人造蛋白功能材料的生物合成及应用

曾丹; 储建林; 陈燕茹; 范代娣

doi:10.12211/2096-8280.2020-091

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人造蛋白功能材料的生物合成及应用

特约评述 | 更新时间：2026-03-05

- 人造蛋白功能材料的生物合成及应用
- Biological synthesis and applications of artificial protein functional materials
- 合成生物学 2021年2卷第4期页码：528-542
- 作者机构：
  
  1.西北大学化工学院，陕西省可降解生物医用材料重点实验室，陕西省生物材料与发酵工程技术研究中心，陕西西安 710069
  2.南京工业大学药学院，江苏南京 210000
- 作者简介：
  
  [ "曾丹（1989—），女，博士，副教授。主要研究方向为生物医用材料。E-mail：zengdan0301@nwu.edu.cn" ]
  [ "范代娣（1965—），女，博士，教授。主要研究方向为可降解生物材料，预防医学和营养医学。E-mail：fandaidi@nwu.edu.cn" ]
- 基金信息：
  
  国家重点研发计划“合成生物学”重点专项;人造蛋白质合成的细胞设计构建及应用(2019YFA0905200)
- DOI：10.12211/2096-8280.2020-091
  中图分类号： Q816
- 收稿：2020-12-28，
  
  修回：2021-05-29，
  
  纸质出版：2021-08-31
- 稿件说明：
移动端阅览
曾丹，储建林，陈燕茹，范代娣. 人造蛋白功能材料的生物合成及应用［J］. 合成生物学， 2021， 2（4）： 528-542

ZENG Dan， CHU Jianlin， CHEN Yanru， FAN Daidi. Biological synthesis and applications of artificial protein functional materials［J］. Synthetic Biology Journal， 2021， 2（4）： 528-542
曾丹，储建林，陈燕茹，范代娣. 人造蛋白功能材料的生物合成及应用［J］. 合成生物学， 2021， 2（4）： 528-542 DOI： 10.12211/2096-8280.2020-091.

ZENG Dan， CHU Jianlin， CHEN Yanru， FAN Daidi. Biological synthesis and applications of artificial protein functional materials［J］. Synthetic Biology Journal， 2021， 2（4）： 528-542 DOI： 10.12211/2096-8280.2020-091.

摘要

人造蛋白功能材料具有良好的生物相容性和生物可降解性，来源广泛且功能多样，是一类理想的生物材料，在生物工程、医药、军事和纺织等领域具有广泛的应用前景。然而，现阶段蛋白功能材料的微生物合成仍存在表达量低、性能不稳定等问题，严重限制了这些蛋白材料的高效生产与应用。合成生物学在工程化理念的指导下，为人造蛋白功能材料提供了“精准设计—系统构建—调控表达—工程应用”的研究策略。本文介绍了蛛丝蛋白、蚕丝蛋白、类人胶原蛋白和贻贝蛋白等主要蛋白功能材料的生物合成研究进展，并阐述了人造蛋白细胞工厂的构建、蛋白的调控表达和其在组织工程材料等领域的应用现状。具有动态响应特性的人造蛋白功能材料是未来的研究方向。

Abstract

Artificial protein functional materials have good biocompatibility

biodegradability

broad resources of raw materials

and diverse functions. As ideal biological materials

they have a wide range of application prospects in bioengineering

medicine

military and textiles. However

microbial synthesis of protein functional materials still has problems such as low expression and unstable performance

which severely restrict their efficient production and application. In recent years

there is a trend of designing artificial protein functional materials that not only possess multi-functional properties but are biomimetic

adaptive and dynamically responsive to biological processes

and such a trend puts forward new requirements for the design and engineering control of macromolecular systems

which raise a necessity for developing research methods to assemble engineered protein molecules into functional macroscopic materials. Under the guidance of engineering concepts

synthetic biology provides a strategy for developing "precise design-system construction-regulatory expres

sion-engineering application" for artificial protein functional materials. This review comments the research progress in the biological synthesis of major protein functional materials such as spider silk protein

silk protein

human-like collagen and mussel protein

and explores the construction of artificial protein cell factory

the regulation of protein expression and their applications as tissue engineering materials and other fields. In view of the current problems of poor biocompatibility and insufficient efficacy of clinical products

artificial protein functional materials can be assembled and processed according to functional requirements

and used to fabricate artificial tendons

artificial skin

degradable hemostatic materials

artificial bones and new protein material medical products such as high-viscosity anti-fouling coating products. There are still several challenges that need to be solved urgently: (1) There are few theoretical analysis tools and models at this stage

making the design

prediction and analysis of functional proteins relatively limited

and a more comprehensive protein database needs to be established; (2) There are many expression regulators in

E. coli

and other microbial systems

and adaptation for the design of expression elements and the target gene

the regulation of protein synthesis pathways and the systemic nature need to be studied to improve the efficiency of protein expression; (3) In terms of engineering applications

it is also necessary to comprehensively consider material stability and biological safety. Finally

the research direction is prospected

which provides insights for scholars working in related fields.

关键词

Keywords

references

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