调控工程在光合蓝细菌中的应用

董正鑫; 孙韬; 陈磊; 张卫文

doi:10.12211/2096-8280.2022-012

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调控工程在光合蓝细菌中的应用

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- 调控工程在光合蓝细菌中的应用
- Applications of regulatory engineering in photosynthetic cyanobacteria
- 合成生物学 2022年3卷第5期页码：966-984
- 作者机构：
  
  1.天津大学化工学院合成微生物学实验室，天津 300072
  2.天津大学生物安全战略研究中心，天津 300072
  3.教育部系统生物工程重点实验室，天津 300072
  4.教育部合成生物学前沿科学中心，天津 300072
  5.化学化工协同创新中心，天津 300072
- 作者简介：
  
  [ "董正鑫（1997—），男，博士研究生。主要研究方向为微生物合成生物学。 E-mail：dongxin7122@foxmail.com" ]
  [ "孙韬（1990—），博士，讲师，硕士生导师。从事微生物合成生物学及生物安全相关研究。 E-mail：tsun@tju.edu.cn" ]
  [ "张卫文（1967—），男，博士，教授，博士生导师，生物安全战略研究中心主任。长期从事光合微生物的合成生物学，以及生物安全相关研究，牵头中美合成生物学生物安全“二轨”战略对话。 E-mail：wwzhang8@tju.edu.cn" ]
- 基金信息：
  
  国家重点研发计划(2018YFA0903000;2019YFA0904600;2018YFA0903600)
- DOI：10.12211/2096-8280.2022-012
  中图分类号： Q819
- 收稿：2022-02-11，
  
  修回：2022-04-01，
  
  纸质出版：2022-10-31
- 稿件说明：
移动端阅览
董正鑫，孙韬，陈磊，张卫文. 调控工程在光合蓝细菌中的应用［J］. 合成生物学， 2022， 3（5）： 966-984

DONG Zhengxin， SUN Tao， CHEN Lei， ZHANG Weiwen. Applications of regulatory engineering in photosynthetic cyanobacteria［J］. Synthetic Biology Journal， 2022， 3（5）： 966-984
董正鑫，孙韬，陈磊，张卫文. 调控工程在光合蓝细菌中的应用［J］. 合成生物学， 2022， 3（5）： 966-984 DOI： 10.12211/2096-8280.2022-012.

DONG Zhengxin， SUN Tao， CHEN Lei， ZHANG Weiwen. Applications of regulatory engineering in photosynthetic cyanobacteria［J］. Synthetic Biology Journal， 2022， 3（5）： 966-984 DOI： 10.12211/2096-8280.2022-012.

摘要

能源短缺与环境污染问题限制着人类发展，光合蓝细菌因能够利用太阳能将CO

固定生成燃料和化学品而受到广泛关注。迄今为止，在光合蓝细菌中已实现近百种燃料和化学品由CO

的生物合成，有望促进CO

的资源化利用并助力“碳中和”。调控工程能够实现基因表达多层次调控及代谢网络的全局性调控，是提高光合蓝细菌CO

固定效率的有效手段。本文首先归纳了光合蓝细菌底盘中的双组分信号转导系统、调控小RNA和σ因子等3种主要调控系统的分类、作用过程以及功能；介绍了光合蓝细菌调控系统中的调控元件功能研究，系统总结了光合蓝细菌中通过调控系统元件改造，提高底盘鲁棒性、优化产品生产所进行的调控工程；最后，讨论了光合蓝细菌中调控工程的未来研究方向，重点包括调控系统功能阐明、工具开发、多基因调控、调控系统蛋白工程改造和系统调控工程等。总之，有望通过系统调控工程，实现对光合蓝细菌底盘细胞全局代谢网络的精确调控。

Abstract

Energy shortage and environmental pollution are the limiting factors for the development of human society. Photosynthetic cyanobacteria have attracted increasing attention due to their ability to use solar energy for the fixation of CO

for green production of biofuels and chemicals under low nutrient conditions. So far

nearly 100 kinds of fuels and chemicals have been synthesized in photosynthetic cyanobacteria directly from CO

which is expected to promote CO

utilization and contribute to “carbon neutrality”. However

the industrial applications of photosynthetic cyanobacteria are restricted by factors such a

s their slow growth rate

low biomass

low product yield

and poor environmental robustness. Regulatory engineering

enabling global regulation of metabolic networks and multi-level regulation of genes expression

is a powerful tool to address these challenges. In this paper

we firstly introduce the classification

mechanism

and function of three major regulatory systems

including two-component signal transduction systems (TCS)

regulatory small RNAs (sRNAs)

and σ factor in photosynthetic cyanobacteria. Subsequently

we briefly discuss the functions of regulatory elements in the regulatory systems of photosynthetic cyanobacteria related to the tolerance against high salt

short-chain alcohols

light stress

metal ions

oxidative stress

as well as heat and drought

and the regulation of carbon metabolism in the production of the target components. We systematically review the applications of regulatory engineering

based on engineered regulatory system elements

in improving the robustness of photosynthetic cyanobacteria under the above stress conditions and in optimizing the carbon fluxes towards product biosynthesis. Finally

we conclude with the future research focuses of regulatory engineering in photosynthetic cyanobacteria

highlighting research areas such as functional elucidation of the regulatory systems

toolbox development

multi-gene regulations

protein engineering of the regulatory systems

and systematic regulatory engineering. In a word

the regulatory systems are expected to be artificially designed through regulatory engineering to achieve accurate control of the global metabolic network to improve the robustness and cell growth of photosynthetic cyanobacteria

and more importantly the titer

yield

and productivity of the target components for industry-scale applications.

关键词

Keywords

references

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