Data writing in DNA storage systems

ZHANG Xuanliang; LI Qingting; WANG Fei

doi:10.12211/2096-8280.2024-003

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Data writing in DNA storage systems

Invited Review | 更新时间：2025-03-19

- Data writing in DNA storage systems
- Synthetic Biology Journal Vol. 5, Issue 5, Pages: 1125-1141(2024)
- 作者机构：
  
  上海交通大学化学化工学院，变革性分子前沿科学中心，致远学院，上海 200240
- 作者简介：
- 基金信息：
- DOI：10.12211/2096-8280.2024-003
  CLC： Q816
- Received：02 January 2024，
  
  Revised：2024-05-20，
  
  Published：31 October 2024
- 稿件说明：
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张宣梁，李青婷，王飞. DNA存储系统中的数据写入［J］. 合成生物学， 2024， 5（5）： 1125-1141

ZHANG Xuanliang， LI Qingting， WANG Fei. Data writing in DNA storage systems［J］. Synthetic Biology Journal， 2024， 5（5）： 1125-1141
张宣梁，李青婷，王飞. DNA存储系统中的数据写入［J］. 合成生物学， 2024， 5（5）： 1125-1141 DOI： 10.12211/2096-8280.2024-003.

ZHANG Xuanliang， LI Qingting， WANG Fei. Data writing in DNA storage systems［J］. Synthetic Biology Journal， 2024， 5（5）： 1125-1141 DOI： 10.12211/2096-8280.2024-003.

摘要

世界的数字化给人们的生活带来了极大的变化，但与此同时，史无前例的数据激增使得信息存储面临的挑战日益严峻。随着全球数据总量的指数级增长，传统存储介质将无法满足数字化带来的存储需求。使用DNA分子作为基本载体的信息存储展现出高存储密度、低维护成本和易于化学修饰等独特优势。DNA存储主要包括编码、写入、保存、检索、读取和解码六个主要步骤，其中数据的写入是实现DNA存储功能的基础。本文首先介绍DNA存储系统中体外写入数据的策略方法，主要分为将数据写入DNA序列和写入DNA结构两个部分，接着概述体内写入数据技术的发展，最后将讨论DNA存储系统中数据写入面临的写入成本高、写入速度慢等挑战，并对大规模合成高纯度DNA、改进生物酶等具有前景的应用技术进行展望。

Abstract

Advances in science and technology are creating huge benefits and value for society. The digitalization of the world has brought great changes to human being’s daily life. Meanwhile

the increasing degree of digitalization has led to an unprecedented explosion of data

resulting in increasingly severe information storage challenges. According to the current developing trend

the global data volume is expected to reach 175 zettabytes by 2025. With the rapid growth of global data volume and the exponential growth of total data

the existing storage methods will no longer be able to meet the storage needs brought by the digitalization of the world and then there is an urgent need to develop information storage methods with better storage performance

higher storage efficiency and more durable storage media. Nature has offered a powerful solution by using DNA molecules as carriers of information

where genetic information has been transferred stably more than a million years. DNA storage has many advantages over traditional storage media

including high storage density

potentially low maintenance costs

and ease of synthesis and chemical modification

which make it an ideal alternative for information storage. The current process of storing data in DNA includes six main steps: encoding

writing

preservation

retrieval

reading

and decoding. Among them

the writing of data is the basic for realizing the storage of data in DNA

concluding writing data in DNA sequence and in DNA structure. In this review

we first introduce strategies for

in vivo

data writing in DNA storage systems

which primarily involve writing data into DNA sequences and DNA structures. This is followed by an overview of the development of

in vivo

writing techniques in DNA storage systems. Finally

we discuss the challenges faced by DNA storage systems in terms of high writing costs and slow writing speeds

and prospects for l

arge-scale synthesis of high-purity DNA and improved biocatalysts.

关键词

Keywords

references

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