FRYER M J . The antioxidant effects of thylakoid vitamin E (α-tocopherol) [J ] . Plant , Cell & Environment, 1992 , 15 ( 4 ): 381 - 392 .

MATRINGE M , KSAS B , REY P , et al . Tocotrienols, the unsaturated forms of vitamin E, can function as antioxidants and lipid protectors in tobacco leaves [J ] . Plant Physiology , 2008 , 147 ( 2 ): 764 - 778 .

JOMOVA K , RAPTOVA R , ALOMAR S Y , et al . Reactive oxygen species, toxicity, oxidative stress, and antioxidants: chronic diseases and aging [J ] . Archives of Toxicology , 2023 , 97 ( 10 ): 2499 - 2574 .

BOREL P , PREVERAUD D , DESMARCHELIER C . Bioavailability of vitamin E in humans: an update [J ] . Nutrition Reviews , 2013 , 71 ( 6 ): 319 - 331 .

JIANG Q . Metabolism of natural forms of vitamin E and biological actions of vitamin E metabolites [J ] . Free Radical Biology and Medicine , 2022 , 179 : 375 - 387 .

DELLAPENNA D , POGSON B J . VITAMIN SYNTHESIS IN PLANTS: Tocopherols and carotenoids [J ] . Annual Review of Plant Biology , 2006 , 57 : 711 - 738 .

MÈNE-SAFFRANÉ L . Vitamin E biosynthesis and its regulation in plants [J ] . Antioxidants , 2018 , 7 ( 1 ): 2 .

TRABER M G , ATKINSON J . Vitamin E, antioxidant and nothing more [J ] . Free Radical Biology and Medicine , 2007 , 43 ( 1 ): 4 - 15 .

BURTON G W , TRABER M G , ACUFF R V , et al . Human plasma and tissue alpha-tocopherol concentrations in response to supplementation with deuterated natural and synthetic vitamin E [J ] . The American Journal of Clinical Nutrition , 1998 , 67 ( 4 ): 669 - 684 .

KAMAL-ELDIN A , APPELQVIST L-Å . The chemistry and antioxidant properties of tocopherols and tocotrienols [J ] . Lipids , 1996 , 31 ( 7 ): 671 - 701 .

SALIMATH S S , ROMSDAHL T B , KONDA A R , et al . Production of tocotrienols in seeds of cotton ( Gossypium hirsutum L.) enhances oxidative stability and offers nutraceutical potential [J ] . Plant Biotechnology Journal , 2021 , 19 ( 6 ): 1268 - 1282 .

YANG J , YU H , YE L . Evolution of vitamin E production: From chemical synthesis and plant extraction to microbial cell factories [J ] . Journal of Agricultural and Food Chemistry , 2024 , 72 ( 50 ): 27701 - 27714 .

ZHANG R , REN Y , ZHAO Y , et al . A century of vitamin E research: The innovative journey from basic biology to synthetic bio-manufacturing [J ] . Journal of Integrative Plant Biology , 2026 , 68 ( 4 ): 985 - 1012 .

ALBERMANN C , GHANEGAONKAR S , LEMUTH K , et al . Biosynthesis of the vitamin E compound δ-tocotrienol in recombinant Escherichia coli cells [J ] . ChemBioChem , 2008 , 9 ( 15 ): 2524 - 2533 .

GHANEGAONKAR S , CONRAD J , BEIFUSS U , et al . Towards the in vivo production of tocotrienol compounds: Engineering of a plasmid-free Escherichia coli strain for the heterologous synthesis of 2-methyl-6-geranylgeranyl-benzoquinol [J ] . Journal of Biotechnology , 2013 , 164 ( 2 ): 238 - 247 .

SUN H , YANG J , LIN X , et al . De novo high-titer production of delta-tocotrienol in recombinant Saccharomyces cerevisiae [J ] . Journal of Agricultural and Food Chemistry , 2020 , 68 ( 29 ): 7710 - 7717 .

SHEN B , ZHOU P , JIAO X , et al . Fermentative production of vitamin E tocotrienols in Saccharomyces cerevisiae under cold-shock-triggered temperature control [J ] . Nature Communications , 2020 , 11 ( 1 ): 5155 .

JIAO X , SHEN B , LI M , et al . Secretory production of tocotrienols in Saccharomyces cerevisiae [J ] . ACS Synthetic Biology , 2022 , 11 ( 2 ): 788 - 799 .

HAN L , WU Y , XU Y , et al . Engineered Saccharomyces cerevisiae for de novo δ-tocotrienol biosynthesis [J ] . Systems Microbiology and Biomanufacturing , 2024 , 4 ( 1 ): 150 - 164 .

JIAO X , BIAN Q , FENG T , et al . Efficient secretory production of δ-tocotrienol by combining pathway modularization and transportation engineering [J ] . Journal of Agricultural and Food Chemistry , 2023 , 71 ( 23 ): 9020 - 9030 .

XIANG J , LIU M , WANG X , et al . Combined metabolic and enzymatic engineering for de novo biosynthesis of δ-tocotrienol in Yarrowia lipolytica [J ] . Synthetic and Systems Biotechnology , 2025 , 10 ( 3 ): 719 - 727 .

XU C , JIAO X , ZHAO Q , et al . Membrane transporter mining and enzyme engineering drive high-titer δ-tocotrienol production in Yarrowia lipolytica [J ] . Biochemical Engineering Journal , 2026 , 228 : 110079 .

ZHANG Y , LI Z , CAO L , et al . A nonclassical biosynthetic route in bacteria towards tocopherol synthesis [J ] . Nature Metabolism , 2026 , doi: 10.1038/s42255-026-01576-y http://dx.doi.org/10.1038/s42255-026-01576-y .

SAVIDGE B , WEISS J D , WONG Y H H , et al . Isolation and characterization of homogentisate phytyltransferase genes from Synechocystis sp. PCC 6803 and Arabidopsis [J ] . Plant Physiology , 2002 , 129 ( 1 ): 321 - 332 .

COLLAKOVA E , DELLAPENNA D . Homogentisate phytyltransferase activity is limiting for tocopherol biosynthesis in Arabidopsis [J ] . Plant Physiology , 2003 , 131 ( 2 ): 632 - 642 .

COLLAKOVA E , DELLAPENNA D . The role of homogentisate phytyltransferase and other tocopherol pathway enzymes in the regulation of tocopherol synthesis during abiotic stress [J ] . Plant Physiology , 2003 , 133 ( 2 ): 930 - 940 .

CAHOON E B , HALL S E , RIPP K G , et al . Metabolic redesign of vitamin E biosynthesis in plants for tocotrienol production and increased antioxidant content [J ] . Nature Biotechnology , 2003 , 21 ( 9 ): 1082 - 1087 .

KELLER Y , BOUVIER F , D'HARLINGUE A , CAMARA B . Metabolic compartmentation of plastid prenyllipid biosynthesis [J ] . European Journal of Biochemistry , 1998 , 251 ( 1-2 ): 413 - 417 .

HORVATH G , WESSJOHANN L , BIGIRIMANA J , et al . Differential distribution of tocopherols and tocotrienols in photosynthetic and non-photosynthetic tissues [J ] . Phytochemistry , 2006 , 67 ( 12 ): 1185 - 1195 .

COLLAKOVA E , DELLAPENNA D . Isolation and functional analysis of homogentisate phytyltransferase from Synechocystis sp. PCC 6803 and Arabidopsis [J ] . Plant Physiology , 2001 , 127 ( 3 ): 1113 - 1124 .

SATTLER S E , CAHOON E B , COUGHLAN S J , et al . Characterization of tocopherol cyclases from higher plants and cyanobacteria: Evolutionary implications for tocopherol synthesis and function [J ] . Plant Physiology , 2003 , 132 ( 4 ): 2184 - 2195 .

SHINTANI D , DELLAPENNA D . Elevating the vitamin E content of plants through metabolic engineering [J ] . Science , 1998 , 282 ( 5396 ): 2098 - 2100 .

ZHANG C , CAHOON R E , HUNTER S C , et al . Genetic and biochemical basis for alternative routes of tocotrienol biosynthesis for enhanced vitamin E antioxidant production [J ] . The Plant Journal , 2013 , 73 ( 4 ): 628 - 639 .

KEASLING J D . Manufacturing molecules through metabolic engineering [J ] . Science , 2010 , 330 ( 6009 ): 1355 - 1358 . [35 ] NIELSEN J, KEASLING J D. Engineering cellular metabolism [J ] . Cell, 2016 , 164 ( 6 ): 1185 - 1197 .

QI Q G , HAO M , NG W O , et al . Application of the Synechococcus nirA promoter to establish an inducible expression system for engineering the Synechocystis tocopherol pathway [J ] . Applied and Environmental Microbiology , 2005 , 71 ( 10 ): 5678 - 5684 .

王岩岩 , 刘林霞 , 金朝霞 , 等 . 代谢工程在维生素生产中的应用及研究进展 [J ] . 生物工程学报 , 2021 , 37 ( 5 ): 1748 - 1770 .

WANG Y Y , LIU L X , JIN Z X , et al . Advances in metabolic engineering for vitamins production [J ] . Chinese Journal of Biotechnology , 2021 , 37 ( 5 ): 1748 - 1770 .

PONTRELLI S , CHIU T Y , LAN E I , et al . Escherichia coli as a host for metabolic engineering [J ] . Metabolic Engineering , 2018 , 50 : 16 - 46 .

RINALDI M A , FERRAZ C A , SCRUTTON N S . Alternative metabolic pathways and strategies to high-titre terpenoid production in Escherichia coli [J ] . Natural Product Reports , 2022 , 39 ( 1 ): 90 - 118 .

薛小莉 , 覃重军 . 大肠杆菌最小基因组分析和删减进展 [J ] . 生命科学 , 2013 , 25 ( 10 ): 978 - 982 .

XUE X L , QIN Z J . Progresses in the analysis and development of Escherichia coli minimal genome [J ] . Chinese Journal of Life Science , 2013 , 25 ( 10 ): 978 - 982 .

GAO H , QIU Z , WANG X , et al . Recent advances in genome-scale engineering in Escherichia coli and their applications [J ] . Engineering Microbiology , 2024 , 4 ( 1 ): 100115 .

JIANG M , ZHANG H . Engineering the shikimate pathway for biosynthesis of molecules with pharmaceutical activities in E. coli [J ] . Current Opinion in Biotechnology , 2016 , 42 : 1 - 6 .

XU W , YUAN J , YANG S , et al . Programming saposin-mediated compensatory metabolic sinks for enhancing ubiquinone productions [J ] . ACS Synthetic Biology , 2016 , 5 ( 12 ): 1404 - 1411 .

CLUIS C P , EKINS A , NARCROSS L , et al . Identification of bottlenecks in Escherichia coli engineered for the production of CoQ10 [J ] . Metabolic Engineering , 2011 , 13 ( 6 ): 733 - 744 .

LU Z , HUANG Z , WU Z , et al . High-level production of vitamin K2 in Escherichia coli via modular molecular engineering [J ] . Synthetic and Systems Biotechnology , 2026 , 12 : 42 - 51 .

LI Z , XU K , CHEN Y , et al . Rational design of heptaprenyl diphosphate synthase and metabolic optimization drive biosynthesis of menaquinone-7 by Escherichia coli [J ] . Bioresource Technology , 2026 , 135786 .

UBEROS H , AUGUSTIN M , LIÉBANA A , et al . Comparative study of the influence of melatonin and vitamin E on the surface characteristics of Escherichia coli [J ] . Letters in Applied Microbiology , 2001 , 32 ( 5 ): 303 - 306 .

ORSI E , BEEKWILDER J , EGGINK G , et al . The transition of Rhodobacter sphaeroides into a microbial cell factory [J ] . Biotechnology and Bioengineering , 2021 , 118 ( 2 ): 531 - 541 .

WANG J , KANG Q , CHEN X , et al . Systematic reprogramming of Rhodobacter sphaeroides for efficient biosynthesis of Coenzyme Q 10 and porphyrins [J ] . ACS Synthetic Biology , 2026 , 15 ( 6 ): 2196 - 2208 .

HE X , WANG D , LIU J , et al . Engineering the methylerythritol phosphate pathway and using a temporal promoter for enhanced lycopene production in Rhodobacter sphaeroides HY01 [J ] . Journal of Agricultural and Food Chemistry , 2024 , 72 ( 50 ): 28040 - 28047 .

ORSI E , BEEKWILDER J , VAN GELDER D , et al . Functional replacement of isoprenoid pathways in Rhodobacter sphaeroides [J ] . Microbial Biotechnology , 2020 , 13 ( 4 ): 1082 - 1093 .

CHEN H , WANG Y , WANG W , et al . High-yield porphyrin production through metabolic engineering and biocatalysis [J ] . Nature Biotechnology , 2025 , 43 ( 10 ): 1717 - 1727 .

ZHANG Y , SONG X , LAI Y , MO Q , YUAN J . High-yielding terpene-based biofuel production in Rhodobacter capsulatus [J ] . ACS Synthetic Biology , 2021 , 10 ( 6 ): 1545 - 1552 .

MOUGIAKOS I , ORSI E , GHIFFARY M R , et al . Efficient Cas9-based genome editing of Rhodobacter sphaeroides for metabolic engineering [J ] . Microbial Cell Factories , 2019 , 18 ( 1 ): 204 .

KOSTANJŠEK M , RAYNAL A , DIMOPOULOS G , et al . Rhodo-Box: A synthetic biology toolbox to facilitate metabolic engineering of Rhodobacter sphaeroides [J ] . ACS Synthetic Biology , 2026 , 15 ( 4 ): 1400 - 1411 .

BOTSTEIN D , FINK G R . Yeast: an experimental organism for 21st century biology [J ] . Genetics , 2011 , 189 ( 3 ): 695 - 704 .

江丽红 , 董昌 , 黄磊 , 等 . 酿酒酵母代谢工程技术 [J ] . 生物工程学报 , 2021 , 37 ( 5 ): 1578 - 1602 .

JIANG L H , DONG C , HUANG L , et al . Metabolic engineering tools for Saccharomyces cerevisiae [J ] . Chinese Journal of Biotechnology , 2021 , 37 ( 5 ): 1578 - 1602 .

陈明凯 , 叶丽丹 , 于洪巍 . 代谢改造酿酒酵母合成萜类化合物的研究进展 [J ] . 生物工程学报 , 2021 , 37 ( 6 ): 2085 - 2104 .

CHEN M K , YE L D , YU H W . Advances in metabolic engineering of Saccharomyces cerevisiae for terpenoids biosynthesis [J ] . Chinese Journal of Biotechnology , 2021 , 37 ( 6 ): 2085 - 2104 .

ZHANG Q , YU S , LYU Y , et al . Systematically engineered fatty acid catabolite pathway for the production of (2 S )-naringenin in Saccharomyces cerevisiae [J ] . ACS Synthetic Biology , 2021 , 10 ( 5 ): 1166 - 1175 .

SRINIVASAN P , SMOLKE C D . Biosynthesis of medicinal tropane alkaloids in yeast [J ] . Nature , 2020 , 585 ( 7826 ): 614 - 619 .

LIAN J , MISHRA S , ZHAO H . Recent advances in metabolic engineering of Saccharomyces cerevisiae : New tools and their applications [J ] . Metabolic Engineering , 2018 , 50 : 85 - 108 .

栾韬 , 尹梦琦 , 王明 , 康秀龙 , 赵建志 , 鲍晓明 . 酿酒酵母细胞器区室化合成化学品的研究进展 [J ] . 生物工程学报 , 2023 , 39 ( 6 ): 2334 - 2358 .

LUAN T , YIN M Q , WANG M , KANG X L , ZHAO J Z , BAO X M . Advances in the production of chemicals by organelle compartmentalization in Saccharomyces cerevisiae [J ] . Chinese Journal of Biotechnology , 2023 , 39 ( 6 ): 2334 - 2358 .

刘世昌 , 高教琪 , 周雍进 . 常见酵母的代谢工程研究进展 [J ] . 生物工程学报 , 2025 , 41 ( 11 ): 4037 - 4051 .

LIU S C , GAO J Q , ZHOU Y J . Advances in metabolic engineering of common yeast species [J ] . Chinese Journal of Biotechnology , 2025 , 41 ( 11 ): 4037 - 4051 .

FAN C , FAN J , CHEN H , et al . Switching the yeast metabolism via modulation of sugar phosphorylation [J ] . Metabolic Engineering , 2025 , 89 : 76 - 85 .

WANG Z , QI X , REN X , et al . Synthetic evolution of Saccharomyces cerevisiae for biomanufacturing: Approaches and applications [J ] . mLife , 2025 , 4 ( 1 ): 1 - 16 .

LEDESMA-AMARO R , NICAUD J M . Yarrowia lipolytica as a biotechnological chassis to produce usual and unusual fatty acids [J ] . Progress in Lipid Research , 2016 , 61 : 40 - 50 .

ABDEL-MAWGOUD A M , MARKHAM K A , PALMER C M , et al . Metabolic engineering in the host Yarrowia lipolytica [J ] . Metabolic Engineering , 2018 , 50 : 192 - 208 .

XU P , QIAO K , AHN W S , et al . Engineering Yarrowia lipolytica as a platform for synthesis of drop-in transportation fuels and oleochemicals [J ] . Proceedings of the National Academy of Sciences of the United States of America , 2016 , 113 ( 39 ): 10848 - 10853 .

NING Y , LIU M , RU Z , et al . Efficient synthesis of squalene by cytoplasmic-peroxisomal engineering and regulating lipid metabolism in Yarrowia lipolytica [J ] . Bioresource Technology , 2024 , 395 : 130379 .

LIU S C , XU L , SUN Y , et al . Sustainable high-titer production of β-elemene in Yarrowia lipolytica via modular engineering, pathway refactoring, and a chimeric germacrene A synthase [J ] . Chemical Engineering Journal , 2026 , 528 : 172276 .

SHANG Y , ZHANG P , WEI W , et al . Metabolic engineering for the high-yield production of polydatin in Yarrowia lipolytica [J ] . Bioresource Technology , 2023 , 381 : 129129 .

LIU H-H , JI X J , HUANG H . Biotechnological applications of Yarrowia lipolytica : Past, present and future [J ] . Biotechnology Advances , 2015 , 33 ( 8 ): 1522 - 1546 .

MILLER K K , ALPER H S . Yarrowia lipolytica : more than an oleaginous workhorse [J ] . Applied Microbiology and Biotechnology , 2019 , 103 ( 23 ): 9251 - 9262 .

KIKUCHI Y , TSUJIMOTO K , KURAHASHI O . Mutational analysis of the feedback sites of phenylalanine-sensitive 3-deoxy-D-arabino-heptulosonate-7-phosphate synthase of Escherichia coli [J ] . Applied and Environmental Microbiology , 1997 , 63 ( 2 ): 761 - 762 .

LÜTKE EVERSLOH T , STEPHANOPOULOS G . L -Tyrosine production by deregulated strains of Escherichia coli [J ] . Applied Microbiology and Biotechnology , 2007 , 75 ( 1 ): 103 - 110 .

GOLD N D , GOWEN C M , LUSSIER F X , et al . Metabolic engineering of a tyrosine-overproducing yeast platform using targeted metabolomics [J ] . Microbial Cell Factories , 2015 , 14 ( 1 ): 73 .

XU S , WANG Q , ZENG W , et al . Construction of a heat-inducible Escherichia coli strain for efficient de novo biosynthesis of L -tyrosine [J ] . Process Biochemistry , 2020 , 92 : 85 - 92 .

PING J , WANG L , QIN Z , ZHOU Z , ZHOU J . Synergetic engineering of Escherichia coli for efficient production of L -tyrosine [J ] . Synthetic and Systems Biotechnology , 2023 , 8 ( 4 ): 724 - 731 .

KOMA D , KISHIDA T , YOSHIDA E , et al . Chromosome engineering to generate plasmid-free phenylalanine- and tyrosine-overproducing Escherichia coli strains that can be applied in the generation of aromatic-compound-producing bacteria [J ] . Applied and Environmental Microbiology , 2020 , 86 ( 14 ).

BONGERS M , CHRYSANTHOPOULOS P K , BEHRENDORFF J B Y H , et al . Systems analysis of methylerythritol-phosphate pathway flux in E. coli : Insights into the role of oxidative stress and the validity of lycopene as an isoprenoid reporter metabolite [J ] . Microbial Cell Factories , 2015 , 14 ( 1 ): 193 .

XIE W , LV X , YE L , ZHOU P , YU H . Construction of lycopene-overproducing Saccharomyces cerevisiae by combining directed evolution and metabolic engineering [J ] . Metabolic Engineering , 2015 , 30 : 69 - 78 .

朱航志 , 蒋珊 , 陈丹 , 等 . 引入新型异戊二烯醇利用途径促进解脂耶氏酵母中β-胡萝卜素的合成 [J ] . 中国生物工程杂志 , 2021 , 41 ( 4 ): 37 - 46 .

ZHU H Z , JIANG S , CHEN D , et al . Improving the biosynthesis of β-carotene in yarrowia lipolytica by introducing an artificial isopentenol utilization pathway [J ] . China Biotechnology , 2021 , 41 ( 4 ): 37 - 46 .

KOCH M , LEMKE R , HEISE K P , MOCK H P . Characterization of γ-tocopherol methyltransferases from Capsicum annuum L and Arabidopsis thaliana [J ] . European Journal of Biochemistry , 2003 , 270 ( 1 ): 84 - 92 .

TEWARI K , DAHUJA A , SACHDEV A , et al . Molecular cloning, heterologous expression and functional characterization of gamma tocopherol me thyl transferase (γ-TMT) from Glycine max [J ] . Protein Expression and Purification , 2017 , 140 : 81 - 89 .

TAVVA V S , KIM Y H , KAGAN I A , et al . Increased α-tocopherol content in soybean seed overexpressing the Perilla frutescens γ-tocopherol methyltransferase gene [J ] . Plant Cell Reports , 2007 , 26 ( 1 ): 61 - 70 .

ZHANG L , LUO Y , LIU B , et al . Overexpression of the maize γ-tocopherol methyltransferase gene (ZmTMT) increases α-tocopherol content in transgenic Arabidopsis and maize seeds [J ] . Transgenic Research , 2020 , 29 ( 1 ): 95 - 104 .

SOMSRI A , CHU S H , NAWADE B , et al . Harnessing γ-TMT genetic variations and haplotypes for vitamin E diversity in the korean rice collection [J ] . Antioxidants , 2024 , 13 : 234 .

CHEN H , ZHENG F , FENG X , et al . Engineering archaeal membrane-spanning lipid GDGT biosynthesis in bacteria: Implications for early life membrane transformations [J ] . mLife , 2025 , 4 ( 2 ): 193 - 204 .

ADDLEE H A , HUNTER C N . Physical mapping and functional assignment of the geranylgeranyl-bacteriochlorophyll reductase gene, bchP, of Rhodobacter sphaeroides [J ] . Journal of Bacteriology , 1999 , 181 ( 23 ): 7248 - 7255 .

WALTHER T C , FARESE R V . Lipid droplets and cellular lipid metabolism [J ] . Annual Review of Biochemistry , 2012 , 81 : 687 - 714 .

BU X , LIN J Y , CHENG J , et al . Engineering endogenous ABC transporter with improving ATP supply and membrane flexibility enhances the secretion of β-carotene in Saccharomyces cerevisiae [J ] . Biotechnology for Biofuels , 2020 , 13 ( 1 ): 168 .

VERWAAL R , JIANG Y , WANG J , et al . Heterologous carotenoid production in Saccharomyces cerevisiae induces the pleiotropic drug resistance stress response [J ] . Yeast , 2010 , 27 ( 12 ): 983 - 998 .

MORAN G R . 4-Hydroxyphenylpyruvate dioxygenase [J ] . Archives of Biochemistry and Biophysics , 2005 , 433 ( 1 ): 117 - 128 .

MURAKAMI M , SHIBUYA K , NAKAYAMA T , et al . Geranylgeranyl reductase involved in the biosynthesis of archaeal membrane lipids in the hyperthermophilic archaeon Archaeoglobus fulgidus [J ] . The FEBS Journal , 2007 , 274 ( 3 ): 805 - 814 .

CUI G Z , MA Z , LIU Y J , et al . Overexpression of glucose-6-phosphate dehydrogenase enhanced the polyunsaturated fatty acid composition of Aurantiochytrium sp. SD116 [J ] . Algal Research , 2016 , 19 : 138 - 145 .

YUAN L , QIN Y L , ZOU Z C , et al . Enhancing intracellular NADPH bioavailability through improving pentose phosphate pathway flux and its application in biocatalysis asymmetric reduction reaction [J ] . Journal of Bioscience and Bioengineering , 2022 , 134 ( 6 ): 528 - 533 .

ZHAN M , KAN B , DONG J , et al . Metabolic engineering of Corynebacterium glutamicum for improved L -arginine synthesis by enhancing NADPH supply [J ] . Journal of Industrial Microbiology and Biotechnology , 2019 , 46 ( 1 ): 45 - 54 .

ZHANG Y , ZHOU Z , WU J , et al . Construction and application of an efficient methylation platform in Escherichia coli based on SAM regeneration [J ] . Chemical Engineering Journal , 2025 , 512 : 162642 .

MONTAÑO LÓPEZ J , DURAN L , AVALOS J L . Physiological limitations and opportunities in microbial metabolic engineering [J ] . Nature Reviews Microbiology , 2022 , 20 ( 1 ): 35 - 48 .

LIU L , DING D , WANG H , et al . Balancing cell growth and product synthesis for efficient microbial cell factories [J ] . Advanced Science , 2025 , 12 ( 40 ): e2510649 .

DIANAT M , STRAATEN S , MARITATO A , et al . Exploration of in situ extraction for enhanced triterpenoid production by Saccharomyces cerevisiae [J ] . Microbial Biotechnology , 2024 , 17 ( 12 ): e70061 .

COMBES J , IMATOUKENE N , COUVREUR J , et al . Intensification of p -coumaric acid heterologous production using extractive biphasic fermentation [J ] . Bioresource Technology , 2021 , 337 : 125436 .