalytic synthesis of rac -syringaresinol from a lignin-derived phenol [J ] . ACS Catalysis , 2023 , 13 ( 22 ): 14639 - 14649 .

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