甲醇生物转化的机遇与挑战

高教琪; 周雍进

doi:10.12211/2096-8280.2020-017

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甲醇生物转化的机遇与挑战

特约评述 | 更新时间：2025-03-20

- 甲醇生物转化的机遇与挑战
- Advances in methanol bio-transformation
- 合成生物学 2020年1卷第2期页码：158-173
- 作者机构：
  
  1.中国科学院大连化学物理研究所生物技术研究部，辽宁大连 116023
  2.大连市能源生物技术重点实验室，辽宁大连 116023
- 作者简介：
  
  [ "高教琪（1989—），男，博士，助理研究员，主要从事多形汉逊酵母甲醇生物转化及产物合成研究。E-mail：jqgao@dicp.ac.cn" ]
  [ "周雍进（1984—），男，博士，研究员，主要从事基于合成生物学工具开发的甲醇生物转化与天然产物合成研究。E-mail：zhouyongjin@dicp.ac.cn" ]
- 基金信息：
  
  国家自然科学基金青年项目(21808216;31700082);国家自然科学基金优秀青年基金项目(21922812);中国科学院大连化学物理研究所BioChE-X项目(BioChE-X201801)
- DOI：10.12211/2096-8280.2020-017
  中图分类号： Q815
- 收稿：2020-03-05，
  
  修回：2020-03-23，
  
  纸质出版：2020-04-30
- 稿件说明：
移动端阅览
高教琪, 周雍进. 甲醇生物转化的机遇与挑战[J]. 合成生物学, 2020, 1(2): 158-173

GAO Jiaoqi, ZHOU Yongjin. Advances in methanol bio-transformation[J]. Synthetic Biology Journal, 2020, 1(2): 158-173
高教琪, 周雍进. 甲醇生物转化的机遇与挑战[J]. 合成生物学, 2020, 1(2): 158-173 DOI： 10.12211/2096-8280.2020-017.

GAO Jiaoqi, ZHOU Yongjin. Advances in methanol bio-transformation[J]. Synthetic Biology Journal, 2020, 1(2): 158-173 DOI： 10.12211/2096-8280.2020-017.

摘要

甲醇以其易储存和易运输等特性，成为极具应用潜力的原料。除了传统的基于合成气（CO，H

，CO

）和化学催化的甲醇合成，甲烷氧化（化学法和生物法）和CO

加氢技术逐步成熟，特别是CO

加氢技术将有望实现甲醇可持续洁净合成。甲醇生物转化有望进一

步拓展现有甲醇转化路线，推动我国煤炭资源洁净利用以及CO

利用。本文详细综述了国内外甲醇生物炼制研究进展：①以甲基营养型微生物，包括细菌和甲醇酵母，为宿主构建细胞工厂实现氨基酸以及平台化合物等合成；②在模式微生物中构建甲醇代谢途径实现甲醇利用与转化。通过天然/人工甲基营养型代谢途径相互借鉴，采用代谢工程与合成生物学策略，提高甲醇利用效率、底物与产物耐受能力，将推动甲醇生物转化、拓展生物炼制原料供应路线。

Abstract

Methanol represents a promising feedstock due to its specific characteristics for storage and transport

which has been widely applied in chemical industry as raw material

intermediate and fuel. Methanol bio-transformation by microbes may further expand the methanol-based production of high quality fuels and various chemicals

which will drive clean utilization of inferior coal and natural gas. We here review recent advances in methanol-based bio-refinery. We first summarize current progress in methanol production through chemical and biological processes

which shows that the chemical process is now the main route for methanol supply. However

emerging technologies like methane oxidation (chemical or biological) and CO

hydrogenation may achieve a renewable

sustainable and clean route for methanol production. We then discuss the engineering of methylotrophs (bacterial and yeast) for producing a variety of chemicals from methanol (Top-Down approach)

which indicates that improved genetic tools may further optimize these cell factories for industrial applications. We also summarize recent advances on engineering artificial methylotrophs in numerous model organisms such as

Escherichia coli

Corynebacterium glutamicum

and

Saccharomyces cerevisiae

(Bottom-Up approach). The advances in synthetic biology

metabolic engineering and adaptive laboratory evolution will facilitate the construction of robust microbial cell factories for methanol biotransformation

which will expand substrate resource for bio-refinery and the product portfolio of methanol.

关键词

Keywords

references

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