合成生物制造进展

张媛媛; 曾艳; 王钦宏

doi:10.12211/2096-8280.2020-052

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合成生物制造进展

特约评述 | 更新时间：2025-03-20

- 合成生物制造进展
  封面论文
- Advances in synthetic biomanufacturing
- 合成生物学 2021年2卷第2期页码：145-160
- 作者机构：
  
  1.中国科学院天津工业生物技术研究所，中国科学院系统微生物工程重点实验室，天津 300308
  2.中国科学院科技促进发展局，北京 100864
- 作者简介：
  
  [ "张媛媛（1985—），女，硕士，助理研究员。研究方向为进化与代谢工程。E-mail：zhang_yy@tib.cas.cn" ]
  [ "王钦宏（1974—），男，博士，研究员。研究方向为化学品代谢途径构建，发展基因组水平编辑和进化策略以及液滴微流控高通量筛选系统，进行生产重要化学品的细胞工厂优化改造，获得实用高性能微生物细胞工厂。E-mail：wang_qh@tib.cas.cn" ]
- 基金信息：
  
  国家新药创制重大专项(2018ZX09711001-006-003);中国科学院科技服务网络计划（STS计划）(KFJ-STS-ZDTP-065)
- DOI：10.12211/2096-8280.2020-052
  中图分类号： Q81
- 收稿：2020-06-07，
  
  修回：2021-02-04，
  
  纸质出版：2021-04-30
- 稿件说明：
移动端阅览
张媛媛, 曾艳, 王钦宏. 合成生物制造进展[J]. 合成生物学, 2021, 2(2): 145-160

ZHANG Yuanyuan, ZENG Yan, WANG Qinhong. Advances in synthetic biomanufacturing[J]. Synthetic Biology Journal, 2021, 2(2): 145-160
张媛媛, 曾艳, 王钦宏. 合成生物制造进展[J]. 合成生物学, 2021, 2(2): 145-160 DOI： 10.12211/2096-8280.2020-052.

ZHANG Yuanyuan, ZENG Yan, WANG Qinhong. Advances in synthetic biomanufacturing[J]. Synthetic Biology Journal, 2021, 2(2): 145-160 DOI： 10.12211/2096-8280.2020-052.

摘要

合成生物制造是以合成生物为工具进行物质加工与合成的生产方式，有望彻底变革未来医药、化工、食品、能源、材料、农业等传统模式，触发新的产业变革，引领新的产业模式和经济形态，重塑碳基物质文明。合成生物制造具有清洁、高效、可再生等特点，能够减少工业经济对生态环境的影响。本文综述了近年来合成生物制造在大宗发酵产品、精细与医药化学品、可再生化学品与聚合材料、天然产物、未来农产品以及一碳原料利用方面的重要进展，对各领域代表性重大产品的技术进展及产业应用状况与潜力进行了探讨。未来，随着合成生物学发展，以及与人工智能、大数据等新技术的融合，通过合成生物制造可以获得更多的生物基产品，促进生物经济形成，更好地服务于人类社会的可持续发展。

Abstract

Synthetic biomanufacturing is a paradigm for material processing and synthesis via synthetic biology. It is expected to completely transform the traditional production mode for medicines

chemicals

food

energy

materials and agriculture in the future

trigger new industrial revolution

lead to new economic growth and reshape carbon-based civilization. In particular

the COVID-19 global spread that has accelerated the reshaping process of the world's economic and social development. In the foreseeable future

human life and production patterns will undergo profound changes in medicines and healthcare

food and agriculture

energy and materials

etc.

during which demands for new technologies will promote evolution in the field of biotechnology

and the biomanufacturing industry in the post COVID-19 era is facing unprecedented opportunities for revitalization and new challenges. According to the analysis of the research report from the Ministry of Economy

Trade and Industry of Japan

engineered biological cells and their combination with information

artificial intelligence technologies will become the main driving force for the "post-fourth industrial revolution".Synthetic biomanufacturing has the characteristics of cleanliness

efficiency and renewableness that can reduce the impact of industrial economy on the ecological environment. Here

in this review

we summarize the progress of synthetic biomanufacturing with respect to bulk fermentation products

fine and pharmaceutical chemicals

renewable chemic

als and bio-based polymeric materials

natural products

foods and the utilization of C

raw materials. The technological progress

status and potential of industrial applications of many important bio-based products

via

synthetic biomanufacturing are analyzed and discussed. The development of synthetic biomanufacturing shows great potentials for building up the ecological route of industrial economy and addressing current issues of economic sustainability in terms of limited substrate

high cost

and poor viability

and to form whole new industry chain with sustainable growth. In the future

with the development of synthetic biology

and the integration of new technologies such as artificial intelligence and big data

more and more bio-based products can be produced via synthetic biomanufacturing. The formation of bioeconomy can be promoted

and the sustainable development of human society will be better served.

关键词

Keywords

references

CAMERON D E , BASHOR C J , COLLINS J J . A brief history of synthetic biology [J ] . Nature Reviews Microbiology , 2014 , 12 ( 5 ): 381 - 390 .

WAY J C , COLLINS J J , KEASLING J D , et al . Integrating biological redesign: where synthetic biology came from and where it needs to go [J ] . Cell , 2014 , 157 ( 1 ): 151 - 161 .

ENDY D . Foundations for engineering biology [J ] . Nature , 2005 , 438 ( 7067 ): 449 - 453 .

CHURCH G M , ELOWITZ M B , SMOLKE C D , et al . Realizing the potential of synthetic biology [J ] . Nature Reviews Molecular Cell Biology , 2014 , 15 ( 4 ): 289 - 294 .

CLOMBURG J M , CRUMBLEY A M , GONZALEZ R . Industrial Biomanufacturing: The future of chemical production [J ] . Science , 2017 , 355 : 6320 .

ZHANG Y P , SUN J , MA Y . Biomanufacturing: history and perspective [J ] . Journal of Industrial Microbiology & Biotechnology , 2017 , 44 ( 4/5 ): 773 - 784 .

曾艳 , 赵心刚 , 周桔 . 合成生物学工业应用的现状和展望 [J ] . 中国科学院院刊 , 2018 , 33 ( 11 ): 1211 - 1217 .

ZENG Y , ZHAO X G , ZHOU J . Current situations and perspectives of industrial applications of synthetic biology [J ] . Bulletin of the Chinese Academy of Sciences , 2018 , 33 ( 11 ): 1211 - 1217 .

卢涛 , 石维忱 . 我国生物发酵产业现状分析与发展策略 [J ] . 生物产业技术 , 2019 ( 2 ): 5 - 8 .

LU T , SHI W C . Current situation analysis and development strategy of biological fermentation industry in China [J ] . Biotechnology & Business , 2019 ( 2 ): 5 - 8 .

LIU J J , LI J H , SHIN H D , et al . Protein and metabolic engineering for the production of organic acids [J ] . Bioresource Technology , 2017 , 239 : 412 - 421 .

CHEN Y , NIELSEN J . Biobased organic acids production by metabolically engineered microorganisms [J ] . Current Opinion In Biotechnology , 2016 , 37 : 165 - 172 .

TONG Z Y , ZHENG X M , TONG Y , et al . Systems metabolic engineering for citric acid production by Aspergillus niger in the post-genomic era [J ] . Microbial Cell Factories , 2019 , 18 : 28 .

HU W , LI W J , YANG H Q , et al . Current strategies and future prospects for enhancing microbial production of citric acid [J ] . Applied Microbiology and Biotechnology , 2019 , 103 ( 1 ): 201 - 209 .

STEIGER M G , RASSINGER A , MATTANOVICH D , et al . Engineering of the citrate exporter protein enables high citric acid production in Aspergillus niger [J ] . Metabolic Engineering , 2019 , 52 : 224 - 231 .

LIU J J , LI J H , SHIN H D , et al . Biological production of L-malate: recent advances and future prospects [J ] . World Journal of Microbiology and Biotechnology , 2019 , 34 ( 1 ): 6 .

LI J G , LIN L C , SUN T , et al . Direct production of commodity chemicals from lignocellulose using Myceliophthora thermophila [J ] . Metabolic Engineering , 2019 , 64 : 416 - 426 .

ZHAO M L , LU X Y , ZONG H , et al . Itaconic acid production in microorganisms [J ] . Biotechnology Letters , 2018 , 40 ( 3 ): 455 - 464 .

KARAFFA L , DIAZ R , PAPP B , et al . A deﬁciency of manganese ions in the presence of high sugar concentrations is the critical parameter for achieving high yields of itaconic acid by Aspergillus terreus [J ] . Applied Microbiology and Biotechnology , 2015 , 99 ( 19 ): 7937 – 7944 .

WENDISCH V F . Metabolic engineering advances and prospects for amino acid production [J ] . Metabolic Engineering , 2020 , 58 : 17 - 34 .

HIRASAWA T , SHIMIZU H . Recent advances in amino acid production by microbial cells [J ] . Current Opinion In Biotechnology , 2016 , 42 : 133 - 146 .

GENG F , CHEN Z , ZHENG P , et al . Exploring the allosteric mechanism of dihydrodipicolinate synthase by reverse engineering of the allosteric inhibitor binding sites and its application for lysine production [J ] . Applied Microbiology and Biotechnology , 2013 , 97 ( 5 ): 1963 - 71 .

WANG X W , LI Q G , SUN C M , et al . GREACE-assisted adaptive laboratory evolution in endpoint fermentation broth enhances lysine production by Escherichia coli [J ] . Microbial Cell Factories , 2019 , 18 ( 1 ): 106 .

HUANG J F , LIU Z Q , JIN L Q , et al . Metabolic engineering of Escherichia coli for microbial production of L-methionine [J ] . Biotechnology and Bioengineering , 2017 , 114 ( 4 ): 843 - 851 .

PARK S H , KIM H U , KIM T Y , et al . Metabolic engineering of Corynebacterium glutamicum for L-arginine production [J ] . Nature Communications , 2014 , 5 : 4618 .

OZCENGIZ G , DEMAIN A L . Recent advances in the biosynthesis of penicillins, cephalosporins and clavams and its regulation [J ] . Biotechnology Advances , 2013 , 31 ( 2 ): 287 - 311 .

FAN K Q , LIN B X , TAO Y , et al . Engineering deacetoxycephalosporin C synthase as a catalyst for the bioconversion of penicillins [J ] . Journal of Industrial Microbiology & Biotechnology , 2017 , 44 ( 4/5 ): 705 - 710 .

PALAZZOTTO E , TONG Y J , LEE S Y , et al . Synthetic biology and metabolic engineering of actinomycetes for natural product discovery [J ] . Biotechnology Advances , 2019 , 37 ( 6 ): 107366 .

DHAKAL D , SOHNG J K , PANDEY R P . Engineering actinomycetes for biosynthesis of macrolactone polyketides [J ] . Microbial Cell Factories , 2019 , 18 ( 1 ): 137 .

WEBER T , CHARUSANTI P , MUSIOL-KROLL E M , et al . Metabolic engineering of antibiotic factories: new tools for antibiotic production in actinomycetes [J ] . Trends in Biotechnology , 2015 , 33 ( 1 ): 15 - 26 .

TEIJARO C N , ADHIKARI A , SHEN B . Challenges and opportunities for natural product discovery, production, and engineering in native producers versus heterologous hosts [J ] . Journal of Industrial Microbiology & Biotechnology , 2019 , 46 ( 3/4 ): 433 - 444 .

LU Z L , ZHANG X T , DAI J L , et al . Engineering of leucine-responsive regulatory protein improves spiramycin and bitespiramycin biosynthesis [J ] . Microbial Cell Factories , 2019 , 18 : 38 .

YUAN P H , CUI S X , LIU Y F , et al . Metabolic engineering for the production of fat-soluble vitamins: advances and perspectives [J ] . Applied Microbiology and Biotechnology , 2020 , 104 ( 3 ): 935 - 951 .

ACEVEDO-ROCHA C G , GRONENBERG L S , MACK M , et al . Microbial cell factories for the sustainable manufacturing of B vitamins [J ] . Current Opinion in Biotechnology , 2019 , 56 : 18 - 29 .

ZHOU J W , DU G C , CHEN J . Metabolic engineering of microorganisms for vitamin C production [J ] . Sub-cellular biochemistry , 2012 , 64 : 241 - 259 .

PEI X L , YANG Z F , WANG A M , et al . Identification and functional analysis of the activator gene involved in the biosynthesis of Co-type nitrile hydratase from Aurantimonas manganoxydans [J ] . Journal of Biotechnology , 2017 , 251 : 38 - 46 .

FANG H , LI D , KANG J , et al . Metabolic engineering of Escherichia coli for de novo biosynthesis of vitamin B 12 [J ] . Nature Communications , 2018 , 9 : 4917 .

AGUILAR A , TWARDOWSKI T , WOHLGEMUTH R . Bioeconomy for sustainable development [J ] . Biotechnology Journal , 2019 , 14 ( 8 ): e1800638 .

BIZ A , PROULX S , XU Z , et al . Systems biology based metabolic engineering for non-natural chemicals [J ] . Biotechnology Advances , 2019 , 37 ( 6 ): 107379 .

BURK M J , DIEN , S V . Biotechnology for chemical production: challenges and opportunities [J ] . Trends in Biotechnology , 2016 , 34 ( 3 ): 187 - 190 .

ZHANG Y , LIU D H , CHEN Z . Production of C 2 -C 4 diols from renewable bioresources: new metabolic pathways and metabolic engineering strategies [J ] . Biotechnology for Biofuels , 2017 , 10 : 299 .

LEE S Y , KIM H U . Systems strategies for developing industrial microbial strains [J ] . Nature Biotechnology , 2015 , 33 ( 10 ), 1061 - 1072 .

NIELSEN J , KEASLING J D . Engineering Cellular Metabolism [J ] . Cell , 2016 , 164 ( 6 ): 1185 - 1197 .

ZHU X N , TAN Z G , XU H T , et al . Metabolic evolution of two reducing equivalent-conserving pathways for high-yield succinate production in Escherichia coli [J ] . Metabolic Engineering , 2014 , 24 : 87 - 96 .

XIAO M Y , ZHU X N , FAN F Y , et al . Osmotolerance in Escherichia coli is improved by activation of copper efflux genes or supplementation with sulfur containing amino acids [J ] . Applied and Environmental Microbiology , 2017 , 83 ( 7 ): e03050 .

KOU F Y , ZHAO J , LIU , J , et al . Enhancement of the thermal and alkaline pH stability of Escherichia coli lysine decarboxylase for efficient cadaverine production [J ] . Biotechnology Letters , 2018 , 40 ( 4 ): 719 - 727 .

YIM H , HASELBECK R , NIU W , et al . Metabolic engineering of Escherichia coli for direct production of 1,4-butanediol [J ] . Nature Chemical Biology , 2011 , 7 ( 7 ): 445 - 452 .

BURGARD A , BURK M J , OSTERHOUT R , et al . Development of a commercial scale process for production of 1,4-butanediol from sugar [J ] . Current Opinion In Biotechnology , 2016 , 42 : 118 - 125 .

YAN Q , PFLEGER B F . Revisiting metabolic engineering strategies for microbial synthesis of oleochemicals [J ] . Metabolic Engineering , 2020 , 58 : 35 - 46 .

CHOI S Y , RHIE M N , KIM H T , et al . Metabolic engineering for the synthesis of polyesters: a 100-year journey from polyhydroxyalkanoates to non-natural microbial polyesters [J ] . Metabolic Engineering , 2020 , 58 : 47 - 81 .

LEE Y , CHO I J , CHOI S Y , et al . Systems metabolic engineering strategies for non-natural microbial polyester production [J ] . Biotechnology Journal , 2019 , 14 ( 9 ): e1800426 .

JUTURU V , WU J C . Microbial production of lactic acid: the latest development [J ] . Critical Reviews In Biotechnology , 2016 , 36 ( 6 ): 967 - 977 .

JUNG Y K , KIM T Y , PARK S J , et al . Metabolic engineering of Escherichia coli for the production of polylactic acid and its copolymers [J ] . Biotechnology And Bioengineering , 2010 , 105 ( 1 ): 161 - 171 .

YANG T H , KIM T W , KANG H O , et al . Biosynthesis of polylactic acid and its copolymers using evolved propionate CoA transferase and PHA synthase [J ] . Biotechnology and Bioengineering , 2010 , 105 ( 1 ): 150 - 160 .

CHOI S Y , PARK S J , KIM W J , et al . One-step fermentative production of poly(lactate-co-glycolate) from carbohydrates in Escherichia coli [J ] . Nature Biotechnology , 2016 , 34 ( 4 ): 435 - 40 .

MENG D C , SHI Z Y , WU L P , et al . Production and characterization of poly(3-hydroxypropionate- co -4-hydroxybutyrate) with fully controllable structures by recombinant Escherichia coli containing an engineered pathway [J ] . Metabolic Engineering , 2012 , 14 ( 4 ): 317 - 324 .

ZHUANG Q Q , WANG Q , LIANG Q F , et al . Synthesis of polyhydroxyalkanoates from glucose that contain medium-chain-length monomers via the reversed fatty acid beta -oxidation cycle in Escherichia coli [J ] . Metabolic Engineering , 2014 , 24 : 78 - 86 .

YUE H T , LING C , YANG T , et al . A seawater-based open and continuous process for polyhydroxyalkanoates production by recombinant Halomonas campaniensis LS21 grown in mixed substra tes [J ] . Biotechnol Biofuels , 2014 , 7 : 108 .

WANG Y , YIN J , CHEN G Q . Polyhydroxyalkanoates, challenges and opportunities [J ] . Current Opinion In Biotechnology , 2014 , 30 : 59 - 65 .

WELLS A S , WONG J W , MICHELS P C , et al . Case studies illustrating a science and risk-based approach to ensuring drug quality when using enzymes in the manufacture of active pharmaceuticals ingredients for oral dosage form [J ] . Organic Process Research & Development , 2016 , 20 ( 3 ): 594 - 601 .

CURRIN A , SWAINSTON N , DAY P J , et al . Synthetic biology for the directed evolution of protein biocatalysts: navigating sequence space intelligently [J ] . Chemical Society Reviews , 2015 , 44 ( 5 ): 1172 - 1239 .

YOU C , SHI T , LI Y J , et al . An in vitro synthetic biology platform for the industrial biomanufacturing of myo-inositol from starch [J ] . Biotechnology and Bioengineering , 2017 , 114 ( 8 ): 1855 - 1864 .

TANG E J , SHEN X L , WANG J , et al . Synergetic utilization of glucose and glycerol for efficient myo-inositol biosynthesis [J ] . Biotechnology and Bioengineering , 2020 , 117 ( 4 ): 1247 - 1252 .

CAO M F , GAO M R , SUÁSTEGUI M , et al . Building microbial factories for the production of aromatic amino acid pathway derivatives: from commodity chemicals to plant-sourced natural products [J ] . Metabolic Engineering , 2020 , 58 : 94 - 132 .

王钦宏 , 陈五九 , 曹鹏 , 等 . 生产左旋多巴大肠杆菌重组菌株及其构建方法与应用 : CN201711003046.9 [P ] . 2017-10-24 .

WANG Q H , CHEN W J , CAO P , et al . Recombinant strain for producing levodopa E . coli as well as construction method and application thereof : CN201711003046.9 [P ] . 2017-10-24 .

蔡宇杰 , 刘金彬 , 李朝智 , 等 . 一种生产羟基酪醇的方法 : CN201811234787.2 [P ] . 2018-10-23 .

CAI Y J , LIU J B , LI C Z , et al . Method for producing hydroxytyrosol : CN201811234787.2 [P ] . 2018-10-23 .

BONTPART T , MARLIN T , VIALET S , et al . Two shikimate dehydrogenases, VvSDH3 and VvSDH4, are involved in gallic acid biosynthesis in grapevine [J ] . Journal of Experimental Botany , 2016 , 67 ( 11 ): 3537 - 3550 .

NODA S , SHIRAI T , OYAMA S , et al . Metabolic designofaplatform Escherichia coli strainproducingvarious chorismatederivatives [J ] . Metabolic Engineering , 2016 , 33 : 119 - 129 .

LIU Y F , XU Y R , DING D Q , et al . Genetic engineering of Escherichia coli to improve L-phenylalanine production [J ] . BMC Biotechnology , 2018 , 18 ( 1 ): 5 .

CHEN X R , WANG Z Y , GUO X N , et al . Regulation of general amino acid permeases Gap1p, GATAtranscription factors Gln3p and Gat1p on 2-phenylethanol biosynthesis via Ehrlich pathway [J ] . Journal of Biotechnology , 2017 , 242 : 83 - 91 .

MASUO S , KOBAYASHI Y , OINUMA K I , et al . Alternative fermentation pathway of cinnamic acid production via phenyllactic acid [J ] . Applied Microbiology and Biotechnology , 2016 , 100 ( 20 ): 8701 - 8709 .

CHEN Y Y , LIU Y F , DING D Q . Rational design and analysis of an Escherichia coli strain for high‑efficiency tryptophan production [J ] . Journal of Industrial Microbiology & Biotechnology , 2018 , 45 ( 5 ): 357 - 367 .

CHEN Z Y , SHEN X L , WANG J , et al . Establishing an artificial pathway for de novo biosynthesis of vanillyl alcohol in Escherichia coli [J ] . ACS Synthetic Biology , 2017 , 6 ( 9 ): 1784 - 1792 .

CHOI S-S , SEO S-Y , PARK S-O , et al . Cell factory design and culture process optimization for dehydroshikimate biosynthesis in Escherichia coli [J ] . Frontiers Bioengineering and Biotechnoogy , 2019 , 7 : 241 .

HUCCETOGULLARI D , LUO Z W , LEE S Y . Metabolic engineering of microorganisms for production of aromatic compounds [J ] . Microbial Cell Factories , 2019 , 18 ( 1 ): 41 .

LI L P , TU R , SONG G T , et al . Development of a synthetic 3-dehydroshikimate biosensor in Escherichia coli for metabolite monitoring and genetic screening [J ] . ACS Synthetic Biology , 2019 , 8 ( 2 ): 297 - 306 .

WU F L , CAO P , SONG G T , et al . Expanding the repertoire of aromatic chemicals by microbial production [J ] . Journal of Chemical Technology and Biotechnology , 2018 , 93 ( 10 ): 2804 - 2816 .

FERNÁNDEZ-CABEZÓN L , GALÁN B , GARCÍA J L . New insights on steroid biotechnology [J ] . Frontiers in Microbiology , 2018 , 9 : 958 .

LI Z , JIANG Y Y , GUENGERICH F P , et al . Engineering cytochrome P450 enzyme systems for biomedical and biotechnological applications [J ] . Journal of Biological Chemistry , 2020 , 295 ( 3 ): 833 - 849 .

DONOVA M V . Steroid bioconversions [J ] . Methods in Molecular Biology , 2017 , 1645 : 1 - 13 .

CHEN J , FAN F Y , QU G , et al . Identification of Absidia orchidis steroid 11 beta -hydroxylation system and its application in engineering Saccharomyces cerevisiae for one-step biotransformation to produce hydrocortisone [J ] . Metabolic Engineering , 2020 , 57 : 31 - 42 .

LU W , FENG J H , CHEN X , et al . Distinct regioselectivity of fungal P450 enzymes for steroidal hydroxylation [J ] . Applied and Environmental Microbiology , 2019 , 85 ( 18 ): e01182 .

LI X M , CHEN X , WANG Y , et al . New product identification in the sterol metabolism by an industrial strain Mycobacterium neoaurum NRRL B-3805 [J ] . Steroids , 2018 , 132 : 40 - 45 .

THOMFORD N E , SENTHEBANE D A , ROWE A , et al . Natural products for drug discovery in the 21st century: innovations for novel drug discovery [J ] . International Journal of Molecular Sciences , 2018 , 19 ( 6 ): 1578 .

CRAVENS A , PAYNE J , SMOLKE C D . Synthetic biology strategies for microbial biosynthesis of plant natural products [J ] . Nature Communications , 2019 , 10 ( 1 ): 2142 .

PADDON C J , WESTFALL P J , PITERA D J , et al . High-level semi-synthetic production of the potent antimalarial artemisinin [J ] . Nature , 2013 , 496 ( 7446 ): 528 - 532 .

AJIKUMAR P K , XIAO W H , TYO K E J , et al . Isoprenoid pathway optimization for Taxol precursor overproduction in Escherichia coli [J ] . Science , 2010 , 330 ( 6000 ): 70 - 74 .

GALANIE S , THODEY K , TRENCHARD I , et al . Complete biosynthesis of opioids in yeast [J ] . Science , 2015 , 349 ( 6252 ): 1095 - 1100 .

LI Y R , LI S L , THODEY K , et al . Complete biosynthesis of noscapine and halogenated alkaloids in yeast [J ] . Proceedings of the National Academy of Sciences of the United States of America , 2018 , 115 ( 17 ): E3922 - E3931 .

GEMPERLEIN K , DIETRICH D , KOHLSTEDT M , et al . Polyunsaturated fatty acid production by Yarrowia lipolytica employing designed myxobacterial PUFA synthases [J ] . Nature Communications , 2019 , 10 ( 1 ): 4055 .

LUO X Z , REITER M A , D'ESPAUX L , et al . Complete biosynthesis of cannabinoids and their unnatural analogues in yeast [J ] . Nature , 2019 , 567 ( 7746 ): 123 - 126 .

DAI Z B , LIU Y , ZHANG X N , et al . Metabolic engineering of Saccharomyces cerevisiae for production of ginsenosides [J ] . Metabolic Engineering , 2013 , 20 : 146 - 156 .

DAI Z B , WANG B B , LIU Y , et al . Producing aglycons of ginsenosides in bakers' yeast [J ] . Scientific Reports , 2014 , 4 : 3698 .

WANG P P , WEI W , YE W , et al . Synthesizing ginsenoside Rh 2 in Saccharomyces cerevisiae cell factory at high-efficiency [J ] . Cell Discovery , 2019 , 5 : 5 .

ZHAO J , LI Q Y , SUN T , et al . Engineering central metabolic modules of Escherichia coli for improving β -carotene production [J ] . Metabolic Engineering , 2013 , 17 : 42 - 50 .

SUN T , MIAO L T , LI Q Y , et al . Production of lycopene by metabolically-engineered Escherichia coli [J ] . Biotechnology Letters , 2014 , 36 ( 7 ): 1515 - 1522 .

GUO J , ZHOU Y J J , HILLWIG M L , et al . CYP76AH1 catalyzes turnover of miltiradiene in tanshinones biosynthesis and enables heterologous production of ferruginol in yeasts [J ] . Proceedings of the National Academy of Sciences of the United States of America , 2013 , 110 ( 29 ): 12108 - 12113 .

ZHOU Y J J , GAO W , RONG Q X , et al . Modular pathway engineering of diterpenoid synthases and the mevalonic acid pathway for miltiradiene production [J ] . Journal of The American Chemical Society , 2012 , 134 ( 6 ): 3234 - 3241 .

ZHAO Y J , FAN J J , WANG C , et al . Enhancing oleanolic acid production in engineered Saccharomyces cerevisiae [J ] . Bioresource Technology , 2018 , 257 : 339 - 343 .

ZHU M , WANG C X , SUN W T , et al . Boosting 11-oxo-ss-amyrin and glycyrrhetinic acid synthesis in Saccharomyces cerevisiae via pairing novel oxidation and reduction system from legume plants [J ] . Metabolic Engineering , 2018 , 45 : 43 - 50 .

BAI Y F , YIN H , BI H P , et al . De novo biosynthesis of gastrodin in Escherichia coli [J ] . Metabolic Engineering , 2016 , 35 : 138 - 147 .

BAI Y F , BI H P , ZHUANG Y B , et al . Production of salidroside in metabolically engineered Escherichia coli [J ] . Scientific Reports , 2014 , 4 : 6640 .

LIU X N , CHENG J , ZHANG G H , et al . Engineering yeast for the production of breviscapine by genomic analysis and synthetic biology approaches [J ] . Nature Communications , 2018 , 9 ( 1 ): 448 .

YAO Y F , WANG C S , QIAO J J , et al . Metabolic engineering of Escherichia coli for production of salvianic acid A via an artificial biosynthetic pathway [J ] . Metabolic Engineering , 2013 , 19 : 79 - 87 .

李宏彪 , 张国强 , 周景文 . 合成生物学在食品领域的应用 [J ] . 生物产业技术 , 2019 ( 4 ): 5 - 10 .

LI H B , ZHANG G Q , ZHOU J W . Applications of synthetic biology in food industry [J ] . Biotechnology & Business , 2019 ( 4 ): 5 - 10 .

TYAGI A , KUMAR A , APARNA S V , et al . Synthetic biology: applications in the food sector [J ] . Critical Reviews in Food Science and Nutrition , 2014 , 56 ( 11 ): 1777 - 1789 .

JIN Y , HE X Y , ANDOH-KUMI K , et al . Evaluating potential risks of food allergy and toxicity of soy leghemoglobin expressed in pichia pastoris [J ] . Molecular Nutrition & Food Research , 2018 , 62 ( 1 ): 1700297 .

陈坚 . 中国食品科技:从2020到2035 [J ] . 中国食品学报 , 2019 , 19 ( 12 ): 1 - 5 .

CHEN J . Food science and technology in China: 2020 to 2035 [J ] . Chinese Journal of Food , 2019 , 19 ( 12 ): 1 - 5 .

ZENG A P . New bioproduction systems for chemicals and fuels: needs and new development [J ] . Biotechnology Advances , 2019 , 37 ( 4 ): 508 - 518 .

WANG Y , FAN L , TUYISHIME P , et al . Synthetic methylotrophy: a practical solution for methanol-based biomanufacturing [J ] . Trends in biotechnology , 2020 , 38 ( 6 ): 650 - 666 .

MEYER F , KELLER P , HARTL J , et al . Methanol-essential growth of Escherichia coli [J ] . Nature Communications , 2018 , 9 : 1508 .

BENNETT R K , DILLON M , GERALD HAR J R , et al . Engineering Escherichia coli for methanol-dependent growth on glucose for metabolite production [J ] . Metabolic Engineering , 2020 , 60 : 45 - 55 .

TUYISHIME P , WANG Y , FAN L W , et al . Engineering corynebacterium glutamicum for methanol-dependent growth and glutamate production [J ] . Metabolic Engineering , 2018 , 49 : 220 - 231 .

KIM S , LINDNER S N , ASLAN S , et al . Growth of E. coli on formate and methanol via the reductive glycine pathway [J ] . Nature Chemical Biology , 2020 , 16 ( 5 ): 538 .

YISHAI O , BOUZON M , DORING V , et al . in Vivo assimilation of one-carbon via a synthetic reductive glycine pathway in Escherichia coli [J ] . ACS Synthetic Biology , 2018 , 7 ( 9 ): 2023 - 2028 .

TASHIRO Y , HIRANO S , MATSON M M , et al . Electrical-biological hybrid system for CO 2 reduction [J ] . Metabolic Engineering , 2018 , 47 : 211 - 218 .

GONG F Y , CAI Z , LI Y . Synthetic biology for CO 2 fixation [J ] . Science China Life Sciences , 2016 , 59 ( 11 ): 1106 - 1114 .

HU P , CHAKRABORTY S , KUMAR A , et al . Integrated bioprocess for conversion of gaseous substrates to liquids [J ] . Proceedings of the National Academy of Sciences of the United States of America , 2016 , 113 ( 14 ): 3773 - 3778 .

ZHU H W , MENG H K , ZHANG W , et al . Development of a longevous two-species biophotovoltaics with constrained electron flow [J ] . Nature Communications , 2019 , 10 ( 1 ): 4282 .

SCHWANDER T , VON BORZYSKOWSKI L S , BURGENER S , et al . A synthetic pathway for the fixation of carbon dioxide in vitro [J ] . Science , 2016 , 354 ( 6314 ): 900 - 904 .

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