希瓦氏菌
Shewanella sp. MR-4在不同末端电子受体条件下的代谢通量分析Metabolic flux analysis of
Shewanella sp. MR-4 with different terminal electron receptors- 合成生物学 2023年4卷第6期 页码：1300-1320
- 作者机构：
漳州人才发展集团有限公司，福建 漳州 363000
- 作者简介：
[ "郑庆祥（1983—），男，博士，工程师，漳州人才发展集团有限公司副总经理。研究方向为微生物代谢工程。E-mail：179949046@qq.com" ]
- 基金信息：国家自然科学基金(30970035)
DOI：10.12211/2096-8280.2022-052
中图分类号： Q816收稿：2022-09-23，
修回：2022-11-16，
纸质出版：2023-12-31
- 稿件说明：
移动端阅览
郑庆祥. 希瓦氏菌Shewanella sp. MR-4在不同末端电子受体条件下的代谢通量分析［J］. 合成生物学， 2023， 4（6）： 1300-1320
ZHENG Qingxiang. Metabolic flux analysis of Shewanella sp. MR-4 with different terminal electron receptors［J］. Synthetic Biology Journal， 2023， 4（6）： 1300-1320
郑庆祥. 希瓦氏菌Shewanella sp. MR-4在不同末端电子受体条件下的代谢通量分析［J］. 合成生物学， 2023， 4（6）： 1300-1320 DOI： 10.12211/2096-8280.2022-052.
ZHENG Qingxiang. Metabolic flux analysis of Shewanella sp. MR-4 with different terminal electron receptors［J］. Synthetic Biology Journal， 2023， 4（6）： 1300-1320 DOI： 10.12211/2096-8280.2022-052.
摘要
本研究解析了
Shewanella
sp. MR-4在厌氧条件下以Fe
3+
、延胡索酸、硝酸和二甲基亚砜（DMSO）为末端电子受体时代谢通量分布，发现在各种不同末端电子受体条件下细胞内代谢通量分布发生显著改变。与延胡索酸相比，在Fe
3+
和硝酸为电子受体条件下，乙酸生成速率明显降低，而三羧酸循环代谢通量增加。同时，三羧酸循环回补反应存在显著差异：在延胡索酸为电子受体条件下，磷酸烯醇式丙酮酸羧化酶反应是唯一回补反应，而在Fe
3+
和硝酸条件下苹果酸酶催化成为唯一回补反应。本研究在代谢通量分析基础上定量解析了还原力NADH合成和消耗，分析了电子流向变化，发现在不同末端电子受体条件下，NADH产生途径和反应发生明显变化。研究结果为希瓦氏菌代谢工程改造提供了一定理论依据。
Abstract
Shewanella
is ubiquitous in natural environments
known to show various unique activities
such as metal reduction and trimethylamine production. The metal reduction capability expands the range of electron acceptors and enables its application in producing current in microbial fuel cells. Previous work primarily focused on the model organism
Shewanella oneidensis
MR-1
based on which the metabolic models were constructed for three other strains including
Shewanella
sp. MR-4. Interestingly
the genetic similarity does not always coincide with metabolic phenotype similarity between these two strains
suggesting that potential regulatory effects affecting cellular phenotypes are present in
Shewanella
sp.
MR-4. This study analyzed the metabolic flux distribution of
Shewanella
sp. MR-4 when Fe
3+
fumaric acid
nitric acid and dimethyl sulfoxide were used as electron acceptors under anaerobic conditions. The intracellular metabolic flux distribution was significantly changed with different electron acceptors. The formation rate of acetic acid was decreased
while the metabolic flux of the tricarboxylic acid (TC
A) cycle was increased with Fe
3+
and nitric acid as electron acceptors
compared to that with fumaric acid. The anaplerotic reaction in the TCA cycle was also dramatically altered: the phosphoenolpyruvate carboxylase-catalyzed reaction or malic enzyme-catalyzed reaction is the only anaplerotic reaction with fumaric acid or Fe
3+
and nitric acid as electron acceptor
respectively. Based on the metabolic flux map
quantitative analysis was performed to derive the synthesis and consumption rates of reducing agent NADH
and the change of electron transfer. It was found that NADH metabolism varies significantly with the electron acceptors tested. The results provide a theoretical basis for the future metabolic engineering of
Shewanella
and experimental evidences that can be used for future study of different phenotypes unaccounted for in previous models of
Shewanella
sp. MR-4 and MR-1.
2
关键词
Keywords
references
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