枯草芽孢杆菌底盘细胞的设计、构建与应用

林璐; 吕雪芹; 刘延峰; 堵国成; 陈坚; 刘龙

doi:10.12211/2096-8280.2020-030

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枯草芽孢杆菌底盘细胞的设计、构建与应用

特约评述 | 更新时间：2025-03-20

- 枯草芽孢杆菌底盘细胞的设计、构建与应用
- Advances in design, construction and applications of Bacillus subtilis chassis cells
- 合成生物学 2020年1卷第2期页码：247-265
- 作者机构：
  
  1.江南大学糖化学与生物技术教育部重点实验室江苏无锡 214122
  2.江南大学工业生物技术教育部重点实验室江苏无锡 214122
- 作者简介：
  
  [ "林璐（1993—），女，博士研究生，研究方向为微生物代谢工程。E-mail：linlu@stu.jiangnan.edu.cn" ]
  [ "刘龙（1980—），男，博士，教授，研究方向为代谢工程与合成生物学。E-mail：longliu@jiangnan.edu.cn" ]
- 基金信息：
  
  国家自然科学基金优秀青年基金(31622001);国家重点研发计划(2018YFA0900300)
- DOI：10.12211/2096-8280.2020-030
  中图分类号： Q819
- 收稿：2020-03-13，
  
  修回：2020-04-08，
  
  纸质出版：2020-04-30
- 稿件说明：
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林璐, 吕雪芹, 刘延峰, 堵国成, 陈坚, 刘龙. 枯草芽孢杆菌底盘细胞的设计、构建与应用[J]. 合成生物学, 2020, 1(2): 247-265

LIN Lu, LV Xueqin, LIU Yanfeng, DU Guocheng, CHEN Jian, LIU Long. Advances in design,construction and applications of Bacillus subtilis chassis cells[J]. Synthetic Biology Journal, 2020, 1(2): 247-265
林璐, 吕雪芹, 刘延峰, 堵国成, 陈坚, 刘龙. 枯草芽孢杆菌底盘细胞的设计、构建与应用[J]. 合成生物学, 2020, 1(2): 247-265 DOI： 10.12211/2096-8280.2020-030.

LIN Lu, LV Xueqin, LIU Yanfeng, DU Guocheng, CHEN Jian, LIU Long. Advances in design,construction and applications of Bacillus subtilis chassis cells[J]. Synthetic Biology Journal, 2020, 1(2): 247-265 DOI： 10.12211/2096-8280.2020-030.

摘要

作为一种重要的模式工业微生物和食品安全微生物，枯草芽孢杆菌在工业酶和功能营养品的生产方面具有广泛应用。近年来，随着对枯草芽孢杆菌遗传调控机制的不断揭示，多种研究策略和技术，包括基因编辑系统、基因回路、空间支架、无细胞表达系统等，被用于设计和构建枯草芽孢杆菌底盘细胞高效合成各种生物化学品。本文首先对基于基因编辑和基于内源性调控系统的枯草芽孢杆菌底盘细胞设计与构建的策略进行了系统的概述。然后，以高产

-乙酰氨基葡萄糖、七烯甲萘醌、核黄素、透明质酸和β-环糊精糖基转移酶为例介绍了枯草芽孢杆菌底盘细胞工厂的应用。最后，从基因编辑效率提升、基因回路响应信号拓展和基因组设计与重构三方面对枯草芽孢杆菌底盘细胞的设计、构建与应用进行了展望。

Abstract

As a model industrial host and an important generally recognized as safe microorganism

Bacillus subtilis

has been used for a wide range of applications such as the industrial production of enzymes and nutraceuticals. In recent years

with the elucidation of the genetic regulation mechanism of

B. subtilis

various research strategies and technologies have been designed and developed with this chassis

including gene editing

gene circuits

spatial biomolecular scaffold and cell-free expression systems. In this review

we start with systematic summaries on the co

nstruction of

B. subtilis

chassis based on gene editing systems and endogenous regulatory mechanisms. Then applications of

B. subtilis

cell factories are discussed for producing

-acetylglucosamine

menaquinone-7

riboflavin

hyaluronic acid and β-cyclodextrin glycosyltransferase. Finally

prospects for the design

construction and applications of engineered

B. subtilis

strains are commented

with an emphasis on improving genome editing efficiency

expanding responsive metabolite spectrum for genetic circuits

and rewiring the whole genome.

关键词

Keywords

references

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