Bioconversion of one carbon feedstocks for producing organic acids

YU Wei; GAO Jiaoqi; ZHOU Yongjin

doi:10.12211/2096-8280.2024-023

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Bioconversion of one carbon feedstocks for producing organic acids

Invited Review | 更新时间：2025-03-19

- Bioconversion of one carbon feedstocks for producing organic acids
  封面论文
- Synthetic Biology Journal Vol. 5, Issue 5, Pages: 1169-1188(2024)
- 作者机构：
  
  1.中国科学院大连化学物理研究所，生物技术研究部，辽宁大连 116023
  2.大连市能源生物技术重点实验室，辽宁大连 116023
- 作者简介：
- 基金信息：
- DOI：10.12211/2096-8280.2024-023
  CLC： Q81
- Received：19 March 2024，
  
  Revised：2024-06-04，
  
  Published：31 October 2024
- 稿件说明：
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禹伟，高教琪，周雍进. 一碳生物转化合成有机酸的研究进展［J］. 合成生物学， 2024， 5（5）： 1169-1188

YU Wei， GAO Jiaoqi， ZHOU Yongjin. Bioconversion of one carbon feedstocks for producing organic acids［J］. Synthetic Biology Journal， 2024， 5（5）： 1169-1188
禹伟，高教琪，周雍进. 一碳生物转化合成有机酸的研究进展［J］. 合成生物学， 2024， 5（5）： 1169-1188 DOI： 10.12211/2096-8280.2024-023.

YU Wei， GAO Jiaoqi， ZHOU Yongjin. Bioconversion of one carbon feedstocks for producing organic acids［J］. Synthetic Biology Journal， 2024， 5（5）： 1169-1188 DOI： 10.12211/2096-8280.2024-023.

摘要

有机酸在食品、医药、化工、农业等领域有着广泛的应用。目前有机酸的生产主要以微生物发酵法为主，采用糖类为原料，然而长此以往可能面临“与人争粮”的困境。CO、CO

、甲烷、甲醇和甲酸等含有一个碳原子的物质被称为一碳（one carbon，C

）资源，其来源广泛且价格低廉，有望成为生物制造的替代原料，且C

原料生物转化有助于缓解温室效应、助力“碳中和”目标。本文总结了近年来CO

、甲烷和甲醇生物合成3种重要有机酸（3-羟基丙酸、乳酸、琥珀酸）的研究进展，主要论述了C

生物利用途径、有机酸的生物合成途径以及代谢工程策略，也讨论了C

合成有机酸的挑战及应对措施，并展望了有机酸产业化新路线，尤其是化学催化与生物转化耦合以CO

为原料合成有机酸。本综述对于C

生物炼制以及有机酸产业升级具有一定的参考意义。

Abstract

Organic acids

as important platform chemicals

have been widely used in food

pharmaceutical

chemical industries and agriculture. Currently

microbial production of organic acids relies primarily on sugars as feedstocks

which may suffer from the competition with food and arable lands. One carbon (C

) molecules such as CO

methane

methanol and formic acid are widespread and inexpensive

which are considered as ideal feedstocks for future bio-manufacturing. Bioconversion of C

feedstocks toward the production of organic acids helps mitigate greenhouse effect and realize carbon neutrality. Therefore C

sources have been regarded as raw materials of third generation biorefinery

and natural C

utilizing microbes attracted increasing attention. Although some microorganisms have native biosynthetic pathway of organic acids

the production efficiency is usually lower than expected. This review summarizes the recent progress on the biosynthesis of organic acids (3-hydroxypropionic acid

lactic acid and succinic acid) from C

feedstocks using synthetic biology methods. First

the native C

utilizing pathways are summarized

including CO

metha

methanol and formic acid. Then the metabolic engineering strategies to improve organic acids production were systematically reviewed

including the optimization of rate-limiting enzymes expression

enhancement of the supply of precursor and cofactor

cofactor engineering

and inhibition of the product degradation. In addition

the challenges

solutions

and prospects of C

bioconversion to organic acids are also discussed

and coupling chemical catalysis and biological transformation may provide a promising industrial route for organic acids production. In particular

methanol is an ideal C

feedstock with many advantages like convenient storage and transportation

high liquid-to-liquid mass transfer efficiency

and it can also be massively produced from CO

by “liquid sunshine” technology. Therefore constructing high efficient methanol cell factory may enable organic acids production from CO

a carbon neutral production manner. This review may provide a guidance for C

biorefinery and industrial bioproduction of organic acids.

关键词

Keywords

references

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