工程酵母助力真菌嵌合萜类合酶的快速系统挖掘

范震; 潘海学; 唐功利

doi:10.12211/2096-8280.2021-084

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工程酵母助力真菌嵌合萜类合酶的快速系统挖掘

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- 工程酵母助力真菌嵌合萜类合酶的快速系统挖掘
- Engineered yeast facilitates rapid and systematic mining of fungal chimeric terpene synthases
- 合成生物学 2021年2卷第5期页码：666-673
- 作者机构：
  
  1.中国科学院大学杭州高等研究院，化学与材料科学学院，浙江杭州 310024
  2.中国科学院上海有机化学研究所，生命有机化学国家重点实验室，上海 200032
- 作者简介：
  
  范震（1987—），男，博士，博士后，主要从事天然产物生物合成研究。
  唐功利（1971—），男，博士，研究员，主要从事天然产物生物合成和化学生物学研究。
- 基金信息：
  
  国家自然科学基金重点项目(31930002)
- DOI：10.12211/2096-8280.2021-084
  中图分类号： Q81
- 收稿：2021-08-13，
  
  修回：2021-08-29，
  
  纸质出版：2021-10-31
- 稿件说明：
移动端阅览
范震，潘海学，唐功利. 工程酵母助力真菌嵌合萜类合酶的快速系统挖掘［J］. 合成生物学， 2021， 2（5）： 666-673

FAN Zhen， PAN Haixue， TANG Gongli. Engineered yeast facilitates rapid and systematic mining of fungal chimeric terpene synthases［J］. Synthetic Biology Journal， 2021， 2（5）： 666-673
范震，潘海学，唐功利. 工程酵母助力真菌嵌合萜类合酶的快速系统挖掘［J］. 合成生物学， 2021， 2（5）： 666-673 DOI： 10.12211/2096-8280.2021-084.

FAN Zhen， PAN Haixue， TANG Gongli. Engineered yeast facilitates rapid and systematic mining of fungal chimeric terpene synthases［J］. Synthetic Biology Journal， 2021， 2（5）： 666-673 DOI： 10.12211/2096-8280.2021-084.

摘要

真菌特有的嵌合萜类合酶（简称为PTTS）由C端异戊烯基转移酶（PT）结构域和N端I型萜类合酶（TS）结构域组成，可直接催化异戊二烯单元产生结构多样的二萜和二倍半萜化合物。自2007年发现第一个PTTS以来，只有约20个PTTS的功能被验证，人们对PTTS的起源和功能进化的认识还十分有限。最近武汉大学刘天罡教授课题组与美国田纳西大学陈峰教授课题组合作，运用高效萜类前体供给的酿酒酵母底盘并结合高通量的自动化平台，对PTTS的起源和功能进化进行了深入系统的探索，扩充了嵌合萜类合酶家族成员的数量，并为萜类化合物的挖掘提供了一个高效的方法。

Abstract

Chimeric terpene synthases (PTTSs)

which consist of C-terminal prenyltransferase (PT) and N-terminal ClassⅠterpene synthase (TS) domains

are unique to fungi for catalyzing the synthesis of structurally diverse diterpenes and sesterterpenes. Since the first PTTS was discovered in 2007

only about 20 PTTSs have been functionally verified

and understanding of the origin and functional evolution of PTTS genes is very limited. Recently

Professor Tiangang Liu

s research team at Wuhan University and Professor Feng Chen

s research team at the University of Tennessee in the United States have conducted in-depth exploration on the origin and functional evolution of PTTS with an efficient terpene precursor yeast chassis system combined with the high-throughput automatic screening platform. The research not only expands the chimeric terpene synthase family

but also provides an advanced method for mining more terpene synthases for the biosynthesis of terpenoids.

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references

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