重组胶原蛋白表达体系研究进展

潘家豪; 潘炜松; 邱健; 谢东玲; 邹奇; 吴川

doi:10.12211/2096-8280.2023-020

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重组胶原蛋白表达体系研究进展

特约评述 | 更新时间：2025-03-20

- 重组胶原蛋白表达体系研究进展
- Research progress on recombinant collagen expression system
- 合成生物学 2023年4卷第4期页码：808-823
- 作者机构：
  
  1.中南大学冶金与环境学院，湖南长沙 410083
  2.湖南诺合新生物科技有限公司，湖南长沙 410001
  3.中南大学湘雅医院，湖南长沙 410008
  4.华南师范大学生命科学学院，广东广州 510631
- 作者简介：
  
  [ "潘家豪（2000—），男，硕士研究生。研究方向为重组胶原蛋白在植物表达及其应用。E-mail：thekid123go@163.com" ]
  [ "吴川（1983—），女，教授，博士生导师，湖湘青年英才，香港裘槎访问学者。研究方向为植物生物反应器、重金属污染生物修复等方面的研究。E-mail：wuchuan@csu.edu.cn" ]
- 基金信息：
  
  国家自然科学基金(31670955);中南大学前沿交叉项目(2023QYJC035)
- DOI：10.12211/2096-8280.2023-020
  中图分类号： Q816
- 收稿：2023-03-06，
  
  修回：2023-05-17，
  
  纸质出版：2023-08-31
- 稿件说明：
移动端阅览
潘家豪，潘炜松，邱健，谢东玲，邹奇，吴川. 重组胶原蛋白表达体系研究进展［J］. 合成生物学， 2023， 4（4）： 808-823

PAN Jiahao， PAN Weisong， QIU Jian， XIE Donling， ZOU Qi， WU Chuan. Research progress on recombinant collagen expression system［J］. Synthetic Biology Journal， 2023， 4（4）： 808-823
潘家豪，潘炜松，邱健，谢东玲，邹奇，吴川. 重组胶原蛋白表达体系研究进展［J］. 合成生物学， 2023， 4（4）： 808-823 DOI： 10.12211/2096-8280.2023-020.

PAN Jiahao， PAN Weisong， QIU Jian， XIE Donling， ZOU Qi， WU Chuan. Research progress on recombinant collagen expression system［J］. Synthetic Biology Journal， 2023， 4（4）： 808-823 DOI： 10.12211/2096-8280.2023-020.

摘要

胶原蛋白是哺乳动物中含量最多的蛋白质，至今已发现28种类型，主要分为纤维性胶原蛋白、网状胶原蛋白、珠状丝状胶原蛋白、锚定纤维蛋白、膜蛋白以及multiplexins胶原蛋白，其中纤维性胶原蛋白中Ⅰ型、Ⅱ型、Ⅲ型胶原蛋白占人体胶原蛋白的80%~90%。目前，根据来源，胶原蛋白大致分为动物源胶原蛋白和重组胶原蛋白。动物源胶原蛋白主要来源于陆生动物以及海洋动物，而重组胶原蛋白是指将人胶原蛋白基因克隆到选定的表达载体并转化到表达细胞内，最后通过纯化技术所获得的蛋白质。本文简述了胶原蛋白的结构、类别和生物合成机制，重点阐述了重组胶原蛋白表达体系及特点，包括原核生物、酵母、植物、杆状病毒以及哺乳动物细胞等表达体系及其优势与局限性，介绍了重组胶原蛋白市场前景及在眼科、软骨工程、皮肤治疗等生物医药方面的实际应用，并对重组胶原蛋白的研究和产业发展进行了展望。

Abstract

Collagen is the most abundant protein in mammals

and its production has been widely used in biomedicine

cosmetics

leather

biotechnology

etc. At present

collagen is generally divided into animal collagen and recombinant collagen. Although animal collagen is the main source of collagen

most of it comes from animal carcasses

and its collagen has been cross-linked and embedded in native tissues

which is more demanding on extraction and purification technology. In addition

pathogen contamination and allergy risks have become unavoidable problems for animal collagen. Recombinant collagen is a protein obtained by using human collagen cDNA fragments as the backbone gene

cloning the gene to the selected expression vector and converting it into an expression cell

and finally achieved by purification technology. Due to its single molecule

clear structure and easy control

recombinant collagen is the best alternative to replace animal collagen in biomedicine and tissue engineering. In this paper

the structure

关键词

Keywords

references

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