Nucleic acid biosensing

YAO Linxin; SONG Lu; LI Min; ZUO Xiaolei

doi:10.12211/2096-8280.2025-015

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Nucleic acid biosensing

Invited Review | 更新时间：2026-03-07

- Nucleic acid biosensing
- Synthetic Biology Journal Vol. 7, Issue 1, Pages: 66-92(2026)
- 作者机构：
  
  上海交通大学医学院附属仁济医院，分子医学研究院，上海 200127
- 作者简介：
- 基金信息：
- DOI：10.12211/2096-8280.2025-015
  CLC： Q71
- Received：13 March 2025，
  
  Revised：2025-04-15，
  
  Published：28 February 2026
- 稿件说明：
移动端阅览
姚林欣，宋璐，李敏，左小磊. 核酸生物传感［J］. 合成生物学， 2026， 7（1）： 66-92

YAO Linxin， SONG Lu， LI Min， ZUO Xiaolei. Nucleic acid biosensing［J］. Synthetic Biology Journal， 2026， 7（1）： 66-92
姚林欣，宋璐，李敏，左小磊. 核酸生物传感［J］. 合成生物学， 2026， 7（1）： 66-92 DOI： 10.12211/2096-8280.2025-015.

YAO Linxin， SONG Lu， LI Min， ZUO Xiaolei. Nucleic acid biosensing［J］. Synthetic Biology Journal， 2026， 7（1）： 66-92 DOI： 10.12211/2096-8280.2025-015.

摘要

脱氧核糖核酸（DNA）分子在生命体内发挥储存和传递遗传信息的生物学功能，在体外，它还可以作为一种可编程的分子自组装纳米材料。基于DNA双螺旋模型和碱基互补配对原则，研究人员开发了多种DNA自组装技术并以此构建了尺寸、形貌可控以及具有动态响应特性的二维和三维纳米结构。鉴于DNA纳米结构具有可控的尺寸、精确的寻址能力、可定制的生物功能、良好的生物相容性等特点，其已被广泛用于生物传感、生物成像、组织工程、药物递送等分子生物学研究领域。本文概述了利用DNA自组装技术构建的二维和三维DNA纳米结构，并分类讨论了DNA链置换驱动以及环境刺激驱动的DNA纳米结构动态变构，重点介绍了DNA纳米结构的生物传感应用，最后展望了基于DNA自组装技术构建的生物传感器的发展前景与面临挑战，包括提高DNA纳米结构的合成效率和稳定性、开发体内动态监测技术、建立多重检测与快速诊断方法以及探索与CRISPR技术联用的新发展方向。

Abstract

Deoxyribonucleic acid (DNA) molecules store and transmit genetic information in all living organisms and some viruses.

In vitro

it can be utilized as a programmable and versatile molecular self-assembling building block to construct functional materials. On the basis of the DNA double helix model and the specific rules of base complementary pairing

where adenine (A) with thymine (T) and cytosine (C) with guanine (G)

researchers have developed various DNA self-assembly techniques over the past few decades

including DNA tiling arrays such as DNA origami and laterally developed single strand tiling. These technologies have been employed to construct a variety of two- or three-dimensional nanoscale structures and devices with controllable sizes and morphologies

as well as dynamic response properties to external environmental stimulus. Researchers have continually demonstrated the exceptional construction capabilities of DNA molecules and have constructed a variety of DNA nanostructures: from simple four-arm nucleic acid junction to origami arrays up to 2～3 microns in size

from two-dimensional planar shapes to three-dimensional complex and twisted structures

and from simple nano-tweezers to command-executing DNA walkers. Due to the unparalleled programmability

precise addressability

editable biological functions

tissue permeability and inherent biocompatibility of DNA nanostructures

they have hold significant potentials in molecular biology research such as biosensing

bioimaging

tissue engineering

and drug delivery. In this review

first

we summarize the construction of two- and three-dimensional DNA nano

structures using various DNA self-assembly technologies. Then

the dynamic transformation of DNA nanostructures driven by two distinct types of driving forces have been categorized and discussed. Finally

the prospects of biosensors based on DNA self-assembly technologies

as well as challenges in this field including enhancing the efficiency and stability of synthesized structures

advancing dynamic monitoring technology in vivo

establishing multiplex and rapid detection methods

and exploring new directions for integration with CRISPR technology

have been explored.

关键词

Keywords

references

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