基于工程化盐单胞菌的下一代工业生物技术

陈江楠; 陈潇宁; 刘心怡; 万薇; 章义鑫; 张自豪; 郑逸飞; 郑陶然; 王宣; 王子瑜; 闫煦; 张旭; 吴赴清; 陈国强

doi:10.12211/2096-8280.2020-042

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基于工程化盐单胞菌的下一代工业生物技术

特约评述 | 更新时间：2025-03-20

- 基于工程化盐单胞菌的下一代工业生物技术
- Engineering Halomonas spp. for next generation industrial biotechnology （NGIB）
- 合成生物学 2020年1卷第5期页码：516-527
- 作者机构：
  
  1.清华大学生命科学学院，北京 100084
  2.清华大学新雅书院，北京 100084
  3.天津大学生命科学学院，天津 300072
  4.山东大学生命科学学院，山东济南 250100
- 作者简介：
  
  吴赴清（1978—），男，博士，副研究员，主要从事PHA合成调控研究。E-mail：wufuqing@tsinghua.edu.cn
- 基金信息：
  
  国家重点研发计划“合成生物学”重点专项(2018YFA0900200)
- DOI：10.12211/2096-8280.2020-042
  中图分类号： Q 819
- 收稿：2020-04-07，
  
  修回：2020-05-06，
  
  纸质出版：2020-10-31
- 稿件说明：
移动端阅览
陈江楠, 陈潇宁, 刘心怡, 万薇, 章义鑫, 张自豪, 郑逸飞, 郑陶然, 王宣, 王子瑜, 闫煦, 张旭, 吴赴清, 陈国强. 基于工程化盐单胞菌的下一代工业生物技术[J]. 合成生物学, 2020, 1(5): 516-527

CHEN Jiangnan, CHEN Xiaoning, LIU Xinyi, WAN Wei, ZHANG Yixin, ZHANG Zihao, ZHENG Yifei, ZHENG Taoran, WANG Xuan, WANG Ziyu, YAN Xu, ZHANG Xu, WU Fuqing, CHEN Guoqiang. Engineering Halomonas spp. for next generation industrial biotechnology (NGIB)[J]. Synthetic Biology Journal, 2020, 1(5): 516-527
陈江楠, 陈潇宁, 刘心怡, 万薇, 章义鑫, 张自豪, 郑逸飞, 郑陶然, 王宣, 王子瑜, 闫煦, 张旭, 吴赴清, 陈国强. 基于工程化盐单胞菌的下一代工业生物技术[J]. 合成生物学, 2020, 1(5): 516-527 DOI： 10.12211/2096-8280.2020-042.

CHEN Jiangnan, CHEN Xiaoning, LIU Xinyi, WAN Wei, ZHANG Yixin, ZHANG Zihao, ZHENG Yifei, ZHENG Taoran, WANG Xuan, WANG Ziyu, YAN Xu, ZHANG Xu, WU Fuqing, CHEN Guoqiang. Engineering Halomonas spp. for next generation industrial biotechnology (NGIB)[J]. Synthetic Biology Journal, 2020, 1(5): 516-527 DOI： 10.12211/2096-8280.2020-042.

摘要

我国是工业生物技术大国，拥有世界上最大的发酵产业，但传统发酵需要灭菌操作，发酵过程高耗能、耗淡水且不能连续，导致生产成本偏高，无法与化学工业竞争。因此，急需开发下一代工业生物技术（NGIB）来克服这些缺点。NGIB利用盐单胞菌等极端微生物作为底盘细胞，具有发酵不需灭菌、节能节水、设备投资少、产物终浓度高、分离过程简单等优点。本文围绕下一代工业生物技术的发展过程，系统介绍了近年来在技术优势强化，生物元件和工具开发如Porin启动子系统、CRISPRi系统、CRISPR-Cas9系统等，新产物合成如聚（3-羟基丁酸-

-3-羟基戊酸）（PHBV）、聚（3-羟基丁酸-

-4-羟基丁酸）（P3HB4HB）、表面活性剂蛋白等，以及PHA分离过程优化、发酵工艺放大、废水循环利用等方面取得的最新进展。随着合成生物学发展和应用，基于工程化盐单胞菌的下一代工业生物技术体系正在不断完善，优势也愈加明显。下一代工业生物技术将为大幅提升绿色生物制造的竞争力提供强力支撑。

Abstract

China is a country with the largest industrial biotechnology capacity especially fermentation. However

it requires complicated sterilization procedures

high consumption of fresh water and energy

and expensive wastewater treatment processes. Therefore

it is urgent to develop the "Next Generation Industrial Biotechnology (NGIB)". The NGIB based on

Halomonas

spp. has many advantages

including: (1) energy-saving: no need for sterilization at high temperature and high pressure; (2) water-saving: use seawater instead of fresh water

and water can be recycled; (3) time-saving: production process can be continuous; (4) less investment in equipment: no need to use stainless steel fermentation system

instead

plastic

ceramics or even cement can be used as bioreactors; (5) high conce

ntration of final product

bacteria can produce products at a high concentration; (6) simplification of separation process: increase bacterial volume or surface charge

which is conducive to gravity or self-flocculation precipitation. Based on the newly developed ‘Next Generation Industrial Biotechnology'

this paper systematically introduces the latest progress in recent years in the fields such as strengthening technical advantages

development of biobricks and control parts including the porin promoter library

CRISPRi

CRISPR/Cas9 gene editing system

production of new products including poly(3-hydroxybutyrate-

-3-hydroxyvalerate)(PHBV)

Poly(3-hydroxybutyrate-

-4-hydroxybutyrate)(P3HB4HB) and Bio-surfactant Protein PhaP

optimization of separation process

scale up of fermentation processes

and recycling of wastewater. With the application of synthetic biology

the efficiency of NGIB has been continuously improved

and its advantages are obvious. Nevertheless

NGIB also faces some technical challenges

particularly treatment of salt containing wastewater. NGIB will further improve the recycling strategy of high salt wastewater

develop control modules under high cell density fermentation conditions

strengthen the design of low-cost substrate utilization ways

expand the scale and application fields

and achieve the mass production of various chemicals. The continuous improvement of NGIB will provide strong support for the competitiveness of green bio-manufacturing.

关键词

Keywords

references

李雪静 . 新形势下炼油工业发展新动向及新挑战 [J ] . 石化技术与应用 , 2019 , 37 ( 4 ): 225 - 229 .

LI Xuejing . New trends and challenges of refining industry in the new situation [J ] . Petrochemical Technology & Application , 2019 , 37 ( 4 ): 225 - 229 .

余春浩 . 石油泄漏对土壤的污染 [J ] . 当代化工 , 2019 , 48 ( 10 ): 2385 - 2387 .

YU Chunhao . Review of soil pollution in petrochemical industry [J ] . Contemporary Chemical Industry , 2019 , 48 ( 10 ): 2385 - 2387 .

VENKATESH A , POSEN I D , MACLEAN H L , et al . Environmental aspects of biotechnology [J ] . Advances in Biochemical Engineering Biotechnology , 2020 , 173 : 77 - 119 .

OTERO J M , NIELSEN J . Industrial systems biology [J ] . Biotechnology and Bioengineering , 2010 , 105 ( 3 ): 439 - 460 .

YU Linping , WU Fuqing , CHEN Guoqiang . Next-generation industrial biotechnology - transforming the current industrial biotechnology into competitive processes [J ] . Biotechnology Journal , 2019 , 14 ( 9 ): e1800437 .

CHEN Zhu , WAN Caixia . Non-sterile fermentations for the economical biochemical conversion of renewable feedstocks [J ] . Biotechnology Letters , 2017 , 39 ( 12 ): 1765 - 1777 .

PHILP J C , RITCHIE R J , ALLAN J E . Biobased chemicals: the convergence of green chemistry with industrial biotechnology [J ] . Trends in Biotechnology , 2013 , 31 ( 4 ): 219 - 222 .

RADDADI N , CHERIF A , DAFFONCHIO D , et al . Biotechnological applications of extremophiles, extremozymes and extremolytes [J ] . Applied Microbiology and Biotechnology , 2015 , 99 ( 19 ): 7907 - 7913 .

CHEN Guoqiang , JIANG Xiaoran . Next generation industrial biotechnology based on extremophilic bacteria [J ] . Current Opinion in Biotechnology , 2018 , 50 : 94 - 100 .

YIN Jin , CHEN Jinchun , WU Qiong , et al . Halophiles, coming stars for industrial biotechnology [J ] . Biotechnology Advances , 2015 , 33 ( 7 ): 1433 - 1442 .

CAI Lei , TAN Dan , AIBAIDULA G , et al . Comparative genomics study of polyhydroxyalkanoates (PHA) and ectoine relevant genes from Halomonas sp. TD01 revealed extensive horizontal gene transfer events and co-evolutionary relationships [J ] . Microbial Cell Factories , 2011 , 10 : 88 .

KAWATA Y , KAWASAKI K , SHIGERI Y . Draft genome sequence of Halomonas sp. strain KM-1, a moderately halophilic bacterium that produces the bioplastic poly(3-hydroxybutyrate) [J ] . Journal of Bacteriology , 2012 , 194 ( 10 ): 2738 - 2739 .

LIN Yanbing , FAN Haoxin , HAO Xiuli , et al . Draft genome sequence of Halomonas sp. strain HAL1, a moderately halophilic arsenite-oxidizing bacterium isolated from gold-mine soil [J ] . Journal of Bacteriology , 2011 , 194 ( 1 ): 199 - 200 .

PHUNG LE T , SILVER S , TRIMBLE W L , et al . Draft genome of Halomonas species strain GFAJ-1 (ATCC BAA-2256) [J ] . Journal of Bacteriology , 2012 , 194 ( 7 ): 1835 - 1836 .

SCHWIBBERT K , MARIN-SANGUINO A , BAGYAN I , et al . A blueprint of ectoine metabolism from the genome of the industrial producer Halomonas elongata DSM 2581T [J ] . Environmental Microbiology , 2011 , 13 ( 8 ): 1973 - 1994 .

ZHENG Yang , CHEN Jinchun , MA Yiming , et al . Engineering biosynthesis of polyhydroxyalkanoates (PHA) for diversity and cost reduction [J ] . Metabolic Engineering , 2020 , 58 : 82 - 93 .

CHEN Yong , CHEN Xinyu , DU Hetong , et al . Chromosome engineering of the TCA cycle in Halomonas bluephagenesis for production of copolymers of 3-hydroxybutyrate and 3-hydroxyvalerate (PHBV) [J ] . Metabolic Engineering , 2019 , 54 : 69 - 82 .

YE Jianwen , HU Dingkai , CHE Xuemei , et al . Engineering of Halomonas bluephagenesis for low cost production of poly(3-hydroxybutyrate- co -4-hydroxybutyrate) from glucose [J ] . Metabolic Engineering , 2018 , 47 : 143 - 152 .

LAN Luhong , ZHAO Han , CHEN Jinchun , et al . Engineering Halomonas spp. as a low-cost production host for production of bio-surfactant protein PhaP [J ] . Biotechnology Journal , 2016 , 11 ( 12 ): 1595 - 1604 .

LI Tian , GUO Yingying , QIAO Guanqing , et al . Microbial synthesis of 5-aminolevulinic acid and its coproduction with polyhydroxybutyrate [J ] . ACS Synthetic Biology , 2016 , 5 ( 11 ): 1264 - 1274 .

杨博 , 南昊 . 我国水资源现状及其安全对策研究 [J ] . 太原学院学报(自然科学版) , 2016 , 34 ( 1 ): 9 - 12 .

YANG Bo , NAN Hao . Research on the present situation of China's water resources and its security countermeasures [J ] . Journal of Taiyuan University (Natural Science Edition) , 2016 , 34 ( 1 ): 9 - 12 .

姜祖岩 . 我国海水利用产业发展形势与存在问题分析 [J ] . 海洋经济 , 2019 , 9 ( 1 ): 20 - 28 .

JIANG Zuyan . Analysis on the development situation and existing problems of China's seawater utilization industry [J ] . Marine Economy , 2019 , 9 ( 1 ): 20 - 28 .

CHEN Guoqiang . New challenges and opportunities for industrial biotechnology [J ] . Microbial Cell Factories , 2012 , 11 : 111 .

JIANG Xiaoran , YIN Jin , CHEN Xiangbin , et al . Halomonas and pathway engineering for bioplastics production [J ] . Methods in Enzymology , 2018 , 608 : 309 - 328 .

TOHME S , HACIOSMANOGLU G G , EROGLU M S , et al . Halomonas smyrnensis as a cell factory for co-production of PHB and levan [J ] . International Journal of Biological Macromolecules , 2018 , 118 ( A ): 1238 - 1246 .

JOULAK I , FINORE I , NICOLAUS B , et al . Evaluation of the production of exopolysaccharides by newly isolated Halomonas strains from Tunisian hypersaline environments [J ] . International Journal of Biological Macromolecules , 2019 , 138 : 658 - 666 .

ZHAO Qi , LI Shannan , LÜ Peiwen , et al . High ectoine production by an engineered Halomonas hydrothermalis Y2 in a reduced salinity medium [J ] . Microbial Cell Factories , 2019 , 18 ( 1 ): 184 .

ADIMOOLAM S R , NANJAN E S , SUBRAMANIAN M , et al . Metabolic heat coherent growth of Halomonas variabilis (HV) for enhanced production of extracellular polymeric substances (EPS) in a bio reaction calorimeter (BioRC) [J ] . Preparative Biochemistry & Biotechnology , 2020 , 50 ( 1 ): 56 - 65 .

TAN D , XUE Y S , AIBAIDULA G , et al . Unsterile and continuous production of polyhydroxybutyrate by Halomonas TD01 [J ] . Bioresource Technology , 2011 , 102 ( 17 ): 8130 - 8136 .

YUE Haitao , LING Chen , YANG Tao , et al . A seawater-based open and continuous process for polyhydroxyalkanoates production by recombinant Halomonas campaniensis LS21 grown in mixed substrates [J ] . Biotechnology for Biofuels , 2014 , 7 : 108 .

KREYENSCHULTE D , KRULL R , MARGARITIS A . Recent advances in microbial biopolymer production and purification [J ] . Critical Reviews in Biotechnology , 2014 , 34 : 1 - 15 .

DON T M , CHEN C W , CHAN T H . Preparation and characterization of poly(hydroxyalkanoate) from the fermentation of Haloferax mediterranei [J ] . Journal of Biomaterials Science , Polymer Edition , 2006 , 1425 - 1438 .

JOHNSON K , JIANG Yang , KLEEREBEZEM R , et al . Enrichment of a mixed bacterial culture with a high polyhydroxyalkanoate storage capacity [J ] . Biomacromolecules , 2009 , 10 ( 4 ): 670 - 676 .

CARCAMO M , SAA P A , TORRES J , et al . Effective dissolved oxygen control strategy for high-cell-density cultures [J ] . IEEE Latin America Transactions , 2014 , 12 ( 3 ): 389 - 394 .

XUE Sijia , JIANG Hong , CHEN Lu , et al . Over-expression of Vitreoscilla hemoglobin (VHb) and flavohemoglobin (FHb) genes greatly enhances pullulan production [J ] . International Journal of Biological Macromolecules , 2019 , 132 : 701 - 709 .

MEJIA A , LUNA D , FERNANDEZ F J , et al . Improving rifamycin production in Amycolatopsis mediterranei by expressing a Vitreoscilla hemoglobin ( vhb ) gene fused to a cytochrome P450 monooxygenase domain [J ] . 3 Biotechnology , 2018 , 8 ( 11 ): 456 .

ZHANG Shumeng , WANG Jieping , WEI Yale , et al . Heterologous expression of VHb can improve the yield and quality of biocontrol fungus Paecilomyces lilacinus , during submerged fermentation [J ] . Journal of Biotechnology , 2014 , 187 : 147 - 153 .

OUYANG Pengfei , WANG Huan , HAJNAL I , et al . Increasing oxygen availability for improving poly(3-hydroxybutyrate) production by Halomonas [J ] . Metabolic Engineering , 2018 , 45 : 20 - 31 .

WU Hong , WANG Huan , CHEN Jinchun , et al . Effects of cascaded vgb promoters on poly(hydroxybutyrate) (PHB) synthesis by recombinant Escherichia coli grown micro-aerobically [J ] . Applied Microbiology and Biotechnology , 2014 , 98 ( 24 ): 10013 - 10021 .

朱晁谊 , 朱牧孜 , 李爽 . 微生物实验室进化的研究进展 [J ] . 生物加工过程 , 2019 , 17 ( 1 ): 8 - 14 .

ZHU Chaoyi , ZHU Muzi , LI Shuang . Research progress in microbial laboratory evolution [J ] . Chinese Journal of Bioprocess Engineering , 2019 , 17 ( 1 ): 8 - 14 .

贝泓涵 , 张立卫 , 孙菁 . 微生物连续发酵过程线性反馈最优控制 [J ] . 大连理工大学学报 , 2018 , 58 ( 3 ): 324 - 330 .

BEI Honghan , ZHANG Liwei , SUN Jing . Linear optimal feedback control in continuous culture of microbial fermentation [J ] . Journal of Dalian University of Technology , 2018 , 58 ( 3 ): 324 - 330 .

LI Teng , CHEN Xiangbin , CHEN Jinchun , et al . Open and continuous fermentation: products, conditions and bioprocess economy [J ] . Biotechnology Journal , 2014 , 9 ( 12 ): 1503 - 1511 .

LING Chen , QIAO Guanqing , SHUAI Bowen , et al . Engineering self-flocculating Halomonas campaniensis for wastewaterless open and continuous fermentation [J ] . Biotechnology and Bioengineering , 2018 , 116 : 805 - 815 .

邓姗 , 任元元 , 周远洁 . 浅谈工业化发酵产品成本工艺控制关键 [J ] . 食品与发酵科技 , 2019 , 55 ( 2 ): 78 - 80 .

DENG Shan , REN Yuanyuan , ZHOU Yuanjie . Key points of cost control in industrial fermentation products [J ] . Food and Fermentation Science & Technology , 2019 , 55 ( 2 ): 78 - 80 .

TAN Dan , WU Qiong , CHEN Jinchun , et al . Engineering Halomonas TD01 for the low-cost production of polyhydroxyalkanoates [J ] . Metabolic Engineering , 2014 , 26 : 34 - 47 .

SABAPATHY P C , DEVARAJ S , PARTHIPAN A , et al . Polyhydroxyalkanoate production from statistically optimized media using rice mill effluent as sustainable substrate with an analysis on the biopolymer's degradation potential [J ] . International Journal of Biological Macromolecules , 2019 , 126 : 977 - 986 .

AL-SHORGANI N K N , AL-TABIB A I , KADIER A , et al . Continuous butanol fermentation of dilute acid-pretreated de-oiled rice bran by Clostridium acetobutylicum YM1 [J ] . Scientific Reports , 2019 , 9 ( 1 ): 4622 .

FU X Z , TAN D , AIBAIDULA G , et al . Development of Halomonas TD01 as a host for open production of chemicals [J ] . Metabolic Engineering , 2014 , 23 : 78 - 91 .

YIN Jin , FU Xiaozhi , WU Qiong , et al . Development of an enhanced chromosomal expression system based on porin synthesis operon for halophile Halomonas sp . [J ] . Applied Microbiology and Biotechnology , 2014 , 98 ( 21 ): 8987 - 8997 .

LI Tingting , LI Teng , JI Weiyue , et al . Engineering of core promoter regions enables the construction of constitutive and inducible promoters in Halomonas sp . [J ] . Biotechnology Journal , 2016 , 11 ( 2 ): 219 - 227 .

SHEN Rui , YIN Jin , YE Jianwen , et al . Promoter engineering for enhanced P(3HB- co -4HB) production by Halomonas bluephagenesis [J ] . ACS Synthetic Biology , 2018 , 7 ( 8 ): 1897 - 1906 .

TAO Wei , LV Li , CHEN Guoqiang . Engineering Halomonas species TD01 for enhanced polyhydroxyalkanoates synthesis via CRIS PRi [J ] . Microbial Cell Factories , 2017 , 16 ( 1 ): 48 .

ZHAO Han , ZHANG Haoqian M , CHEN Xiangbin , et al . Novel T7-like expression systems used for Halomonas [J ] . Metabolic Engineering , 2017 , 39 : 128 - 140 .

QIN Qin , LING Chen , ZHAO Yiqing , et al . CRISPR/Cas9 editing genome of extremophile Halomonas spp . [J ] . Metabolic Engineering , 2018 , 47 : 219 - 229 .

LING Chen , QIAO Guanqing , SHUAI Bowen , et al . Engineering NADH/NAD + ratio in Halomonas bluephagenesis for enhanced production of polyhydroxyalkanoates (PHA) [J ] . Metabolic Engineering , 2018 , 49 : 275 - 286 .

CHEN Xiangbin , YIN Jin , YE Jianwen , et al . Engineering Halomonas bluephagenesis TD01 for non-sterile production of poly(3-hydroxybutyrate- co -4-hydroxybutyrate) [J ] . Bioresource Technology , 2017 , 244 : 534 - 541 .

PENA C , CASTILLO T , GARCIA A , et al . Biotechnological strategies to improve production of microbial poly-(3-hydroxybutyrate): a review of recent research work [J ] . Microbial Biotechnology , 2014 , 7 ( 4 ): 278 - 293 .

RIVERA-BRISO A L , SERRANO-AROCA A . Poly(3-hydroxybutyrate- co -3-hydroxyvalerate): enhancement strategies for advanced applications [J ] . Polymers (Basel) , 2018 , 10 ( 7 ): 732 .

JIANG Xiaoran , YAO Zhihao , CHEN Guoqiang . Controlling cell volume for efficient PHB production by Halomonas [J ] . Metabolic Engineering , 2017 , 44 : 30 - 37 .

车雪梅 , 司徒卫 , 余柳松 , 等 . 聚羟基脂肪酸酯的应用展望 [J ] . 生物工程学报 , 2018 , 34 ( 10 ): 1531 - 1542 .

CHE Xuemei , SITU Wei , YU Liusong , et al . Application perspectives of polyhydroxyalkanoates [J ] . Chinese Journal of Biotechnology , 2018 , 34 ( 10 ): 1531 - 1542 .

HAJNAL I , CHEN X B , CHEN G Q . A novel cell autolysis system for cost-competitive downstream processing [J ] . Applied Microbiology and Biotechnology , 2016 , 100 ( 21 ): 9103 - 9110 .

YE Jianwen , HUANG Wuzhe , WANG Dongsheng , et al . Pilot scale-up of poly(3-hydroxybutyrate- co -4-hydroxybutyrate) production by Halomonas bluephagenesis via cell growth adapted optimization process [J ] . Biotechnology Journal , 2018 , 13 ( 5 ): e1800074 .

ZHANG C R , BOOP F A , RUGE J . The use of 5-aminolevulinic acid in resection of pediatric brain tumors: a critical review [J ] . Journal of Neuro-Oncology , 2019 , 141 ( 3 ): 567 - 573 .

WAINWRIGHT J V , ENDO T , COOPER J B , et al . The role of 5-aminolevulinic acid in spinal tumor surgery: a review [J ] . Journal of Neuro-Oncology , 2019 , 141 ( 3 ): 575 - 584 .

KANG Zhen , DING Wenwen , GONG Xu , et al . Recent advances in production of 5-aminolevulinic acid using biological strategies [J ] . World Journal of Microbiology and Biotechnology , 2017 , 33 ( 11 ): 200 .

BISSON A W , HSU YEN-PANG , SQUYRES G R , et al . Treadmilling by FtsZ filaments drives peptidoglycan synthesis and bacterial cell division [J ] . Science , 2017 , 355 ( 6326 ): 739 - 743 .

HUSSAIN S , WIVAGG C N , SZWEDZIAK P , et al . MreB filaments align along greatest principal membrane curvature to orient cell wall synthesis [J ] . eLife , 2018 , 7 : e32471 .

SHI H D , BRATTON B P , GITAI Z , et al . How to build a bacterial cell: MreB as the foreman of E. coli construction [J ] . Cell , 2018 , 172 ( 6 ): 1294 - 1305 .

LIU Yunqi , YIN Shengli , WANG Yujie , et al . Effect of high porosity on biodegradation of poly (4-hydroxybutyrate) in vivo [J ] . Journal of Biomaterials Applications , 2014 , 28 ( 7 ): 1105 - 1112 .

JIANG Xiaoran , WANG Huan , SHEN Rui , et al . Engineering the bacterial shapes for enhanced inclusion bodies accumulation [J ] . Metabolic Engineering , 2015 , 29 : 227 - 237 .

ELHADI D , LV L , JIANG X R , et al . CRISPRi engineering E. coli for morphology diversification [J ] . Metabolic Engineering , 2016 , 38 : 358 - 369 .

SHEN Rui , NING Zhiyu , LAN Yuxuan , et al . Manipulation of polyhydroxyalkanoate granular sizes in Halomonas bluephagenesis [J ] . Metabolic Engineering , 2019 , 54 : 117 - 126 .

ZHANG Xu , LIN Yina , CHEN Guoqiang . Halophiles as chassis for bioproduction [J ] . Advanced Biosystems , 2018 , 2 : 1 - 12 .

陈则立 , 李彤 , 朱华静 . 阳离子交换膜在高盐废水处理中的应用 [J ] . 膜科学与技术 , 2019 , 39 ( 5 ): 136 - 142 .

CHEN Zeli , LI Tong , ZHU Huajing . Application of cation exchange membrane in the treatment of high-salt wastewater [J ] . Membrane Science and Technology , 2019 , 39 ( 5 ): 136 - 142 .

曹美玲 , 李海 , 刘佛财 , 等 . 高盐有机废水的处理与研究进展 [J ] . 有色金属科学与工程 , 2019 , 10 ( 3 ): 92 - 98 .

CAO Meiling , LI Hai , LIU Focai , et al . Recent evelopment in the treatment process for high salt organic wastewater [J ] . Nonfenous Metals Science and Engineering , 2019 , 10 ( 3 ): 92 - 98 .

李俊虎 , 周珉 , 王乔 , 等 . 高盐废水处理工艺最新研究进展 [J ] . 环境科技 , 2018 , 31 ( 4 ): 74 .

LI Junhu , ZHOU Min , WANG Qiao , et al . The latest progress of treatment technology for high salinity wastewater [J ] . Environmental Science and Technology , 2018 , 31 ( 4 ): 74 .

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