

浏览全部资源
扫码关注微信
军事科学院军事医学研究院,病原微生物生物安全全国重点实验室,北京 100071
Received:09 August 2023,
Revised:2023-11-02,
Published:30 April 2024
移动端阅览
叶精勤, 黄文华, 潘超, 朱力, 王恒樑. 合成生物学在多糖结合疫苗研发中的应用[J]. 合成生物学, 2024, 5(2): 338-352
YE Jingqin, HUANG Wenhua, PAN Chao, ZHU Li, WANG Hengliang. Applications of synthetic biology in developing polysaccharide conjugate vaccines[J]. Synthetic Biology Journal, 2024, 5(2): 338-352
叶精勤, 黄文华, 潘超, 朱力, 王恒樑. 合成生物学在多糖结合疫苗研发中的应用[J]. 合成生物学, 2024, 5(2): 338-352 DOI: 10.12211/2096-8280.2023-054.
YE Jingqin, HUANG Wenhua, PAN Chao, ZHU Li, WANG Hengliang. Applications of synthetic biology in developing polysaccharide conjugate vaccines[J]. Synthetic Biology Journal, 2024, 5(2): 338-352 DOI: 10.12211/2096-8280.2023-054.
由于合成生物学的快速发展,目前已经实现了人工设计DNA和蛋白质的合成,对具有重要生物学功能、结构也更为复杂的糖类物质进行精准设计合成,也将是合成生物学未来发展的重要方向。近年来,一种基于细菌寡糖转移酶体系的蛋白多糖偶联技术发展迅速,已被广泛应用于病原细菌多糖结合疫苗的生物合成制备。本文综述了该技术体系中的寡糖转移酶元件、载体蛋白元件、异源多糖抗原合成线路以及工程菌株改造等核心关键模块的最新研究进展。使用生物活体系统,发酵生产多糖结合疫苗,具有产物均一性好、步骤简便、绿色环保等优势,是一种亟待发展的新兴技术,同时也存在一些技术细节需要完善。未来,寡糖转移酶的定向进化、纳米颗粒型蛋白载体的应用、多糖合成基因的组合重排、工程菌株的代谢途径优化,将有望进一步促进多糖结合疫苗的生物合成研究。
The precise design and synthesis of carbohydrates with important biological functions and more complex structures is a frontier in synthetic biology. Recently
a novel strategy named Protein Glycan Coupling Technology (PGCT) based on bacterial oligosaccharyltransferases has been developed and widely used in the biosynthesis of bacterial glycoconjugate vaccines
which are one of achievements in modern medicine due to their effectiveness in fighting against infectious diseases. Herein
progress in developing key components for manufacturing glycoconjugate vaccines
such as oligosaccharyltransferases (PglL
PglS
PglB
and TfmP)
carrier proteins (CRM197
diphtheria toxoid
recombinant
Pseudomonas aeruginosa
exotoxin A
and nanoparticles)
polysaccharide biosynthesis gene circuits
and glyco-engineered strains is reviewed. Meanwhile
producing glycoconjugate vaccines through fermentation presents advantages in good product quality control for safety and efficacy
low production cost
and environmental-friendly manufacturing. PGCT has potentials to overcome some limitations of chemical conjugation production processes
such as complex purification and high cost
for competitiveness with existing chemical conjugates. As an emerging technology
more technological i
nnovations are needed for PGCT. In the future
the directed evolution of oligosaccharyltransferases
the application of protein nanoparticle carriers
the combination rearrangement of glycosyltransferases
and the optimization of engineered bacterial strains with better metabolic pathways are expected to further promote the biosynthesis of conjugate vaccines. The next few years will be an important and exciting time for PGCT
as recent technological advances are being applied to the development of novel glycoconjugates
and ongoing large-scale clinic trials on the efficacy of glycoconjugate vaccines will also demonstrate the feasibility of this technology
making the future of PGCT vaccinology promising.
2
ANDRIANANTOANDRO E , BASU S , KARIG D K , et al . Synthetic biology: new engineering rules for an emerging discipline [J ] . Molecular Systems Biology , 2006 , 2 : 2006 .0028.
BRENNER K , YOU L C , ARNOLD F H . Engineering microbial consortia: a new frontier in synthetic biology [J ] . Trends in Biotechnology , 2008 , 26 ( 9 ): 483 - 489 .
JAROENTOMEECHAI T , TAW M N , LI M J , et al . Cell-free synthetic glycobiology: designing and engineering glycomolecules outside of living cells [J ] . Frontiers in Chemistry , 2020 , 8 : 645 .
KHOURY G A , BALIBAN R C , FLOUDAS C A . Proteome-wide post-translational modification statistics: frequency analysis and curation of the swiss-prot database [J ] . Scientific Reports , 2011 , 1 : 90 .
HELENIUS A , AEBI A M . Intracellular functions of N -linked glycans [J ] . Science , 2001 , 291 ( 5512 ): 2364 - 2369 .
RUDD P M , WORMALD M R , STANFIELD R L , et al . Roles for glycosylation of cell surface receptors involved in cellular immune recognition [J ] . Journal of Molecular Biology , 1999 , 293 ( 2 ): 351 - 366 .
TYTGAT H L P , LEBEER S . The sweet tooth of bacteria: common themes in bacterial glycoconjugates [J ] . Microbiology and Molecular Biology Reviews: MMBR , 2014 , 78 ( 3 ): 372 - 417 .
STRENG-OUWEHAND I , HO N I , LITJENS M , et al . Glycan modification of antigen alters its intracellular routing in dendritic cells, promoting priming of T cells [J ] . eLife , 2016 , 5 : e11765 .
PHANSE Y , CARRILLO-CONDE B R , RAMER-TAIT A E , et al . A systems approach to designing next generation vaccines: combining α-galactose modified antigens with nanoparticle platforms [J ] . Scientific Reports , 2014 , 4 : 3775 .
ELLIOTT S , LORENZINI T , ASHER S , et al . Enhancement of therapeutic protein in vivo activities through glycoengineering [J ] . Nature Biotechnology , 2003 , 21 ( 4 ): 414 - 421 .
OHTSUBO K , MARTH J D . Glycosylation in cellular mechanisms of health and disease [J ] . Cell , 2006 , 126 ( 5 ): 855 - 867 .
ZHANG Q , JOHNSTON E V , SHIEH J H , et al . Synthesis of granulocyte-macrophage colony-stimulating factor as homogeneous glycoforms and early comparisons with yeast cell-derived material [J ] . Proceedings of the National Academy of Sciences of the United States of America , 2014 , 111 ( 8 ): 2885 - 2890 .
CHUNG C H , MIRAKHUR B , CHAN E , et al . Cetuximab-induced anaphylaxis and IgE specific for galactose-α-1,3-galactose [J ] . New England Journal of Medicine , 2008 , 358 ( 11 ): 1109 - 1117 .
LI H J , SETHURAMAN N , STADHEIM T A , et al . Optimization of humanized IgGs in glycoengineered Pichia pastoris [J ] . Nature Biotechnology , 2006 , 24 ( 2 ): 210 - 215 .
TIAN W H , YE Z L , WANG S J , et al . The glycosylation design space for recombinant lysosomal replacement enzymes produced in CHO cells [J ] . Nature Communications , 2019 , 10 : 1785 .
ABREU A G , BARBOSA A S . How Escherichia coli circumvent complement-mediated killing [J ] . Frontiers in Immunology , 2017 , 8 : 452 .
BUNDLE D . Antibacterials: a sweet vaccine [J ] . Nature Chemistry , 2016 , 8 ( 3 ): 201 - 202 .
SZYMANSKI C M , YAO R J , EWING C P , et al . Evidence for a system of general protein glycosylation in Campylobacter jejuni [J ] . Molecular Microbiology , 1999 , 32 ( 5 ): 1022 - 1030 .
PARKHILL J , WREN B W , MUNGALL K , et al . The genome sequence of the food-borne pathogen Campylobacter jejuni reveals hypervariable sequences [J ] . Nature , 2000 , 403 ( 6770 ): 665 - 668 .
FELDMAN M F , WACKER M , HERNANDEZ M , et al . Engineering N -linked protein glycosylation with diverse O antigen lipopolysaccharide structures in Escherichia coli [J ] . Proceedings of the National Academy of Sciences of the United States of America , 2005 , 102 ( 8 ): 3016 - 3021 .
BERTANI B , RUIZ N . Function and biogenesis of lipopolysaccharides [J ] . EcoSal Plus , 2018 , 8 ( 1 ): ESP-0001-2018 .
WHITFIELD C , WEAR S S , SANDE C . Assembly of bacterial capsular polysaccharides and exopolysaccharides [J ] . Annual Review of Microbiology , 2020 , 74 : 521 - 543 .
KOWARIK M , YOUNG N M , NUMAO S , et al . Definition of the bacterial N -glycosylation site consensus sequence [J ] . The EMBO Journal , 2006 , 25 ( 9 ): 1957 - 1966 .
IHSSEN J , KOWARIK M , DILETTOSO S , et al . Production of glycoprotein vaccines in Escherichia coli [J ] . Microbial Cell Factories , 2010 , 9 : 61 .
HATZ C F R , BALLY B , ROHRER S , et al . Safety and immunogenicity of a candidate bioconjugate vaccine against Shigella dysenteriae type 1 administered to healthy adults: a single blind, partially randomized Phase Ⅰ study [J ] . Vaccine , 2015 , 33 ( 36 ): 4594 - 4601 .
RAVENSCROFT N , BRAUN M , SCHNEIDER J , et al . Characterization and immunogenicity of a Shigella flexneri 2a O -antigen bioconjugate vaccine candidate [J ] . Glycobiology , 2019 , 29 ( 9 ): 669 - 680 .
VAN DEN DOBBELSTEEN G P J M , FAÉ K C , SERROYEN J , et al . Immunogenicity and safety of a tetravalent E. coli O -antigen bioconjugate vaccine in animal models [J ] . Vaccine , 2016 , 34 ( 35 ): 4152 - 4160 .
MARSHALL L E , NELSON M , DAVIES C H , et al . An O -antigen glycoconjugate vaccine produced using protein glycan coupling technology is protective in an inhalational rat model of tularemia [J ] . Journal of Immunology Research , 2018 , 2018 : 8087916 .
GARCIA-QUINTANILLA F , IWASHKIW J A , PRICE N L , et al . Production of a recombinant vaccine candidate against Burkholderia pseudomallei exploiting the bacterial N -glycosylation machinery [J ] . Frontiers in Microbiology , 2014 , 5 : 381 .
IWASHKIW J A , FENTABIL M A , FARIDMOAYER A , et al . Exploiting the Campylobacter jejuni protein glycosylation system for glycoengineering vaccines and diagnostic tools directed against brucellosis [J ] . Microbial Cell Factories , 2012 , 11 : 13 .
WACKER M , WANG L H , KOWARIK M , et al . Prevention of Staphylococcus aureus infections by glycoprotein vaccines synthesized in Escherichia coli [J ] . The Journal of Infectious Diseases , 2014 , 209 ( 10 ): 1551 - 1561 .
HERBERT J A , KAY E J , FAUSTINI S E , et al . Production and efficacy of a low-cost recombinant pneumococcal protein polysaccharide conjugate vaccine [J ] . Vaccine , 2018 , 36 ( 26 ): 3809 - 3819 .
LIZAK C , GERBER S , NUMAO S , et al . X-ray structure of a bacterial oligosaccharyltransferase [J ] . Nature , 2011 , 474 ( 7351 ): 350 - 355 .
NAPIÓRKOWSKA M , BOILEVIN J , SOVDAT T , et al . Molecular basis of lipid-linked oligosaccharide recognition and processing by bacterial oligosaccharyltransferase [J ] . Nature Structural & Molecular Biology , 2017 , 24 ( 12 ): 1100 - 1106 .
IHSSEN J , HAAS J , KOWARIK M , et al . Increased efficiency of Campylobacter jejuni N -oligosaccharyltransferase PglB by structure-guided engineering [J ] . Open Biology , 2015 , 5 ( 4 ): 140227 .
STIMSON E , VIRJI M , BARKER S , et al . Discovery of a novel protein modification: α-glycerophosphate is a substituent of meningococcal pilin [J ] . Biochemical Journal , 1996 , 316 ( 1 ): 29 - 33 .
FARIDMOAYER A , FENTABIL M A , MILLS D C , et al . Functional characterization of bacterial oligosaccharyltransferases involved in O -linked protein glycosylation [J ] . Journal of Bacteriology , 2007 , 189 ( 22 ): 8088 - 8098 .
FARIDMOAYER A , FENTABIL M A , HAURAT M F , et al . Extreme substrate promiscuity of the Neisseria oligosaccharyl transferase involved in protein O -glycosylation [J ] . Journal of Biological Chemistry , 2008 , 283 ( 50 ): 34596 - 34604 .
PAN C , SUN P , LIU B , et al . Biosynthesis of conjugate vaccines using an O -linked glycosylation system [J ] . mBio , 2016 , 7 ( 2 ): e00443-16 .
SUN P , PAN C , ZENG M , et al . Design and production of conjugate vaccines against S. Paratyphi A using an O -linked glycosylation system in vivo [J ] . NPJ Vaccines , 2018 , 3 : 4 .
LI S L , HUANG J , WANG K F , et al . A bioconjugate vaccine against Brucella abortus produced by engineered Escherichia coli [J ] . Frontiers in Bioengineering and Biotechnology , 2023 , 11 : 1121074 .
LIU Y , PAN C , WANG K F , et al . Preparation of a Klebsiella pneumoniae conjugate nanovaccine using glycol-engineered Escherichia coli [J ] . Microbial Cell Factories , 2023 , 22 ( 1 ): 95 .
SCHULZ B L , JEN F E C , POWER P M , et al . Identification of bacterial protein O -oligosaccharyltransferases and their glycoprotein substrates [J ] . PLoS One , 2013 , 8 ( 5 ): e62768 .
GEBHART C , IELMINI M V , REIZ B , et al . Characterization of exogenous bacterial oligosaccharyltransferases in Escherichia coli reveals the potential for O -linked protein glycosylation in Vibrio cholerae and Burkholderia thailandensis [J ] . Glycobiology , 2012 , 22 ( 7 ): 962 - 974 .
ELHENAWY W , SCOTT N E , TONDO M L , et al . Protein O -linked glycosylation in the plant pathogen Ralstonia solanacearum [J ] . Glycobiology , 2016 , 26 ( 3 ): 301 - 311 .
HADJINEOPHYTOU C , ANONSEN J H , SVINGERUD T , et al . Sculpting the bacterial O -glycoproteome: functional analyses of orthologous oligosaccharyltransferases with diverse targeting specificities [J ] . mBio , 2022 , 13 ( 3 ): e0379721 .
HARDING C M , NASR M A , KINSELLA R L , et al . Acinetobacter strains carry two functional oligosaccharyltransferases, one devoted exclusively to type Ⅳ pilin, and the other one dedicated to O -glycosylation of multiple proteins [J ] . Molecular Microbiology , 2015 , 96 ( 5 ): 1023 - 1041 .
HARDING C M , NASR M A , SCOTT N E , et al . A platform for glycoengineering a polyvalent pneumococcal bioconjugate vaccine using E. coli as a host [J ] . Nature Communications , 2019 , 10 : 891 .
KNOOT C J , ROBINSON L S , HARDING C M . A minimal sequon sufficient for O -linked glycosylation by the versatile oligosaccharyltransferase PglS [J ] . Glycobiology , 2021 , 31 ( 9 ): 1192 - 1203 .
FELDMAN M F , MAYER BRIDWELL A E , SCOTT N E , et al . A promising bioconjugate vaccine against hypervirulent Klebsiella pneumoniae [J ] . Proceedings of the National Academy of Sciences of the United States of America , 2019 , 116 ( 37 ): 18655 - 18663 .
DUKE J A , PASCHALL A V , ROBINSON L S , et al . Development and immunogenicity of a prototype multivalent group B Streptococcus bioconjugate vaccine [J ] . ACS Infectious Diseases , 2021 , 7 ( 11 ): 3111 - 3123 .
KNOOT C J , WANTUCH P L , ROBINSON L S , et al . Discovery and characterization of a new class of O -linking oligosaccharyltransferases from the Moraxellaceae family [J ] . Glycobiology , 2023 , 33 ( 1 ): 57 - 74 .
HARVEY H , KUS J V , TESSIER L , et al . Pseudomonas aeruginosa D-arabinofuranose biosynthetic pathway and its role in type Ⅳ pilus assembly [J ] . Journal of Biological Chemistry , 2011 , 286 ( 32 ): 28128 - 28137 .
HARDING C M , FELDMAN M F . Glycoengineering bioconjugate vaccines, therapeutics, and diagnostics in E. coli [J ] . Glycobiology , 2019 , 29 ( 7 ): 519 - 529 .
ZARSCHLER K , JANESCH B , PABST M , et al . Protein tyrosine O -glycosylation—a rather unexplored prokaryotic glycosylation system [J ] . Glycobiology , 2010 , 20 ( 6 ): 787 - 798 .
MAES E , KRZEWINSKI F , GARENAUX E , et al . Glycosylation of BclA glycoprotein from Bacillus cereus and Bacillus anthracis exosporium is domain-specific [J ] . Journal of Biological Chemistry , 2016 , 291 ( 18 ): 9666 - 9677 .
DEL BINO L , ØSTERLID K E , WU D Y , et al . Synthetic glycans to improve current glycoconjugate vaccines and fight antimicrobial resistance [J ] . Chemical Reviews , 2022 , 122 ( 20 ): 15672 - 15716 .
MICOLI F , COSTANTINO P , ADAMO R . Potential targets for next generation antimicrobial glycoconjugate vaccines [J ] . FEMS Microbiology Reviews , 2018 , 42 ( 3 ): 388 - 423 .
HUANG Y L , WU C Y . Carbohydrate-based vaccines: challenges and opportunities [J ] . Expert Review of Vaccines , 2010 , 9 ( 11 ): 1257 - 1274 .
WILDER-SMITH A . Meningococcal disease: risk for international travellers and vaccine strategies [J ] . Travel Medicine and Infectious Disease , 2008 , 6 ( 4 ): 182 - 186 .
Prevention and control of meningococcal disease: recommendations of the Advisory Committee on Immunization Practices (ACIP) [J/OL ] . MMWR Recommendations and Reports , 2000 , 49 (RR 07 ): 1 - 10 [ 2023-09-01 ] . https://www.cdc.gov/mmwr/preview/mmwrhtml/rr4907a1.htm https://www.cdc.gov/mmwr/preview/mmwrhtml/rr4907a1.htm .
SCHUERMAN L , PRYMULA R , HENCKAERTS I , et al . ELISA IgG concentrations and opsonophagocytic activity following pneumococcal protein D conjugate vaccination and relationship to efficacy against acute otitis media [J ] . Vaccine , 2007 , 25 ( 11 ): 1962 - 1968 .
PRYMULA R , PEETERS P , CHROBOK V , et al . Pneumococcal capsular polysaccharides conjugated to protein D for prevention of acute otitis media caused by both Streptococcus pneumoniae and non-typable Haemophilus influenzae : a randomised double-blind efficacy study [J ] . The Lancet , 2006 , 367 ( 9512 ): 740 - 748 .
ZHU H , ROLLIER C S , POLLARD A J . Recent advances in lipopolysaccharide-based glycoconjugate vaccines [J ] . Expert Review of Vaccines , 2021 , 20 ( 12 ): 1515 - 1538 .
COHEN D , ATSMON J , ARTAUD C , et al . Safety and immunogenicity of a synthetic carbohydrate conjugate vaccine against Shigella flexneri 2a in healthy adult volunteers: a phase 1, dose-escalating, single-blind, randomised, placebo-controlled study [J ] . The Lancet Infectious Diseases , 2021 , 21 ( 4 ): 546 - 558 .
FRENCK R W JR , ERVIN J , CHU L , et al . Safety and immunogenicity of a vaccine for extra-intestinal pathogenic Escherichia coli (ESTELLA): a phase 2 randomised controlled trial [J ] . The Lancet Infectious Diseases , 2019 , 19 ( 6 ): 631 - 640 .
DAVID S , REUTER S , HARRIS S R , et al . Epidemic of carbapenem-resistant Klebsiella pneumoniae in Europe is driven by nosocomial spread [J ] . Nature Microbiology , 2019 , 4 ( 11 ): 1919 - 1929 .
SHEN X , LAGERGÅRD T , YANG Y , et al . Group B Streptococcus capsular polysaccharide-cholera toxin B subunit conjugate vaccines prepared by different methods for intranasal immunization [J ] . Infection and Immunity , 2001 , 69 ( 1 ): 297 - 306 .
DOW J M , MAURI M , SCOTT T A , et al . Improving protein glycan coupling technology (PGCT) for glycoconjugate vaccine production [J ] . Expert Review of Vaccines , 2020 , 19 ( 6 ): 507 - 527 .
ROMANO M R , LEUZZI R , CAPPELLETTI E , et al . Recombinant Clostridium difficile toxin fragments as carrier protein for PSⅡ surface polysaccharide preserve their neutralizing activity [J ] . Toxins , 2014 , 6 ( 4 ): 1385 - 1396 .
NILO A , PASSALACQUA I , FABBRINI M , et al . Exploring the effect of conjugation site and chemistry on the immunogenicity of an anti-group B streptococcus glycoconjugate vaccine based on GBS67 pilus protein and type Ⅴ polysaccharide [J ] . Bioconjugate Chemistry , 2015 , 26 ( 8 ): 1839 - 1849 .
YUE H , MA G H . Polymeric micro/nanoparticles: particle design and potential vaccine delivery applications [J ] . Vaccine , 2015 , 33 ( 44 ): 5927 - 5936 .
NEEK M , KIM T I , WANG S W . Protein-based nanoparticles in cancer vaccine development [J ] . Nanomedicine: Nanotechnology, Biology, and Medicine , 2019 , 15 ( 1 ): 164 - 174 .
CHEN J Y , WANG P , YUAN L Z , et al . A live attenuated virus-based intranasal COVID-19 vaccine provides rapid, prolonged, and broad protection against SARS-CoV-2 [J ] . Science Bulletin , 2022 , 67 ( 13 ): 1372 - 1387 .
CHARLTON HUME H K , VIDIGAL J , CARRONDO M J T , et al . Synthetic biology for bioengineering virus-like particle vaccines [J ] . Biotechnology and Bioengineering , 2019 , 116 ( 4 ): 919 - 935 .
LI X , PAN C , SUN P , et al . Orthogonal modular biosynthesis of nanoscale conjugate vaccines for vaccination against infection [J ] . Nano Research , 2022 , 15 ( 2 ): 1645 - 1653 .
PAN C , WU J , QING S , et al . Biosynthesis of self-assembled proteinaceous nanoparticles for vaccination [J ] . Advanced Materials , 2020 , 32 ( 42 ): e2002940 .
HUANG B , XU Y , HU X H , et al . A backbone-centred energy function of neural networks for protein design [J ] . Nature , 2022 , 602 ( 7897 ): 523 - 528 .
PENG Z H , WU J , WANG K F , et al . Production of a promising biosynthetic self-assembled nanoconjugate vaccine against Klebsiella pneumoniae serotype O 2 in a general Escherichia coli host [J ] . Advanced Science , 2021 , 8 ( 14 ): e2100549 .
SHI Y X , PAN C , WANG K F , et al . Construction of orthogonal modular proteinaceous nanovaccine delivery vectors based on mSA-biotin binding [J ] . Nanomaterials , 2022 , 12 ( 5 ): 734 .
TEMME K , ZHAO D H , VOIGT C A . Refactoring the nitrogen fixation gene cluster from Klebsiella oxytoca [J ] . Proceedings of the National Academy of Sciences of the United States of America , 2012 , 109 ( 18 ): 7085 - 7090 .
SMANSKI M J , BHATIA S , ZHAO D H , et al . Functional optimization of gene clusters by combinatorial design and assembly [J ] . Nature Biotechnology , 2014 , 32 ( 12 ): 1241 - 1249 .
ZELCBUCH L , ANTONOVSKY N , BAR-EVEN A , et al . Spanning high-dimensional expression space using ribosome-binding site combinatorics [J ] . Nucleic Acids Research , 2013 , 41 ( 9 ): e98 .
TAYLOR G M , MORDAKA P M , HEAP J T . Start-Stop Assembly: a functionally scarless DNA assembly system optimized for metabolic engineering [J ] . Nucleic Acids Research , 2019 , 47 ( 3 ): e17 .
KAY E J , MAURI M , WILLCOCKS S J , et al . Engineering a suite of E. coli strains for enhanced expression of bacterial polysaccharides and glycoconjugate vaccines [J ] . Microbial Cell Factories , 2022 , 21 ( 1 ): 66 .
GASPERINI G , RASO M M , SCHIAVO F , et al . Rapid generation of Shigella flexneri GMMA displaying natural or new and cross-reactive O -Antigens [J ] . NPJ Vaccines , 2022 , 7 : 69 .
JAROENTOMEECHAI T , KWON Y H , LIU Y W , et al . A universal glycoenzyme biosynthesis pipeline that enables efficient cell-free remodeling of glycans [J ] . Nature Communications , 2022 , 13 : 6325 .
GLASSCOCK C J , YATES L E , JAROENTOMEECHAI T , et al . A flow cytometric approach to engineering Escherichia coli for improved eukaryotic protein glycosylation [J ] . Metabolic Engineering , 2018 , 47 : 488 - 495 .
CERONI F , ALGAR R , STAN G B , et al . Quantifying cellular capacity identifies gene expression designs with reduced burden [J ] . Nature Methods , 2015 , 12 ( 5 ): 415 - 418 .
LINTON D , DORRELL N , HITCHEN P G , et al . Functional analysis of the Campylobacter jejuni N -linked protein glycosylation pathway [J ] . Molecular Microbiology , 2005 , 55 ( 6 ): 1695 - 1703 .
NEUHARD J , THOMASSEN E . Altered deoxyribonucleotide pools in P2 eductants of Escherichia coli K-12 due to deletion of the dcd gene [J ] . Journal of Bacteriology , 1976 , 126 ( 2 ): 999 - 1001 .
ALAIMO C , CATREIN I , MORF L , et al . Two distinct but interchangeable mechanisms for flipping of lipid-linked oligosaccharides [J ] . The EMBO Journal , 2006 , 25 ( 5 ): 967 - 976 .
PÉREZ J M , MCGARRY M A , MAROLDA C L , et al . Functional analysis of the large periplasmic loop of the Escherichia coli K-12 WaaL O -antigen ligase [J ] . Molecular Microbiology , 2008 , 70 ( 6 ): 1424 - 1440 .
MUSUMECI M A , FARIDMOAYER A , WATANABE Y , et al . Evaluating the role of conserved amino acids in bacterial O -oligosaccharyltransferases by in vivo , in vitro and limited proteolysis assays [J ] . Glycobiology , 2014 , 24 ( 1 ): 39 - 50 .
STRUTTON B , JAFFÉ S R P , PANDHAL J , et al . Producing a glycosylating Escherichia coli cell factory: the placement of the bacterial oligosaccharyl transferase pglB onto the genome [J ] . Biochemical and Biophysical Research Communications , 2018 , 495 ( 1 ): 686 - 692 .
YATES L E , NATARAJAN A , LI M J , et al . Glyco-recoded Escherichia coli : recombineering-based genome editing of native polysaccharide biosynthesis gene clusters [J ] . Metabolic Engineering , 2019 , 53 : 59 - 68 .
NATARAJAN A , JAROENTOMEECHAI T , LI M J , et al . Metabolic engineering of glycoprotein biosynthesis in bacteria [J ] . Emerging Topics in Life Sciences , 2018 , 2 ( 3 ): 419 - 432 .
YU H , CHEN X . One-pot multienzyme (OPME) systems for chemoenzymatic synthesis of carbohydrates [J ] . Organic & Biomolecular Chemistry , 2016 , 14 ( 10 ): 2809 - 2818 .
GUARINO C , DELISA M P . A prokaryote-based cell-free translation system that efficiently synthesizes glycoproteins [J ] . Glycobiology , 2012 , 22 ( 5 ): 596 - 601 .
SHIMIZU Y , INOUE A , TOMARI Y , et al . Cell-free translation reconstituted with purified components [J ] . Nature Biotechnology , 2001 , 19 ( 8 ): 751 - 755 .
STARK J C , JAROENTOMEECHAI T , MOELLER T D , et al . On-demand, cell-free biomanufacturing of conjugate vaccines at the point-of-care [EB/OL ] . bioRxiv , 2019 : 681841 [ 2023-09-01 ] . https://www.biorxiv.org/content/10.1101/681841v1 https://www.biorxiv.org/content/10.1101/681841v1 .
NATARAJAN A , JAROENTOMEECHAI T , CABRERA-SÁNCHEZ M , et al . Engineering orthogonal human O -linked glycoprotein biosynthesis in bacteria [J ] . Nature Chemical Biology , 2020 , 16 ( 10 ): 1062 - 1070 .
0
Views
1
下载量
2
CSCD
Publicity Resources
Related Articles
Related Author
Related Institution
京公网安备11010802024621