基于生物正交反应的病毒功能化及其生物医学应用

黄利利; 张韩; 王伟伟; 谢海燕

doi:10.12211/2096-8280.2021-055

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基于生物正交反应的病毒功能化及其生物医学应用

特约评述 | 更新时间：2025-03-20

- 基于生物正交反应的病毒功能化及其生物医学应用
- Bioorthogonal functionalization of viruses for biomedical applications
- 合成生物学 2022年3卷第2期页码：335-351
- 作者机构：
  
  1.北京理工大学医工融合研究院，北京 100081
  2.北京理工大学生命学院，北京 100081
- 作者简介：
  
  [ "黄利利（1984—），女，博士，特别研究员，博士生导师。研究方向：建立病毒的荧光标记新方法，并实时动态示踪其侵染过程，为诠释病毒的感染机制和相应药物的研发提供依据；构建具有肿瘤诊疗一体化功能的溶瘤病毒纳米颗粒，推动溶瘤病毒的临床应用。 E-mail：llhuang@bit.edu.cn" ]
  [ "谢海燕（1975—），女，博士，教授，博士生导师。研究方向：仿生生物探针构建及其活体成像应用；智能纳米生物医学诊疗系统构建与应用；溶瘤病毒工程化改造。 E-mail：hyanxie@bit.edu.cn" ]
- 基金信息：
  
  国家自然科学基金(21904012;21874011;91859123);国家杰出青年科学基金(22025401)
- DOI：10.12211/2096-8280.2021-055
  中图分类号： Q816
- 收稿：2021-05-03，
  
  修回：2021-08-27，
  
  纸质出版：2022-04-30
- 稿件说明：
移动端阅览
黄利利，张韩，王伟伟，谢海燕. 基于生物正交反应的病毒功能化及其生物医学应用［J］. 合成生物学， 2022， 3（2）： 335-351

HUANG Lili， ZHANG Han， WANG Weiwei， XIE Haiyan. Bioorthogonal functionalization of viruses for biomedical applications［J］. Synthetic Biology Journal， 2022， 3（2）： 335-351
黄利利，张韩，王伟伟，谢海燕. 基于生物正交反应的病毒功能化及其生物医学应用［J］. 合成生物学， 2022， 3（2）： 335-351 DOI： 10.12211/2096-8280.2021-055.

HUANG Lili， ZHANG Han， WANG Weiwei， XIE Haiyan. Bioorthogonal functionalization of viruses for biomedical applications［J］. Synthetic Biology Journal， 2022， 3（2）： 335-351 DOI： 10.12211/2096-8280.2021-055.

摘要

病毒具有分散性好、结构规则、可大量复制等特性，使其在生物医学领域的应用日益受到研究者关注。目前大多数基于病毒的生物医学应用主要需要将其与荧光探针、肿瘤识别分子等不同功能元件组装，进而赋予病毒可视化、免疫相容、靶向等性能。对于包膜病毒而言，其结构组成主要包括：包膜、衣壳和核酸。因此，组成病毒的生物大分子（蛋白质、糖类、脂类和核酸），均可作为靶标与不同元件进行可控组装和功能整合。近年来，基于生物正交反应的生物大分子修饰策略已经被广泛应用于病毒不同组分的工程化改造。本文概述了常用于生物大分子修饰的生物正交反应类型与特点，以及生物正交反应对病毒不同组分的改造策略；同时，介绍了病毒功能化在病毒动态示踪、疫苗开发、病毒检测、递送载体构建等领域的研究进展。生物正交反应技术的发展，将推动病毒功能化改造策略的进一步完善，进而拓展病毒的应用方向。

Abstract

The biomedical applications of viruses have attracted the attention of researchers because of their unique properties such as excellent dispersion

stable structure

and mass production. At present

most of viruses for such a purpose need to be assembled with different functional components including fluorescent probes

targeting ligands

therapeutic molecules

and so on

to endow them with required performance

for example

visualization

immune compatibility

specific targeting

and others. Before integrating the viruses with these functional components

they must be modified. The structure of enveloped viruses consists of nucleic acid

capsid

and envelope. Therefore

biological macromolecules such as proteins

polysaccharides

phospholipids

and nucleic acids can all be used as targets for the structural modification of the viruses. The viral protein component can be derivatized and functionalized genetically or post-translationally. For example

functional proteins or peptides can be genetically fused with the viral capsid directly. However

the other biomolecules including glycans

lipids

nucleic acids

and various metabolites are not amenable as such genetically encoded tags. Bioorthogonal reactions through which the compatible reactive groups can selectively conjugate with each other under physiological conditions

and also they are not toxic to cells and organisms. The major features of these reactions include: outstanding reliability

specific selectivity

and good compatibility with naturally occurring functional groups

making them a powerful tool for studying the structure and function of viruses.In this article

major characteristics of these bioorthogonal reactions are summarized. Subsequently

general schemes for bioorthogonal modifications on different viral components are depicted. Furthermore

we review the recent applications of bioorthogonal reactions in virus-related research

including viral tracking

vaccine development

the diagnosis of viral infections

and virus-based delivery systems. Finally

we conclude that based on the structure and application of viruses

researchers could select appropriate bioorthogonal reactions for viruses engineering

and other strategies

such as genetic engineering

biological coupling

and so on

could also be used to modify viruses to expand their applications.

关键词

Keywords

references

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