热纤梭菌在生物质能源开发中的合成生物学研究进展

肖艳; 刘亚君; 冯银刚; 崔球

doi:10.12211/2096-8280.2023-046

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热纤梭菌在生物质能源开发中的合成生物学研究进展

特约评述 | 更新时间：2025-03-20

- 热纤梭菌在生物质能源开发中的合成生物学研究进展
- Progress in synthetic biology research of Clostridium thermocellum for biomass energy applications
- 合成生物学 2023年4卷第6期页码：1055-1081
- 作者机构：
  
  1.中国科学院青岛生物能源与过程研究所，中国科学院生物燃料重点实验室，山东省合成生物学重点实验室，山东青岛 266101
  2.山东省能源研究院，山东青岛 266101
  3.青岛新能源山东省实验室，山东青岛 266101
  4.中国科学院大学，北京 100049
- 作者简介：
  
  [ "肖艳（1982—），女，博士，副研究员，硕士生导师。研究方向为能源微生物代谢机理与改造。E-mail：xiaoyan@qibebt.ac.cn" ]
  [ "冯银刚（1977—），男，博士，研究员，博士生导师。研究方向为能源微生物的分子生理机制与合成生物学应用、工业酶催化机制与酶工程等。E-mail：fengyg@qibebt.ac.cn" ]
  [ "崔球（1975—），男，博士，研究员，博士生导师。研究方向为基于代谢物组/代谢流组学的计算分析辅助能源微生物代谢工程设计、蛋白质结构功能研究。E-mail：cuiqiu@qibebt.ac.cn" ]
- 基金信息：
  
  国家自然科学基金(32070125;32170051);山东能源研究院(SEI I202106;SEI S202106);山东省自然科学基金(ZR2022MC128)
- DOI：10.12211/2096-8280.2023-046
  中图分类号： Q812
- 收稿：2023-07-02，
  
  修回：2023-09-22，
  
  纸质出版：2023-12-31
- 稿件说明：
移动端阅览
肖艳，刘亚君，冯银刚，崔球. 热纤梭菌在生物质能源开发中的合成生物学研究进展［J］. 合成生物学， 2023， 4（6）： 1055-1081

XIAO Yan， LIU Yajun， FENG Yin′gang， CUI Qiu. Progress in synthetic biology research of Clostridium thermocellum for biomass energy applications［J］. Synthetic Biology Journal， 2023， 4（6）： 1055-1081
肖艳，刘亚君，冯银刚，崔球. 热纤梭菌在生物质能源开发中的合成生物学研究进展［J］. 合成生物学， 2023， 4（6）： 1055-1081 DOI： 10.12211/2096-8280.2023-046.

XIAO Yan， LIU Yajun， FENG Yin′gang， CUI Qiu. Progress in synthetic biology research of Clostridium thermocellum for biomass energy applications［J］. Synthetic Biology Journal， 2023， 4（6）： 1055-1081 DOI： 10.12211/2096-8280.2023-046.

摘要

农林废弃物、能源植物、微藻等生物质是唯一同时具备“能源”和“物质”双重属性的可再生资源，在替代不可再生的化石能源方面具有巨大的潜力。木质纤维素生物转化的核心之一在于高效生物催化剂的构建。热纤梭菌是高效降解木质纤维素的嗜热厌氧菌，是多种木质纤维素生物转化策略的理想底盘菌株，在生物质能源开发中具有重要价值。经过近二十年的研究和开发，针对热纤梭菌已经建立了多种遗传改造技术，并构建了可以生产多种能源分子及化学品的热纤梭菌细胞工厂。本文首先介绍了热纤梭菌及其纤维素降解与利用特性，简述了热纤梭菌的系统生物学研究和遗传改造工具开发的现状，随后重点回顾和总结了热纤梭菌在生产乙醇、丁醇、异丁醇、氢气、乳酸、中/短链脂肪酸酯和可发酵糖等生物能源开发中的合成生物学研究进展。最后对热纤梭菌的合成生物学发展方向进行了展望，并强调了合成生物学技术在未来生物质能源开发中的重要作用。

Abstract

Biomass

including agricultural and forestry waste

energy plants

and microalgae

possesses both "energy" and "substance" properties

making it a promising renewable resource that can potentially replace fossil fuels. The efficient lignocellulose bioconversion relies on the development of effective biocatalysts.

Clostridium thermocellum

(also known as

Ruminiclostridium thermocellum

Hungateiclostridium thermocellum

and

Acetivibrio thermocellus

) is a thermophilic anaerobic bacterium that can efficiently degrade lignocellulosic biomass. Over the past two decades

extensive research and development have led to the potential of using

C. thermocellum

as a cell factory to produce various energy and chemicals from lignocellulose.

C. thermocellum

has been used to produce ethanol

butanol

isobutanol

hydrogen

lactic acid

medium/short-chain fatty acid esters

and fermentable sugars from lignocellulosic biomass. The degradation and utilization process of lignocellulosic biomass by

C. thermocellum

mainly involves substrate recognition and hydrolysis through the cellulosome

hydrolysate uptake through ABC transporters

and intracellular metabolism

via

atypical glycolytic pathways.

C. thermocellum

possesses dynamic regulation of cellulosome production adapting extracellular substrates

which enables the

high capability of degrading various lignocellulosic substrates. The cellulosome consists of non-catalytic scaffoldins and multiple enzymatic subunits with distinct catalytic activities and has broad applications in synthetic biology as well as lignocellulose degradation. In addition to lignocellulose refinery

the thermophilic

C. thermocellum

also has great potential in synthetic biology research under high-temperature conditions. Several genetic manipulation tools have been developed for

C. thermocellum

although greater challenges have been encountered compared to model organisms such as

Escherichia coli

. The genetic tools include homologous recombination technology

Thermotargetron technology

and CRISPR/Cas systems

which enable gene knockout

insertion

replacement

mutation

and expression regulation of target genes in the strain.

C. thermocellum

has been used as the whole-cell biocatalyst for lignocellulose bioconversion through consolidated bioprocessing (CBP) and consolidated bio-saccharification (CBS). CBS follows the concept of sugar platform construction and shows great potential in real-world applications. The synthetic biology research targeting the CBS strategy still requires future development. For example

we need to explore new genetic tools and thermophilic functional elements for

C. thermocellum

and improve the efficiency of gene editing. We need to st

rengthen research on the genetic

physiological

and metabolic aspects of

C. thermocellum

and the molecular mechanisms underlying lignocellulose degradation. It is noteworthy that

as a strict anaerobe

C. thermocellum

cannot be used as the chassis for catalyzing oxygen-involved reactions. Selecting suitable metabolic pathways and target products will be the focus in future developments of synthetic biology based on

C. thermocellum

. Therefore

we need to investigate additional target pathways and products for synthetic biology development. In recent years

automation methods and artificial intelligence (AI) technologies are being developed rapidly and have been applied in various synthetic biology research fields. Such technologies may also be employed to promote research on thermophilic and anaerobic microorganisms.

关键词

Keywords

references

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