Recent advancements in non-biological component-augmented synthetic bio-hybrid systems

HUANG Yuqing; WU Han; LI Xiaobin; LIU Junyu; MA Shaohua; GE Jun; XING Xinhui; ZHANG Canyang

doi:10.12211/2096-8280.2025-048

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Recent advancements in non-biological component-augmented synthetic bio-hybrid systems

Invited Review | 更新时间：2025-09-05

- Recent advancements in non-biological component-augmented synthetic bio-hybrid systems
- Synthetic Biology Journal Vol. 6, Issue 4, Pages: 764-788(2025)
- 作者机构：
  
  1.清华大学深圳国际研究生院生物医药与健康工程研究院，广东深圳 518005
  2.工业生物催化教育部重点实验室（清华大学），北京 100084
  3.清华大学化学工程系，北京 100084
- 作者简介：
- 基金信息：
- DOI：10.12211/2096-8280.2025-048
  CLC： Q81
- Received：23 May 2025，
  
  Revised：2025-07-13，
  
  Published：31 August 2025
- 稿件说明：
移动端阅览
黄瑜晴，吴涵，李晓彬，刘君禹，马少华，戈钧，邢新会，张灿阳. 非生物元件增强的合成生物杂合体系研究进展［J］. 合成生物学， 2025， 6（4）： 764-788

HUANG Yuqing， WU Han， LI Xiaobin， LIU Junyu， MA Shaohua， GE Jun， XING Xinhui， ZHANG Canyang. Recent advancements in non-biological component-augmented synthetic bio-hybrid systems［J］. Synthetic Biology Journal， 2025， 6（4）： 764-788
黄瑜晴，吴涵，李晓彬，刘君禹，马少华，戈钧，邢新会，张灿阳. 非生物元件增强的合成生物杂合体系研究进展［J］. 合成生物学， 2025， 6（4）： 764-788 DOI： 10.12211/2096-8280.2025-048.

HUANG Yuqing， WU Han， LI Xiaobin， LIU Junyu， MA Shaohua， GE Jun， XING Xinhui， ZHANG Canyang. Recent advancements in non-biological component-augmented synthetic bio-hybrid systems［J］. Synthetic Biology Journal， 2025， 6（4）： 764-788 DOI： 10.12211/2096-8280.2025-048.

摘要

利用人工非生物元件对生命体进行设计与改造是合成生物学的一大机遇。开发非生物元件-生物元件合成生物杂合体系，可以突破天然生化反应的局限，实现生物元件与非生物元件的协同增效和功能超越，在生物制造和生物医药新应用领域前景广阔，已成为合成生物学研究中备受关注的前沿方向。然而，相比于类型繁多和功能丰富的非生物元件，现有杂合体系的功能还较为单一。针对这一问题，本文系统综述了近年来非生物元件-生物元件合成生物杂合体系的研究进展，依据体系类型进行分类总结，通过对典型研究的深入分析，在归纳其功能实现途径的基础上，进一步揭示了现有体系在功能扩展和机制解析方面的局限性，并展望了该领域在多平台联用、工程化设计和精准调控等方面的发展前景。

Abstract

The integration of non-biological components into living systems represents a pivotal advance in synthetic biology. This approach facilitates the creation of synthe

tic bio-hybrid systems effectively overcoming inherent limitations of natural biological systems. By leveraging the synergistic enhancement and superior functionalities derived from both biological and non-biological constituents

these hybrid systems exhibit immense potential across diverse applications

including bio-manufacturing

precise diagnostics

and biomedicine

establishing them as a cutting-edge frontier. However

despite the vast functional diversity of non-biological components

current bio-hybrid systems often present functional singularity

and their underlying synergistic mechanisms remain insufficiently elucidated. These limitations hinder their broader adoption and sophisticated applications. To address these challenges

this review systematically summarizes recent advancements in non-biological component-enhanced synthetic bio-hybrid systems. We categorize these systems based on the nature of the non-biological components (

e.g

nanomaterials

polymers

semiconductors) and their integration strategies with diverse biological entities (

e.g

enzymes

nucleic acids

cells). Through in-depth analysis of representative studies

we elucidate construction methodologies

functional realization pathways

and performance characteristics across various hybrid configurations. A central focus is to critically identify existing limitations

particularly concerning functional modularity

fine-tuned control

and the comprehensive elucidation of complex underlying mechanisms. We also explore strategies to overcome these challenges

emphasizing rational design and advanced characterization. Looking ahead

we present a forward-looking perspective on the future trajectory of this burgeoning field. Key areas for advancement include multi-platform integration

combining various non-biological components with multiple biological parts for highly sophisticated systems. Furthermore

we highlight the importance of advanced engineering design and high-throughput screening to accelerate

discovery and optimization. The refinement of precise spatiotemporal regulation is crucial for controlling complex assemblies. Moreover

the integration of artificial intelligence and machine learning for rational design promises to revolutionize development. This review aims to serve as a valuable resource

providing critical insights and inspiring further research into the design

construction

and application of non-biological component-enhanced synthetic bio-hybrid systems

therefore paving the way for groundbreaking innovations in healthcare and biotechnology.

关键词

Keywords

references

BYRNE G , DIMITROV D , MONOSTORI L , et al . Biologicalisation: biological transformation in manufacturing [J ] . CIRP Journal of Manufacturing Science and Technology , 2018 , 21 : 1 - 32 .

BERGS T , SCHWANEBERG U , BARTH S , et al . Application cases of biological transformation in manufacturing technology [J ] . CIRP Journal of Manufacturing Science and Technology , 2020 , 31 : 68 - 77 .

VIJAYARAM S , RAZAFINDRALAMBO H , SUN Y Z , et al . Applications of green synthesized metal nanoparticles: a review [J ] . Biological Trace Element Research , 2024 , 202 ( 1 ): 360 - 386 .

张晓龙 , 王晨芸 , 刘延峰 , 等 . 基于合成生物技术构建高效生物制造系统的研究进展 [J ] . 合成生物学 , 2021 , 2 ( 6 ): 863 - 875 .

ZHANG X L , WANG C Y , LIU Y F , et al . Research progress of constructing efficient biomanufacturing system based on synthetic biotechnology [J ] . Synthetic Biology Journal , 2021 , 2 ( 6 ): 863 - 875 .

LI X Y , CAO Y F , LUO K , et al . Highly active enzyme-metal nanohybrids synthesized in protein-polymer conjugates [J ] . Nature Catalysis , 2019 , 2 ( 8 ): 718 - 725 .

LU H F , OUYANG J P , LIU W Q , et al . Enzyme-polymer-conjugate-based pickering emulsions for cell-free expression and cascade biotransformation [J ] . Angewandte Chemie International Edition , 2023 , 62 ( 52 ): e202312906 .

WEBER E W , MAUS M V , MACKALL C L . The emerging landscape of immune cell therapies [J ] . Cell , 2020 , 181 ( 1 ): 46 - 62 .

UDDIN F , RUDIN C M , SEN T . CRISPR gene therapy: applications, limitations, and implications for the future [J ] . Frontiers in Oncology , 2020 , 10 : 1387 .

UDDIN M N , RONI M A . Challenges of storage and stability of mRNA-based COVID-19 vaccines [J ] . Vaccines , 2021 , 9 ( 9 ): 1033 .

YIN Y , TANG W , MA X Y , et al . Biomimetic neutrophil and macrophage dual membrane-coated nanoplatform with orchestrated tumor-microenvironment responsive capability promotes therapeutic efficacy against glioma [J ] . Chemical Engineering Journal , 2022 , 433 : 133848 .

XIE B B , ZHAO H C , DING Y F , et al . Supramolecularly engineered conjugate of bacteria and cell membrane-coated magnetic nanoparticles for enhanced ferroptosis and immunotherapy of tumors [J ] . Advanced Science , 2023 , 10 ( 34 ): 2304407 .

WANG G H , XIE L S , LI B , et al . A nanounit strategy reverses immune suppression of exosomal PD-L1 and is associated with enhanced ferroptosis [J ] . Nature Communications , 2021 , 12 : 5733 .

TANG T C , AN B L , HUANG Y Y , et al . Materials design by synthetic biology [J ] . Nature Reviews Materials , 2021 , 6 ( 4 ): 332 - 350 .

PATRA P , DAS M , KUNDU P , et al . Recent advances in systems and synthetic biology approaches for developing novel cell-factories in non-conventional yeasts [J ] . Biotechnology Advances , 2021 , 47 : 107695 .

BARWINSKA-SENDRA A , GARCIA Y M , SENDRA K M , et al . An evolutionary path to altered cofactor specificity in a metalloenzyme [J ] . Nature Communications , 2020 , 11 : 2738 .

CAO Y F , LI X Y , GE J . Enzyme catalyst engineering toward the integration of biocatalysis and chemocatalysis [J ] . Trends in Biotechnology , 2021 , 39 ( 11 ): 1173 - 1183 .

LI X Y , FAN Y K , LIAO Q S , et al . Efficient enzyme-metal hybrid catalysts constructed with polymer [J ] . ChemCatChem , 2023 , 15 ( 1 ): e202201319 .

XIONG J R , CAI X Y , GE J . Enzyme-metal nanocomposites for antibacterial applications [J ] . Particuology , 2022 , 64 : 134 - 139 .

LI X Y , FU C C , LUO L Q , et al . Design of enzyme-metal hybrid catalysts for organic synthesis [J ] . Cell Reports Physical Science , 2022 , 3 ( 3 ): 100742 .

LIU W Q , ZHANG L K , CHEN M Z , et al . Cell-free protein synthesis: recent advances in bacterial extract sources and expanded applications [J ] . Biochemical Engineering Journal , 2019 , 141 : 182 - 189 .

YANG J , SUN Y , SHI H , et al . Small ligand-involved pickering droplet interface controls reaction selectivity of metal catalysts [J ] . Journal of the American Chemical Society , 2025 , 147 ( 7 ): 5984 - 5995 .

QIAO J , SONG Y Y , CHEN C F , et al . In situ determination of sialic acid on cell surface with a pH-regulated polymer enzyme nanoreactor [J ] . Analytical Chemistry , 2021 , 93 ( 19 ): 7317 - 7322 .

HONG S Y , CHOI D W , KIM H N , et al . Protein-based nanoparticles as drug delivery systems [J ] . Pharmaceutics , 2020 , 12 ( 7 ): 604 .

ZHANG C Y , DONG X Y , GAO J , et al . Nanoparticle-induced neutrophil apoptosis increases survival in sepsis and alleviates neurological damage in stroke [J ] . Science Advances , 2019 , 5 ( 11 ): eaax7964 .

WENG Y L , CHEN R , HUI Y , et al . Boosting enzyme activity in enzyme metal-organic framework composites [J ] . Chem & Bio Engineering , 2024 , 1 ( 2 ): 99 - 112 .

VAIDYA L B , NADAR S S , RATHOD V K . Entrapment of surfactant modified lipase within zeolitic imidazolate framework (ZIF)-8 [J ] . International Journal of Biological Macromolecules , 2020 , 146 : 678 - 686 .

NADAR S S , RATHOD V K . Immobilization of proline activated lipase within metal organic framework (MOF) [J ] . International Journal of Biological Macromolecules , 2020 , 152 : 1108 - 1112 .

BELL D J , WIESE M , SCHÖNBERGER A A , et al . Catalytically active hollow fiber membranes with enzyme-embedded metal-organic framework coating [J ] . Angewandte Chemie International Edition , 2020 , 59 ( 37 ): 16047 - 16053 .

XU Y Z , LIU S Y , LIU J L , et al . In situ enzyme immobilization with oxygen-sensitive luminescent metal-organic frameworks to realize “all-in-one” multifunctions [J ] . Chemistry-A European Journal , 2019 , 25 ( 21 ): 5463 - 5471 .

WANG Y F , TIAN G F , HUANG J , et al . Mussel-inspired protein-based nanoparticles for curcumin encapsulation and promoting antitumor efficiency [J ] . International Journal of Biological Macromolecules , 2024 , 273 : 132965 .

ORTEGA-NIETO C , LOSADA-GARCIA N , PESSELA B C , et al . Design and synthesis of copper nanobiomaterials with antimicrobial properties [J ] . ACS Bio & Med Chem Au , 2023 , 3 ( 4 ): 349 - 358 .

TONG P F , ASIF M , AJMAL M , et al . A multicomponent polymer-metal-enzyme system as electrochemical biosensor for H 2 O 2 detection [J ] . Frontiers in Chemistry , 2022 , 10 : 874965 .

DUAN L , OUYANG K , XU X , et al . Nanoparticle delivery of CRISPR/Cas9 for genome editing [J ] . Frontiers in Genetics , 2021 , 12 : 673286 .

EREL-AKBABA G , CARVALHO L A , TIAN T , et al . Radiation-induced targeted nanoparticle-based gene delivery for brain tumor therapy [J ] . ACS Nano , 2019 , 13 ( 4 ): 4028 - 4040 .

MADKHALI O , MEKHAIL G , WETTIG S D . Modified gelatin nanoparticles for gene delivery [J ] . International Journal of Pharmaceutics , 2019 , 554 : 224 - 234 .

WU J Y , CHEN J , FENG Y J , et al . An immune cocktail therapy to realize multiple boosting of the cancer-immunity cycle by combination of drug/gene delivery nanoparticles [J ] . Science Advances , 2020 , 6 ( 40 ): eabc7828 .

YANG Q , ZHOU Y H , CHEN J , et al . Gene therapy for drug-resistant glioblastoma via lipid-polymer hybrid nanoparticles combined with focused ultrasound [J ] . International Journal of Nanomedicine , 2021 , 16 : 185 - 199 .

EYGERIS Y , GUPTA M , KIM J , et al . Chemistry of lipid nanoparticles for RNA delivery [J ] . Accounts of Chemical Research , 2022 , 55 ( 1 ): 2 - 12 .

EYGERIS Y , PATEL S , JOZIC A , et al . Deconvoluting lipid nanoparticle structure for messenger RNA delivery [J ] . Nano Letters , 2020 , 20 ( 6 ): 4543 - 4549 .

FRANCIA V , SCHIFFELERS R M , CULLIS P R , et al . The biomolecular corona of lipid nanoparticles for gene therapy [J ] . Bioconjugate Chemistry , 2020 , 31 ( 9 ): 2046 - 2059 .

KROHN-GRIMBERGHE M , MITCHELL M J , SCHLOSS M J , et al . Nanoparticle-encapsulated siRNAs for gene silencing in the haematopoietic stem-cell niche [J ] . Nature Biomedical Engineering , 2020 , 4 ( 11 ): 1076 - 1089 .

MIRZA Z , KARIM S . Nanoparticles-based drug delivery and gene therapy for breast cancer: recent advancements and future challenges [J ] . Seminars in Cancer Biology , 2021 , 69 : 226 - 237 .

CHENG Q , WEI T , FARBIAK L , et al . Selective organ targeting (SORT) nanoparticles for tissue-specific mRNA delivery and CRISPR-Cas gene editing [J ] . Nature Nanotechnology , 2020 , 15 ( 4 ): 313 - 320 .

WANG Z C , WANG R R , GENG Z X , et al . Enzyme hybrid nanoflowers and enzyme@metal-organic frameworks composites: fascinating hybrid nanobiocatalysts [J ] . Critical Reviews in Biotechnology , 2024 , 44 ( 4 ): 674 - 697 .

ZHUANG J , GONG H , ZHOU J R , et al . Targeted gene silencing in vivo by platelet membrane-coated metal-organic framework nanoparticles [J ] . Science Advances , 2020 , 6 ( 13 ): eaaz6108 .

POSTLER T S , GHOSH S . Understanding the holobiont: how microbial metabolites affect human health and shape the immune system [J ] . Cell Metabolism , 2017 , 26 ( 1 ): 110 - 130 .

SUN J B , LI Y , LIANG X J , et al . Bacterial magnetosome: a novel biogenetic magnetic targeted drug carrier with potential multifunctions [J ] . Journal of Nanomaterials , 2011 , 2011 ( 1 ): 469031 .

CAO Z P , LIU J Y . Bacteria and bacterial derivatives as drug carriers for cancer therapy [J ] . Journal of Controlled Release , 2020 , 326 : 396 - 407 .

SHIVALKAR S , CHOWDHARY P , AFSHAN T , et al . Nanoengineering of biohybrid micro/nanobots for programmed biomedical applications [J ] . Colloids and Surfaces B: Biointerfaces , 2023 , 222 : 113054 .

AKOLPOGLU M B , ALAPAN Y , DOGAN N O , et al . Magnetically steerable bacterial microrobots moving in 3D biological matrices for stimuli-responsive cargo delivery [J ] . Science Advances , 2022 , 8 ( 28 ): eabo6163 .

ALAPAN Y , YASA O , YIGIT B , et al . Microrobotics and microorganisms: biohybrid autonomous cellular robots [J ] . Annual Review of Control, Robotics, and Autonomous Systems , 2019 , 2 : 205 - 230 .

AKIN D , STURGIS J , RAGHEB K , et al . Bacteria-mediated delivery of nanoparticles and cargo into cells [J ] . Nature Nanotechnology , 2007 , 2 ( 7 ): 441 - 449 .

KAUR P , GHOSH S , BHOWMICK A , et al . Bacterioboat—a novel tool to increase the half-life period of the orally administered drug [J ] . Science Advances , 2022 , 8 ( 10 ): eabh1419 .

ZOU Z P , DU Y , FANG T T , et al . Biomarker-responsive engineered probiotic diagnoses, records, and ameliorates inflammatory bowel disease in mice [J ] . Cell Host & Microbe , 2023 , 31 ( 2 ): 199 - 212.e5 .

WANG X Y , CAO Z P , ZHANG M M , et al . Bioinspired oral delivery of gut microbiota by self-coating with biofilms [J ] . Science Advances , 2020 , 6 ( 26 ): eabb1952 .

CAO Z P , WANG X Y , PANG Y , et al . Biointerfacial self-assembly generates lipid membrane coated bacteria for enhanced oral delivery and treatment [J ] . Nature Communications , 2019 , 10 : 5783 .

LIN S S , MUKHERJEE S , LI J J , et al . Mucosal immunity-mediated modulation of the gut microbiome by oral delivery of probiotics into Peyer’s patches [J ] . Science Advances , 2021 , 7 ( 20 ): eabf0677 .

LIU J , WANG Y X , HEELAN W J , et al . Mucoadhesive probiotic backpacks with ROS nanoscavengers enhance the bacteriotherapy for inflammatory bowel diseases [J ] . Science Advances , 2022 , 8 ( 45 ): eabp8798 .

DE WOUTERS T , JANS C , NIEDERBERGER T , et al . Adhesion potential of intestinal microbes predicted by physico-chemical characterization methods [J ] . PLoS One , 2015 , 10 ( 8 ): e0136437 .

CHENG Z H , ZHENG Y Y , YANG W , et al . Pathogenic bacteria exploit transferrin receptor transcytosis to penetrate the blood-brain barrier [J ] . Proceedings of the National Academy of Sciences of the United States of America , 2023 , 120 ( 39 ): e2307899120 .

SUN R , LIU M Z , LU J P , et al . Bacteria loaded with glucose polymer and photosensitive ICG silicon-nanoparticles for glioblastoma photothermal immunotherapy [J ] . Nature Communications , 2022 , 13 : 5127 .

KARMAKAR R . State of the art of bacterial chemotaxis [J ] . Journal of Basic Microbiology , 2021 , 61 ( 5 ): 366 - 379 .

LI J H , DEKANOVSKY L , KHEZRI B , et al . Biohybrid micro- and nanorobots for intelligent drug delivery [J ] . Cyborg and Bionic Systems , 2022 , 2022 : 9824057 .

LI M , LI S Y , ZHOU H , et al . Chemotaxis-driven delivery of nano-pathogenoids for complete eradication of tumors post-phototherapy [J ] . Nature Communications , 2020 , 11 : 1126 .

LV X Y , WANG L C , MEI A Q , et al . Recent nanotechnologies to overcome the bacterial biofilm matrix barriers [J ] . Small , 2023 , 19 ( 6 ): 2206220 .

HAHN J S , DING S W , IM J W , et al . Bacterial therapies at the interface of synthetic biology and nanomedicine [J ] . Nature Reviews Bioengineering , 2024 , 2 ( 2 ): 120 - 135 .

WU D J , ZHAO Z J , LIU H , et al . Escherichia coli Nissle 1917-driven microrobots for effective tumor targeted drug delivery and tumor regression [J ] . Acta Biomaterialia , 2023 , 169 : 477 - 488 .

CHEN H T , LI Y Z , WANG Y J , et al . An engineered bacteria-hybrid microrobot with the magnetothermal bioswitch for remotely collective perception and imaging-guided cancer treatment [J ] . ACS Nano , 2022 , 16 ( 4 ): 6118 - 6133 .

TAN S W , WU T T , ZHANG D , et al . Cell or cell membrane-based drug delivery systems [J ] . Theranostics , 2015 , 5 ( 8 ): 863 - 881 .

XIA Z X , MU W W , YUAN S J , et al . Cell membrane biomimetic nano-delivery systems for cancer therapy [J ] . Pharmaceutics , 2023 , 15 ( 12 ): 2770 .

OROOJALIAN F , BEYGI M , BARADARAN B , et al . Immune cell membrane-coated biomimetic nanoparticles for targeted cancer therapy [J ] . Small , 2021 , 17 ( 12 ): 2006484 .

HUANG L L , NIE W D , ZHANG J F , et al . Cell-membrane-based biomimetic systems with bioorthogonal functionalities [J ] . Accounts of Chemical Research , 2020 , 53 ( 1 ): 276 - 287 .

CHEN H Y , DENG J , WANG Y , et al . Hybrid cell membrane-coated nanoparticles: a multifunctional biomimetic platform for cancer diagnosis and therapy [J ] . Acta Biomaterialia , 2020 , 112 : 1 - 13 .

ZHU L , ZHONG Y , WU S , et al . Cell membrane camouflaged biomimetic nanoparticles: focusing on tumor theranostics [J ] . Materials Today Bio , 2022 , 14 : 100228 .

NGUYEN P H D , JAYASINGHE M K , LE A H , et al . Advances in drug delivery systems based on red blood cells and their membrane-derived nanoparticles [J ] . ACS Nano , 2023 , 17 ( 6 ): 5187 - 5210 .

LIU B , WANG W M , FAN J L , et al . RBC membrane camouflaged Prussian blue nanoparticles for gamabutolin loading and combined chemo/photothermal therapy of breast cancer [J ] . Biomaterials , 2019 , 217 : 119301 .

XIE W , DENG W W , ZAN M H , et al . Cancer cell membrane camouflaged nanoparticles to realize starvation therapy together with checkpoint blockades for enhancing cancer therapy [J ] . ACS Nano , 2019 , 13 ( 3 ): 2849 - 2857 .

WU Y H , ZHU R T , ZHOU M Y , et al . Homologous cancer cell membrane-camouflaged nanoparticles target drug delivery and enhance the chemotherapy efficacy of hepatocellular carcinoma [J ] . Cancer Letters , 2023 , 558 : 216106 .

REN X L , YANG S P , YU N , et al . Cell membrane camouflaged bismuth nanoparticles for targeted photothermal therapy of homotypic tumors [J ] . Journal of Colloid and Interface Science , 2021 , 591 : 229 - 238 .

HU Q , JIA L L , ZHANG X L , et al . Accurate construction of cell membrane biomime tic graphene nanodecoys via purposeful surface engineering to improve screening efficiency of active components of traditional Chinese medicine [J ] . Acta Pharmaceutica Sinica B , 2022 , 12 ( 1 ): 394 - 405 .

NIE D , DAI Z , LI J L , et al . Cancer-cell-membrane-coated nanoparticles with a yolk-shell structure augment cancer chemotherapy [J ] . Nano Letters , 2020 , 20 ( 2 ): 936 - 946 .

WANG C X , WU B , WU Y T , et al . Camouflaging nanoparticles with brain metastatic tumor cell membranes: a new strategy to traverse blood-brain barrier for imaging and therapy of brain tumors [J ] . Advanced Functional Materials , 2020 , 30 ( 14 ): 1909369 .

VIJAYAN V , UTHAMAN S , PARK I K . Cell membrane-camouflaged nanoparticles: a promising biomimetic strategy for cancer theragnostics [J ] . Polymers , 2018 , 10 ( 9 ): 983 .

CAO Z C , LIU X , ZHANG W Q , et al . Biomimetic macrophage membrane-camouflaged nanoparticles induce ferroptosis by promoting mitochondrial damage in glioblastoma [J ] . ACS Nano , 2023 , 17 ( 23 ): 23746 - 23760 .

JIANG Q , LIU Y , GUO R R , et al . Erythrocyte-cancer hybrid membrane-camouflaged melanin nanoparticles for enhancing photothermal therapy efficacy in tumors [J ] . Biomaterials , 2019 , 192 : 292 - 308 .

GONG P , WANG Y F , ZHANG P F , et al . Immunocyte membrane-coated nanoparticles for cancer immunotherapy [J ] . Cancers , 2020 , 13 ( 1 ): 77 .

ZHAO Y , ZHANG H G , ZHANG Q X , et al . Research progress of neutrophil-mediated drug delivery strategies for inflammation-related disease [J ] . Pharmaceutics , 2023 , 15 ( 7 ): 1881 .

CHU D F , DONG X Y , SHI X T , et al . Neutrophil-based drug delivery systems [J ] . Advanced Materials , 2018 , 30 ( 22 ): 1706245 .

CHU D F , DONG X Y , ZHAO Q , et al . Photosensitization priming of tumor microenvironments improves delivery of nanotherapeutics via neutrophil infiltration [J ] . Advanced Materials , 2017 , 29 ( 27 ): 1701021 .

SU Y J , GAO J , DONG X Y , et al . Neutrophil-mediated delivery of nanocrystal drugs via photoinduced inflammation enhances cancer therapy [J ] . ACS Nano , 2023 , 17 ( 16 ): 15542 - 15555 .

KUMBHOJKAR N , PRAKASH S , FUKUTA T , et al . Neutrophils bearing adhesive polymer micropatches as a drug-free cancer immunotherapy [J ] . Nature Biomedical Engineering , 2024 , 8 ( 5 ): 579 - 592 .

VENTURINI C , PETROVIC FABIJAN A , FAJARDO LUBIAN A , et al . Biological foundations of successful bacteriophage therapy [J ] . EMBO Molecular Medicine , 2022 , 14 ( 7 ): e12435 .

LOH B , GONDIL V S , MANOHAR P , et al . Encapsulation and delivery of therapeutic phages [J ] . Applied and Environmental Microbiology , 2021 , 87 ( 5 ): e01979-20 .

SAMANANDA SINGH L . Nano-emulsion encapsulation for the efficient delivery of bacteriophage therapeutics [J ] . Biologicals , 2024 , 85 : 101725 .

OTERO J , GARCÍA-RODRÍGUEZ A , CANO-SARABIA M , et al . Biodistribution of liposome-encapsulated bacteriophages and their transcytosis during oral phage therapy [J ] . Frontiers in Microbiology , 2019 , 10 : 689 .

ŚLIWKA P , SKARADZIŃSKI G , DUSZA I , et al . Freeze-drying of encapsulated bacteriophage T4 to obtain shelf-stable dry preparations for oral application [J ] . Pharmaceutics , 2023 , 15 ( 12 ): 2792 .

HSU B B , PLANT I N , LYON L , et al . In situ reprogramming of gut bacteria by oral delivery [J ] . Nature Communications , 2020 , 11 : 5030 .

KARIMI M , MIRSHEKARI H , MOOSAVI BASRI S M , et al . Bacteriophages and phage-inspired nanocarriers for targeted delivery of therapeutic cargos [J ] . Advanced Drug Delivery Reviews , 2016 , 106 : 45 - 62 .

ATHANASOPOULOU K , DANEVA G N , ADAMOPOULOS P G , et al . Artificial intelligence: the milestone in modern biomedical research [J ] . BioMedInformatics , 2022 , 2 ( 4 ): 727 - 744 .

VASINA M , VELECKÝ J , PLANAS-IGLESIAS J , et al . Tools for computational design and high-throughput screening of therapeutic enzymes [J ] . Advanced Drug Delivery Reviews , 2022 , 183 : 114143 .

MILLER K , JOLDES G R , BOURANTAS G , et al . Biomechanical modeling and computer simulation of the brain during neurosurgery [J ] . International Journal for Numerical Methods in Biomedical Engineering , 2019 , 35 ( 10 ): e3250 .

FIGUEIRAS A , DOMINGUES C , JARAK I , et al . New advances in biomedical application of polymeric micelles [J ] . Pharmaceutics , 2022 , 14 ( 8 ): 1700 .

ROGACKA J , LABUS K . Metal-organic frameworks as highly effective platforms for enzyme immobilization-current developments and future perspectives [J/OL ] . Brazilian Journal of Chemical Engineering , 2024 [ 2025-07-01 ] . https://doi.org/10.1007/s43153-024-00513-4 https://doi.org/10.1007/s43153-024-00513-4 .

GUO F , XU Z H , ZHANG W D , et al . Facile synthesis of catalase@ZIF-8 composite by biomimetic mineralization for efficient biocatalysis [J ] . Bioprocess and Biosystems Engineering , 2021 , 44 ( 6 ): 1309 - 1319 .

WÖRHEIDE M A , KRUMSIEK J , KASTENMÜLLER G , et al . Multi-omics integration in biomedical research - a metabolomics-centric review [J ] . Analytica Chimica Acta , 2021 , 1141 : 144 - 162 .

REEL P S , REEL S , PEARSON E , et al . Using machine learning approaches for multi-omics data analysis: a review [J ] . Biotechnology Advances , 2021 , 49 : 107739 .

VANELLA R , KOVACEVIC G , DOFFINI V , et al . High-throughput screening, next generation sequencing and machine learning: advanced methods in enzyme engineering [J ] . Chemical Communications , 2022 , 58 ( 15 ): 2455 - 2467 .

KANEHISA M , FURUMICHI M , SATO Y , et al . KEGG: biological systems database as a model of the real world [J ] . Nucleic Acids Research , 2025 , 53 ( D1 ): D672 - D677 .

KORTEMME T . De novo protein design—from new structures to programmable functions [J ] . Cell , 2024 , 187 ( 3 ): 526 - 544 .

HRISTOZOV D , GOTTARDO S , SEMENZIN E , et al . Frameworks and tools for risk assessment of manufactured nanomaterials [J ] . Environment International , 2016 , 95 : 36 - 53 .

NASIR R . Developing CRISPR-based therapies for genetic diseases: Clinical trials and regulatory challenges [J ] . Indus Journal of Agriculture and Biology , 2025 , 4 ( 1 ): 13 - 26 .

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ZHANG Can Yang

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Key Laboratory of Industrial Biocatalysis， Ministry of Education； Tsinghua University

Ocean University of China

Shandong Energy Institute

Qingdao Institute of Bioenergy and Bioprocess Technology， Chinese Academy of Sciences， Key Laboratory of Photoelectric Conversion and Utilization of Solar Energy， Shandong C1 Refinery Engineering Research Center， Qingdao New Energy Shandong Laboratory

In vitro Synthetic Biology Center， Tianjin Institute of Industrial Biotechnology， Chinese Academy of Sciences

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