基于合成受体的食品污染物生物检测进展

胥欣欣; 匡华

doi:10.12211/2096-8280.2021-048

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基于合成受体的食品污染物生物检测进展

特约评述 | 更新时间：2025-03-20

- 基于合成受体的食品污染物生物检测进展
- Advances in the biological detection of food contaminants based on synthetic receptors
- 合成生物学 2022年3卷第2期页码：399-414
- 作者机构：
  
  1.江南大学食品科学技术国家重点实验室，江苏无锡 214122
  2.江南大学食品学院，生物界面与生物检测研究所，江苏无锡 214122
- 作者简介：
  
  [ "胥欣欣（1989—），男，博士，博士后研究员。研究方向为功能性生物大分子蛋白的规模化制备及应用。 E-mail：xuxinxin@jiangnan.edu.cn" ]
  [ "匡华（1981—），女，博士，教授，主要从事食品中危害物质快速检测和手性纳米生物探针构建等工作。研究方向包括单克隆抗体和重组受体开发、分子识别、信号放大与传感新策略。 E-mail：kuangh@jiangnan.edu.cn" ]
- 基金信息：
  
  国家重点研发计划(2018YFC1602303)
- DOI：10.12211/2096-8280.2021-048
  中图分类号： TS 207.3
- 收稿：2021-04-21，
  
  修回：2021-09-13，
  
  纸质出版：2022-04-30
- 稿件说明：
移动端阅览
胥欣欣，匡华. 基于合成受体的食品污染物生物检测进展［J］. 合成生物学， 2022， 3（2）： 399-414

XU Xinxin， KUANG Hua. Advances in the biological detection of food contaminants based on synthetic receptors［J］. Synthetic Biology Journal， 2022， 3（2）： 399-414
胥欣欣，匡华. 基于合成受体的食品污染物生物检测进展［J］. 合成生物学， 2022， 3（2）： 399-414 DOI： 10.12211/2096-8280.2021-048.

XU Xinxin， KUANG Hua. Advances in the biological detection of food contaminants based on synthetic receptors［J］. Synthetic Biology Journal， 2022， 3（2）： 399-414 DOI： 10.12211/2096-8280.2021-048.

摘要

在细胞生物学中，受体是指在细胞表面或细胞内任何能够与激素、药物、信号分子等配体结合，从而引起细胞功能变化的生物大分子。随着生物学的快速发展，各种天然的、非天然的化合物在细胞中的识别、转运等信号通路及分子作用机制已被逐渐解析。酶、离子通道、转运蛋白等生物靶标都可以归类为广义上的受体。类似于抗体-抗原，受体-配体反应同样具有高亲和力、高特异性和高饱和，在食品安全快速检测领域有一定的发展潜力。受体蛋白的定向进化设计、潜在受体的开发利用以及多学科技术的交叉互融是受体生物传感分析方法发展的巨大推动力。本文简单介绍了受体的分类以及受体-配体的关系，概述了合成生物学中不同底盘生物对受体蛋白量产化的偏好性，回顾了基于受体蛋白的筛查分析方法在食品安全检测相关领域的研究进展，如抗生素残留、农药残留、非法使用添加剂、生物毒素及生物性污染等。最后，探讨了受体结合测定法的优缺点，分析了目前基于受体的分析方法所面临的瓶颈问题和可能的解决方式，展望了合成受体在食品安全检测应用领域中的发展方向。

Abstract

In cell biology

receptors are biological macromolecules that can bind to hormones

drugs

signaling molecules

and other ligands inside cells or on their surfaces

thereby causing changes in the functions of these cells. With the rapid development of biology

signal pathways and molecular mechanisms

such as the recognition and transport of various natural and unnatural compounds in cells

have been gradually elucidated. Biological targets

for example

enzymes

ion channels and transporters

can be classified as receptors in a broad sense. Similar to antibody-antigen

the receptor-ligand binding also shows high affinity

specificity and saturation

leading to its potential applications in the fast detection for food safety. Here

we briefly introduce the classification of receptors and the relationship between receptors and ligands. The preparations of receptor proteins

including the selection of chassis organisms such as

E. coli

yeast

insect cells and mammalian cells for protein mass production

the utilization of cell-free translational systems

the process of protein expression and functional improvement

are also summarized. The main topic of this article is to review the research progress of receptor-based screening and analytical methods in fast detection for food safety

such as antibiotic residues

pesticide residues

illegal use of additives

biotoxins and biological pollutants. In addition

we also discuss

the advantages and disadvantages of receptor-binding assays compared to antibody-based analytic methods

and highlight the current bottlenecks for the receptor-binding assay and possible solutions. Finally

we prospect the development of synthetic receptors in the application for food safety assurance. The directed evolution of receptor proteins that is based on rational design

the further exploitation and wide utilization of potential receptors

and the combination of multidisciplinary technologies will provide a driving force for the development and promotion of the receptor-based analytical methods. Such methods have great potential

and will be used in research or for commercial purpose accordingly.

关键词

Keywords

references

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