LUCIDO A , BASALLO O , MARIN-SANGUINO A , et al . Multiscale mathematical modeling in systems biology: a framework to boost plant synthetic biology [J ] . Plants , 2025 , 14 ( 3 ): 470 .

KE J , WANG B , YOSHIKUNI Y . Microbiome engineering: synthetic biology of plant-associated microbiomes in sustainable agriculture [J ] . Trends in Biotechnology , 2021 , 39 ( 3 ): 244 - 261 .

张博 , 马永硕 , 尚轶 , 等 . 植物合成生物学研究进展 [J ] . 合成生物学 , 2020 , 1 ( 2 ): 121 - 140 .

ZHANG B , MA Y S , SHANG Y , et al . Recent advances in plant synthetic biology [J ] . Synthetic Biology Journal , 2020 , 1 ( 2 ): 121 - 140 .

BROPHY J A N , MAGALLON K J , DUAN L N , et al . Synthetic genetic circuits as a means of reprogramming plant roots [J ] . Science , 2022 , 377 ( 6607 ): 747 - 751 .

ZHANG X N , LIU C L , DAI J B , et al . Enabling technology and core theory of synthetic biology [J ] . Science China Life Sciences , 2023 , 66 ( 8 ): 1742 - 1785 .

DOUDNA J A , CHARPENTIER E . The new frontier of genome engineering with CRISPR-Cas9 [J ] . Science , 2014 , 346 ( 6213 ): 1258096 .

TIAN Y S , WANG B , PENG R H , et al . Enhancing carotenoid biosynthesis in rice endosperm by metabolic engineering [J ] . Plant Biotechnology Journal , 2019 , 17 ( 5 ): 849 - 851 .

XIONG D L . Perspectives of improving rice photosynthesis for higher grain yield [J ] . Crop and Environment , 2024 , 3 ( 3 ): 123 - 137 .

燕永亮 , 田长富 , 杨建国 , 等 . 人工高效生物固氮体系创建及其农业应用 [J ] . 生命科学 , 2021 , 33 ( 12 ): 1532 - 1543 .

YAN Y L , TIAN C F , YANG J G , et al . Establishment of artificial efficiency biological nitrogen fixation system and its agricultural application [J ] . Chinese Bulletin of Life Sciences , 2021 , 33 ( 12 ): 1532 - 1543 .

潘明慧 , 杨雪 , 杜国忠 , 等 . 合成生物学助力链霉菌天然产物农药创制与产业化 [J ] . 植物保护 , 2023 , 49 ( 5 ): 371 - 389 .

PAN M H , YANG X , DU G Z , et al . The exploitation and bio-manufacture of natural product pesticides from Streptomyces by synthetic biology [J ] . Plant Protection , 2023 , 49 ( 5 ): 371 - 389 .

GARDNER T S , CANTOR C R , COLLINS J J . Construction of a genetic toggle switch in Escherichia coli [J ] . Nature , 2000 , 403 ( 6767 ): 339 - 342 .

YE X , AL-BABILI S , KLÖTI A , et al . Engineering the provitamin A (beta-carotene) biosynthetic pathway into (carotenoid-free) rice endosperm [J ] . Science , 2000 , 287 ( 5451 ): 303 - 305 .

BHULLAR S , CHAKRAVARTHY S , ADVANI S , et al . Strategies for development of functionally equivalent promoters with minimum sequence homology for transgene expression in plants: cis-elements in a novel DNA context versus domain swapping [J ] . Plant Physiology , 2003 , 132 ( 2 ): 988 - 998 .

ENGLER C , KANDZIA R , MARILLONNET S . A one pot, one step, precision cloning method with high throughput capability [J ] . PLoS One , 2008 , 3 ( 11 ): e3647 .

ENGLER C , GRUETZNER R , KANDZIA R , et al . Golden Gate shuffling: a one-pot DNA shuffling method based on type IIs restriction enzymes [J ] . PLoS One , 2009 , 4 ( 5 ): e5553 .

AMOS M . Population-based microbial computing: a third wave of synthetic biology? [J ] . International Journal of General Systems , 2014 , 43 ( 7 ): 770 - 782 .

FISHER A K , FREEDMAN B G , BEVAN D R , et al . A review of metabolic and enzymatic engineering strategies for designing and optimizing performance of microbial cell factories [J ] . Computational and Structural Biotechnology Journal , 2014 , 11 ( 18 ): 91 - 99 .

LU X F , VORA H , KHOSLA C . Overproduction of free fatty acids in E . coli : implications for biodiesel production [J ] . Metabolic Engineering , 2008 , 10 ( 6 ): 333 - 339 .

CONG L , RAN F A , COX D , et al . Multiplex genome engineering using CRISPR/Cas systems [J ] . Science , 2013 , 339 ( 6121 ): 819 - 823 .

SHAN Q W , WANG Y P , LI J , et al . Targeted genome modification of crop plants using a CRISPR-Cas system [J ] . Nature Biotechnology , 2013 , 31 ( 8 ): 686 - 688 .

CHAN A N , WANG L L , ZHU Y J , et al . Identification through fine mapping and verification using CRISPR/Cas9-targeted mutagenesis for a minor QTL controlling grain weight in rice [J ] . Theoretical and Applied Genetics , 2021 , 134 ( 1 ): 327 - 337 .

JARVIS D E , HO Y S , LIGHTFOOT D J , et al . The genome of Chenopodium quinoa [J ] . Nature , 2017 , 542 ( 7641 ): 307 - 312 .

ZIMIN A V , PUIU D , HALL R , et al . The first near-complete assembly of the hexaploid bread wheat genome, Triticum aestivum [J ] . GigaScience , 2017 , 6 ( 11 ): 1 - 7 .

YU W C , YAU Y Y , BIRCHLER J A . Plant artificial chromosome technology and its potential application in genetic engineering [J ] . Plant Biotechnology Journal , 2016 , 14 ( 5 ): 1175 - 1182 .

XU C H , BIRCHLER J A . Editorial: Plant artificial chromosomes: progress and perspectives [J ] . Frontiers in Plant Science , 2023 , 14 : 1290386 .

RAVIKUMAR S , BAYLON M G , PARK S J , et al . Engineered microbial biosensors based on bacterial two-component systems as synthetic biotechnology platforms in bioremediation and biorefinery [J ] . Microbial Cell Factories , 2017 , 16 ( 1 ): 62 .

ARACIC S , MANNA S , PETROVSKI S , et al . Innovative biological approaches for monitoring and improving water quality [J ] . Frontiers in Microbiology , 2015 , 6 : 826 .

NEETHIRAJAN S , RAGAVAN V , WENG X , et al . Biosensors for sustainable food engineering: challenges and perspectives [J ] . Biosensors , 2018 , 8 ( 1 ): 23 .

刘华梅 , 许国建 . 微生物农药苏云金杆菌G033A [J ] . 农药科学与管理 , 2018 , 39 ( 4 ): 59 - 60 .

LIU H M , XU G J . Microbial pesticide Bacillus thuringiensis G033A [J ] . Pesticide Science and Administration , 2018 , 39 ( 4 ): 59 - 60 .

王晓梅 , 李辉尚 , 杨小薇 . 全球农业合成生物学发展现状及对中国的启示 [J ] . 农业展望 , 2023 , 19 ( 4 ): 71 - 76 .

WANG X M , LI H S , YANG X W . Development status of global agricultural synthetic biology and its enlightenment to China [J ] . Agricultural Outlook , 2023 , 19 ( 4 ): 71 - 76 .

WALTZ E . GABA-enriched tomato is first CRISPR-edited food to enter market [J ] . Nature Biotechnology , 2022 , 40 ( 1 ): 9 - 11 .

PAINE J A , SHIPTON C A , CHAGGAR S , et al . Improving the nutritional value of Golden Rice through increased pro-vitamin A content [J ] . Nature Biotechnology , 2005 , 23 ( 4 ): 482 - 487 .

ANTUNES M S , MOREY K J , SMITH J J , et al . Programmable ligand detection system in plants through a synthetic signal transduction pathway [J ] . PLoS One , 2011 , 6 ( 1 ): e16292 .

BULLEY S , WRIGHT M , ROMMENS C , et al . Enhancing ascorbate in fruits and tubers through over-expression of the L-galactose pathway gene GDP-L-galactose phosphorylase [J ] . Plant Biotechnology Journal , 2012 , 10 ( 4 ): 390 - 397 .

ROGERS C , OLDROYD G E D . Synthetic biology approaches to engineering the nitrogen symbiosis in cereals [J ] . Journal of Experimental Botany , 2014 , 65 ( 8 ): 1939 - 1946 .

RUIZ-LOPEZ N , HASLAM R P , NAPIER J A , et al . Successful high-level accumulation of fish oil omega-3 long-chain polyunsaturated fatty acids in a transgenic oilseed crop [J ] . The Plant Journal , 2014 , 77 ( 2 ): 198 - 208 .

WANG B , KASHKOOLI A B , SALLETS A , et al . Transient production of artemisinin in Nicotiana benthamiana is boosted by a specific lipid transfer protein from A. annua [J ] . Metabolic Engineering , 2016 , 38 : 159 - 169 .

NONAKA S , ARAI C , TAKAYAMA M , et al . Efficient increase of γ-aminobutyric acid (GABA) content in tomato fruits by targeted mutagenesis [J ] . Scientific Reports , 2017 , 7 : 7057 .

PERCHLIK M , TEGEDER M . Improving plant nitrogen use efficiency through alteration of amino acid transport processes [J ] . Plant Physiology , 2017 , 175 ( 1 ): 235 - 247 .

ZHU Q L , ZENG D C , YU S Z , et al . From golden rice to aSTARice: bioengineering astaxanthin biosynthesis in rice endosperm [J ] . Molecular Plant , 2018 , 11 ( 12 ): 1440 - 1448 .

SOUTH P F , CAVANAGH A P , LIU H W , et al . Synthetic glycolate metabolism pathways stimulate crop growth and productivity in the field [J ] . Science , 2019 , 363 ( 6422 ): eaat9077 .

SHEN B R , WANG L M , LIN X L , et al . Engineering a new chloroplastic photorespiratory bypass to increase photosynthetic efficiency and productivity in rice [J ] . Molecular Plant , 2019 , 12 ( 2 ): 199 - 214 .

LIN Q P , ZONG Y , XUE C X , et al . Prime genome editing in rice and wheat [J ] . Nature Biotechnology , 2020 , 38 ( 5 ): 582 - 585 .

ROELL M S , SCHADA VON BORZYKOWSKI L , WESTHOFF P , et al . A synthetic C 4 shuttle via the β-hydroxyaspartate cycle in C 3 plants [J ] . Proceedings of the National Academy of Sciences of the United States of America , 2021 , 118 ( 21 ): e2022307118 .

HONG B K , GRZECH D , CAPUTI L , et al . Biosynthesis of strychnine [J ] . Nature , 2022 , 607 ( 7919 ): 617 - 622 .

MARTIN L B B , KIKUCHI S , REJZEK M , et al . Complete biosynthesis of the potent vaccine adjuvant QS-21 [J ] . Nature Chemical Biology , 2024 , 20 ( 4 ): 493 - 502 .

WU H X , YANG J H , SHEN P J , et al . High-level production of indole-3-acetic acid in the metabolically engineered Escherichia coli [J ] . Journal of Agricultural and Food Chemistry , 2021 , 69 ( 6 ): 1916 - 1924 .

ZHANG Y , CHIU T Y , ZHANG J T , et al . Systematical engineering of synthetic yeast for enhanced production of lycopene [J ] . Bioengineering , 2021 , 8 ( 1 ): 14 .

ZHANG Y , YUAN M D , WU X X , et al . The construction and optimization of engineered yeast chassis for efficient biosynthesis of 8-hydroxygeraniol [J ] . mLife , 2023 , 2 ( 4 ): 438 - 449 .

ZHU Y , LI J X , PENG L Y , et al . High-yield production of protopanaxadiol from sugarcane molasses by metabolically engineered Saccharomyces cerevisiae [J ] . Microbial Cell Factories , 2022 , 21 ( 1 ): 230 .

TAN G Y , DENG K H , LIU X H , et al . Heterologous biosynthesis of spinosad: an omics-guided large polyketide synthase gene cluster reconstitution in Streptomyces [J ] . ACS Synthetic Biology , 2017 , 6 ( 6 ): 995 - 1005 .

WANG Q , BAO T , HU M K , et al . Efficient acetoin production in Bacillus subtilis by multivariate modular metabolic engineering with spatiotemporal modulation [J ] . ACS Sustainable Chemistry & Engineering , 2025 , 13 ( 5 ): 1927 - 1936 .

SABATE R , DE GROOT N S , VENTURA S . Protein folding and aggregation in bacteria [J ] . Cellular and Molecular Life Sciences , 2010 , 67 ( 16 ): 2695 - 2715 .

TIAN Y , KONG L Z , LI Q , et al . Structural diversity, evolutionary origin, and metabolic engineering of plant specialized benzylisoquinoline alkaloids [J ] . Natural Product Reports , 2024 , 41 ( 11 ): 1787 - 1810 .

SUN Q Y , DING L W , LOMONOSSOFF G P , et al . Improved expression and purification of recombinant human serum albumin from transgenic tobacco suspension culture [J ] . Journal of Biotechnology , 2011 , 155 ( 2 ): 164 - 172 .

VASILEV N , SCHMITZ C , DONG L M , et al . Comparison of plant-based expression platforms for the heterologous production of geraniol [J ] . Plant Cell, Tissue and Organ Culture (PCTOC) , 2014 , 117 ( 3 ): 373 - 380 .

YOUSEFIAN S , LOHRASEBI T , FARHADPOUR M , et al . Effect of methyl jasmonate on phenolic acids accumulation and the expression profile of their biosynthesis-related genes in Mentha spicata hairy root cu ltures [J ] . Plant Cell, Tissue and Organ Culture (PCTOC) , 2020 , 142 ( 2 ): 285 - 297 .

WU S J , XIE X G , FENG K M , et al . Transcriptome sequencing and signal transduction for the enhanced tanshinone production in Salvia miltiorrhiza hairy roots induced by Trichoderma atroviride D16 polysaccharide fraction [J ] . Bioscience, Biotechnology, and Biochemistry , 2022 , 86 ( 8 ): 1049 - 1059 .

KUŹMA Ł , KISIEL W , KRÓLICKA A , et al . Genetic transformation of Salvia austriaca by Agrobacterium rhizogenes and diterpenoid isolation [J ] . Die Pharmazie , 2011 , 66 ( 11 ): 904 - 907 .

LIU J , ZHAO Y X , ZHANG J M , et al . Production of species-specific anthocyanins through an inducible system in plant hairy roots [J ] . Metabolic Engineering , 2024 , 81 : 182 - 196 .

NOGUEIRA M , ENFISSI E M A , WELSCH R , et al . Construction of a fusion enzyme for astaxanthin formation and its characterisation in microbial and plant hosts: a new tool for engineering ketocarotenoids [J ] . Metabolic Engineering , 2019 , 52 : 243 - 252 .

BEYRAGHDAR KASHKOOLI A , VAN DER KROL A R , RABE P , et al . Substrate promiscuity of enzymes from the sesquiterpene biosynthetic pathways from Artemisia annua and Tanacetum parthenium allows for novel combinatorial sesquiterpene production [J ] . Metabolic Engineering , 2019 , 54 : 12 - 23 .

FORESTIER E C F , CZECHOWSKI T , CORDING A C , et al . Developing a Nicotiana benthamiana transgenic platform for high-value diterpene production and candidate gene evaluation [J ] . Plant Biotechnology Journal , 2021 , 19 ( 8 ): 1614 - 1623 .

ZHU Q L , YU S Z , ZENG D C , et al . Development of “purple endosperm rice” by engineering anthocyanin biosynthesis in the endosperm with a high-efficiency transgene stacking system [J ] . Molecular Plant , 2017 , 10 ( 7 ): 918 - 929 .

LIU X Q , MA X H , WANG H , et al . Metabolic engineering of astaxanthin-rich maize and its use in the production of biofortified eggs [J ] . Plant Biotechnology Journal , 2021 , 19 ( 9 ): 1812 - 1823 .

LAM E , MICHAEL T P . Wolffia , a minimalist plant and synthetic biology chassis [J ] . Trends in Plant Science , 2022 , 27 ( 5 ): 430 - 439 .

BI G Q , ZHAO S J , YAO J W , et al . Near telomere-to-telomere genome of the model plant Physcomitrium patens [J ] . Nature Plants , 2024 , 10 ( 2 ): 327 - 343 .

LI Y Z , LIANG J , DENG B F , et al . Applications and prospects of CRISPR/Cas9-mediated base editing in plant breeding [J ] . Current Issues in Molecular Biology , 2023 , 45 ( 2 ): 918 - 935 .

GAUDELLI N M , KOMOR A C , REES H A , et al . Programmable base editing of A•T to G•C in genomic DNA without DNA cleavage [J ] . Nature , 2017 , 551 ( 7681 ): 464 - 471 .

LI C , ZHANG R , MENG X B , et al . Targeted, random mutagenesis of plant genes with dual cytosine and adenine base editors [J ] . Nature Biotechnology , 2020 , 38 ( 7 ): 875 - 882 .

MIKAMI M , TOKI S , ENDO M . In planta processing of the Sp Cas9-gRNA complex [J ] . Plant & Cell Physiology , 2017 , 58 ( 11 ): 1857 - 1867 .

CAMPA C C , WEISBACH N R , SANTINHA A J , et al . Multiplexed genome engineering by Cas12a and CRISPR arrays encoded on single transcripts [J ] . Nature Methods , 2019 , 16 ( 9 ): 887 - 893 .

LI C , ZONG Y , JIN S , et al . SWISS: multiplexed orthogonal genome editing in plants with a Cas9 nickase and engineered CRISPR RNA scaffolds [J ] . Genome Biology , 2020 , 21 ( 1 ): 141 .

LIU H J , JIAN L M , XU J T , et al . High-throughput CRISPR/Cas9 mutagenesis streamlines trait gene identification in maize [J ] . The Plant Cell , 2020 , 32 ( 5 ): 1397 - 1413 .

BAI M Y , YUAN J H , KUANG H Q , et al . Generation of a multiplex mutagenesis population via pooled CRISPR-Cas9 in soya bean [J ] . Plant Biotechnology Journal , 2020 , 18 ( 3 ): 721 - 731 .

JACOBS T B , ZHANG N , PATEL D , et al . Generation of a collection of mutant tomato lines using pooled CRISPR libraries [J ] . Plant Physiology , 2017 , 174 ( 4 ): 2023 - 2037 .

KUANG Y J , LI S F , REN B , et al . Base-editing-mediated artificial evolution of OsALS1 in planta to develop novel herbicide-tolerant rice germplasms [J ] . Molecular Plant , 2020 , 13 ( 4 ): 565 - 572 .

HU N , TIAN H L , LI Y H , et al . pHNRhCas9NG, single expression cassette-based dual-component dual-transcription unit CRISPR/Cas9 system for plant genome editing [J ] . Trends in Biotechnology , 2025 , 43 ( 7 ): 1788 - 1808 .

TATSIS E C , O’CONNOR S E . New developments in engineering plant metabolic pathways [J ] . Current Opinion in Biotechnology , 2016 , 42 : 126 - 132 .

WU Q Y , HUANG Z Y , WANG J Y , et al . Construction of an Escherichia coli cell factory to synthesize taxadien-5α-ol, the key precursor of anti-cancer drug paclitaxel [J ] . Bioresources and Bioprocessing , 2022 , 9 ( 1 ): 82 .

IGNEA C , ATHANASAKOGLOU A , IOANNOU E , et al . Carnosic acid biosynthesis elucidated by a synthetic biology platform [J ] . Proceedings of the National Academy of Sciences of the United States of America , 2016 , 113 ( 13 ): 3681 - 3686 .

TAN H X , CHEN X H , LIANG N , et al . Transcriptome analysis reveals novel enzymes for apo-carotenoid biosynthesis in saffron and allows construction of a pathway for crocetin synthesis in yeast [J ] . Journal of Experimental Botany , 2019 , 70 ( 18 ): 4819 - 4834 .

ZHANG J , HANSEN L G , GUDICH O , et al . A microbial supply chain for production of the anti-cancer drug vinblastine [J ] . Nature , 2022 , 609 ( 7926 ): 341 - 347 .

LIU J Q , WANG X , DAI G Z , et al . Microbial chassis engineering drives heterologous production of complex secondary metabolites [J ] . Biotechnology Advances , 2022 , 59 : 107966 .

KWAN B D , SELIGMANN B , NGUYEN T D , et al . Leveraging synthetic biology and metabolic engineering to overcome obstacles in plant pathway elucidation [J ] . Current Opinion in Plant Biology , 2023 , 71 : 102330 .

BUTELLI E , TITTA L , GIORGIO M , et al . Enrichment of tomato fruit with health-promoting anthocyanins by expression of select transcription factors [J ] . Nature Biotechnology , 2008 , 26 ( 11 ): 1301 - 1308 .

MALHOTRA K , SUBRAMANIYAN M , RAWAT K , et al . Compartmentalized metabolic engineering for artemisinin biosynthesis and effective malaria treatment by oral delivery of plant cells [J ] . Molecular Plant , 2016 , 9 ( 11 ): 1464 - 1477 .

POLTURAK G , BREITEL D , GROSSMAN N , et al . Elucidation of the first committed step in betalain biosynthesis enables the heterologous engineering of betalain pigments in plants [J ] . New Phytologist , 2016 , 210 ( 1 ): 269 - 283 .

MAJER E , LLORENTE B , RODRÍGUEZ-CONCEPCIÓN M , et al . Rewiring carotenoid biosynthesis in plants using a viral vector [J ] . Scientific Reports , 2017 , 7 : 41645 .

FUCHS L K , HOLLAND A H , LUDLOW R A , et al . Genetic manipulation of biosynthetic pathways in mint [J ] . Frontiers in Plant Science , 2022 , 13 : 928178 .

D’ANDREA L , SIMON-MOYA M , LLORENTE B , et al . Interference with Clp protease impairs carotenoid accumulation during tomato fruit ripening [J ] . Journal of Experimental Botany , 2018 , 69 ( 7 ): 1557 - 1568 .

PETRIE J R , SHRESTHA P , BELIDE S , et al . Metabolic engineering Camelina sativa with fish oil-like levels of DHA [J ] . PLoS One , 2014 , 9 ( 1 ): e85061 .

KIM J Y , KIM J H , JANG Y H , et al . Transcriptome and metabolite profiling of tomato SGR-knockout null lines using the CRISPR/Cas9 system [J ] . International Journal of Molecular Sciences , 2023 , 24 ( 1 ): 109 .

TU M X , FANG J H , ZHAO R K , et al . CRISPR/Cas9-mediated mutagenesis of VvbZIP36 promotes anthocyanin accumulation in grapevine ( Vitis vinifera ) [J ] . Horticulture Research , 2022 , 9 : uhac022 .

WEN D , WU L , WANG M Y , et al . CRISPR/Cas9-mediated targeted mutagenesis of FtMYB45 promotes flavonoid biosynthesis in Tartary buckwheat ( Fagopyrum tataricum ) [J ] . Frontiers in Plant Science , 2022 , 13 : 879390 .

DEVIREDDY A R , ZANDALINAS S I , FICHMAN Y , et al . Integration of reactive oxygen species and hormone signaling during abiotic stress [J ] . The Plant Journal , 2021 , 105 ( 2 ): 459 - 476 .

MIYAWAKI A , LLOPIS J , HEIM R , et al . Fluorescent indicators for Ca 2+ based on green fluorescent proteins and calmodulin [J ] . Nature , 1997 , 388 ( 6645 ): 882 - 887 .

BRUNOUD G , WELLS D M , OLIVA M , et al . A novel sensor to map auxin response and distribution at high spatio-temporal resolution [J ] . Nature , 2012 , 482 ( 7383 ): 103 - 106 .

LIAO C Y , SMET W , BRUNOUD G , et al . Reporters for sensitive and quantitative measurement of auxin response [J ] . Nature Methods , 2015 , 12 ( 3 ): 207 - 210 .

JONES A M . A new look at stress: abscisic acid patterns and dynamics at high-resolution [J ] . New Phytologist , 2016 , 210 ( 1 ): 38 - 44 .

FERNANDEZ-MORENO J P , STEPANOVA A N . Monitoring ethylene in plants: genetically encoded reporters and biosensors [J ] . Small Methods , 2020 , 4 ( 8 ): 1900260 .

RIZZA A , JONES A M . The makings of a gradient: spatiotemporal distribution of gibberellins in plant development [J ] . Current Opinion in Plant Biology , 2019 , 47 : 9 - 15 .

ORTEGA-VILLASANTE C , BURÉN S , BARÓN-SOLA Á , et al . In vivo ROS and redox potential fluorescent detection in plants: present approaches and future perspectives [J ] . Methods , 2016 , 109 : 92 - 104 .

ZÜRCHER E , TAVOR-DESLEX D , LITUIEV D , et al . A robust and sensitive synthetic sensor to monitor the transcriptional output of the cytokinin signaling network in planta [J ] . Plant Physiology , 2013 , 161 ( 3 ): 1066 - 1075 .

STEINER E , LIVNE S , KOBINSON-KATZ T , et al . The putative O-linked N -acetylglucosamine transferase SPINDLY inhibits class Ⅰ TCP proteolysis to promote sensitivity to cytokinin [J ] . Plant Physiology , 2016 , 171 ( 2 ): 1485 - 1494 .

WU R , DUAN L N , PRUNEDA-PAZ J L , et al . The 6xABRE synthetic promoter enables the spatiotemporal analysis of ABA-mediated transcriptional regulation [J ] . Plant Physiology , 2018 , 177 ( 4 ): 1650 - 1665 .

MÜLLER B , SHEEN J . Cytokinin and auxin interaction in root stem-cell specification during early embryogenesis [J ] . Nature , 2008 , 453 ( 7198 ): 1094 - 1097 .

RIZZA A , WALIA A , TANG B J , et al . Visualizing cellular gibberellin levels using the nlsGPS1 Förster resonance energy transfer (FRET) biosensor [J ] . Journal of Visualized Experiments , 2019 ( 143 ): e58739 .

LARRIEU A , CHAMPION A , LEGRAND J , et al . A fluorescent hormone biosensor reveals the dynamics of jasmonate signalling in plants [J ] . Nature Communications , 2015 , 6 : 6043 .

POUVREAU B , VANHERCKE T , SINGH S . From plant metabolic engineering to plant synthetic biology: the evolution of the design/build/test/learn cycle [J ] . Plant Science , 2018 , 273 : 3 - 12 .

WONG M H , GIRALDO J P , KWAK S Y , et al . Nitroaromatic detection and infrared communication from wild-type plants using plant nanobionics [J ] . Nature Materials , 2017 , 16 ( 2 ): 264 - 272 .

PENG Y H , ALLEN S , MILLWOOD R J , et al . ‘Fukusensor’: a genetically engineered plant for reporting DNA damage in response to gamma radiation [J ] . Plant Biotechnology Journal , 2014 , 12 ( 9 ): 1329 - 1332 .

JEZ J M , LEE S G , SHERP A M . The next green movement: Plant biology for the environment and sustainability [J ] . Science , 2016 , 353 ( 6305 ): 1241 - 1244 .

FICHMAN Y , MILLER G , MITTLER R . Whole-plant live imaging of reactive oxygen species [J ] . Molecular Plant , 2019 , 12 ( 9 ): 1203 - 1210 .

MCADAM E L , REID J B , FOO E . Gibberellins promote nodule organogenesis but inhibit the infection stages of nodulation [J ] . Journal of Experimental Botany , 2018 , 69 ( 8 ): 2117 - 2130 .

CRUZ A P Z , FERREIRA V , PIANZZOLA M J , et al . A novel, sensitive method to evaluate potato germplasm for bacterial wilt resistance using a luminescent Ralstonia solanacearum reporter strain [J ] . Molecular Plant-Microbe Interactions , 2014 , 27 ( 3 ): 277 - 285 .

MOHAMMAD-RAZDARI A , ROUSSEAU D , BAKHSHIPOUR A , et al . Recent advances in E-monitoring of plant diseases [J ] . Biosensors and Bioelectronics , 2022 , 201 : 113953 .

JUGDER B E , ERTAN H , BOHL S , et al . Organohalide respiring bacteria and reductive dehalogenases: key tools in organohalide bioremediation [J ] . Frontiers in Microbiology , 2016 , 7 : 249 .

SAHA G , SHAHRIN F , KHAN F H , et al . Smart IoT-driven precision agriculture: land mapping, crop prediction, and irrigation system [J ] . PLoS One , 2025 , 20 ( 3 ): e0319268 .

CUZICK A , MAGUIRE K , HAMMOND-KOSACK K E . Lack of the plant signalling component SGT1b enhances disease resistance to Fusarium culmorum in Arabidopsis buds and flowers [J ] . New Phytologist , 2009 , 181 ( 4 ): 901 - 912 .

JEONG H J , JUNG K H . Rice tissue-specific promoters and condition-dependent promoters for effective translational application [J ] . Journal of Integrative Plant Biology , 2015 , 57 ( 11 ): 913 - 924 .

JUSIAK B , CLETO S , PEREZ-PIÑERA P , et al . Engineering synthetic gene circuits in living cells with CRISPR technology [J ] . Trends in Biotechnology , 2016 , 34 ( 7 ): 535 - 547 .

LLOYD J P B , LY F , GONG P , et al . Synthetic memory circuits for stable cell reprogramming in plants [J ] . Nature Biotechnology , 2022 , 40 ( 12 ): 1862 - 1872 .

KHAN M A , HERRING G , ZHU J Y , et al . CRISPRi-based circuits to control gene expression in plants [J ] . Nature Biotechnology , 2025 , 43 ( 3 ): 416 - 430 .

WEINBERG B H , HANG PHAM N T , CARABALLO L D , et al . Large-scale design of robust genetic circuits with multiple inputs and outputs for mammalian cells [J ] . Nature Biotechnology , 2017 , 35 ( 5 ): 453 - 462 .

GUIZIOU S , MARANAS C J , CHU J C , et al . An integrase toolbox to record gene-expression during plant development [J ] . Nature Communications , 2023 , 14 : 1844 .

LI S S , LI Z L , TAN G Y , et al . In vitro allosteric transcription factor-based biosensing [J ] . Trends in Biotechnology , 2023 , 41 ( 8 ): 1080 - 1095 .

FERREIRA S S , ANTUNES M S . Genetically encoded Boolean logic operators to sense and integrate phenylpropanoid metabolite levels in plants [J ] . New Phytologist , 2024 , 243 ( 2 ): 674 - 687 .

LIANG Y , RICHARDSON S , YAN J W , et al . Endoribonuclease-based two-component repressor systems for tight gene expression control in plants [J ] . ACS Synthetic Biology , 2017 , 6 ( 5 ): 806 - 816 .

KASENIIT K E , KATZ N , KOLBER N S , et al . Modular, programmable RNA sensing using ADAR editing in living cells [J ] . Nature Biotechnology , 2023 , 41 ( 4 ): 482 - 487 .

KONERMANN S , LOTFY P , BRIDEAU N J , et al . Transcriptome engineering with RNA-targeting type Ⅵ-D CRISPR effectors [J ] . Cell , 2018 , 173 ( 3 ): 665 - 676.e14 .

MARAND A P , CHEN Z L , GALLAVOTTI A , et al . A cis -regulatory atlas in maize at single-cell resolution [J ] . Cell , 2021 , 184 ( 11 ): 3041 - 3055.e21 .

HE Z H , LUO Y T , ZHOU X K , et al . scPlantDB: a comprehensive database for exploring cell types and markers of plant cell atlases [J ] . Nucleic Acids Research , 2024 , 52 ( D1 ): D1629 - D1638 .

RAI K , WANG Y D , O’CONNELL R W , et al . Using machine learning to enhance and accelerate synthetic biology [J ] . Current Opinion in Biomedical Engineering , 2024 , 31 : 100553 .

LAM H Y I , ONG X E , MUTWIL M . Large language models in plant biology [J ] . Trends in Plant Science , 2024 , 29 ( 10 ): 1145 - 1155 .

ZHU W C , HAN R , SHANG X Y , et al . The CropGPT project: Call for a global, coordinated effort in precision design breeding driven by AI using biological big data [J ] . Molecular Plant , 2024 , 17 ( 2 ): 215 - 218 .

JI Y R , ZHOU Z H , LIU H , et al . DNABERT: pre-trained Bidirectional Encoder Representations from Transformers model for DNA-language in genome [J ] . Bioinformatics , 2021 , 37 ( 15 ): 2112 - 2120 .

ABRAMSON J , ADLER J , DUNGER J , et al . Addendum: accurate structure prediction of biomolecular interactions with AlphaFold 3 [J ] . Nature , 2024 , 636 ( 8042 ): E4 .

CUI H T , WANG C , MAAN H , et al . scGPT: toward building a foundation model for single-cell multi-omics using generative AI [J ] . Nature Methods , 2024 , 21 ( 8 ): 1470 - 1480 .

WANG J , ZHANG L , WANG S D , et al . AlphaFold-guided bespoke gene editing enhances field-grown soybean oil contents [J ] . Advanced Science , 2025 , 12 ( 23 ): 2500290 .

宋成治 , 林一瀚 . AI+定向进化赋能蛋白改造及优化 [J ] . 合成生物学 , 2025 , 6 ( 3 ): 617 - 635 .

SONG C Z , LIN Y H . AI-enabled directed evolution for protein engineering and optimization [J ] . Synthetic Biology Journal , 2025 , 6 ( 3 ): 617 - 635 .

SHARMA A , JAIN A , GUPTA P , et al . Machine learning applications for precision agriculture: a comprehensive review [J ] . IEEE Access , 2021 , 9 : 4843 - 4873 .

LIAKOS K G , BUSATO P , MOSHOU D , et al . Machine learning in agriculture: a review [J ] . Sensors , 2018 , 18 ( 8 ): 2674 .

ESLAMI M , ADLER A , CACERES R S , et al . Artificial intelligence for synthetic biology [J ] . Communications of the ACM , 2022 , 65 ( 5 ): 88 - 97 .

YANG J S , REYNA-LLORENS I . Plant synthetic biology: exploring the frontiers of sustainable agriculture and fundamental plant biology [J ] . Journal of Experimental Botany , 2023 , 74 ( 13 ): 3787 - 3790 .

ORR D J , ALCÂNTARA A , KAPRALOV M V , et al . Surveying Rubisco diversity and temperature response to improve crop photosynthetic efficiency [J ] . Plant Physiology , 2016 , 172 ( 2 ): 707 - 717 .

LIN M T , OCCHIALINI A , ANDRALOJC P J , et al . A faster Rubisco with potential to increase photosynthesis in crops [J ] . Nature , 2014 , 513 ( 7519 ): 547 - 550 .

MANNING T , BIRCH R , STEVENSON T , et al . Bacterial Form Ⅱ Rubisco can support wild-type growth and productivity in Solanum tuberosum cv. Desiree (potato) under elevated CO 2 [J ] . PNAS Nexus , 2023 , 2 ( 2 ): pgac305 .

GUNN L H , MARTIN AVILA E , BIRCH R , et al . The dependency of red Rubisco on its cognate activase for enhancing plant photosynthesis and growth [J ] . Proceedings of the National Academy of Sciences of the United States of America , 2020 , 117 ( 41 ): 25890 - 25896 .

MATSUMURA H , SHIOMI K , YAMAMOTO A , et al . Hybrid rubisco with complete replacement of rice Rubisco small subunits by sorghum counterparts confers C 4 plant-like high catalytic activity [J ] . Molecular Plant , 2020 , 13 ( 11 ): 1570 - 1581 .

MAO Y W , CATHERALL E , DÍAZ-RAMOS A , et al . The small subunit of Rubisco and its potential as an engineering target [J ] . Journal of Experimental Botany , 2023 , 74 ( 2 ): 543 - 561 .

ADLER L , DÍAZ-RAMOS A , MAO Y W , et al . New horizons for building pyrenoid-based CO 2 -concentrating mechanisms in plants to improve yields [J ] . Plant Physiology , 2022 , 190 ( 3 ): 1609 - 1627 .

GONG H Y , LI Y , FANG G , et al . Transgenic rice expressing ictb and FBP/sbpase derived from cyanobacteria exhibits enhanced photosynthesis and mesophyll conductance to CO 2 [J ] . PLoS One , 2015 , 10 ( 10 ): e0140928 .

LONG B M , HEE W Y , SHARWOOD R E , et al . Carboxysome encapsulation of the CO 2 -fixing enzyme Rubisco in tobacco chloroplasts [J ] . Nature Communications , 2018 , 9 : 3570 .

CHEN T Y , HOJKA M , DAVEY P , et al . Engineering α-carboxysomes into plant chloroplasts to support autotrophic photosynthesis [J ] . Nature Communications , 2023 , 14 : 2118 .

FURBANK R T . Evolution of the C 4 photosynthetic mechanism: are there really three C 4 acid decarboxylation types? [J ] . Journal of Experimental Botany , 2011 , 62 ( 9 ): 3103 - 3108 .

FURBANK R , KELLY S , VON CAEMMERER S . Photosynthesis and food security: the evolving story of C 4 rice [J ] . Photosynthesis Research , 2023 , 158 ( 2 ): 121 - 130 .

ERMAKOVA M , DANILA F R , FURBANK R T , et al . On the road to C 4 rice: advances and perspectives [J ] . The Plant Journal , 2020 , 101 ( 4 ): 940 - 950 .

ERMAKOVA M , ARRIVAULT S , GIULIANI R , et al . Installation of C 4 photosynthetic pathway enzymes in rice using a single construct [J ] . Plant Biotechnology Journal , 2021 , 19 ( 3 ): 575 - 588 .

SMITH E N , VAN AALST M , TOSENS T , et al . Improving photosynthetic efficiency toward food security: strategies, advances, and perspectives [J ] . Molecular Plant , 2023 , 16 ( 10 ): 1547 - 1563 .

LIN X L , LONG Y M , YAO Z , et al . Synthetic photorespiratory bypass more stably increases potato yield per plant by improving photosynthesis [J ] . Plant Biotechnology Journal , 2025 , 23 ( 7 ): 2526 - 2536 .

MO B Q , CHEN X F , YANG J J , et al . Engineering of photorespiration-dependent glycine betaine biosynthesis improves photosynthetic carbon fixation and panicle architecture in rice [J ] . Journal of Integrative Plant Biology , 2025 , 67 ( 4 ): 979 - 992 .

WALTZ E . A new crop of microbe startups raises big bucks, takes on the establishment [J ] . Nature Biotechnology , 2017 , 35 ( 12 ): 1120 - 1122 .

ALLEN R S , TILBROOK K , WARDEN A C , et al . Expression of 16 nitrogenase proteins within the plant mitochondrial matrix [J ] . Frontiers in Plant Science , 2017 , 8 : 287 .

BIRCHLER J A , SWYERS N C . Engineered minichromosomes in plants [J ] . Experimental Cell Research , 2020 , 388 ( 2 ): 111852 .

OLDROYD G E , DIXON R . Biotechnological solutions to the nitrogen problem [J ] . Current Opinion in Biotechnology , 2014 , 26 : 19 - 24 .

BAGESHWAR U K , SRIVASTAVA M , PARDHA-SARADHI P , et al . An environmentally friendly engineered Azotobacter strain that replaces a substantial amount of urea fertilizer while sustaining the same wheat yield [J ] . Applied and Environmental Microbiology , 2017 , 83 ( 15 ): e00590-17 .

GEDDES B A , PARAMASIVAN P , JOFFRIN A , et al . Engineering transkingdom signalling in plants to control gene expression in rhizosphere bacteria [J ] . Nature Communications , 2019 , 10 : 3430 .

BLOCH S E , CLARK R , GOTTLIEB S S , et al . Biological nitrogen fixation in maize: optimizing nitrogenase expression in a root-associated diazotroph [J ] . Journal of Experimental Botany , 2020 , 71 ( 15 ): 4591 - 4603 .

CHRISTIAENS O , TARDAJOS M G , MARTINEZ REYNA Z L , et al . Increased RNAi efficacy in Spodoptera exigua via the formulation of dsRNA with guanylated polymers [J ] . Frontiers in Physiology , 2018 , 9 : 316 .

HUAN Y C , KONG Q , MOU H J , et al . Antimicrobial peptides: classification, design, application and research progress in multiple fields [J ] . Frontiers in Microbiology , 2020 , 11 : 582779 .

MARCOS J F , MUÑOZ A , PÉREZ-PAYÁ E , et al . Identification and rational design of novel antimicrobial peptides for plant protection [J ] . Annual Review of Phytopathology , 2008 , 46 : 273 - 301 .

MONTESINOS E . Functional peptides for plant disease control [J ] . Annual Review of Phytopathology , 2023 , 61 : 301 - 324 .

ZEITLER B , BERNHARD A , MEYER H , et al . Production of a de-novo designed antimicrobial peptide in Nicotiana benthamiana [J ] . Plant Molecular Biology , 2013 , 81 ( 3 ): 259 - 272 .

HOLÁSKOVÁ E , GALUSZKA P , MIČÚCHOVÁ A , et al . Molecular farming in barley: development of a novel production platform to produce human antimicrobial peptide LL-37 [J ] . Biotechnology Journal , 2018 , 13 ( 6 ): e1700628 .

MIRZAEE M , HOLÁSKOVÁ E , MIČÚCHOVÁ A , et al . Long-lasting stable expression of human LL-37 antimicrobial peptide in transgenic barley plants [J ] . Antibiotics , 2021 , 10 ( 8 ): 898 .

BUNDÓ M , SHI X Q , VERNET M , et al . Rice seeds as biofactories of rationally designed and cell-penetrating antifungal PAF peptides [J ] . Frontiers in Plant Science , 2019 , 10 : 731 .

KOCH A , KUMAR N , WEBER L , et al . Host-induced gene silencing of cytochrome P450 lanosterol C14α-demethylase-encoding genes confers strong resistance to Fusarium species [J ] . Proceedings of the National Academy of Sciences of the United States of America , 2013 , 110 ( 48 ): 19324 - 19329 .

SÚNICO V , HIGUERA J J , MOLINA-HIDALGO F J , et al . The intragenesis and synthetic biology approach towards accelerating genetic gains on strawberry: development of new tools to improve fruit quality and resistance to pathogens [J ] . Plants , 2022 , 11 ( 1 ): 57 .

MOORE B D , ANDREW R L , KÜLHEIM C , et al . Explaining intraspecific diversity in plant secondary metabolites in an ecological context [J ] . New Phytologist , 2014 , 201 ( 3 ): 733 - 750 .

AHUJA I , KISSEN R , BONES A M . Phytoalexins in defense against pathogens [J ] . Trends in Plant Science , 2012 , 17 ( 2 ): 73 - 90 .

HU H , LI J J , DELATTE T , et al . Modification of chrysanthemum odour and taste with chrysanthemol synthase induces strong dual resistance against cotton aphids [J ] . Plant Biotechnology Journal , 2018 , 16 ( 8 ): 1434 - 1445 .

MITTLER R , BLUMWALD E . Genetic engineering for modern agriculture: challenges and perspectives [J ] . Annual Review of Plant Biology , 2010 , 61 : 443 - 462 .

SHI H T , YE T T , CHAN Z L . Comparative proteomic responses of two bermudagrass ( Cynodon dactylon (L). Pers.) varieties contrasting in drought stress resistance [J ] . Plant Physiology and Biochemistry , 2014 , 82 : 218 - 228 .

HU H H , XIONG L Z . Genetic engineering and breeding of drought-resistant crops [J ] . Annual Review of Plant Biology , 2014 , 65 : 715 - 741 .

CHEN S J , XU K , KONG D Y , et al . Ubiquitin ligase Os RINGzf1 regulates drought resistance by controlling the turnover of Os PIP2;1 [J ] . Plant Biotechnology Journal , 2022 , 20 ( 9 ): 1743 - 1755 .

DING L , MILHIET T , PARENT B , et al . The plasma membrane aquaporin Zm PIP2;5 enhances the sensitivity of stomatal closure to water deficit [J ] . Plant , Cell & Environment, 2022 , 45 ( 4 ): 1146 - 1156 .

XU Y , HU W , LIU J H , et al . An aquaporin gene MaPIP2-7 is involved in tolerance to drought, cold and salt stresses in transgenic banana ( Musa acuminata L.) [J ] . Plant Physiology and Biochemistry , 2020 , 147 : 66 - 76 .

SAJA-GARBARZ D , LIBIK-KONIECZNY M , FELLNER M , et al . Silicon-induced alterations in the expression of aquaporins and antioxidant system activity in well-watered and drought-stressed oilseed rape [J ] . Plant Physiology and Biochemistry , 2022 , 174 : 73 - 86 .

HOSSEINIFARD M , STEFANIAK S , GHORBANI JAVID M , et al . Contribution of exogenous proline to abiotic stresses tolerance in plants: a review [J ] . International Journal of Molecular Sciences , 2022 , 23 ( 9 ): 5186 .

ARAÚJO W L , NUNES-NESI A , OSORIO S , et al . Antisense inhibition of the iron-sulphur subunit of succinate dehydrogenase enhances photosynthesis and growth in tomato via an organic acid-mediated effect on stomatal aperture [J ] . The Plant Cell , 2011 , 23 ( 2 ): 600 - 627 .

MOVAHEDI A , DZINYELA R , AGHAEI-DARGIRI S , et al . Advanced study of drought-responsive protein pathways in plants [J ] . Agronomy , 2023 , 13 ( 3 ): 849 .

EDWARDS R A , NG X Y , TUCKER M R , et al . Plant synthetic biology as a tool to help eliminate hidden hunger [J ] . Current Opinion in Biotechnology , 2024 , 88 : 103168 .

BEYENE G , SOLOMON F R , CHAUHAN R D , et al . Provitamin A biofortification of cassava enhances shelf life but reduces dry matter content of storage roots due to altered carbon partitioning into starch [J ] . Plant Biotechnology Journal , 2018 , 16 ( 6 ): 1186 - 1200 .

LOW J W , MWANGA R O M , ANDRADE M , et al . Tackling vitamin A deficiency with biofortified sweetpotato in sub-Saharan Africa [J ] . Global Food Security , 2017 , 14 : 23 - 30 .

LI K T , MOULIN M , MANGEL N , et al . Increased bioavailable vitamin B 6 in field-grown transgenic cassava for dietary sufficiency [J ] . Nature Biotechnology , 2015 , 33 ( 10 ): 1029 - 1032 .

DE LEPELEIRE J , STROBBE S , VERSTRAETE J , et al . Folate biofortification of potato by Tuber -specific expression of four folate biosynthesis genes [J ] . Molecular Plant , 2018 , 11 ( 1 ): 175 - 188 .

BULLEY S , LAING W . The regulation of ascorbate biosynthesis [J ] . Current Opinion in Plant Biology , 2016 , 33 : 15 - 22 .

WANG L Y , MENG X , YANG D Y , et al . Overexpression of tomato GDP-L-galactose phosphorylase gene in tobacco improves tolerance to chilling stress [J ] . Plant Cell Reports , 2014 , 33 ( 9 ): 1441 - 1451 .

ALI B , PANTHA S , ACHARYA R , et al . Enhanced ascorbate level improves multi-stress tolerance in a widely grown indica rice variety without compromising its agronomic characteristics [J ] . Journal of Plant Physiology , 2019 , 240 : 152998 .

MA L C , WANG Y R , LIU W X , et al . Overexpression of an alfalfa GDP-mannose 3,5-epimerase gene enhances acid, drought and salt tolerance in transgenic Arabidopsis by increasing ascorbate accumulation [J ] . Biotechnology Letters , 2014 , 36 ( 11 ): 2331 - 2341 .

LI X J , YE J , MUNIR S , et al . Biosynthetic gene pyramiding leads to ascorbate accumulation with enhanced oxidative stress tolerance in tomato [J ] . International Journal of Molecular Sciences , 2019 , 20 ( 7 ): 1558 .

ZHANG G Y , LIU R R , ZHANG C Q , et al . Manipulation of the rice L-galactose pathway: evaluation of the effects of transgene overexpression on ascorbate accumulation and abiotic stress tolerance [J ] . PLoS One , 2015 , 10 ( 5 ): e0125870 .

LI J , SCARANO A , GONZALEZ N M , et al . Biofortified tomatoes provide a new route to vitamin D sufficiency [J ] . Nature Plants , 2022 , 8 ( 6 ): 611 - 616 .

UPADHYAYA D C , BAGRI D S , UPADHYAYA C P , et al . Genetic engineering of potato ( Solanum tuberosum L.) for enhanced α-tocopherols and abiotic stress tolerance [J ] . Physiologia Plantarum , 2021 , 173 ( 1 ): 116 - 128 .

BOONYAVES K , WU T Y , GRUISSEM W , et al . Enhanced grain iron levels in rice expressing an IRON-REGULATED METAL TRANSPORTER, NICOTIANAMINE SYNTHASE , and FERRITIN gene cassette [J ] . Frontiers in Plant Science , 2017 , 8 : 130 .

NARAYANAN N , BEYENE G , CHAUHAN R D , et al . Biofortification of field-grown cassava by engineering expression of an iron transporter and ferritin [J ] . Nature Biotechnology , 2019 , 37 ( 2 ): 144 - 151 .

HARRINGTON S A , CONNORTON J M , NYANGOMA N I M , et al . A two-gene strategy increases iron and zinc concentrations in wheat flour, improving mineral bioaccessibility [J ] . Plant Physiology , 2023 , 191 ( 1 ): 528 - 541 .

NAGESH C R , PRASHAT G R , GOSWAMI S , et al . Sulfate transport and metabolism: strategies to improve the seed protein quality [J ] . Molecular Biology Reports , 2024 , 51 ( 1 ): 242 .

KIM W S , SUN-HYUNG J , OEHRLE N W , et al . Overexpression of ATP sulfurylase improves the sulfur amino acid content, enhances the accumulation of Bowman-Birk protease inhibitor and suppresses the accumulation of the β-subunit of β-conglycinin in soybean seeds [J ] . Scientific Reports , 2020 , 10 : 14989 .

HUANG Y C , WANG H H , ZHU Y D , et al . THP9 enhances seed protein content and nitrogen-use efficiency in maize [J ] . Nature , 2022 , 612 ( 7939 ): 292 - 300 .

LEE S , PARK J , LEE J , et al . OsASN1 overexpression in rice increases grain protein content and yield under nitrogen-limiting conditions [J ] . Plant & Cell Physiology , 2020 , 61 ( 7 ): 1309 - 1320 .

LIU X Q , LI S Z , YANG W Z , et al . Synthesis of seed-specific bidirectional promoters for metabolic engineering of anthocyanin-rich maize [J ] . Plant & Cell Physiology , 2018 , 59 ( 10 ): 1942 - 1955 .

GONZALI S , PERATA P . Anthocyanins from purple tomatoes as novel antioxidants to promote human health [J ] . Antioxidants , 2020 , 9 ( 10 ): 1017 .

BEYER P , AL-BABILI S , YE X D , et al . Golden Rice: introducing the beta-carotene biosynthesis pathway into rice endosperm by genetic engineering to defeat vitamin A deficiency [J ] . The Journal of Nutrition , 2002 , 132 ( 3 ): 506S - 510S .

HAN L H , SILVESTRE S , SAYANOVA O , et al . Using field evaluation and systematic iteration to rationalize the accumulation of omega-3 long-chain polyunsaturated fatty acids in transgenic Camelina sativa [J ] . Plant Biotechnology Journal , 2022 , 20 ( 9 ): 1833 - 1852 .

RYU M H , ZHANG J , TOTH T , et al . Control of nitrogen fixation in bacteria that associate with cereals [J ] . Nature Microbiology , 2020 , 5 ( 2 ): 314 - 330 .

SHULSE C N , CHOVATIA M , AGOSTO C , et al . Engineered root bacteria release plant-available phosphate from phytate [J ] . Applied and Environmental Microbiology , 2019 , 85 ( 18 ): e01210-19 .

SHAO J H , LI S Q , ZHANG N , et al . Analysis and cloning of the synthetic pathway of the phytohormone indole-3-acetic acid in the plant-beneficial Bacillus amyloliquefaciens SQR9 [J ] . Microbial Cell Factories , 2015 , 14 : 130 .

ZÚÑIGA A , DE LA FUENTE F , FEDERICI F , et al . An engineered device for indoleacetic acid production under quorum sensing signals enables Cupriavidus pinatubonensis JMP134 to stimulate plant growth [J ] . ACS Synthetic Biology , 2018 , 7 ( 6 ): 1519 - 1527 .

TRDÁ L , BAREŠOVÁ M , ŠAŠEK V , et al . Cytokinin metabolism of pathogenic fungus Leptosphaeria maculans involves isopentenyltransferase, adenosine kinase and cytokinin oxidase/dehydrogenase [J ] . Frontiers in Microbiology , 2017 , 8 : 1374 .

SALOMON M V , BOTTINI R , DE SOUZA FILHO G A , et al . Bacteria isolated from roots and rhizosphere of Vitis vinifera retard water losses, induce abscisic acid accumulation and synthesis of defense-related terpenes in in vitro cultured grapevine [J ] . Physiologia Plantarum , 2014 , 151 ( 4 ): 359 - 374 .

MULLINS A J , MURRAY J A H , BULL M J , et al . Genome mining identifies cepacin as a plant-protective metabolite of the biopesticidal bacterium Burkholderia ambifaria [J ] . Nature Microbiology , 2019 , 4 ( 6 ): 996 - 1005 .

LI Z L , HUANG P J , WANG M Y , et al . Stepwise increase of thaxtomins production in Streptomyces albidoflavus J1074 through combinatorial metabolic engineering [J ] . Metabolic Engineering , 2021 , 68 : 187 - 198 .

LIU Y P , ZHU A P , TAN H M , et al . Engineering banana endosphere microbiome to improve Fusarium wilt resistance in banana [J ] . Microbiome , 2019 , 7 ( 1 ): 74 .

SANATI NEZHAD A . Microfluidic platforms for plant cells studies [J ] . Lab on a Chip , 2014 , 14 ( 17 ): 3262 - 3274 .

MASSALHA H , KORENBLUM E , MALITSKY S , et al . Live imaging of root-bacteria interactions in a microfluidics setup [J ] . Proceedings of the National Academy of Sciences of the United States of America , 2017 , 114 ( 17 ): 4549 - 4554 .

KEHE J , KULESA A , ORTIZ A , et al . Massively parallel screening of synthetic microbial communities [J ] . Proceedings of the National Academy of Sciences of the United States of America , 2019 , 116 ( 26 ): 12804 - 12809 .

ZENGLER K , HOFMOCKEL K , BALIGA N S , et al . EcoFABs: advancing microbiome science through standardized fabricated ecosystems [J ] . Nature Methods , 2019 , 16 ( 7 ): 567 - 571 .

LIU P , PANDA K , EDWARDS S A , et al . Transposase-assisted target-site integration for efficient plant genome engineering [J ] . Nature , 2024 , 631 ( 8021 ): 593 - 600 .

邵洁 , 刘海利 , 王勇 . 植物合成生物学的现在与未来 [J ] . 合成生物学 , 2020 , 1 ( 4 ): 395 - 412 .

SHAO J , LIU H L , WANG Y . Present and future of plant synthetic biology [J ] . Synthetic Biology Journal , 2020 , 1 ( 4 ): 395 - 412 .

SANDHYA D , JOGAM P , ALLINI V R , et al . The present and potential future methods for delivering CRISPR/Cas9 components in plants [J ] . Journal of Genetic Engineering and Biotechnology , 2020 , 18 ( 1 ): 25 .

CHEN L , LIU G Q , ZHANG T . Integrating machine learning and genome editing for crop improvement [J ] . aBIOTECH , 2024 , 5 ( 2 ): 262 - 277 .

BAUER-PANSKUS A , MIYAZAKI J , KAWALL K , et al . Risk assessment of genetically engineered plants that can persist and propagate in the environment [J ] . Environmental Sciences Europe , 2020 , 32 ( 1 ): 32 .

LI J , ZHAO H M , ZHENG L X , et al . Advances in synthetic biology and biosafety governance [J ] . Frontiers in Bioengineering and Biotechnology , 2021 , 9 : 598087 .

王盼娣 , 熊小娟 , 付萍 , 等 . 《生物安全法》实施背景下对合成生物学的监管 [J ] . 华中农业大学学报 , 2021 , 40 ( 6 ): 231 - 245 .

WANG P D , XIONG X J , FU P , et al . Regulation of synthetic biology under background of implementing Biosafety Law [J ] . Journal of Huazhong Agricultural University , 2021 , 40 ( 6 ): 231 - 245 .

DE SOUZA A P , BURGESS S J , DORAN L , et al . Soybean photosynthesis and crop yield are improved by accelerating recovery from photoprotection [J ] . Science , 2022 , 377 ( 6608 ): 851 - 854 .

SPRONCKEN C C M , LIU P , MONNEY J , et al . Large-area, self-healing block copolymer membranes for energy conversion [J ] . Nature , 2024 , 630 ( 8018 ): 866 - 871 .

HE Y , NING T T , XIE T T , et al . Large-scale production of functional human serum albumin from transgenic rice seeds [J ] . Proceedings of the National Academy of Sciences of the United States of America , 2011 , 108 ( 47 ): 19078 - 19083 .