Application of plasma microbial breeding technology in biofabrication

ZHONG Naicai; CHEN Yuan; PAN Wenfeng; SU Xiaofeng; LIAO Jingwen; ZHAI Yinglei; ZHONG Jinyi

doi:10.12211/2096-8280.2025-005

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Application of plasma microbial breeding technology in biofabrication

Invited Review | 更新时间：2025-09-05

- Application of plasma microbial breeding technology in biofabrication
- Synthetic Biology Journal Vol. 6, Issue 4, Pages: 789-805(2025)
- 作者机构：
  
  1.广州先进技术研究所，广东广州 511458
  2.沈阳药科大学，辽宁沈阳 117004
  3.国家生物制造产业创新中心，广东深圳 518107
- 作者简介：
- 基金信息：
- DOI：10.12211/2096-8280.2025-005
  CLC： Q819
- Received：20 January 2025，
  
  Revised：2025-03-05，
  
  Published：31 August 2025
- 稿件说明：
移动端阅览
钟奶才，陈缘，潘文锋，苏小凤，廖景文，翟英雷，钟近艺. 等离子体微生物育种技术在生物制造中的应用进展［J］. 合成生物学， 2025， 6（4）： 789-805

ZHONG Naicai， CHEN Yuan， PAN Wenfeng， SU Xiaofeng， LIAO Jingwen， ZHAI Yinglei， ZHONG Jinyi. Application of plasma microbial breeding technology in biofabrication［J］. Synthetic Biology Journal， 2025， 6（4）： 789-805
钟奶才，陈缘，潘文锋，苏小凤，廖景文，翟英雷，钟近艺. 等离子体微生物育种技术在生物制造中的应用进展［J］. 合成生物学， 2025， 6（4）： 789-805 DOI： 10.12211/2096-8280.2025-005.

ZHONG Naicai， CHEN Yuan， PAN Wenfeng， SU Xiaofeng， LIAO Jingwen， ZHAI Yinglei， ZHONG Jinyi. Application of plasma microbial breeding technology in biofabrication［J］. Synthetic Biology Journal， 2025， 6（4）： 789-805 DOI： 10.12211/2096-8280.2025-005.

摘要

可持续绿色生物制造是各国关注的战略重点，但微生物性能仍是制约生物制造产业化的瓶颈之一。传统育种方法常面临育种周期长、效率低、成本高等问题，难以满足工业化生产对高效、稳定生产菌株的需求。低温等离子体技术作为一种高效、绿色环保的微生物育种方法，可通过刺激突变位点、提升突变效率、扩展突变范围，有效提高目标菌株性能和产品产量，为工业微生物改良提供重要助力。本文综述了等离子体诱变技术的理论基础、三种等离子体源（ARTP、DBD、CD）的技术特点、等离子体诱变作用机制及其与高通量筛选、传统诱变、理性育种等技术的联用进展，归纳出生物酶、有机酸、生物能源、生物材料等生物制造领域的典型等离子体育种案例，为相关领域的研究和产业化提供参考。未来需要开发基于空气源的新型等离子体发生器，在小型化的基础上实现低成本、低能耗、低温升；与高通量筛选和AI等技术相融合进行菌株精准诱变和高效育种，实现技术瓶颈突破，最终推动生物制造产业升级。

Abstract

Sustainable and green biomanufacturing has emerged as a strategic priority for nations globally; however

microbial performance remains one of the key constraints hindering the industrialization of biomanufacturing. Traditional breeding methods often face challenges such as long breeding cycles

low efficiency

and high costs

making it difficult to meet the demands for highly efficient and stable microbial strains in industrial-scale production. Low-temperature plasma technology

as an efficient and environmentally friendly method for microbial breeding

can stimulate mutation hotspots

enhance mutation efficiency

and expand mutation ranges

effectively improving the performance of target strains and product yields. By combining plasma mutagenesis’s advanced capabilities with other techniques

significant improvements in the performance and productivity of microbial strains can be achieved

thus driving the commercialization of sustainable bioprocesses. This review outlines the theoretical basis of plasma mutagenesis technology

the technical characteristics of three plasma sources (ARTP

DBD

and CD)

the mechanisms of plasma mutagenesis

and the progress of combining this technology with high-throughput screening

classic mutagenesis

and rational breeding methods. Furthermore

it summarizes typical plasma breeding cases in biomanufacturing fields such as bio-enzyme

organic acids

bioenergy

and biomaterials. These insights offer important references for research and industrialization in related fields. By combining plasma mutagenesis’s advanced capabilities with other techniques

significant improvements in the performance and productivity of microbial strains can be achieved

thus driving the commercialization of sustainable bioprocesses. The cases discussed in this review provide a practical understanding of how plasma mutagenesis can be applied to optimize microbial strains for industrial-scale production of valuable bioproducts

providing a reference for research and industrialization in related fields. In the future

it is essential to develop novel plasma generators based on air source

which

while being miniaturized

achieve low cost

low energy consumption

and minimal temperature rise. Integrating them with high-throughput screening and AI technologies will enable precise microbial mutagenesis and efficient strain breeding

thereby overcoming technical bottlenecks and ultimately advancing the biomanufacturing industry.

关键词

Keywords

references

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