面向自动化铸造平台的多功能微孔板检测系统

马翠; 杨凡; 张君泰; 何凯

doi:10.12211/2096-8280.2023-023

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面向自动化铸造平台的多功能微孔板检测系统

研究论文 | 更新时间：2025-03-20

- 面向自动化铸造平台的多功能微孔板检测系统
- Multifunction microplate reader for automated foundry platform
- 合成生物学 2023年4卷第5期页码：1036-1049
- 作者机构：
  
  1.中国科学院深圳先进技术研究院，广东　深圳　518055
  2.深圳市精密工程重点实验室，广东　深圳　518055
- 作者简介：
  
  [ "马翠（1983—），女，助理研究员。研究方向为光学检测。E-mail：cui.ma@siat.ac.cn" ]
  [ "何凯（1972—），男，博士，教授级高级工程师。研究方向包括精密制造及自动化技术、工业机器人、金属成形技术及装备、生物制造自动化技术等。E-mail：kai.he@siat.ac.cn" ]
- 基金信息：
  
  国家重点研发计划(2018YFA0902904)
- DOI：10.12211/2096-8280.2023-023
  中图分类号： TH773;Q819
- 收稿：2023-03-16，
  
  修回：2023-07-11，
  
  纸质出版：2023-10-31
- 稿件说明：
移动端阅览
马翠，杨凡，张君泰，何凯. 面向自动化铸造平台的多功能微孔板检测系统［J］. 合成生物学， 2023， 4（5）： 1036-1049

MA Cui， YANG Fan， ZHANG Juntai， HE Kai. Multifunction microplate reader for automated foundry platform［J］. Synthetic Biology Journal， 2023， 4（5）： 1036-1049
马翠，杨凡，张君泰，何凯. 面向自动化铸造平台的多功能微孔板检测系统［J］. 合成生物学， 2023， 4（5）： 1036-1049 DOI： 10.12211/2096-8280.2023-023.

MA Cui， YANG Fan， ZHANG Juntai， HE Kai. Multifunction microplate reader for automated foundry platform［J］. Synthetic Biology Journal， 2023， 4（5）： 1036-1049 DOI： 10.12211/2096-8280.2023-023.

摘要

生物分析技术正向高通量、高灵敏度、多功能方向发展，微孔板检测仪作为高通量分析的基础仪器，需要满足微量化、自动化、集成化的要求。目前国内单一功能的微孔板检测仪器技术成熟，检测精度已与国际水平相当，但多功能的微孔板检测仪还较少，大多使用进口仪器。进口产品价格昂贵，有些操作指令不公开，不易集成到自主研发的合成生物自动化铸造平台项目。本研究将对高精度吸光度检测和高灵敏度荧光检测等关键技术进行研究，开发具有吸光检测和荧光检测功能的微孔板检测仪，并自主开发控制系统及数据采集分析软件，使其易于接入自动化铸造平台。该微孔板检测仪结构紧凑，吸光检测和荧光检测模块独立，波段可扩展。通过实验测试，所研发的微孔板检测仪的吸光度检测重复性为0.3%，10 s内可完成96孔的吸光检测；荧光检测数据在皮摩尔浓度范围内线性度较好，对荧光素钠溶液的检测灵敏度大约3.9 pmol/L。此外，该系统可通过串口直接连接自动化平台集成控制系统，也可通过TCP方式进行远程通信控制。

Abstract

Bioanalytical technology is being developed towards the direction of high throughput

high sensitivity and multi-functionality. Microplate readers serve as fundamental instruments for high-throughput analysis. They need to meet the requirements of microanalysis

automation and integration. At present

domestic instruments have limitations in terms of functionality and automation. Although the single-function microplate reader has basically reached the level of oversea equivalents

there is still a gap in the multifunctional instruments. Most of the multi-function microplate reader used are imported instruments. They are usually expensive and some operating instructions are not fully disclosed. It is difficult to integrate them into the automated foundry platform. In this study

we will research key technologies of high-precision absorbance detection and high-sensitivity fluorescence detection

and develop a multifunction microplate reader. The control and soft system is also developed independently to access the automated foundry platform. In our system

the absorbance optical system includes light source

filters

fibers and lens array. Multiple channels of transmitted light are detected in parallel to improve the speed of detection. Combined with the one-dimensional movement of microplate

the whole 96 wells are measured. The fluorescence optical system mainly includes LED light source

filter cube (emission filter

excitation filter and dichroic mirror) and photodiode detector. Single channel of excitation light is detected and two-dimension scanning mechanism is realized for the whole microplate measurement. Besides the optical system

high precision data acquisition system is developed

including signal amplifying

conditioning and isolating circuit. This microplate reader is compact with independent absorbance and fluorescence modules

and scalable wavebands. According to experimental results

the reproducibility of absorbance measurement is high with a standard error of 0.3% and the total 96 micro-wells can be detected accurately within 10 seconds. The fluorescence detection has good linearity in the picomole concentration and the limit of detection for sodium fluorescein is about 3.9 pmol/L. For synthetic biology automated foundry platform

this microplate reader can be directly connected to the integration control system through serial port

and can also be remotely controlled by TCP communication.

关键词

Keywords

references

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