合成生物学在蛋白质功能材料领域的研究进展

王盼; 朱晨辉; 赵婧; 范代娣

doi:10.12211/2096-8280.2020-061

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合成生物学在蛋白质功能材料领域的研究进展

特约评述 | 更新时间：2025-03-20

- 合成生物学在蛋白质功能材料领域的研究进展
- Research progress of synthetic biology in the field of protein functional materials
- 合成生物学 2021年2卷第1期页码：46-58
- 作者机构：
  
  1.西北大学化工学院，陕西省可降解生物医用材料重点实验室，陕西　西安　710069
  2.西北大学化工学院，陕西省生物材料与发酵工程技术研究中心，陕西　西安　710069
- 作者简介：
  
  [ "王盼（1989-），女，博士，讲师，主要研究方向为微生物酶学。E-mail：panwanghgxy@nwu.edu.cn" ]
  [ "范代娣（1965-），女，博士，教授,主要研究方向为可降解生物材料、预防医学和营养医学。E-mail：fandaidi@nwu.edu.cn" ]
- 基金信息：
  
  国家重点研发计划(2019YFA0905200);陕西省教育厅专项科研计划(20JK0938)
- DOI：10.12211/2096-8280.2020-061
  中图分类号： Q819
- 收稿：2020-04-28，
  
  修回：2020-09-17，
  
  纸质出版：2021-02-28
- 稿件说明：
移动端阅览
王盼，朱晨辉，赵婧，范代娣. 合成生物学在蛋白质功能材料领域的研究进展[J]. 合成生物学, 2021, 2(1): 46-58

WANG Pan, ZHU Chenhui, ZHAO Jing, FAN Daidi. Research progress of synthetic biology in the field of protein functional materials[J]. Synthetic Biology Journal, 2021, 2(1): 46-58
王盼，朱晨辉，赵婧，范代娣. 合成生物学在蛋白质功能材料领域的研究进展[J]. 合成生物学, 2021, 2(1): 46-58 DOI： 10.12211/2096-8280.2020-061.

WANG Pan, ZHU Chenhui, ZHAO Jing, FAN Daidi. Research progress of synthetic biology in the field of protein functional materials[J]. Synthetic Biology Journal, 2021, 2(1): 46-58 DOI： 10.12211/2096-8280.2020-061.

摘要

蛋白质功能材料具有良好的生物相容性、可降解性和多功能性，在医药、军事和纺织等领域应用价值巨大。然而，材料蛋白具有分子量大、高频氨基酸多和翻译后修饰特殊等独特性，导致其在人工细胞合成中存在表达率低、各功能元件与底盘细胞适配性差、结构及功效不稳定等瓶颈，严重限制了这些蛋白的高效生产与应用。掀起第三次生物技术革命的合成生物学技术，以“基因调控，工程设计”为核心，可用于产品的精准化设计与高效合成，实现人类对可持续发展工业模式的期待。本文介绍了蛋白质功能材料的应用及发展，以蛋白高效合成及功能需求为导向，从蛋白分子的定向设计、细胞工厂构建与适配调控以及蛋白材料加工应用等方面阐述蛋白质功能材料的合成策略，以实现其功能定向强化及工业化生产。目前以合成生物学的科学理念为指导，对生命体进行不断改造与优化，以实现细胞工厂定向合成蛋白，结合理性设计的材料模块，赋予蛋白质在生产过程的智能可控以及性能的更新升级是研究的热点。展望未来，讨论了蛋白质功能材料在面向产业应用过程中需要克服的挑战，为性能优异的蛋白质功能材料广泛应用提供可能。

Abstract

Protein functional materials have good biocompatibility

degradability and versatility

so they have great application values in the fields of medicine

military and textile. However

due to their unique characteristics of high molecular weight

high-frequency amino acids and special post-translation modifications

there are bottlenecks in its low expression rate in artificial cell synthesis

poor compatibility between functional elements and chassis cells

and unstable structure and efficacy

which seriously limit the efficient production and application of these proteins. Synthetic biology

as the third biotechnology revolution

has the advantages of renewable resources

low pollution

easy control and directional design of biological macromolecules. With the gradual excavation and analysis of the functional principles and novel design concepts of protein functional materials

and the development of alternative materials with excellent performance under specific conditions

it has brought revolutionary changes to human social life. It has been reported that such protein functional materials have great application value in cancer diagnosis and treatment

regenerative medicine

gene delivery system

data storage and so on. Although synthetic biology has broadened the range of potential applications of protein functional materials

there are still some limitations. This requires us to carry out research from two different perspectives

biology and materials science. On the one hand

we should establish a common technology platform to form a complete upper

middle and downstream research system. On the other hand

we should establish targeted research based on the characteristics of different protein materials to produce materials with better performance. This article introduces the application and development of protein functional materials. Taking the efficient protein synthesis and functional requirements as the guide

the synthesis strategy of protein functional materials are explained from the aspects of protein molecule orientation design

cell factory construction and adaptation control

and protein material processing applications

in order to realize their functional directional enhancement and industrial production. The limitations of synthetic biology oriented protein functional materials in biology and materials science are prospected

which lay a foundation for the wide application of protein functional materials with excellent properties.

关键词

Keywords

references

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