DNA微阵列原位化学合成

闫汉; 肖鹏峰; 刘全俊; 陆祖宏

doi:10.12211/2096-8280.2020-089

您当前的位置：

首页 >

文章列表页 >

DNA微阵列原位化学合成

特约评述 | 更新时间：2025-03-20

- DNA微阵列原位化学合成
- In situ chemical synthesis of DNA microarrays
- 合成生物学 2021年2卷第3期页码：354-370
- 作者机构：
  
  东南大学生物科学与生物医学工程学院，生物电子学国家重点实验室，江苏南京 210096
- 作者简介：
  
  [ "闫汉（1991—），男，博士研究生。研究方向为DNA合成自组装，纳米孔生物分子检测。E-mail：230209118@seu.deu.cn" ]
  [ "刘全俊（1968—），男，教授，博士生导师。研究方向为新一代基因测序、基因芯片、生物与化学传感器、单分子检测。E-mail：lqj@seu.edu.cn" ]
  [ "陆祖宏（1960—），男，教授，博士生导师。研究方向为生物电子学，超分子组装、生物传感器、生物信息技术等。 E-mail：zhl@seu.deu.cn" ]
- 基金信息：
  
  国家自然科学基金(60121101;61971123;61827814;31872726)
- DOI：10.12211/2096-8280.2020-089
  中图分类号： Q81
- 收稿：2020-12-21，
  
  修回：2021-04-09，
  
  纸质出版：2021-06-30
- 稿件说明：
移动端阅览
闫汉，肖鹏峰，刘全俊，陆祖宏. DNA微阵列原位化学合成［J］. 合成生物学， 2021， 2（3）： 354-370

YAN Han， XIAO Pengfeng， LIU Quanjun， LU Zuhong. In situ chemical synthesis of DNA microarrays［J］. Synthetic Biology Journal， 2021， 2（3）： 354-370
闫汉，肖鹏峰，刘全俊，陆祖宏. DNA微阵列原位化学合成［J］. 合成生物学， 2021， 2（3）： 354-370 DOI： 10.12211/2096-8280.2020-089.

YAN Han， XIAO Pengfeng， LIU Quanjun， LU Zuhong. In situ chemical synthesis of DNA microarrays［J］. Synthetic Biology Journal， 2021， 2（3）： 354-370 DOI： 10.12211/2096-8280.2020-089.

摘要

高通量、快速、低成本DNA合成是合成生物学、DNA信息存储以及DNA芯片等前沿科技领域的重要核心技术。DNA微阵列原位化学合成方法是在亚磷酸酰胺固相化学合成原理的基础上，整合了微电子学、计算科学、分子生物学、光电化学和微纳加工等学科的相关技术，近30年来得到了迅速的发展和应用。DNA微阵列原位化学合成方法根据不同的碱基分配方式可以分为原位光刻法、光敏抗蚀层合成法、光致酸法、喷印合成法、软光刻合成法、电致酸法和压印法以及以这些技术为基础衍生的各种合成方法等。本文对上述不同的DNA微阵列原位化学合成方法及其技术特点进行阐述，并对未来DNA合成方法的发展趋势进行讨论和展望。合成通量和效率方面基于CMOS芯片的电致酸DNA原位化学合成技术在未来10年内将具备较大的发展空间，通过解决芯片上微电极间氢离子串扰问题，有望实现单片TB级的DNA快速低成本合成。

Abstract

High-throughput

rapid and low-cost DNA synthesis is an important core technology in the research fields such as synthetic biology

DNA storage and DNA chips. The in-situ chemical synthesis of DNA microarrays is based on the principle of solid-phase chemical synthesis of phosphorous acid amides

and integrates the relevant technologies of microelectronics

computational science

molecular biology

photo-electrochemistry and micro-nano processing. In the past 30 years

the technology has developed rapidly.

situ

chemical synthesis methods can be grouped into photolithography

photo-acid methods

electro-acid methods

printing methods and imprinting methods

etc

. based on their base allocation strategy. In this paper

we discuss the different

in-situ

chemical synthesis methods of DNA microarrays and their technical characteristics

as well as the potential development trends of DNA synthesis methods in the future. We believe that in terms of synthesis throughput and efficiency

the CMOS (Complementary Metal Oxide Semiconductor) chip-based in-situ chemical synthesis of electro-acid DNA has tremendous potential in the next decade. By solving the problem of hydrogen ion crosstalk between microelectrodes on the chip

it is expected that the rapid and low-cost synthesis of TB-level DNA can be accomplished on a single chip. As shown in the schematic diagram

the voltage of different areas of the DNA synthesis chip is controlled by computer to selectively deprotect the bases at different sites to achieve high-throughput parallel synthesis of different sequences of DNA. However

the bottleneck comes from the crosstalk between micro-electrodes due to acidic ion diffusion inside CMOS chip

which results in the limitation of synthetic capacity at the hundreds-of-megabyte level. After studying the behavior of hydrogen ion transport at the micrometer scale

the redesigning of CMOS chip with new materials and structures is suggested

aiming to suppress the ion diffusion between micro-electrodes and optimize the structure of microelectrodes and microfluidic channels of the chip and device. It is possible that the synthetic capacity of single chip could reach terabyte-level for CMOS-based in situ parallel DNA chemical synthesis with electro-acidified deprotection in the future

which will significantly accelerate the practical applications of synthetic biology and related technologies.

关键词

Keywords

references

王冬梅 , 洪泂 . 从碱基到人造生命——基因组的从头合成 [J ] . 生命的化学 , 2011 , 31 ( 1 ): 13 - 20 .

WANG D M , HONG J . From base to artificial life—chemical synthesis of genome [J ] . Chemistry of Life , 2011 , 31 ( 1 ): 13 - 20 .

GIBSON D G , GLASS J I , LARTIGUE C , et al . Creation of a bacterial cell controlled by a chemically synthesized genome [J ] . Science , 2010 , 329 ( 5987 ): 52 - 56 .

李雷 , 姜卫红 , 覃重军 , 等 . 合成生物学使能技术的研究进展 [J ] . 中国科学: 生命科学 , 2015 , 45 ( 10 ): 950 - 968 .

LI L , JIANG W H , QIN Z J , et al . Recent advances in the enabling technologies for synthetic biology [J ] . SCIENTIA SINICA Vitae , 2015 , 45 ( 10 ): 950 - 968 .

李诗渊 , 赵国屏 , 王金 . 合成生物学技术的研究进展——DNA合成、组装与基因组编辑 [J ] . 生物工程学报 , 2017 , 33 ( 3 ): 343 - 360 .

LI S Y , ZHAO G P , WANG J . Enabling technologies in synthetic biology—DNA synthesis, assembly and editing [J ] . Chinese Journal of Biotechnology , 2017 , 33 ( 3 ): 343 - 360 .

陈婧 , 肖鹏峰 . 高通量DNA合成测序化学研究进展 [J ] . 生物过程 , 2012 , 2 ( 1 ): 1 - 6 .

CHEN J , XIAO P F . Advance in sequence chemistry of high-throughput DNA sequencing by synthesis [J ] . Bioprocess , 2012 , 2 ( 1 ): 1 - 6 .

ABRAMOVA T . Frontiers and approaches to chemical synthesis of oligodeoxyribonucleotides [J ] . Molecules , 2013 , 18 ( 1 ): 1063 - 1075 .

CARUTHERS M H . The chemical synthesis of DNA/RNA: our gift to science [J ] . The Journal of Biological Chemistry , 2013 , 288 ( 2 ): 1420 - 1427 .

REESE C B . Oligo-and poly-nucleotides: 50 years of chemical synthesis [J ] . Organic & Biomolecular Chemistry , 2005 , 3 ( 21 ): 3851 - 3868 .

ABRAMOVA T V , SILNIKOV V N . Synthesis and properties of carbohydrate-phosphate backbone-modified oligonucleotide analogues and nucleic acid mimetics [J ] . Russian Chemical Reviews , 2011 , 80 ( 5 ): 429 - 452 .

WATTS J K , COREY D R . Silencing disease genes in the laboratory and the clinic [J ] . The Journal of Pathology , 2012 , 226 ( 2 ): 365 - 379 .

HUGHES R A , ELLINGTON A D . Synthetic DNA synthesis and assembly: putting the synthetic in synthetic biology [J ] . Cold Spring Harbor Perspectives In Biology , 2017 , 9 ( 1 ): 1 - 17 .

MCDANIEL R , WEISS R . Advances in synthetic biology: on the path from prototypes to applications [J ] . Current Opinion Biotechnology , 2005 , 16 ( 4 ): 476 - 483 .

KOBAYASHI H , KAERN M , ARAKI M , et al . Programmable cells: interfacing natural and engineered gene networks [J ] . Proceedings of the National Academy of Sciences of the United States of America , 2004 , 101 ( 22 ): 8414 - 8419 .

TIAN J , GONG H , SHENG N , et al . Accurate multiplex gene synthesis from programmable DNA microchips [J ] . Nature , 2004 , 432 ( 7020 ): 1050 - 1054 .

GIBSON D G , BENDERS G A , ANDREWS-PFANNKOCH C , et al . Complete chemical synthesis, assembly, and cloning of a Mycoplasma genitalium genome [J ] . Science , 2008 , 319 ( 5867 ): 1215 - 1220 .

SMITH H O , HUTCHISON C A , PFANNKOCH C , et al . Generating a synthetic genome by whole genome assembly: PhiX174 bacteriophage from synthetic oligonucleotides [J ] . Proceedings of the National Academy of Sciences of the United States of America , 2003 , 100 ( 26 ): 15440 - 15445 .

CELLO J , PAUL A V , WIMMER E . Chemical synthesis of poliovirus cDNA: generation of infectious virus in the absence of natural template [J ] . Science , 2002 , 297 ( 5583 ): 1016 - 1018 .

CHAN L Y , KOSURI S , ENDY D . Refactoring bacteriophage T7 [J ] . Molecular Systems Biology , 2005 , 1 : 1 - 10 .

卢俊南 , 罗周卿 , 姜双英 , 等 . DNA的合成、组装及转移技术 [J ] . 中国科学院院刊 , 2018 , 33 ( 11 ): 1174 - 1183 .

LU J N , LUO Z Q , JIANG S Y , et al . Synthesis, assembly and transfer of DNA [J ] . Bulletin of Chinese Academy of Sciences , 2018 , 33 ( 11 ) : 1174 - 1183 .

ZHRINOV V V , RASIC D . 2018 semiconductor synthetic biology roadmap [EB/OL ] . [ 2021-05-24 ] . http://www.researchgate.net/publication/328812596_2018_Semiconductor_Synthetic_Biology_Roadmap http://www.researchgate.net/publication/328812596_2018_Semiconductor_Synthetic_Biology_Roadmap .

ERLICH Y , ZIELINSKI D , ZIELINSKI D . DNA Fountain enables a robust and efficient storage architecture [J ] . Science , 2017 , 355 ( 6328 ): 950 - 954 .

ORGANICK L , ANG S D , CHEN Y J , et al . Random access in large-scale DNA data storage [J ] . Nature Biotechnology , 2018 , 36 ( 3 ): 242 - 248 .

KIM J , BAE J H , BAYM M , et al . Metastable hybridization-based DNA information storage to allow rapid and permanent erasure [J ] . Nature Communications , 2020 , 11 ( 1 ): 5008 .

HAO M , QIAO H , GAO Y , et al . A mixed culture of bacterial cells enables an economic DNA storage on a large scale [J ] . Communications Biology , 2020 , 3 ( 1 ): 416 .

LEE H , WIEGAND D J , GRISWOLD K , et al . Photon-directed multiplexed enzymatic DNA synthesis for molecular digital data storage [J ] . Nature Communications , 2020 , 11 ( 1 ): 5246 .

CEZE L , NIVALA J , STRAUSS K . Molecular digital data storage using DNA [J ] . Nature Reviews Genetics , 2019 , 20 ( 8 ): 456 - 466 .

MEISER L C , ANTKOWIAK P L , KOCH J , et al . Reading and writing digital data in DNA [J ] . Nature Protocols , 2020 , 15 ( 1 ): 86 - 101 .

RUTTEN M G T A , VAANDRAGER F W , ELEMANS J A A W , et al . Encoding information into polymers [J ] . Nature Reviews Chemistry , 2018 , 2 ( 11 ): 365 - 381 .

CHEN Y J , TAKAHASHI C N , ORGANICK L , et al . Quantifying molecular bias in DNA data storage [J ] . Nature Communications , 2020 , 11 ( 1 ): 3264 .

HECKEL R , MIKUTIS G , GRASS R N . A characterization of the DNA data storage channel [J ] . Scientific Reports , 2019 , 9 ( 1 ): 9663 .

ANAVY L , VAKNIN I , ATAR O , et al . Data storage in DNA with fewer synthesis cycles using composite DNA letters [J ] . Nature Biotechnology , 2019 , 37 ( 10 ): 1229 - 1236 .

TABATABAEI S K , WANG B , ATHREYA N B M , et al . DNA punch cards for storing data on native DNA sequences via enzymatic nicking [J ] . Nature Communications , 2020 , 11 ( 1 ): 1742 .

KOSURI S , CHURCH G M . Large-scale de novo DNA synthesis: technologies and applications [J ] . Nature Methods , 2014 , 11 ( 5 ): 499 - 507 .

LIM H , CHO N , AHN J , et al . Highly selective retrieval of accurate DNA utilizing a pool of in situ-replicated DNA from multiple next-generation sequencing platforms [J ] . Nucleic Acids Research , 2018 , 46 ( 7 ): e40 .

KOSURI S , EROSHENKO N , LEPROUST E M , et al . Scalable gene synthesis by selective amplification of DNA pools from high-fidelity microchips [J ] . Nature Biotechnology , 2010 , 28 ( 12 ): 1295 - 1299 .

MATZAS M , STÄHLER P F , KEFER N , et al . High-fidelity gene synthesis by retrieval of sequence-verified DNA identified using high-throughput pyrosequencing [J ] . Nature Biotechnology , 2010 , 28 ( 12 ): 1291 - 1294 .

CLEARY M A , KILIAN K , WANG Y , et al . Production of complex nucleic acid libraries using highly parallel in situ o ligonucleotide synthesis [J ] . Nature Methods , 2004 , 1 ( 3 ): 241 - 248 .

LEE C C , SNYDER T M , QUAKE S R . A microfluidic oligonucleotide synthesizer [J ] . Nucleic Acids Research , 2010 . 38 ( 8 ): 2514 - 2521 .

MATTEUCCI M D , CARUTHERS M H . Synthesis of deoxyoligonucleotides on a polymer support [J ] . Journal of the American Chemical Society , 1981 , 103 ( 11 ): 3185 - 3191 .

TIAN J D , MA K S , SAAEM I . Advancing high-throughput gene synthesis technology [J ] . Molecular BioSystems , 2009 , 5 ( 7 ): 714 - 722 .

CARUTHERS M H , BEAUCAGE S L , BECKER C , et al . Deoxyoligonucleotide synthesis via the phosphoramidite method [J ] . Gene Amplification and Analysis , 1983 , 3 : 1 - 26 .

CARUTHERS M H , BARONE A D , BEAUCAGE S L , et al . Chemical synthesis of deoxyoligonucleotides by the phosphoramidite method [J ] . Methods in Enzymology , 1987 , 154 : 287 - 313 .

CARUTHERS M H . Gene synthesis machines: DNA chemistry and its uses [J ] . Science , 1985 , 230 ( 4723 ): 281 - 285 .

LIETARD J , SCHAUDY E , HOLZ K , et al . High-density DNA and RNA microarrays-photolithographic synthesis, hybridization and preparation of large nucleic acid libraries [J ] . Journal of Visualized Experiments , 2019 ( 150 ): 1 - 13 .

MATTEUCCI M D , CARUTHERS M H . Synthesis of deoxyoligonucleotides on a polymer support [J ] . Journal of the American Chemical Society , 1981 , 103 ( 11 ): 3185 - 3191 .

EROSHENKO N , KOSURI S , MARBLESTONE A H , et al . Gene assembly from chip-synthesized oligonucleotides [J ] . Current Protocols Chemical Biology , 2012 , 4 ( 1 ): 1 - 24 .

汤建新 , 陈洪 , 何农跃 . 活版印刷技术原位合成DNA微阵列 [J ] . 中国科学(B辑化学) , 2006 , 36 ( 6 ): 493 - 500 .

TANG J X , CHEN H , HE N Y . In situ synthesis of DNA microarrays by letterpress printing [J ] . SCIENCE IN CHINA Ser. B Chemistry , 2006 , 36 ( 6 ): 493 - 500 .

MASKOS U , SOUTHERN E M . A novel method for the parallel analysis of multiple mutations in multiple samples [J ] . Nucleic Acids Research , 1993 , 21 ( 9 ): 2269 - 2270 .

SOUTHERN E M , MASKOS U , ELDER J K . Analyzing and comparing nucleic acid sequences by hybridization to arrays of oligonucleotides: evaluation using experimental models [J ] . Genomics , 1992 , 13 ( 4 ): 1008 - 1017 .

SOUTHERN E M , CASE-GREEN S C , EIDER J K , et al . Arrays of complementary oligonucleotides for analysing the hybridisation behaviour of nucleic acids [J ] . Nucleic Acids Research , 1994 , 22 ( 8 ): 1368 - 1373 .

GAO X , GULARI E , ZHOU X . In situ synthesis of oligonucleotide microarrays [J ] . Biopolymers , 2004 , 73 ( 5 ): 579 - 596 .

WANG C , TRAU D . A portable generic DNA bi oassay system based on in situ oligonucleotide synthesis and hybridization detection [J ] . Biosensors Bioelectronics , 2011 , 26 ( 5 ): 2436 - 2441 .

SUMMERER D , HEVRONI D , JAIN A , et al . A flexible and fully integrated system for amplification, detection and genotyping of genomic DNA targets based on microfluidic oligonucleotide arrays [J ] . Nature Biotechnology , 2010 , 27 ( 2 ): 149 - 155 .

KIM C , KAYSEN J , RICHMOND K , et al . Progress in gene assembly from a MAS-driven DNA microarray [J ] . Microelectronic Engineering , 2006 , 83 ( 4 /5/6/7/ 8 / 9 ): 1613 - 1616 .

RICHMOND K E , LI M H , RODESCH M J , et al . Amplification and assembly of chip-eluted DNA (AACED): a method for high-throughput gene synthesis [J ] . Nucleic Acids Research , 2004 , 32 ( 17 ): 5011 - 5018 .

ZHOU X C , CAI S Y , HONG A L , et al . Microfluidic PicoArray synthesis of oligodeoxynucleotides and simultaneous assembling of multiple DNA sequences [J ] . Nucleic Acids Research , 2004 , 32 ( 18 ): 5409 - 5417 .

FODOR S P , READ J L , PIRRUNG M C , et al . Light-directed, spatially addressable parallel chemical synthesis [J ] . Science , 1991 , 251 ( 4995 ): 767 - 773 .

LIPSHUTZ R J , FODOR S P A , GINGERAS T R , et al . High density synthetic oligonucleotide arrays [J ] . Nature Genetics , 1999 , 21 : 20 - 24 .

PEASE A C , SOLAS D , SULLIVAN E J , et al . Light-generated oligonucleotide arrays for rapid DNA sequence analysis [J ] . Proceedings of the National Academy of Sciences of the United States of America , 1994 , 91 ( 11 ): 5022 - 5026 .

FODOR S P , RAVA R P , HUANG X C , et al . Multiplexed biochemical assays with biological chips [J ] . Nature , 1993 , 364 ( 6437 ): 555 - 556 .

DALMA-WEISZHAUSZ D , WARRINGTON D , JANET T , et al . The affymetrix geneChip ® platform: an overview [J ] . Methods Enzymol , 2006 , 410 : 3 - 28 .

SINGH-GASSON S , GREEN R D , YUE Y , et al . Maskless fabrication of light-directed oligonucleotide microarrays using a digital micromirror array [J ] . Nature Biotechnology , 1999 , 17 ( 10 ): 974 - 978 .

CERRINA F , BLATTNER F , HUANG W , et al . Biological lithography: development of a maskless microarray synthesizer for DNA chips [J ] . Microelectronic Engineering , 2002 , 61/62 : 33 - 40 .

NUWAYSIR E F , HUANG W , ALBERT T J , et al . Gene expression analysis using oligonucleotide arrays produced by maskless photolithography [J ] . Genome Research , 2002 , 12 ( 11 ): 1749 - 1755 .

AGBAVWE C , KIM C , HONG D , et al . Efficiency, error and yield in light-directed maskless synthesis of DNA microarrays [J ] . Journal of Nanobiotechnology , 2011 , 9 : 57 .

SACK M , KRETSCHY N , ROHM B , et al . Simultaneous light-directed synthesis of mirror-image microarrays in a photochemical reaction cell with flare suppression [J ] . Analytical Chemistry , 2013 , 85 ( 18 ): 8513 - 8517 .

SACK M , HÖLZ K , HOLIK A K , et al . Express photolithographic DNA microarray synthesis with optimized chemistry and high-efficiency photolabile groups [J ] . Journal of Nanobiotechnology , 2016 , 14 ( 1 ): 1 - 13 .

KRETSCHY N , HOLIK A K , SOMOZA V , et al . Next-generation o-nitrobenzyl photolabile groups for light-directed chemistry and microarray synthesis [J ] . Angewandte Chemie International Edition , 2015 , 54 ( 29 ): 8555 - 8559 .

LACKEY J G , MITRA D , SOMOZA M M , et al . Acetal levulinyl ester (ALE) groups for 2 ' -hydroxyl protection of ribonucleosides in the synthesis of oligoribonucleotides on glass and microarrays [J ] . Journal of the American Chemical Society , 2009 , 131 ( 24 ): 8496 - 8502 .

MCGALL G , LABADIE J , BROCK P , et al . Light-directed synthesis of high-density oligonucleotide arrays using semiconductor photoresists [J ] . Proceedings of the National Academy of Sciences of the United States of America , 1996 , 93 ( 24 ): 13555 - 13560 .

KIM C , LI M , RODESCH M , et al . Biological lithography: improvements in DNA synthesis methods [J ] . Journal of Vacuum Science & Technology B , 2004 , 22 ( 6 ): 3163 - 3167 .

PAWLOSKI A R , MCGALL G , KUIMELIS R G , et al . Photolithographic synthesis of high-density DNA probe arrays: challenges and opportunities [J ] . Journal of Vacuum Science & Technology B , 2007 , 25 ( 6 ): 2537 - 2546 .

PEASE A C , SOLAS D , SULLIVAN E J , et al . Light-generated oligonucleotide arrays for rapid DNA-sequence analysis [J ] . Proceedings of the National Academy of Sciences of the United States of America , 1994 , 91 ( 11 ): 5022 - 5026 .

ALBERT T J , NORTON J , OTT M , et al . Light-directed 5 ' →3 ' synthesis of complex oligonucleotide microarrays [J ] . Nucleic Acids Research , 2003 , 31 ( 7 ): e35 .

GAO X L , LEPROUST E , ZHANG H , et al . A flexible light-directed DNA chip synthesis gated by deprotection using solution photogenerated acids [J ] . Nucleic Acids Research , 2001 , 29 ( 22 ): 4744 - 4750 .

SRIVANNAVIT O , GULARI M , HUA Z , et al . Microfluidic reactor array device for massively parallel in situ synthesis of oligonucleotides [J ] . Sensors and Actuators B: Chemical , 2009 , 140 ( 2 ): 473 - 481 .

LEPROUST E , PELLOIS J P , YU P L , et al . Digital light-directed synthesis. A microarray platform that permits rapid reaction optimization on a combinatorial basis [J ] . Journal of Combinatorial Chemistry , 2000 , 2 ( 4 ): 349 - 354 .

KOMOLPIS K , SRIVANNAVIT O , GULARI E . Light-directed simultaneous synthesis of oligopeptides on microarray substrate using a photogenerated acid [J ] . Biotechnology Progress , 2002 , 18 ( 3 ): 641 - 646 .

HUGHES T R , MAO M , JONES A R , et al . Expression profiling using microarrays fabricated by an ink-jet oligonucleotide synthesizer [J ] . Nature Biotechnology , 2001 , 19 ( 4 ): 342 - 347 .

YANG Y H , DUDOIT S , LUU P , et al . Normalization for cDNA microarray data: a robust composite method addressing single and multiple slide systematic variation [J ] . Nucleic Acids Research , 2002 , 30 ( 4 ): e15 .

BAUM M , BIELAU S , RITTNER N , et al . Validation of a novel, fully integrated and flexible microarray benchtop facility for gene expression profiling [J ] . Nucleic Acids Research , 2003 , 31 ( 23 ): e151 .

HUGHES T R , MAO M , JONES A R , et al . Expression profiling using microarrays fabricated by an ink-jet oligonucleotide synthesizer [J ] . Nature Biotechnology , 2001 , 19 ( 4 ): 342 - 347 .

MRKSICH M , WHITESIDES G M . Patterning self-assembled monolayers using microcontact printin-a new technology for biosensors [J ] . Trends in Biotechnology , 1995 , 13 ( 6 ): 228 - 235 .

MRKSICH M , CHEN C S , XIA Y , et al . Controlling cell attachment on contoured surfaces with self-assembled monolayers of alkanethiolates on gold [J ] . Proceedings of the National Academy of Sciences of the United States of America , 1996 , 93 ( 20 ): 10775 - 10778 .

JAMES C D , DAVIS R C , KAM L , et al . Patterned protein layers on solid substrates by thin stamp microcontact printing [J ] . Langmuir , 1998 , 14 ( 4 ): 741 - 744 .

MRKSICH M , DIKE L E , TIEN J , et al . Using microcontact printing to pattern the attachment of mammalian cells to self-assembled monolayers of alkanethiolates on transparent films of gold and silver [J ] . Experimental Cell Research , 1997 , 235 ( 2 ): 305 - 313 .

YAN L , ZHAO X M , WHITESIDES G M . Patterning a preformed, reactive SAM using microcontact printing [J ] . Journal of the American Chemical Society , 1998 , 120 ( 24 ): 6179 - 6180 .

YAN L , HUCK W T S , ZHAO X M , et al . Patterning thin films of poly(ethylene imine) on a reactive SAM using microcontact printing [J ] . Langmuir , 1999 , 15 ( 4 ): 1208 - 1214 .

LAHIRI J , OSTUNI E , WHITESIDES G M . Patterning ligands on reactive SAMs by microcontact printing [J ] . Langmuir , 1999 , 15 ( 6 ): 2055 - 2060 .

陆祖宏 , 何农跃 , 赵雨杰 , 等 . 制备化合物微阵列芯片的方法及由该方法制备的化合物微阵列芯片 : CN1274758 [P ] . 2004-07-28 .

LU Z H , HE N Y , ZHAO Y J , et al . Method for preparing compound microarray chip and compound microarray chip prepared by the method : CN1274758 [P ] . 2004-07-28 .

肖鹏峰 , 何农跃 , 贺全国 , 等 . 分子印章法DNA芯片的合成 [J ] . 中国科学(B辑化学) , 2001 , 31 ( 5 ): 399 - 404 .

XIAO P F , HE N Y , HE Q G , et al . Synthesis of DNA chip by molecular seal method [J ] . SCIENCE IN CHINA Ser. B Chemistry , 2001 , 31 ( 5 ): 399 - 404 .

肖鹏峰 , 陆祖宏 , 刘正春 , 等 . 分子印章法DNA芯片合成(Ⅲ)——反应条件的优化 [J ] . 科学通报 , 2002 , 47 ( 3 ): 189 - 192 .

XIAO P F , LU Z H , LIU Z C , et al . DNA chip synthesis by molecular seal method (Ⅲ)-optimization of reaction conditions [J ] . Chinese Science Bulletin , 2002 , 47 ( 3 ): 189 - 192 .

肖鹏峰 , 何农跃 , 朱纪军 , 等 . 分子印章法DNA芯片的合成(Ⅰ) [J ] . 东南大学学报(自然科学版) , 2001 , 31 ( 2 ): 756 - 762 .

XIAO P F , HE N Y , ZHU J J , et al . DNA microarray synthesis using PDMS molecular stamps (Ⅰ): the fixation and shrinkage of PDMS molecular stamps [J ] . Journal of Southeast University (Natural Science Edition) , 2001 , 31 ( 2 ): 756 - 762 .

XIA Y , WHITESIDES G M . Soft lithography [J ] . Annual Review of Materials Science , 1998 , 28 ( 1 ): 153 - 184 .

孙啸 , 王晔 , 赵雨杰 , 等 . 一种高密度基因芯片设计的新方法 [J ] . 电子学报 , 2001 , 29 ( 3 ): 293 - 296 .

SUN X , WANG Y , ZHAO Y J , et al . A new method of high-density gene chip design [J ] . Acta Electronica Sinica , 2001 , 29 ( 3 ): 293 - 296 .

王轶文 , 徐吉庆 , 陈扬 , 等 . 电解致酸脱保护DNA在片合成研究 [J ] . 东南大学学报(自然科学版) , 2002 , 32 ( 1 ): 77 - 80 .

WANG Y W , XU J Q , CHEN Y , et al . Study on on-chip synthesis of electrolytic acid-deprotected DNA [J ] . Journal of Southeast University (Natural Science Edition) , 2002 , 32 ( 1 ): 77 - 80 .

EGELAND R D , SOUTHERN E M . Electrochemically directed synthesis of oligonucleotides for DNA microarray fabrication [J ] . Nucleic Acids Research , 2005 , 33 ( 14 ): e125 .

MAURER K , YAZVENKO N , WILMOTH J , et al . Use of a multiplexed CMOS microarray to optimize and compare oligonucleotide binding to DNA probes synthesized or immobilized on individual electrodes [J ] . Sensors , 2010 , 10 ( 8 ): 7371 - 7385 .

GHINDILIS A L , SMITH M W , SCHWARZKOPF K R , et al . CombiMatrix oligonucleotide arrays: genotyping and gene expression assays employing electrochemical detection [J ] . Biosensors & Bioelectronics , 2007 , 22 ( 9/10 ): 1853 - 1860 .

COOPER J , YAZVENKO N , PEYVAN K , et al . Targeted deposition of antibodies on a multiplex CMOS microarray and optimization of a sensitive immunoassay using electrochemical detection [J ] . PLoS One , 2010 , 5 ( 3 ): e9781 .

MAURER K , COOPER J , CARABALLO M , et al . Electrochemically generated acid and its containment to 100 micron reaction areas for the production of DNA microarrays [J ] . PLoS One , 2006 , 1 : e34 .

CHOW B Y , EMIG C J , JACOBSON J M . Photoelectrochemical synthesis of DNA microarrays [J ] . Proceedings of the National Academy of Sciences of the United States of America , 2009 , 106 ( 36 ): 15219 - 15224 .

CHOI Y , RYU T , LEE A C , et al . High information capacity DNA-based data storage with augmented encoding characters using degenerate bases [J ] . Scientific Reports , 2019 , 9 ( 1 ): 6582 .

GUO Y , DONG J , ZHOU T , et al . YeastFab: the design and construction of standard biological parts for metabolic engineering in Saccharomyces cerevisiae [J ] . Nucleic Acids Research , 2015 , 43 ( 13 ): e88 .

QIN Y R , TAN C , LIN J W , et al . EcoExpress—highly efficient construction and expression of multicomponent protein complexes in escherichia coli [J ] . ACS Synthetic Biology , 2016 , 5 ( 11 ): 1239 - 1246 .

PALLUK S , ARLOW D H , ROND T D , et al . De novo DNA synthesis using polymerase-nucleotide conjugates [J ] . Nature Biotechnology , 2018 , 36 ( 7 ): 645 - 650 .

JENSEN M A , DAVIS R W . Template-independent enzymatic oligonucleotide synthesis (TiEOS): its history, prospects, and challenges [J ] . Biochemistry , 2018 , 57 ( 12 ): 1821 - 1832 .

SINDELAR L E , JAKLEVIC J M . High-throughput DNA synthesis in a multichannel format [J ] . Nucleic Acids Research , 1995 , 23 ( 6 ): 982 - 987 .

LASHKARI D A , HUNICKESMITH S P , NORGREN R M , et al . An automated multiplex oligonucleotide synthesizer-development of high-throughput, low-cost DNA-synthesis [J ] . Proceedings of the National Academy of Sciences of the United States of America , 1995 , 92 ( 17 ): 7912 - 7915 .

CHENG J Y , CHEN H H , KAO Y S , et al . High throughput parallel synthesis of oligonucleotides with 1536 channel synthesizer [J ] . Nucleic Acids Research , 2002 , 30 ( 18 ): e93 .

CHENG J Y , CHEN H Y . Microfluidic ARray Synthesizer (MArS) for rapid preparation and hybridization of custom DNA microarray [J ] . Biotechnology and Bioengineering , 2009 , 104 ( 2 ): 400 - 407 .

浏览量

下载量

CSCD

文章被引用时，请邮件提醒。

提交

工具集

关联资源

DNA存储的关键技术：编码、纠错、随机访问与安全性

DNA信息存储：生命系统与信息系统的桥梁

DNA合成、组装与纠错技术研究进展