生物设施平台及其工业应用

赵国淼; 杨鑫; 张媛; 王靖; 谭剑; 魏超; 周娜娜; 李凡; 王小艳

doi:10.12211/2096-8280.2023-024

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生物设施平台及其工业应用

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- 生物设施平台及其工业应用
- Biofoundry and its industrial application
- 合成生物学 2023年4卷第5期页码：892-903
- 作者机构：
  
  1.中粮营养健康研究院有限公司，北京 102209
  2.营养健康与食品安全北京市重点实验室，北京 102209
- 作者简介：
  
  [ "赵国淼（1989—），男，博士，工程师。研究方向为工业微生物改造与高通量筛选、计算生物学与生物信息学。E-mail：zhaoguomiao@cofco.com" ]
  [ "王小艳（1980—），女，博士，正高级工程师。研究方向围绕淀粉质原料生物加工过程工业菌株和酶制剂的开发，主要聚焦生物燃料乙醇、生物基材料、功能糖等领域。E-mail：wangxiaoyan@cofco.com" ]
- 基金信息：
  
  国家重点研发计划“合成生物学”重点专项(2018YFA0902900)
- DOI：10.12211/2096-8280.2023-024
  中图分类号： Q819
- 收稿：2023-03-17，
  
  修回：2023-05-08，
  
  纸质出版：2023-10-31
- 稿件说明：
移动端阅览
赵国淼，杨鑫，张媛，王靖，谭剑，魏超，周娜娜，李凡，王小艳. 生物设施平台及其工业应用［J］. 合成生物学， 2023， 4（5）： 892-903

ZHAO Guomiao， YANG Xin， ZHANG Yuan， WANG Jing， TAN Jian， WEI Chao， ZHOU Nana， LI Fan， WANG Xiaoyan. Biofoundry and its industrial application［J］. Synthetic Biology Journal， 2023， 4（5）： 892-903
赵国淼，杨鑫，张媛，王靖，谭剑，魏超，周娜娜，李凡，王小艳. 生物设施平台及其工业应用［J］. 合成生物学， 2023， 4（5）： 892-903 DOI： 10.12211/2096-8280.2023-024.

ZHAO Guomiao， YANG Xin， ZHANG Yuan， WANG Jing， TAN Jian， WEI Chao， ZHOU Nana， LI Fan， WANG Xiaoyan. Biofoundry and its industrial application［J］. Synthetic Biology Journal， 2023， 4（5）： 892-903 DOI： 10.12211/2096-8280.2023-024.

摘要

传统菌株改造和筛选实验存在操作烦琐、耗时、易错、难以规模化等问题，生物设施平台将自动化、机器人技术、数据分析与生物研究相结合，通过导轨和机械手臂实现自动化操作，提高了实验操作的稳定性，通过缩小培养体积（微孔板或微液滴），提高了培养和筛选通量，解决了上述问题，大大提高了研发效率。本文简单介绍了自动化设施平台的发展和常见的高通量检测方法，重点介绍了中粮营养健康研究院的自动化设施平台，并结合开展的项目叙述了平台在生物燃料菌株开发、传统酿造菌株筛选、酶的定向进化和筛选等领域的应用，可以预见自动化和高通量化在菌株改造和筛选方向巨大的应用价值。实验室自动化是涉及机械工程、自动化、计算机和生命科学等学科的交叉领域，需要各方面共同努力，才能推动实验室向更高程度的自动化和智能化方向发展。

Abstract

Traditional methods for strain isolation and improvement can be cumbersome

time-consuming

error-prone

and difficult to scale up

leading to inefficiencies in research and development workflows. With the integration of automation and robotics technology

biofoundry can achieve automated operations through guide rails and robotic arms

leading to improved stability and precision of experimental operations. Additionally

by utilizing smaller cultivation volumes

such as microplates or droplets

the cultivation and screening throughput can be increased

addressing the currently existing issues of traditional methods. This can greatly improve research and development efficiency

allowing for the testing and optimization of large numbers of microbial strains or genetic variants in a high-throughput manner. The biofoundry encompasses interdisciplinary fields such as mechanical engineering

automation

computer science

and life sciences. The collaboration among these fields is crucial for the development and advancement of laboratory automation. By leveraging automation and high-throughput technologies

the field of strain isolation and improvement can benefit from increased efficiency

improved reliability

and scalability. These advancements can accelerate the progress of microbial strain engineering for various applications in biotechnology

medicine

agriculture

and energy production. This paper briefly introduces the composition and classification of the biofoundry

the high-throughput detection method

and focuses on the high-throughput screening platform built by the research team from Nutrition & Health Research Institute

COFCO. In combination with the projects carried out

it introduces the application of the high-throughput screening platform in the fields of biofuel strain development

traditional brewing strain screening

feed substitute antimicrobial screening

directed evolution and screening of enzymes

etc.

关键词

Keywords

references

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