基于化学品生物合成的嗜甲烷菌人工细胞构建及应用进展

郭树奇; 焦子悦; 费强

doi:10.12211/2096-8280.2021-011

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基于化学品生物合成的嗜甲烷菌人工细胞构建及应用进展

特约评述 | 更新时间：2025-03-20

- 基于化学品生物合成的嗜甲烷菌人工细胞构建及应用进展
- Progress in construction and applications of methanotrophic cell factory for chemicals biosynthesis
- 合成生物学 2021年2卷第6期页码：1017-1029
- 作者机构：
  
  1.西安交通大学化学工程与技术学院，陕西西安 710049
  2.陕西省能源化工过程强化重点实验室，陕西西安 710049
- 作者简介：
  
  [ "郭树奇（1989—），男，博士、助理教授。研究方向为微生物代谢工程及合成生物学。E-mail：shuqguo@xjtu.edu.cn" ]
  [ "费强（1980—），男，教授，博士生导师。研究方向为围绕构建人工细胞将一碳气体高效转化为平台化学品和生物能源，并对其放大工艺进行技术经济可行性分析。E-mail：feiqiang@xjtu.edu.cn" ]
- 基金信息：
  
  国家重点研发计划(2018YFA0901500);国家自然科学基金(21878241);陕西省重点研发计划(2021SF-103)
- DOI：10.12211/2096-8280.2021-011
  中图分类号： Q819
- 收稿：2021-01-25，
  
  修回：2021-04-30，
  
  纸质出版：2021-12-31
- 稿件说明：
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郭树奇，焦子悦，费强. 基于化学品生物合成的嗜甲烷菌人工细胞构建及应用进展［J］. 合成生物学， 2021， 2（6）： 1017-1029

GUO Shuqi， JIAO Ziyue， FEI Qiang. Progress in construction and applications of methanotrophic cell factory for chemicals biosynthesis［J］. Synthetic Biology Journal， 2021， 2（6）： 1017-1029
郭树奇，焦子悦，费强. 基于化学品生物合成的嗜甲烷菌人工细胞构建及应用进展［J］. 合成生物学， 2021， 2（6）： 1017-1029 DOI： 10.12211/2096-8280.2021-011.

GUO Shuqi， JIAO Ziyue， FEI Qiang. Progress in construction and applications of methanotrophic cell factory for chemicals biosynthesis［J］. Synthetic Biology Journal， 2021， 2（6）： 1017-1029 DOI： 10.12211/2096-8280.2021-011.

摘要

由于来源广泛且储量丰富，甲烷被认为是极具应用潜力的下一代生物碳源。嗜甲烷菌是一种分离自富含甲烷环境中的革兰氏阴性细菌，其体内含有独特的甲烷单加氧酶能够让这类微生物以甲烷为唯一碳源和能源进行生长、代谢与产物合成。作为一种重要的工业微生物，嗜甲烷菌在甲烷生物转化利用、温室气体减排和“碳中和”策略开发方面具有重要意义。近年来，随着嗜甲烷菌基因编辑方法、代谢路径调控、生物元件挖掘等菌种构建工具和策略的不断开发，嗜甲烷菌人工细胞可高效转化甲烷生物合成多种大宗化学品和生物燃料。本文围绕遗传改造工具、甲烷碳流调控、异源途径表达和代谢节点累积等方面的研究进展，概述了构建嗜甲烷菌人工细胞的方法和提高甲烷同化效率的策略。同时介绍了基因组学、转录组学、代谢组学等组学研究方法在调控嗜甲烷菌底盘碳代谢流向和通量中的应用。最后，结合生物转化甲烷合成酸类、萜类、醇类等化学品的研究，分析并展望了嗜甲烷菌工业化应用所面临的挑战和机遇。

Abstract

Methane has been considered as a potential carbon source in industrial biotechnology because of its abundance

sustainability

high reducibility

and microbial availability. The biological conversion of methane into chemicals or fuels does not only reduce greenhouse gas emissions

but also substitute food-based substrates used in bio-manufacturing. Methanotrophs are gram-negative bacteria

and most are isolated from methane-plentiful environments. Owing to the presence of the methane monooxygenase

methanotrophs constitute a unique group of microbes. As an important industrially-promising microorganism with the characteristics of robust and anti-contamination ability

methanotrophs capable of growing with methane as the sole energy and carbon source play a significant role in carbon-neutral society by replacing petroleum-based products with biosynthesized products. Therefore

studies on methanotrophs for the biological conversion of methane have attracted extensive attention in recent years. With the rapid development of genetic manipulations tools and strategies for metabolically-engineered methanotrophs construction

including gene editing methods

regulation of metabolic pathways

and bio-elements mining

methanotrophic cell factories have been employed to efficiently convert methane into a variety of bulk chemicals and biofuels. In this review

biosynthetic technologies related to bioconversion of under-utilized methane ranging from fundamental understanding

systematic analysis

metabolic engineering to bio-product production are introduced. The genetic manipulations tools of methanotrophs

the approaches of methanotrophic cell factory construction

and the enhancement of methane assimilation efficiency are summarized from the aspects including the research progress of genetic engineering of methanotrophs

the regulation of methane carbon flux

the overexpression of heterologous pathway genes

and the accumulation of metabolic intermediates. Besides

the applications of genomics

transcriptomics

metabolomics

and metabolic modeling have been also deployed to facilitate the methane metabolism in methanotrophs chassis. Finally

given the strategy of 'waste-to-value' production

the challenges and opportunities for methane bioconversion by methanotrophs are also discussed and prospected based on industrial applications in terms of the research progress in the biosynthesis of methane-based acids

terpenes

alcohols

and other chemicals.

关键词

Keywords

references

FEI Q , PIENKOS P T . Bioconversion of methane for value-added products [M ] //SANI R K, RATHINAM N K. Extremophilic microbial processing of lignocellulosic feedstocks to biofuels , value-added products, and usable power. Cham : Springer , 2018 : 145 - 162 .

YVON-DUROCHER G , ALLEN A P , BASTVIKEN D , et al . Methane fluxes show consistent temperature dependence across microbial to ecosystem scales [J ] . Nature , 2014 , 507 ( 7493 ): 488 - 491 .

EPA . Understanding global warming potentials, 2017 [EB/OL ] . https://www.epa.gov/ghgemissions/understanding-global-warming-potentials https://www.epa.gov/ghgemissions/understanding-global-warming-potentials .

FELDMAN D R , COLLINS W D , BIRAUD S C , et al . Observationally derived rise in methane surface forcing mediated by water vapour trends [J ] . Nature Geoscience , 2018 , 11 ( 4 ): 238 - 243 .

BOUSQUET P , CIAIS P , MILLER J B , et al . Contribution of anthropogenic and natural sources to atmospheric methane variability [J ] . Nature , 2006 , 443 ( 7110 ): 439 - 443 .

EPA . Inventory of U.S. Greenhouse gas emissions and sinks : 1990 - 2013 , 2015[EB/OL ] . https://www.epa.gov/ghgemissions/inventory-us-greenhouse-gas-emissions-and-sinks https://www.epa.gov/ghgemissions/inventory-us-greenhouse-gas-emissions-and-sinks .

HWANG I Y , HUR D H , LEE J H , et al . Batch conversion of methane to methanol using Methylosinus trichosporium OB3b as biocatalyst [J ] . Journal of Microbiology and Biotechnology , 2015 , 25 ( 3 ): 375 - 380 .

KALYUZHNAYA M G , PURI A W , LIDSTROM M E . Metabolic engineering in methanotrophic bacteria [J ] . Metabolic Engineering , 2015 , 29 : 142 - 152 .

胡礼珍 , 王佳 , 袁波 , 等 . 碳一气体生物利用进展 [J ] . 生物加工过程 , 2017 , 15 ( 6 ): 17 - 25 .

HU L Z , WANG J , YUAN B , et al . Production of biofuels and chemicals from C 1 gases by microorganisms: status and prospects [J ] . Chinese Journal of Bioprocess Engineering , 2017 , 15 ( 6 ): 17 - 25 .

LIU Y C , HE X R , ZHU P P , et al . pheS AG based rapid and efficient markerless mutagenesis in Methylotuvimicrobium [J ] . Frontiers in Microbiology , 2020 , 11 : 441 .

JEON Y C , ANH D N , LEE E Y . Bioproduction of Isoprenoids and other secondary metabolites using methanotrophic bacteria as an alternative microbial cell factory option: current stage and future aspects [J ] . Catalysts , 2019 , 9 ( 11 ): 883 .

CANTERA S , BORDEL S , LEBRERO R , et al . Bio-conversion of methane into high profit margin compounds: an innovative, environmentally friendly and cost-effective platform for methane abatement [J ] . World Journal of Microbiology and Biotechnology , 2019 , 35 ( 1 ): 16 .

NGUYEN A D , LEE E Y . Engineered methanotrophy: a sustainable solution for methane-based industrial biomanufacturing [J ] . Trends in Biotechnology , 2021 , 39 ( 4 ): 381 - 396 .

NGUYEN A D , HWANG I Y , LEE O K , et al . Systematic metabolic engineering of Methylomicrobium alcaliphilum 20Z for 2,3-butanediol production from methane [J ] . Metabolic Engineering , 2018 , 47 : 323 - 333 .

NGUYEN D T N , LEE O K , LIM C , et al . Metabolic engineering of type Ⅱ methanotroph, Methylosinus trichosporium OB3b, for production of 3-hydroxypropionic acid from met hane via a malonyl-CoA reductase-dependent pathway [J ] . Metabolic Engineering , 2020 , 59 : 142 - 150 .

FU Y F , LI Y , LIDSTROM M . The oxidative TCA cycle operates during methanotrophic growth of the Type I methanotroph Methylomicrobium buryatense 5GB1 [J ] . Metabolic Engineering , 2017 , 42 : 43 - 51 .

陆吉学 , 王世珍 , 方柏山 . 生物分子机器——甲烷单加氧酶的研究进展 [J ] . 生物工程学报 , 2015 , 31 ( 7 ): 1015 - 1023 .

LU J X , WANG S Z , FANG B S , et al . Advances in biomolecular machine: methane monooxygenases [J ] . Chinese Journal of Biotechnology , 2015 , 31 ( 7 ): 1015 - 1023 .

韩冰 , 苏涛 , 李信 , 等 . 甲烷氧化菌及甲烷单加氧酶的研究进展 [J ] . 生物工程学报 , 2008 , 24 ( 9 ): 1511 - 1519 .

HAN B , SU T , LI X , et al . Research progresses of methanotrophs and methane monooxygenases [J ] . Chinese Journal of Biotechnology , 2008 , 24 ( 9 ): 1511 - 1519 .

KO Y S , KIM J W , LEE J A , et al . Tools and strategies of systems metabolic engineering for the development of microbial cell factories for chemical production [J ] . Chemical Society Reviews , 2020 , 49 ( 14 ): 4615 - 4636 .

LEE O K , NGUYEN D T N , LEE E Y . Metabolic engineering of methanotrophs for the production of chemicals and fuels [M ] // LEE E Y. Methanotrophs . Cham : Springer , 2019 : 163 - 203 .

CHOI K R , JANG W D , YANG D , et al . Systems metabolic engineering strategies: integrating systems and synthetic biology with metabolic engineering [J ] . Trends in Biotechnology , 2019 , 37 ( 8 ): 817 - 837 .

ANTONIEWICZ M R . Synthetic methylotrophy: strategies to assimilate methanol for growth and chemicals production [J ] . Current Opinion in Biotechnology , 2019 , 59 : 165 - 174 .

HWANG I Y , NGUYEN A D , NGUYEN T T , et al . Biological conversion of methane to chemicals and fuels: technical challenges and issues [J ] . Applied Microbiology and Biotechnology , 2018 , 102 ( 7 ): 3071 - 3080 .

CLOMBURG J M , CRUMBLEY A M , GONZALEZ R . Industrial biomanufacturing: the future of chemical production [J ] . Science , 2017 , 355 : aag08046320 .

FEI Q , GUARNIERI M T , TAO L , et al . Bioconversion of natural gas to liquid fuel: opportunities and challenges [J ] . Biotechnology Advances , 2014 , 32 ( 3 ): 596 - 614 .

MURRELL J C , JETTEN M S M . The microbial methane cycle [J ] . Environmental Microbiology Reports , 2009 , 1 ( 5 ): 279 - 284 .

LEE O K , HUR D H , NGUYEN D T N , et al . Metabolic engineering of methanotrophs and its application to production of chemicals and biofuels from methane [J ] . Biofuels, Bioproducts and Biorefining , 2016 , 10 ( 6 ): 848 - 863 .

SEMRAU J D , DISPIRITO A A , YOON S . Methanotrophs and copper [J ] . FEMS Microbiology Reviews , 2010 , 34 : 496 - 531 .

NARIYA S , KALYUZHNAYA M G . Diversity, physiology, and biotechnological potential of halo(alkali)philic methane-consuming bacteria [M ] // LEE E Y. Methanotrophs . Cham : Springer , 2019 : 139 - 161 .

郭树奇 , 费强 . 甲烷生物利用及嗜甲烷菌的工程改造 [J ] . 生物工程学报 , 2021 , 37 ( 3 ): 816 - 830 .

GUO S Q , FEI Q . Bioconversion of methane by metabolically engineered methanotrophs [J ] . Chinese Journal of Biotechnology , 2021 , 37 ( 3 ): 816 - 830 .

GROOM J D , FORD S M , PESESKY M W , et al . A mutagenic screen identifies a tonb-dependent receptor required for the lanthanide metal switch in the type I methanotroph " Methylotuvimicrobium buryatense " 5GB1C [J ] . Journal of Bacteriology , 2019 , 201 ( 15 ): e00120-19 .

LEE J K , KIM S , KIM W , et al . Efficient production of D-lactate from methane in a lactate-tolerant strain of Methylomonas sp. DH-1 generated by adaptive laboratory evolution [J ] . Biotechnology for Biofuels , 2019 , 12 : 234 .

GARG S , CLOMBURG J M , GONZALEZ R . A modular approach for high-flux lactic acid production from methane in an industrial medium using engineered Methylomicrobium buryatense 5GB1 [J ] . Journal of Industrial Microbiology & Biotechnology , 2018 , 45 ( 6 ): 379 - 391 .

PURI A W , OWEN S , CHU F , et al . Genetic tools for the industrially promising methanotroph Methylomicrobium buryatense [J ] . Applied and Environmental Microbiology , 2015 , 81 ( 5 ): 1775 - 1781 .

SIRAJUDDIN S , ROSENZWEIG A C . Enzymatic oxidation of methane [J ] . Biochemistry , 2015 , 54 ( 14 ): 2283 - 2294 .

SHARPE P L , DICOSIMO D , BOSAK M D , et al . Use of transposon promoter-probe vectors in the metabolic engineering of the obligate methanotroph Methylomonas sp. strain 16a for enhanced C40 carotenoid synthesis [J ] . Applied and Environmental Microbiology , 2007 , 73 ( 6 ): 1721 - 1728 .

HENARD C A , SMITH H , DOWE N , et al . Bioconversion of methane to lactate by an obligate methanotrophic bacterium [J ] . Scientific Reports , 2016 , 6 : 21585 .

CSÁKI R , BODROSSY L , KLEM J , et al . Genes involved in the copper-dependent regulation of soluble methane monooxygenase of Methylococcus capsulatus (Bath): cloning, sequencing and mutational analysis [J ] . Microbiology (Reading) , 2003 , 149 ( 7 ): 1785 - 1795 .

NGUYEN A D , CHAU T H T , LEE E Y . Methanotrophic microbial cell factory platform for simultaneous conversion of methane and xylose to value-added chemicals [J ] . Chemical Engineering Journal , 2021 , 420 : 127632 .

TAO L , SEDKOVA N , YAO H , et al . Expression of bacterial hemoglobin genes to improve astaxanthin production in a methanotrophic bacterium Methylomonas sp .[J ] . Applied Microbiology and Biotechnology , 2007 , 74 ( 3 ): 625 - 633 .

YAN X , CHU F , PURI A W , et al . Electroporation-based genetic manipulation in type I Methanotrophs [J ] . Applied and Environmental Microbiology , 2016 , 82 ( 7 ): 2062 - 2069 .

TAPSCOTT T , MICHAEL T G , HENARD C A . Development of a CRISPR/Cas9 system for Methylococcus capsulatus in vivo gene editing .[J ] . Applied and Environmental Microbiology , 2019 , 85 ( 11 ): e00340-19 .

KHMELENINA V N , BECK D A C , MUNK C , et al . Draft genome sequence of Methylomicrobium buryatense strain 5G, a haloalkaline-tolerant methanotrophic bacterium [J ] . Genome Announcements , 2013 , 1 ( 4 ): e00053-13 .

GARG S , WU H , CLOMBURG J M , et al . Bioconversion of methane to C-4 carboxylic acids using carbon flux through acetyl-CoA in engineered Methylomicrobium buryatense 5GB1C [J ] . Metabolic Engineering , 2018 , 48 : 175 - 183 .

HENARD C A , AKBERDIN I R , KALYUZHNAYA M G , et al . Muconic acid production from methane using rationally-engineered methanotrophic biocatalysts [J ] . Green Chemistry , 2019 , 21 ( 24 ): 6731 - 6737 .

DEMIDENKO A , AKBERDIN I R , ALLEMANN M , et al . Fatty acid biosynthesis pathways in Methylomicrobium buryatense 5G(B1) [J ] . Frontiers in Microbiology , 2017 , 7 : 2167 .

VUILLEUMIER S , KHMELENINA V N , BRINGEL F , et al . Genome sequence of the haloalkaliphilic methanotrophic bacterium Methylomicrobium alcaliphilum 20Z [J ] . Journal of Bacteriology , 2012 , 194 ( 2 ): 551 - 552 .

NGUYEN A D , KIM D , LEE E Y . Unlocking the biosynthesis of sesquiterpenoids from methane via the methylerythritol phosphate pathway in methanotrophic bacteria, using α -humulene as a model compound [J ] . Metabolic Engineering , 2020 , 61 : 69 - 78 .

NGUYEN L T , LEE E Y . Biological conversion of methane to putrescine using genome-scale model-guided metabolic engineering of a methanotrophic bacterium Methylomicrobium alcaliphilum 20Z [J ] . Biotechnology for Biofuels , 2019 , 12 ( 1 ): 147 .

HENARD C A , FRANKLIN T G , YOUHENNA B , et al . Biogas biocatalysis: methanotrophic bacterial cultivation, metabolite profiling, and bioconversion to lactic acid [J ] . Frontiers in Microbiology , 2018 , 9 : 2610 .

WARD N , LARSEN Q , SAKWA J , et al . Genomic Insights into methanotrophy: the complete genome sequence of Methylococcus capsulatus (Bath) [J ] . PLoS Biology , 2004 , 2 ( 10 ): 1616 - 1628 .

LEONARD E , MINSHULL J , NESS J , et al . Compositions and methods for biological production of isoprene : USWO/2014/138419 [P ] . 2014-09-12 .

HELD M A , ZGAO X , CHAO L Y , et al . New genetically modified microorganism comprising a heterologous gene under the control of a molecular switch , useful for making a multicarbon product , 2 , 3 -butanediol, 1 , 4 -butanediol, and isobutyraldehyde: US 0040344 [P ] . 2020-02-06 .

COLEMAN W J , VIDANES G M , COTTAREL G , et al . Biological conversion of multi-carbon compounds from methane : US20140273128 [P ] . 2014-09-18 .

NGUYEN A D , HWANG I Y , LEE O K , et al . Functional analysis of Methylomonas sp. DH-1 genome as a promising biocatalyst for bioconversion of methane to valuable chemicals [J ] . Catalysts , 2018 , 8 ( 3 ): 117 .

NGUYEN D T N , LEE O K , HADIYATI S , et al . Metabolic engineering of the type Ⅰ methanotroph Methylomonas sp. DH-1 for production of succinate from methane [J ] . Metabolic Engineering , 2019 , 54 : 170 - 179 .

STEIN L Y , YOON S , SEMRAU J D , et al . Genome sequence of the obligate methanotroph Methylosinus trichosporium strain OB3b [J ] . Journal of Bacteriology , 2010 , 192 ( 24 ): 6497 - 6498 .

THINGUYEN T , KYUNGLEE O , SANZHARNAIZABEKOV , et al . Bioconversion of methane to cadaverine and lysine using an engineered type Ⅱ methanotroph, Methylosinus trichosporium OB3b [J ] . Green Chemistry , 2020 , 22 , 7803 - 7811 .

DICOSIMO D J , KOFFAS M , ODOM J M , et al . Production of cyclic terpenoids : US6818424 [P ] . 2004-11-16 .

YE R W , YAO H , STEAD K , et al . Construction of the astaxanthin biosynthetic pathway in a methanotrophic bacterium Methylomonas sp strain 16a [J ] . Journal of Industrial Microbiology Biotechnology , 2007 , 34 ( 4 ): 289 - 299 .

HENARD C A , SMITH H K , GUARNIERI M T . Phosphoketolase overexpression increases biomass and lipid yield from methane in an obligate methanotrophic biocatalyst [J ] . Metabolic Engineering , 2017 , 41 : 152 - 158 .

BOGORAD I W , LIN T S , LIAO J C . Synthetic non-oxidative glycolysis enables complete carbon conservation [J ] . Nature , 2013 , 502 ( 7473 ): 693 - 697 .

CHINEN A , KOZLOV Y I , HARA Y , et al . Innovative metabolic pathway design for efficient l -glutamate production by suppressing CO 2 emission [J ] . Journal of Bioscience and Bioengineering , 2007 , 103 ( 3 ): 262 - 269 .

HENARD C A , FREED E F , GUARNIERI M T . Phosphoketolase pathway engineering for carbon-efficient biocatalysis [J ] . Current Opinion in Biotechnology , 2015 , 36 : 183 - 188 .

MEADOWS A L , HAWKINS K M , TSEGAYE Y , et al . Rewriting yeast central carbon metabolism for industrial isoprenoid production [J ] . Nature , 2016 , 537 ( 7622 ): 694 - 697 .

NIU F X , LU Q , BU Y F , et al . Metabolic engineering for the microbial production of isoprenoids: carotenoids and isoprenoid-based biofuels [J ] . Synthetic and Systems Biotechnology , 2017 , 2 ( 3 ): 167 - 175 .

AKBERDIN I R , THOMPSON M , HAMILTON R , et al . Methane utilization in Methylomicrobium alcaliphilum 20Z(R): a systems approach [J ] . Scientific Reports , 2018 , 8 ( 1 ): 2512 .

SUN L , GONG M Y , LÜ X , et al . Current advance in biological production of short-chain organic acid [J ] . Applied Microbiology and Biotechnology , 2020 , 104 ( 21 ): 9109 - 9124 .

MCANULTY M J , POOSARLA V G , LI J , et al . Metabolic engineering of Methanosarcina acetivorans for lactate production from methane [J ] . Biotechnology and Bioengineering , 2017 , 114 ( 4 ): 852 - 861 .

KIM J W , KO Y S , CHAE T U , et al . High-level production of 3-hydroxypropionic acid from glycerol as a sole carbon source using metabolically engineered Escherichia coli [J ] . Biotechnology and Bioengineering , 2020 , 117 ( 7 ): 2139 - 2152 .

WANG L , ZONG Z , LIU Y , et al . Metabolic engineering of Y arrowia lipolytica for the biosynthesis of crotonic acid [J ] . Bioresource Technology , 2019 , 287 : 121484 .

AHN J H , SEO H , PARK W , et al . Enhanced succinic acid production by mannheimia employing optimal malate dehydrogenase [J ] . Nature Communications , 2020 , 11 ( 1 ): 1970 .

MENG X , YANG J M , CAO Y J , et al . Increasing fatty acid production in E. coli by simulating the lipid accumulation of oleaginous microorganisms [J ] . Journal of Industrial Microbiology Biotechnology , 2011 , 38 ( 8 ): 919 - 925 .

ALBER B E . Biotechnological potential of the ethylmalonyl-CoA pathway [J ] . Applied Microbiology and Biotechnology , 2011 , 89 ( 1 ): 17 - 25 .

DAVIS M S , CRONAN J E . Inhibition of Escherichia coli acetyl coenzyme a carboxylase by acyl-acyl carrier protein [J ] . Journal of Bacteriology , 2001 , 183 ( 4 ): 1499 - 1503 .

KIM S , CHEONG S , GONZALEZ R . Engineering Escherichia coli for the synthesis of short-and medium-chain α,β -unsaturated carboxylic acids [J ] . Metabolic Engineering , 2016 , 36 : 90 - 98 .

CLOMBURG J M , VICK J E , BLANKSCHIEN M D , et al . A synthetic biology approach to engineer a functional reversal of the β -oxidation cycle [J ] . ACS Synthetic Biology , 2012 , 1 ( 11 ): 541 - 554 .

AKBERDIN I R , COLLINS D A , HAMILTON R , et al . Rare earth elements alter redox balance in Methylomicrobium alcaliphilum 20Z(R) [J ] . Frontiers in Microbiology , 2018 , 9 : 2735 .

BORDEL S R Y H . Genome scale metabolic modeling reveals the metabolic potential of three Type II methanotrophs of the genus Methylocystis [J ] . Metabolic Engineering , 2019 , 54 : 191 - 199 .

FU Y , HE L , REEVE J , et al . Core metabolism shifts during growth on methanol versus methane in the methanotroph Methylomicrobium buryatense 5GB1 [J ] . mBio , 2019 , 10 ( 2 ): e00406-19 .

NGUYEN A D , PARK J Y , HWANG I Y , et al . Genome-scale evaluation of core one-carbon metabolism in gammaproteobacterial methanotrophs grown on methane and methanol [J ] . Metabolic Engineering , 2020 , 57 : 1 - 12 .

HU L Z , YANG Y , YAN X , et al . Molecular mechanism associated with the impact of methane/oxygen gas supply ratios on cell growth of Methylomicrobium buryatense 5GB1 through RNA-Seq [J ] . Frontiers in Bioengineering and Biotechnology , 2020 , 8 : 263 .

HAKOBYAN A , ZHU J , GLATTER T , et al . Hydrogen utilization by Methylocystis sp. strain SC2 expands the known metabolic versatility of type IIa methanotrophs [J ] . Metabolic Engineering , 2020 , 61 : 181 - 196 .

FEI Q , PURI A W , SMITH H , et al . Enhanced biological fixation of methane for microbial lipid production by recombinant Methylomicrobium buryatense [J ] . Biotechnology for Biofuels , 2018 , 11 : 129 .

DONALDSON G K , HOLLANDS K , PICATAGGIO S K . Biocatalyst for conversion of methane and methanol to isoprene : US20150225743 [P ] . 2015-08-13 .

REN Y Y , LIU S S , JIN G J , et al . Microbial production of limonene and its derivatives: achievements and perspectives .[J ] . Biotechnology Advances , 2020 , 15 ( 44 ): 107628 .

ALEMDAR S , HARTWIG S , FRISTER T , et al . Heterologous expression, purification, and biochemical characterization of α -humulene synthase from Zingiber zerumbet Smith [J ] . Applied Biochemistry and Biotechnology , 2016 , 178 ( 3 ): 474 - 489 .

GUO W , LI D , HE R , et al . Synthesizing value-added products from methane by a new Methylomonas [J ] . Journal of Applied Microbiology , 2017 , 123 ( 5 ): 1214 - 1227 .

LIANG L Y , LIU R M , FREED E F , et al . Synthetic biology and metabolic engineering employing Escherichia coli for C 2 -C 6 bioalcohol production [J ] . Frontiers in Bioengineering and Biotechnology , 2020 , 8 : 710 .

JANG Y S , KIM B , SHIN J H , et al . Bio-based production of C 2 -C 6 platform chemicals [J ] . Biotechnology Bioengineering , 2012 , 109 ( 10 ): 2437 - 2459 .

BALSKUS E P , WALSH C T . The genetic and molecular basis for sunscreen biosynthesis in cyanobacteria [J ] . Science , 2010 , 329 ( 5999 ): 1653 - 1656 .

PARK S H , LEE K , JANG J W , et al . Metabolic engineering of Saccharomyces cerevisiae for production of shinorine, a sunscreen material, from xylose [J ] . ACS Synthetic Biology , 2019 , 8 ( 2 ): 346 - 357 .

GONZALEZ J E , LONG C P , ANTONIEWICZ M R . Comprehensive analysis of glucose and xylose metabolism in Escherichia coli under aerobic and anaerobic conditions by 13 C metabolic flux analysis [J ] . Metabolic Engineering , 2017 , 39 : 9 - 18 .

GAO J Y , YOU F Q . Design and optimization of shale gas energy systems: overview, research challenges, and future directions [J ] . Computers Chemical Engineering , 2017 , 106 ( 11 ): 699 - 718 .

王红秋 , 乔明 , 郑轶丹 . 美“页岩气化工”重塑全球化工产业链 [J ] . 中国石油企业 , 2017 ( 3 ): 73 - 74 .

WANG H Q , QIAO M , ZHENG Y D . The "shale gas chemical industry" of USA reshapes global chemical industry chain [J ] . China Petroleum Enterprise , 2017 ( 3 ): 73 - 74 .

金瑞庭 . 当前国际大宗商品价格走势及2020年展望 [J ] . 中国经贸导刊 , 2020 ( 9 ): 24 - 25 .

JIN R T . Current international commodity price trends and prospects for 2020 [J ] . China Economic Trade Herald , 2020 ( 9 ): 24 - 25 .

KALYUZHNAYA M G , YANG S , ROZOVA O N , et al . Highly efficient methane biocatalysis revealed in a methanotrophic bacterium [J ] . Nature Communications , 2013 , 4 : 2785 .

NAIZABEKOV S , LEE E Y . Genome-scale metabolic model reconstruction and in silico investigations of methane metabolism in Methylosinus trichosporium OB3b [J ] . Microorganisms , 2020 , 8 ( 3 ): 437 .

高子熹 , 郭树奇 , 费强 . 生物转化温室气体生产单细胞蛋白的研究进展 [J ] . 化工学报 , 2021 , 72 ( 6 ): 3202 - 3214 .

GAO Z X , GUO S Q , FEI Q . Recent progress in microbial bioconversion of greenhouse gases into single cell protein [J ] . CIESC Journal , 2021 , 72 ( 6 ): 3202 - 3214 .

PICONE N , MOHAMMADI S S , WAAJEN A C , et al . More than a methanotroph: a broader substrate spectrum for Methylacidiphilum fumariolicum SolV [J ] . Frontiers in Microbiology , 2020 , 11 : 604485 .

REN J , LEE H M , THAI T D , et al . Identification of a cytosine methyltransferase that improves transformation efficiency in Methylomonas sp. DH-1 [J ] . Biotechnology for Biofuels , 2020 , 13 ( 1 ): 200 .

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