面向高效光驱固碳产醇的蓝细菌合成生物技术研究进展

孙绘梨; 崔金玉; 栾国栋; 吕雪峰

doi:10.12211/2096-8280.2023-051

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面向高效光驱固碳产醇的蓝细菌合成生物技术研究进展

特约评述 | 更新时间：2025-03-20

- 面向高效光驱固碳产醇的蓝细菌合成生物技术研究进展
- Progress of cyanobacterial synthetic biotechnology for efficient light-driven carbon fixation and ethanol production
- 合成生物学 2023年4卷第6期页码：1161-1177
- 作者机构：
  
  1.中国科学院青岛生物能源与过程研究所，中国科学院生物燃料重点实验室，山东青岛 266101
  2.山东能源研究院，山东青岛 266101
  3.青岛新能源山东省实验室，山东青岛 266101
- 作者简介：
  
  [ "孙绘梨（1995—），女，博士后。研究方向为蓝细菌合成生物技术研究，包括光驱固碳细胞工厂的构建和底盘细胞生理功能认识与改造。E-mail：sunhl@qibebt.ac.cn" ]
  [ "吕雪峰（1974—），男，研究员，博士生导师，中国科学院青岛生物能源与过程研究所所长。研究方向为合成生物学与绿色生物制造，在光驱固碳产能蓝细菌的人工设计与构建及真菌天然产物药物等。E-mail：lvxf@qibebt.ac.cn" ]
- 基金信息：
  
  国家重点研发计划(2021YFA0909700);国家自然科学基金(32270103;32070084);山东省博士后创新项目(SDCX-ZG-202202036);中国博士后科学基金第70批面上项目(2021M703320)
- DOI：10.12211/2096-8280.2023-051
  中图分类号： Q815
- 收稿：2023-07-17，
  
  修回：2023-08-24，
  
  纸质出版：2023-12-31
- 稿件说明：
移动端阅览
孙绘梨，崔金玉，栾国栋，吕雪峰. 面向高效光驱固碳产醇的蓝细菌合成生物技术研究进展［J］. 合成生物学， 2023， 4（6）： 1161-1177

SUN Huili， CUI Jinyu， LUAN Guodong， LYU Xuefeng. Progress of cyanobacterial synthetic biotechnology for efficient light-driven carbon fixation and ethanol production［J］. Synthetic Biology Journal， 2023， 4（6）： 1161-1177
孙绘梨，崔金玉，栾国栋，吕雪峰. 面向高效光驱固碳产醇的蓝细菌合成生物技术研究进展［J］. 合成生物学， 2023， 4（6）： 1161-1177 DOI： 10.12211/2096-8280.2023-051.

SUN Huili， CUI Jinyu， LUAN Guodong， LYU Xuefeng. Progress of cyanobacterial synthetic biotechnology for efficient light-driven carbon fixation and ethanol production［J］. Synthetic Biology Journal， 2023， 4（6）： 1161-1177 DOI： 10.12211/2096-8280.2023-051.

摘要

蓝细菌能够直接利用二氧化碳和太阳能通过光合作用生产乙醇，为提供绿色生物燃料提供了一条有前途的可持续路线。光驱固碳合成乙醇是最具代表性的蓝细菌光合生物制造技术。乙醇并不属于典型的蓝细菌天然代谢物，蓝细菌产醇细胞工厂的构建需要通过向基因组中导入异源的丙酮酸脱羧酶并结合异源/内源醇脱氢酶的过量表达来实现；在过去二十多年间，通过蛋白、途径、底盘、工艺层面的系统优化，蓝细菌产醇细胞工厂的效能得到有效提高，乙醇成为目前产量最高、产率最高、碳流分配率最高的蓝细菌代谢工程产物。本文总结并比较了“蓝细菌生物质炼制产醇”“蓝细菌固碳产糖-产醇”“蓝细菌固碳直接产醇”等三种光驱固碳产醇技术路线，并以构建光合细胞工厂驱动二氧化碳一站式转化为乙醇的技术路线为主，从乙醇合成途径优化与强化、蓝细菌光合碳代谢网络的调节与重塑、代谢网络模型与计算机辅助设计引导细胞工厂构建和优化三个方面对蓝细菌高效光驱固碳合成乙醇的技术发展历程和基本现状进行了介绍，特别是强调了计算生物学、系统代谢工程、生物材料嵌合等研究手段在本领域的应用进展；在此基础上，也从新型底盘开发、高通量筛选技术应用、细胞工厂的稳定性与鲁棒性优化等角度对蓝细菌固碳产醇的未来发展方向进行了展望。

Abstract

The utilization of solar energy and carbon dioxide by cyanobacterial cell factories for photosynthetic ethanol production represents a promising and sustainable route towards green biofuels. Ethanol is one of the most representative products of the cyanobacterial photosynthetic biomanufacturing technology. Cyanobacterial ethanol production systems could serve as models for developing and optimizing advanced synthetic biology and metabolic engineering strategies. While most known cyanobacterial species lack the ability to synthesize and accumulate ethanol

the introduction and overexpression of heterologous pyruvate decarboxylase and alcohol dehydrogenase (heterologous or native) are required to enable ethanol synthesis in cyanobacteria. In the past decades

the performance of ethanol-producing cyanobacterial cell factories has been significantly improved through systematic optimization of proteins

pathways

chassis cells

and cultivation techniques. Cyanobacterial ethanol production technology has yielded the highest titer

productivity

and carbon partitioning ratio among all current cyanobacterial biomanufacturing systems. Recent advances have led to further improved efficiency of cyanobacterial ethanol photosynthetic production. Based on extensive systems biology data and rapidly developing computer modeling technologies

more accurate simulations of cyanobacterial physiological characteristics and metabolic networks have become possible. These simulations facilitate the identification of potential modification targets

thereby enhancing ethanol production capacity and guiding the design of next-generation alcohol-producing cell factories. With a more comprehensive understanding of cyanobacteria physiology and metabolism

systematic genome modifications and pathway optimizations have been performed

resulting in further improved ethanol productivity and final titers. Concurrently

efforts have been made to improve model strains and evaluate newly emerging non-conventional strains to establish more robust and efficient ethanol production processes. In conclusion

this review summarizes and compares three technological routes of light-driven carbon fixation and ethanol production in cyanobacteria

introduces the technological development trajectory and basic status of efficient light-driven carbon fixation for ethanol synthesis by cyanobacteria

provides valuable and up-to-date insights to facilitate the development of more promising cyanobacterial ethanol photosynthetic production technologies and explores future challenges and directions in this dynamic field.

关键词

Keywords

references

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