基于合成生物技术构建高效生物制造系统的研究进展

张晓龙; 王晨芸; 刘延峰; 李江华; 刘龙; 堵国成

doi:10.12211/2096-8280.2021-015

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基于合成生物技术构建高效生物制造系统的研究进展

特约评述 | 更新时间：2025-03-20

- 基于合成生物技术构建高效生物制造系统的研究进展
- Research progress of constructing efficient biomanufacturing system based on synthetic biotechnology
- 合成生物学 2021年2卷第6期页码：863-875
- 作者机构：
  
  1.江南大学糖化学与生物技术教育部重点实验室，江苏无锡 214122
  2.江南大学未来食品科学中心，江苏无锡 214122
  3.江南大学工业生物技术教育部重点实验室，江苏无锡 214122
- 作者简介：
  
  [ "张晓龙（1988—），男，博士，助理研究员。研究方向为发酵工程。E-mail：qingshuang0302@163.com" ]
  [ "堵国成（1965—），男，博士，教授。研究方向为发酵工程与酶工程。E-mail：gcdu@jiangnan.edu.cn" ]
- 基金信息：
  
  国家重点研发计划(2018YFA0900300);国家自然科学基金(31972854)
- DOI：10.12211/2096-8280.2021-015
  中图分类号： Q815
- 收稿：2021-02-02，
  
  修回：2021-08-09，
  
  纸质出版：2021-12-31
- 稿件说明：
移动端阅览
张晓龙，王晨芸，刘延峰，李江华，刘龙，堵国成. 基于合成生物技术构建高效生物制造系统的研究进展［J］. 合成生物学， 2021， 2（6）： 863-875

ZHANG Xiaolong， WANG Chenyun， LIU Yanfeng， LI Jianghua， LIU Long， DU Guocheng. Research progress of constructing efficient biomanufacturing system based on synthetic biotechnology［J］. Synthetic Biology Journal， 2021， 2（6）： 863-875
张晓龙，王晨芸，刘延峰，李江华，刘龙，堵国成. 基于合成生物技术构建高效生物制造系统的研究进展［J］. 合成生物学， 2021， 2（6）： 863-875 DOI： 10.12211/2096-8280.2021-015.

ZHANG Xiaolong， WANG Chenyun， LIU Yanfeng， LI Jianghua， LIU Long， DU Guocheng. Research progress of constructing efficient biomanufacturing system based on synthetic biotechnology［J］. Synthetic Biology Journal， 2021， 2（6）： 863-875 DOI： 10.12211/2096-8280.2021-015.

摘要

基于合成生物技术构建绿色高效的生物制造系统是实现可持续化发展的重要途径，该技术的发展应用有望为食品、能源、医药、化工以及畜牧养殖等行业带来革命性的技术变革。本文针对基于合成生物技术构建高效生物制造系统进行系统性的总结与讨论。首先概述了代谢工程、酶工程、辅助系统优化以及发酵过程控制等技术的研究进展；其次，着重对比总结了大肠杆菌、芽孢杆菌属、谷氨棒酸杆菌以及酵母属等典型模式宿主的代谢特性，探究了各微生物制造系统的适用范围。最后，对合成生物技术在构建高效生物制造系统领域中的应用前景进行了展望。精细多元的代谢工程技术、高效简便的酶工程策略以及数字化的微生物系统将是促进高效生物制造系统构建的新引擎与新动力。

Abstract

Efficient and environmentally friendly biomanufacturing system based on synthetic biotechnology is an important approach to achieve sustainable development. Synthetic biology is expected to bring revolutionary technological breakthroughs in various industries

such as food

pharmacy and chemistry

as well as farming and animal husbandry. In this paper

the latest advances of technologies and strategies in synthetic biology used in the progress of constructing efficient biomanufacturing systems were introduced. Four aspects

namely metabolic regulation of key genes

enzyme engineering

cofactor engineering and fermentation optimization

were discussed. Through these technologies

engineered microorganisms with high robustness and excellent performance were constructed. Secondly

an emphasis was put on the summary of diverse metabolic characteristics of typical model organisms at present. In this part

as many as seven strains were mentioned

such as

Escherichia coli

Bacillus subtilis

Bacillus amyloliquefaciens

Bacillus licheniformis

Corynebacterium glutamicum

Pichia pastoris

and

Saccharomyces cerevisiae

. And for each strain

the advantages and disadvantages of different typical model organisms were discussed to clarify the scope of their most suitable products.

Escherichia coli

is the most intensively studied typical model organism system

making it the preferred expression system for proteins of interest. However

insufficient post-translational processing limits its applications for expressing eukaryotic-derived proteins.

Saccharomyces cerevisiae

and

Pichia pastoris

make up for this deficiency. Yeast expression system has significant advantages for the synthesis of natural products from plant

due to the extensive and in-depth research of P450 enzymes

such as the biosynthesis of artemisinin. Lastly

application prospects of synthetic biology in constructing efficient biomanufacturing systems were discussed. With the developments in standard synthetic biology components and data

standard automated work platforms

precise and generally applicable engineering strategies

emerging of machine learning and synthetic biology

it is expected to facilitate efficient biological manufacturing system construction. Precise and various metabolic engineering technology

flexible and convenient enzyme engineering strategies and whole cell microorganism modeling would be the new driving force for efficient biomanufacturing system construction.

关键词

Keywords

references

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