Advance in the immobilization of hydrogenases

LEI Hangbin; HE Ning; LI Feixuan; DONG Lingling; WANG Shizhen

doi:10.12211/2096-8280.2024-022

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Advance in the immobilization of hydrogenases

Invited Review | 更新时间：2025-04-11

- Advance in the immobilization of hydrogenases
- Synthetic Biology Journal Vol. 5, Issue 6, Pages: 1485-1497(2024)
- 作者机构：
  
  1.厦门大学化学化工学院化学工程与生物工程系，福建厦门 361005
  2.厦门大学厦门市合成生物学重点实验室，福建厦门 361005
- 作者简介：
- 基金信息：
- DOI：10.12211/2096-8280.2024-022
  CLC： Q814.2
- Received：11 March 2024，
  
  Revised：2024-05-17，
  
  Published：31 December 2024
- 稿件说明：
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雷航彬，何宁，李斐煊，董玲玲，王世珍. 氢化酶固定化研究进展［J］. 合成生物学， 2024， 5（6）： 1485-1497

LEI Hangbin， HE Ning， LI Feixuan， DONG Lingling， WANG Shizhen. Advance in the immobilization of hydrogenases［J］. Synthetic Biology Journal， 2024， 5（6）： 1485-1497
雷航彬，何宁，李斐煊，董玲玲，王世珍. 氢化酶固定化研究进展［J］. 合成生物学， 2024， 5（6）： 1485-1497 DOI： 10.12211/2096-8280.2024-022.

LEI Hangbin， HE Ning， LI Feixuan， DONG Lingling， WANG Shizhen. Advance in the immobilization of hydrogenases［J］. Synthetic Biology Journal， 2024， 5（6）： 1485-1497 DOI： 10.12211/2096-8280.2024-022.

摘要

氢化酶催化氢气向质子和电子的可逆转化，具有广阔的工业应用前景。但游离的氢化酶存在着对氧气敏感、传递电子速率慢等缺点。本文综述了碳材料、金属及半导体、高分子和金属-有机框架材料（MOF）固定化氢化酶。碳材料具有价格低廉、比表面积大等优势。金属及半导体有着良好的导电性能和优异的催化性能。高分子材料具有良好的生物相容性和机械性能，可以提高氢化酶的稳定性和对氧气的耐受性。MOF比表面积大，可设计调控，为理化性质不同的氢化酶提供了广泛的载体选择。复合材料固定化氢化酶可以结合不同材料的优势，拓宽固定化氢化酶的应用场景。固定化氢化酶可用于氢气的高效生产与应用以及生物不对称加氢制备手性化合物，为转变能源结构、实现绿色转型、解决环境问题提供了可选方案。

Abstract

Hydrogenases catalyze the reversible conversion of hydrogen gas into protons and electrons which is promising for industrial application. However

free hydrogenases face challenges such as oxygen sensitivity and low electron transfer rates. This review summarized the immobilization of hydrogenases by carbon materials

metals

semiconductors

polymers and metal-organic-frameworks (MOFs). Carbon materials provide the advantages of low cost and large specific surface areas

while they tend to agglomerate. Hydrogenases are immobilizated on carbon materials through adsorption

usually involving electrostatic interactions and hydrophobic interactions

and are used in bioelectrocatalysis

biofuel cells and bioreactors. Metals and semiconductors

known for high conductivity and excellent reactive activity

are expensive and less stable. Through adsorption involving electrostatic interaction and hydrophobic interaction

immobilization of hydrogenases on metals and semiconductors are normally applied in bioelectrocatalysis

biofuel cells and photoelectrocatalysis. Polymers have good biocompatibility and mechanical strength but low conductivity. Immobilization of hydrogenases on polymers can improve the stability and oxygen tolerance of hydrogenases. Immobilization on polymers is realized through adsorption and entrapment

involving hydrogen bonds

hydrophobic interactions and π-π interactions

and is often used in bioelectrocatalysis and photoelectrocatalysis. MOFs are designable and have high specific surface areas

which provide wide choices for hydrogenases immobilization. However

MOFs tend to collapse in harsh conditions. Immobilization on MOFs through adsorption and entrapment involves coordinate bonds

hydrophobic interaction

and π-π interaction. Furthermore

the prospect of immobilization of hydrogenases by novel hybrid materials was proposed which can expand the applications of immobilized hydrogenases. Immobilization of hydrogenases facilitates the stability of hydrogenases

which can be applied in efficient production and application of hydrogen

as well as biological asymmetric hydrogenation for chiral medicine preparation. Immobilization of hydrogenases provide alternative options for transforming energy structures

realizing green manufacturing and solving environmental problems.

关键词

Keywords

references

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