HÜTTNER S , JOHANSSON A , GONÇALVES TEIXEIRA P , et al . Recent advances in the intellectual property landscape of filamentous fungi [J ] . Fungal Biology and Biotechnology , 2020 , 7 ( 1 ): 16 .

EVANS D A , BEIGER J J , BURCH J D , et al . Total synthesis of aflastatin A [J ] . Journal of the American Chemical Society , 2022 , 144 ( 43 ): 19953 - 19972 .

PÉREZ-PÉREZ W D , CARRASCO-NAVARRO U , GARCÍA-ESTRADA C , et al . bZIP transcription factors PcYap1 and PcRsmA link oxidative stress response to secondary metabolism and development in Penicillium chrysogenum [J ] . Microbial Cell Factories , 2022 , 21 ( 1 ): 50 .

LIU L , CHEN Z , LIU W Y , et al . Cephalosporin C biosynthesis and fermentation in Acremonium chrysogenum [J ] . Applied Microbiology and Biotechnology , 2022 , 106 ( 19 ): 6413 - 6426 .

YAO G S , BAI X F , ZHANG B X , et al . Enhanced production of terrein in marine-derived Aspergillus terreus by refactoring both global and pathway-specific transcription factors [J ] . Microbial Cell Factories , 2022 , 21 ( 1 ): 136 .

GUO W Z , YANG J H , HUANG T C , et al . Synergistic effects of multiple enzymes from industrial Aspergillus niger strain O1 on starch saccharification [J ] . Biotechnology for Biofuels , 2021 , 14 ( 1 ): 225 .

AHMED T , RANA M R , ZZAMAN W , et al . Optimization of substrate composition for pectinase production from Satkara ( Citrus macroptera ) peel using Aspergillus niger -ATCC 1640 in solid-state fermentation [J ] . Heliyon , 2021 , 7 ( 10 ): e08133 .

WANG Y , CHEN H Y , MA L , et al . Use of CRISPR-Cas tools to engineer Trichoderma species [J ] . Microbial Biotechnology , 2022 , 15 ( 10 ): 2521 - 2532 .

SCHALÉN M , ANYAOGU D C , HOOF J B , et al . Effect of secretory pathway gene overexpression on secretion of a fluorescent reporter protein in Aspergillus nidulans [J ] . Fungal Biology and Biotechnology , 2016 , 3 : 3 .

SCHÜLLER A , STUDT-REINHOLD L , STRAUSS J . How to completely squeeze a fungus—advanced genome mining tools for novel bioactive substances [J ] . Pharmaceutics , 2022 , 14 ( 9 ): 1837 .

TANG S , MEN P , ZHANG W , et al . Identification of a polyketide biosynthesis gene cluster by transcriptional regulator activation in Aspergillus terreus [J ] . Fungal Genetics and Biology , 2022 , 160 : 103690 .

ZHOU Y , WANG Y L , CHEN K , et al . Near-complete genomes of two Trichoderma species: a resource for biological control of plant pathogens [J ] . Molecular Plant-Microbe Interactions , 2020 , 33 ( 8 ): 1036 - 1039 .

ZOU G , NIELSEN J B , WEI Y J . Harnessing synthetic biology for mushroom farming [J ] . Trends in Biotechnology , 2023 , 41 ( 4 ): 480 - 483 .

WRIGHT A V , NUÑEZ J K , DOUDNA J A . Biology and applications of CRISPR systems: Harnessing nature's toolbox for genome engineering [J ] . Cell , 2016 , 164 ( 1/2 ): 29 - 44 .

HUANG X N , MEN P , TANG S , et al . Aspergillus terreus as an industrial filamentous fungus for pharmaceutical biotechnology [J ] . Current Opinion in Biotechnology , 2021 , 69 : 273 - 280 .

CUI Z F , LIU H , ZHANG H F , et al . The comparison of ZFNs, TALENs, and Sp Cas9 by GUIDE-seq in HPV-targeted gene therapy [J ] . Molecular Therapy Nucleic Acids , 2021 , 26 : 1466 - 1478 .

BARRANGOU R , DOUDNA J A . Applications of CRISPR technologies in research and beyond [J ] . Nature Biotechnology , 2016 , 34 ( 9 ): 933 - 941 .

DOUDNA J A . The promise and challenge of therapeutic genome editing [J ] . Nature , 2020 , 578 ( 7794 ): 229 - 236 .

VALENTE S , COMETTO A , PIOMBO E , et al . Elaborated regulation of griseofulvin biosynthesis in Penicillium griseofulvum and its role on conidiation and virulence [J ] . International Journal of Food Microbiology , 2020 , 328 : 108687 .

HOFFMEISTER D , KELLER N P . Natural products of filamentous fungi: enzymes, genes, and their regulation [J ] . Natural Product Reports , 2007 , 24 ( 2 ): 393 - 416 .

刘佳佳 , 刘钢 . 头孢菌素C生物合成调控研究进展 [J ] . 微生物学报 , 2016 , 56 ( 3 ): 461 - 470 .

LIU J J , LIU G . Advances in the regulation of cephalosporin C biosynthesis [J ] . Acta Microbiologica Sinica , 2016 , 56 ( 3 ): 461 - 470 .

黄润业 , 亓兰达 , 陈国参 , 等 . 免疫抑制剂霉酚酸的研究及产业化进展 [J ] . 微生物学报 , 2021 , 61 ( 10 ): 3010 - 3025 .

HUANG R Y , QI L D , CHEN G C , et al . Research and industrialization progress of immunosuppressant mycophenolic acid [J ] . Acta Microbiologica Sinica , 2021 , 61 ( 10 ): 3010 - 3025 .

黄雪年 , 唐慎 , 吕雪峰 . 工业丝状真菌土曲霉合成生物技术研究进展及展望 [J ] . 合成生物学 , 2020 , 1 ( 2 ): 187 - 211 .

HUANG X N , TANG S , LV X F . Progress and prospect for synthetic biology research of the industrial filamentous fungi Aspergillus terreus [J ] . Synthetic Biology Journal , 2020 , 1 ( 2 ): 187 - 211 .

RAJA H A , MILLER A N , PEARCE C J , et al . Fungal identification using molecular tools: a primer for the natural products research community [J ] . Journal of Natural Products , 2017 , 80 ( 3 ): 756 - 770 .

WU Q W , LI M Z , BILAL M , et al . Enhanced production of mycophenolic acid from Penicillium brevicompactum via optimized fermentation strategy [J ] . Applied Biochemistry and Biotechnology , 2022 , 194 ( 7 ): 3001 - 3015 .

ISTVAN E S , DEISENHOFER J . Structural mechanism for statin inhibition of HMG-CoA reductase [J ] . Science , 2001 , 292 ( 5519 ): 1160 - 1164 .

HUANG X N , LIANG Y J , YANG Y , et al . Single-step production of the simvastatin precursor monacolin J by engineering of an industrial strain of Aspergillus terreus [J ] . Metabolic Engineering , 2017 , 42 : 109 - 114 .

HUANG X N , TANG S , ZHENG L H , et al . Construction of an efficient and robust Aspergillus terreus cell factory for monacolin J production [J ] . ACS Synthetic Biology , 2019 , 8 ( 4 ): 818 - 825 .

KELLER N P . Fungal secondary metabolism: regulation, function and drug discovery [J ] . Nature Reviews Microbiology , 2019 , 17 ( 3 ): 167 - 180 .

LAZARUS C M , WILLIAMS K , BAILEY A M . Reconstructing fungal natural product biosynthetic pathways [J ] . Natural Product Reports , 2014 , 31 ( 10 ): 1339 - 1347 .

ZHANG R K , TAN Y S , CUI Y Z , et al . Lignin valorization for protocatechuic acid production in engineered Saccharomyces cerevisiae [J ] . Green Chemistry , 2021 , 23 ( 17 ): 6515 - 6526 .

RO D K , PARADISE E M , OUELLET M , et al . Production of the antimalarial drug precursor artemisinic acid in engineered yeast [J ] . Nature , 2006 , 440 ( 7086 ): 940 - 943 .

WANG P P , WEI W , YE W , et al . Synthesizing ginsenoside Rh2 in Saccharomyces cerevisiae cell factory at high-efficiency [J ] . Cell Discovery , 2019 , 5 : 5 .

ZHANG J , HANSEN L G , GUDICH O , et al . A microbial supply chain for production of the anti-cancer drug vinblastine [J ] . Nature , 2022 , 609 ( 7926 ): 341 - 347 .

CONG L , RAN F A , COX D , et al . Multiplex genome engineering using CRISPR/Cas systems [J ] . Science , 2013 , 339 ( 6121 ): 819 - 823 .

YANG W , YAN J Q , ZHUANG P Z , et al . Progress of delivery methods for CRISPR-Cas9 [J ] . Expert Opinion on Drug Delivery , 2022 , 19 ( 8 ): 913 - 926 .

DICARLO J E , NORVILLE J E , MALI P , et al . Genome engineering in Saccharomyces cerevisiae using CRISPR-Cas systems [J ] . Nucleic Acids Research , 2013 , 41 ( 7 ): 4336 - 4343 .

LIU R , CHEN L , JIANG Y P , et al . Efficient genome editing in filamentous fungus Trichoderma reesei using the CRISPR/Cas9 system [J ] . Cell Discovery , 2015 , 1 : 15007 .

ZHU Y M . Advances in CRISPR/Cas9 [J ] . BioMed Research International , 2022 , 2022 : 9978571 .

FAN C , ZHANG W , SU X Y , et al . CRISPR/Cas9-mediated genome editing directed by a 5S rRNA-tRNA Gly hybrid promoter in the thermophilic filamentous fungus Humicola insolens [J ] . Biotechnology for Biofuels , 2021 , 14 ( 1 ): 206 .

GHOSH D , RAGHAVAN S C . Nonhomologous end joining: new accessory factors fine tune the machinery [J ] . Trends in Genetics , 2021 , 37 ( 6 ): 582 - 599 .

SUZUKI K , TSUNEKAWA Y , HERNANDEZ-BENITEZ R , et al . In vivo genome editing via CRISPR/Cas9 mediated homology-independent targeted integration [J ] . Nature , 2016 , 540 ( 7631 ): 144 - 149 .

CLEMMENSEN S E , KROMPHARDT K K , FRANDSEN R N . Marker-free CRISPR-Cas9 based genetic engineering of the phytopathogenic fungus, Penicillium expansum [J ] . Fungal Genetics and Biology , 2022 , 160 : 103689 .

NØDVIG C S , NIELSEN J B , KOGLE M E , et al . A CRISPR-Cas9 system for genetic engineering of filamentous fungi [J ] . PLoS One , 2015 , 10 ( 7 ): e0133085 .

SHOJAEI BAGHINI S , GARDANOVA Z R , ABADI S A H , et al . CRISPR/Cas9 application in cancer therapy: a pioneering genome editing tool [J ] . Cellular & Molecular Biology Letters , 2022 , 27 ( 1 ): 35 .

LI J , ZHANG L Y , XU Q , et al . CRISPR-Cas9 toolkit for genome editing in an autotrophic CO 2 -fixing methanogenic archaeon [J ] . Microbiology Spectrum , 2022 , 10 ( 4 ): e0116522 .

DARMA R , LUTZ A , ELLIOTT C E , et al . Identification of a gene cluster for the synthesis of the plant hormone abscisic acid in the plant pathogen Leptosphaeria maculans [J ] . Fungal Genetics and Biology: , 2019 , 130 : 62 - 71 .

WANG Q , COBINE P A , COLEMAN J J . Efficient genome editing in Fusarium oxysporum based on CRISPR/Cas9 ribonucleoprotein complexes [J ] . Fungal Genetics and Biology , 2018 , 117 : 21 - 29 .

SHI T Q , GAO J , WANG W J , et al . CRISPR/Cas9-based genome editing in the filamentous fungus Fusarium fujikuroi and its application in strain engineering for gibberellic acid production [J ] . ACS Synthetic Biology , 2019 , 8 ( 2 ): 445 - 454 .

REN C , LIU Y F , GUO Y C , et al . Optimizing the CRISPR/Cas9 system for genome editing in grape by using grape promoters [J ] . Horticulture Research , 2021 , 8 : 52 .

DENG H X , GAO R J , LIAO X R , et al . Genome editing in Shiraia bambusicola using CRISPR-Cas9 system [J ] . Journal of Biotechnology , 2017 , 259 : 228 - 234 .

CHEN J J , LAI Y L , WANG L L , et al . CRISPR/Cas9-mediated efficient genome editing via blastospore-based transformation in entomopathogenic fungus Beauveria bassiana [J ] . Scientific Reports , 2017 , 7 : 45763 .

LEYNAUD-KIEFFER L M C , CURRAN S C , KIM I , et al . A new approach to Cas9-based genome editing in Aspergillus niger that is precise, efficient and selectable [J ] . PLoS One , 2019 , 14 ( 1 ): e0210243 .

TONG S , AN K X , CHEN W X , et al . Evasion of Cas9 toxicity to develop an efficient genome editing system and its application to increase ethanol yield in Fusarium venenatum TB01 [J ] . Applied Microbiology and Biotechnology , 2022 , 106 ( 19 ): 6583 - 6593 .

GUSTAFSSON O , RÄDLER J , ROUDI S , et al . Efficient peptide-mediated in vitro delivery of Cas9 RNP [J ] . Pharmaceutics , 2021 , 13 ( 6 ): 878 .

DE VRIES R P , RILEY R , WIEBENGA A , et al . Comparative genomics reveals high biological diversity and specific adaptations in the industrially and medically important fungal genus Aspergillus [J ] . Genome Biology , 2017 , 18 ( 1 ): 28 .

POHL C , KIEL J W , DRIESSEN A M , et al . CRISPR/Cas9 based genome editing of Penicillium chrysogenum [J ] . ACS Synthetic Biology , 2016 , 5 ( 7 ): 754 - 764 .

SONG R J , ZHAI Q , SUN L , et al . CRISPR/Cas9 genome editing technology in filamentous fungi: progress and perspective [J ] . Applied Microbiology and Biotechnology , 2019 , 103 ( 17 ): 6919 - 6932 .

LIU Q , GAO R R , LI J G , et al . Development of a genome-editing CRISPR/Cas9 system in thermophilic fungal Myceliophthora species and its application to hyper-cellulase production strain engineering [J ] . Biotechnology for Biofuels , 2017 , 10 : 1 .

VAN DEN BERG M A , ALBANG R , ALBERMANN K , et al . Genome sequencing and analysis of the filamentous fungus Penicillium chrysogenum [J ] . Nature Biotechnology , 2008 , 26 ( 10 ): 1161 - 1168 .

SONG L T , OUEDRAOGO J P , KOLBUSZ M , et al . Efficient genome editing using tRNA promoter-driven CRISPR/Cas9 gRNA in Aspergillus niger [J ] . PLoS One , 2018 , 13 ( 8 ): e0202868 .

ZHENG X M , ZHENG P , ZHANG K , et al . 5S rRNA promoter for guide RNA expression enabled highly efficient CRISPR/Cas9 genome editing in Aspergillus niger [J ] . ACS Synthetic Biology , 2019 , 8 ( 7 ): 1568 - 1574 .

FULLER K K , CHEN S , LOROS J J , et al . Development of the CRISPR/Cas9 system for targeted gene disruption in Aspergillus fumigatus [J ] . Eukaryotic Cell , 2015 , 14 ( 11 ): 1073 - 1080 .

WEBER J , VALIANTE V , NØDVIG C S , et al . Functional reconstitution of a fungal natural product gene cluster by advanced genome editing [J ] . ACS Synthetic Biology , 2017 , 6 ( 1 ): 62 - 68 .

ZHANG P , ZHOU S , WANG G , et al . Two transcription factors cooperatively regulate DHN melanin biosynthesis and development in Pestalotiopsis fici [J ] . Molecular Microbiology , 2019 , 112 ( 2 ): 649 - 666 .

XIANG B Y , HAO X R , XIE Q H , et al . Deletion of a rare fungal PKS CgPKS11 promotes chaetoglobosin A bi osynthesis, yet defers the growth and development of Chaetomium globosum [J ] . Journal of Fungi , 2021 , 7 ( 9 ): 750 .

ALEKSENKO A , CLUTTERBUCK A J . Autonomous plasmid replication in Aspergillus nidulans : AMA1 and MATE elements [J ] . Fungal Genetics and Biology , 1997 , 21 ( 3 ): 373 - 387 .

KATAYAMA T , NAKAMURA H , ZHANG Y , et al . Forced recycling of an AMA1-based genome-editing plasmid allows for efficient multiple gene deletion/integration in the industrial filamentous fungus Aspergillus oryzae [J ] . Applied and Environmental Microbiology , 2019 , 85 ( 3 ): e01896 - e01818 .

NIELSEN M L , ISBRANDT T , RASMUSSEN K B , et al . Genes linked to production of secondary metabolites in Talaromyces atroroseus revealed using CRISPR-Cas9 [J ] . PLoS One , 2017 , 12 ( 1 ): e0169712 .

WENDEROTH M , PINECKER C , VOß B , et al . Establishment of CRISPR/Cas9 in Alternaria alternata [J ] . Fungal Genetics and Biology , 2017 , 101 : 55 - 60 .

SEEKLES S J , TEUNISSE P P P , PUNT M , et al . Preservation stress resistance of melanin deficient conidia from Paecilomyces variotii and Penicillium roqueforti mutants generated via CRISPR/Cas9 genome editing [J ] . Fungal Biology and Biotechnology , 2021 , 8 ( 1 ): 4 .

VAKULSKAS C A , BEHLKE M A . Evaluation and reduction of CRISPR off-target cleavage events [J ] . Nucleic Acid Therapeutics , 2019 , 29 ( 4 ): 167 - 174 .

ZOU G , XIAO M L , CHAI S X , et al . Efficient genome editing in filamentous fungi via an improved CRISPR-Cas9 ribonucleoprotein method facilitated by chemical reagents [J ] . Microbial Biotechnology , 2021 , 14 ( 6 ): 2343 - 2355 .

GRIJSEELS S , POHL C , NIELSEN J C , et al . Identification of the decumbenone biosynthetic gene cluster in Penicillium decumbens and the importance for production of calbistrin [J ] . Fungal Biology and Biotechnology , 2018 , 5 : 18 .

VALENTE S , PIOMBO E , SCHROECKH V , et al . CRISPR-Cas9-based discovery of the verrucosidin biosynthesis gene cluster in Penicillium polonicum [J ] . Frontiers in Microbiology , 2021 , 12 : 660871 .

FERRARA M , HAIDUKOWSKI M , LOGRIECO A F , et al . A CRISPR-Cas9 system for genome editing of Fusarium proliferatum [J ] . Scientific Reports , 2019 , 9 ( 1 ): 19836 .

MCLEAN K J , HANS M , MEIJRINK B , et al . Single-step fermentative production of the cholesterol-lowering drug pravastatin via reprogramming of Penicillium chrysogenum [J ] . Proceedings of the National Academy of Sciences of the United States of America , 2015 , 112 ( 9 ): 2847 - 2852 .

LIANG B , DU X J , LI P , et al . Investigation of citrinin and pigment biosynthesis mechanisms in Monascus purpureus by transcriptomic analysis [J ] . Frontiers in Microbiology , 2018 , 9 : 1374 .

LIU W W , AN C Y , SHU X , et al . A dual-plasmid CRISPR/cas system for mycotoxin elimination in polykaryotic industrial fungi [J ] . ACS Synthetic Biology , 2020 , 9 ( 8 ): 2087 - 2095 .

SILBERSTEIN S D , SHREWSBURY S B , HOEKMAN J . Dihydroergotamine (DHE) - then and now: a narrative review [J ] . Headache , 2020 , 60 ( 1 ): 40 - 57 .

YU L , XIAO M L , ZHU Z H , et al . Efficient genome editing in Claviceps purpurea using a CRISPR/Cas9 ribonucleoprotein method [J ] . Synthetic and Systems Biotechnology , 2022 , 7 ( 2 ): 664 - 670 .

CELIA-SANCHEZ B N , MANGUM B , BREWER M , et al . Analysis of Cyp51 protein sequences shows 4 major Cyp51 gene family groups across fungi [J ] . G3 Genes|Genomes|Genetics , 2022 , 12 ( 11 ): jkac249 .

GUO Z Q , LIU X Y , WANG N , et al . Membrane component ergosterol builds a platform for promoting effector secretion and virulence in Magnaporthe oryzae [J ] . The New Phytologist , 2023 , 237 ( 3 ): 930 - 943 .

ASSRESS H A , SELVARAJAN R , NYONI H , et al . Antifungal azoles and azole resistance in the environment: current status and future perspectives—a review [J ] . Reviews in Environmental Science and Bio/Technology , 2021 , 20 ( 4 ): 1011 - 1041 .

KHAN A A , FAROOQ F , JAIN S K , et al . Comparative host-pathogen interaction analyses of SARS-CoV2 and Aspergillus fumigatus , and pathogenesis of COVID-19-associated aspergillosis [J ] . Microbial Ecology , 2022 , 84 ( 4 ): 1236 - 1244 .

PÉREZ-CANTERO A , MARTIN-VICENTE A , GUARRO J , et al . Analysis of the cyp51 genes contribution to azole resistance in Aspergillus section Nigri with the CRISPR-Cas9 technique [J ] . Antimicrobial Agents and Chemotherapy , 2021 , 65 ( 5 ): e01996-20 .

ZHANG L H , ZHENG X M , CAIRNS T C , et al . Disruption or reduced expression of the orotidine-5′-decarboxylase gene pyrG increases citric acid production: a new discovery during recyclable genome editing in Aspergillus niger [J ] . Microbial Cell Factories , 2020 , 19 ( 1 ): 76 .

YANG L , HENRIKSEN M M , HANSEN R S , et al . Metabolic engineering of Aspergillus niger via ribonucleoprotein-based CRISPR-Cas9 system for succinic acid production from renewable biomass [J ] . Biotechnology for Biofuels , 2020 , 13 ( 1 ): 206 .

BRITO A F , MOREIRA L K S , MENEGATTI R , et al . Piperazine derivatives with central pharmacological activity used as therapeutic tools [J ] . Fundamental & Clinical Pharmacology , 2019 , 33 ( 1 ): 13 - 24 .

YUAN B C , LIU D , GUAN X , et al . Piperazine ring formation by a single-module NRPS and cleavage by an α-KG-dependent nonheme iron dioxygenase in brasiliamide biosynthesis [J ] . Applied Microbiology and Biotechnology , 2020 , 104 ( 14 ): 6149 - 6159 .

SZYMAŃSKI M , CHMIELEWSKA S , CZYŻEWSKA U , et al . Echinocandins-structure, mechanism of action and use in antifungal therapy [J ] . Journal of Enzyme Inhibition and Medicinal Chemistry , 2022 , 37 ( 1 ): 876 - 894 .

ZHANG F , LIU H , ZHANG T , et al . Biochemical and genetic characterization of fungal proline hydroxylase in echinocandin biosynthesis [J ] . Applied Microbiology and Biotechnology , 2018 , 102 ( 18 ): 7877 - 7890 .

WEI T Y , WU Y J , XIE Q P , et al . CRISPR/Cas9-based genome editing in the filamentous fungus Glarea lozoyensis and its application in manipulating gloF [J ] . ACS Synthetic Biology , 2020 , 9 ( 8 ): 1968 - 1977 .

WOODCRAFT C , CHOOI Y H , ROUX I . The expanding CRISPR toolbox for natural product discovery and engineering in filamentous fungi [J ] . Natural Product Reports , 2023 , 40 ( 1 ): 158 - 173 .

OMANAKUTTAN V K , JOHN J , HOPF H . Synthesis of 3(2 H )-furanones: a review [J ] . European Journal of Organic Chemistry , 2021 , 2021( 2 ): 163 - 201 .

WEI X X , MATSUYAMA T , SATO H , et al . Molecular and computational bases for spirofuranone formation in setosusin biosynthesis [J ] . Journal of the American Chemical Society , 2021 , 143 ( 42 ): 17708 - 17715 .

NØDVIG C S , HOOF J B , KOGLE M E , et al . Efficient oligo nucleotide mediated CRISPR-Cas9 gene editing in Aspergilli [J ] . Fungal Genetics and Biology , 2018 , 115 : 78 - 89 .

KUIVANEN J , WANG Y M J , RICHARD P . Engineering Aspergillus niger for galactaric acid production: elimination of galactaric acid catabolism by using RNA sequencing and CRISPR/Cas9 [J ] . Microbial Cell Factories , 2016 , 15 ( 1 ): 210 .

DONG L B , LIN X T , YU D , et al . High-level expression of highly active and thermostable trehalase from Myceliophthora thermophila in Aspergillus niger by using the CRISPR/Cas9 tool and its application in ethanol fermentation [J ] . Journal of Industrial Microbiology & Biotechnology , 2020 , 47 ( 1 ): 133 - 144 .

WONG G , LIM L R , TAN Y Q , et al . Reconstituting the complete biosynthesis of D-lysergic acid in yeast [J ] . Nature Communications , 2022 , 13 ( 1 ): 712 .

JASTRZĘBSKI M K , KACZOR A A , WRÓBEL T M . Methods of lysergic acid synthesis-the key ergot alkaloid [J ] . Molecules , 2022 , 27 ( 21 ): 7322 .

LEADMON C E , SAMPSON J K , MAUST M D , et al . Several Metarhizium species produce ergot alkaloids in a condition-specific manner [J ] . Applied and Environmental Microbiology , 2020 , 86 ( 14 ): e00373 - e00320 .

DAVIS K A , SAMPSON J K , PANACCIONE D G . Genetic reprogramming of the ergot alkaloid pathway of Metarhizium brunneum [J ] . Applied and Environmental Microbiology , 2020 , 86 ( 19 ): e01251 - e01220 .

WU C , CHEN Y M , QIU Y F , et al . A simple approach to mediate genome editing in the filamentous fungus Trichoderma reesei by CRISPR/Cas9-coupled in vivo gRNA transcription [J ] . Biotechnology Letters , 2020 , 42 ( 7 ): 1203 - 1210 .

WANG Q , ZHAO Q Q , LIU Q , et al . CRISPR/Cas9-mediated genome editing in Penicillium oxalicum and Trichoderma reesei using 5S rRNA promoter-driven guide RNAs [J ] . Biotechnology Letters , 2021 , 43 ( 2 ): 495 - 502 .

KATAYAMA T , TANAKA Y , OKABE T , et al . Development of a genome editing technique using the CRISPR/Cas9 system in the industrial filamentous fungus Aspergillus oryzae [J ] . Biotechnology Letters , 2016 , 38 ( 4 ): 637 - 642 .

ZHANG C , MENG X H , WEI X L , et al . Highly efficient CRISPR mutagenesis by microhomology-mediated end joining in Aspergillus fumigatus [J ] . Fungal Genetics and Biology , 2016 , 86 : 47 - 57 .

LIU Q , ZHANG Y L , LI F Y , et al . Upgrading of efficient and scalable CRISPR-Cas-mediated technology for genetic engineering in thermophilic fungus Myceliophthora thermophila [J ] . Biotechnology for Biofuels , 2019 , 12 : 293 .

HUANG J , ROWE D , SUBEDI P , et al . CRISPR-Cas12a induced DNA double-strand breaks are repaired by multiple pathways with different mutation profiles in Magnaporthe oryzae [J ] . Nature Communications , 2022 , 13 : 7168 .

JIA Y , XU R G , REN X J , et al . Next-generation CRISPR/Cas9 transcriptional activation in Drosophila using flySAM [J ] . Proceedings of the National Academy of Sciences of the United States of America , 2018 , 115 ( 18 ): 4719 - 4724 .

JANG S , JANG S , JUNG G Y . Toward tunable dynamic repression using CRISPRi [J ] . Biotechnology Journal , 2018 , 13 ( 9 ): e1800152 .

ROUX I , WOODCRAFT C , HU J Y , et al . CRISPR-mediated activation of biosynthetic gene clusters for bioactive molecule discovery in filamentous fungi [J ] . ACS Synthetic Biology , 2020 , 9 ( 7 ): 1843 - 1854 .

LI X J , HUANG L G , PAN L J , et al . CRISPR/dCas9-mediated epigenetic modification reveals differential regulation of histone acetylation on Aspergillus niger secondary metabolite [J ] . Microbiological Research , 2021 , 245 : 126694 .

KEYS H R , KNOUSE K A . Genome-scale CRISPR screening in a single mouse liver [J ] . Cell Genomics , 2022 , 2 ( 12 ): 100217 .

LI Z M , KIM K S . RELATe enables genome-scale engineering in fungal genomics [J ] . Science Advances , 2020 , 6 ( 38 ): eabb8783 .

LUU X C , SHIDA Y , SUZUKI Y , et al . A novel high-throughput approach for transforming filamentous fungi employing a droplet-based microfluidic platform [J ] . New Biotechnology , 2022 , 72 : 149 - 158 .

HEIGWER F , BOUTROS M . Cloud-based design of short guide RNA (sgRNA) libraries for CRISPR experiments [M ] //CRISPR Guide RNA Design, Methods in Molecular Biology. New York, NY : Springer US , 2020 : 3 - 22 .

LABUN K , MONTAGUE T G , GAGNON J A , et al . CHOPCHOPv2: a web tool for the next generation of CRASPR genome engineering . Nucleic Acid Res , 2016 , 44 ( W1 ): W272 - 276 .

HWANG G H , KIM J S , BAE S S . Web-based CRISPR toolkits: Cas-OFFinder, Cas-designer, and Cas-analyzer [M ] // CRISPR Gui de RNA Design, Methods in Molecular Biology. New York, NY : Springer US , 2020 : 23 - 33 .

LEISEN T , BIETZ F , WERNER J , et al . CRISPR/Cas with ribonucleoprotein complexes and transiently selected telomere vectors allows highly efficient marker-free and multiple genome editing in Botrytis cinerea [J ] . PLoS Pathogens , 2020 , 16 ( 8 ): e1008326 .

ABDULRACHMAN D , EURWILAICHITR L , CHAMPREDA V , et al . Development of a CRISPR/Cpf1 system for targeted gene disruption in Aspergillus aculeatus TBRC 277 [J ] . BMC Biotechnology , 2021 , 21 ( 1 ): 15 .

KIM B , KIM H J , LEE S J . Effective blocking of microbial transcriptional initiation by dCas9-NG-mediated CRISPR interference [J ] . Journal of Microbiology and Biotechnology , 2020 , 30 ( 12 ): 1919 - 1926 .

HAN H A , PANG J K S , SOH B S . Mitigating off-target effects in CRISPR/Cas9-mediated in vivo gene editing [J ] . Journal of Molecular Medicine , 2020 , 98 ( 5 ): 615 - 632 .

NGUYEN L T , MACALUSO N C , PIZZANO B L M , et al . A thermostable Cas12b from Brevibacillus leverages one-pot discrimination of SARS-CoV-2 variants of concern [J ] . eBioMedicine , 2022 , 77 : 103926 .

GHOUNEIMY A , MAHFOUZ M . Streamlined detection of SARS-CoV-2 via Cas13 [J ] . Nature Biomedical Engineering , 2022 , 6 ( 8 ): 925 - 927 .

ZHOU B , YANG R L , SOHAIL M , et al . CRISPR/Cas14 provides a promising platform in facile and versatile aptasensing with improved sensitivity [J ] . Talanta , 2023 , 254 : 124120 .

HAN X , LIU Z B , JO M C , et al . CRISPR-Cas9 delivery to hard-to-transfect cells via membrane deformation [J ] . Science Advances , 2015 , 1 ( 7 ): e1500454 .

AMALAMOL D , ASHWIN N M R , LAKSHANA K V , et al . A highly efficient stratagem for protoplast isolation and genetic transformation in filamentous fungus Colletotrichum falcatum [J ] . Folia Microbiologica , 2022 , 67 ( 3 ): 479 - 490 .

WANG J , TENG Y X , GONG X Y , et al . Exploring and engineering PAM-diverse Streptococci Cas9 for PAM-directed bifunctional and titratable gene control in bacteria [J ] . Metabolic Engineering , 2023 , 75 : 68 - 77 .

FU Y W , DAI X Y , WANG W T , et al . Dynamics and competition of CRISPR-Cas9 ribonucleoproteins and AAV donor-mediated NHEJ, MMEJ and HDR editing [J ] . Nucleic Acids Research , 2021 , 49 ( 2 ): 969 - 985 .

ENDO M , IWAKAMI S , TOKI S . Precision genome editing in plants via gene targeting and subsequent break-induced single-strand annealing [J ] . Plant Biotechnology Journal , 2021 , 19 ( 3 ): 563 - 574 .

NEUGEBAUER M E , HSU A , ARBAB M , et al . Evolution of an adenine base editor into a small, efficient cytosine base editor with low off-target activity [J/OL ] . Nature Biotechnology , 2022 [ 2023-01-03 ] . https://www.nature.com/articles/s41587-022-01533-6 https://www.nature.com/articles/s41587-022-01533-6 .

HUANG L G , DONG H Z , ZHENG J W , et al . Highly efficient single base editing in Aspergillus niger with CRISPR/Cas9 cytidine deaminase fusion [J ] . Microbiological Research , 2019 , 223/ 224 / 225 : 44 - 50 .

ZHANG C Y , LI N , RAO L , et al . Development of an efficient C-to-T base-editing system and its application to cellulase transcription factor precise engineering in thermophilic fungus Myceliophthora thermophila [J ] . Microbiology Spectrum , 2022 , 10 ( 3 ): e0232121 .

JIN S , ZONG Y , GAO Q , et al . Cytosine, but not adenine, base editors induce genome-wide off-target mutations in rice [J ] . Science , 2019 , 364 ( 6437 ): 292 - 295 .

刘倩 , 李金根 , 张晨阳 , 等 . 工业丝状真菌基因组编辑技术研究进展 [J ] . 合成生物学 , 2021 , 2 ( 2 ): 256 - 273 .

LIU Q , LI J G , ZHANG C Y , et al . Research progress of genome editing technologies for industrial filamentous fungi [J ] . Synthetic Biology Journal , 2021 , 2 ( 2 ): 256 - 273 .

肖晗 , 刘宜欣 . CRISPR-Cas系统编辑丝状真菌的进展与挑战 [J ] . 合成生物学 , 2021 , 2 ( 2 ): 274 - 286 .

XIAO H , LIU Y X . Progress and challenge of the CRISPR-Cas system in gene editing for filamentous fungi [J ] . Synthetic Biology Journal , 2021 , 2 ( 2 ): 274 - 286 .