Progress in synthetic biology research of <italic style="font-style: italic">Clostridium thermocellum</italic> for biomass energy applications

XIAO Yan; LIU Yajun; FENG Yin′gang; CUI Qiu

doi:10.12211/2096-8280.2023-046

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Progress in synthetic biology research of Clostridium thermocellum for biomass energy applications

Invited Review | 更新时间：2025-03-20

- Progress in synthetic biology research of Clostridium thermocellum for biomass energy applications
- Synthetic Biology Journal Vol. 4, Issue 6, Pages: 1055-1081(2023)
- 作者机构：
  
  1.中国科学院青岛生物能源与过程研究所，中国科学院生物燃料重点实验室，山东省合成生物学重点实验室，山东青岛 266101
  2.山东省能源研究院，山东青岛 266101
  3.青岛新能源山东省实验室，山东青岛 266101
  4.中国科学院大学，北京 100049
- 作者简介：
- 基金信息：
- DOI：10.12211/2096-8280.2023-046
  CLC： Q812
- Received：02 July 2023，
  
  Revised：2023-09-22，
  
  Published：31 December 2023
- 稿件说明：
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肖艳，刘亚君，冯银刚，崔球. 热纤梭菌在生物质能源开发中的合成生物学研究进展［J］. 合成生物学， 2023， 4（6）： 1055-1081

XIAO Yan， LIU Yajun， FENG Yin′gang， CUI Qiu. Progress in synthetic biology research of Clostridium thermocellum for biomass energy applications［J］. Synthetic Biology Journal， 2023， 4（6）： 1055-1081
肖艳，刘亚君，冯银刚，崔球. 热纤梭菌在生物质能源开发中的合成生物学研究进展［J］. 合成生物学， 2023， 4（6）： 1055-1081 DOI： 10.12211/2096-8280.2023-046.

XIAO Yan， LIU Yajun， FENG Yin′gang， CUI Qiu. Progress in synthetic biology research of Clostridium thermocellum for biomass energy applications［J］. Synthetic Biology Journal， 2023， 4（6）： 1055-1081 DOI： 10.12211/2096-8280.2023-046.

摘要

农林废弃物、能源植物、微藻等生物质是唯一同时具备“能源”和“物质”双重属性的可再生资源，在替代不可再生的化石能源方面具有巨大的潜力。木质纤维素生物转化的核心之一在于高效生物催化剂的构建。热纤梭菌是高效降解木质纤维素的嗜热厌氧菌，是多种木质纤维素生物转化策略的理想底盘菌株，在生物质能源开发中具有重要价值。经过近二十年的研究和开发，针对热纤梭菌已经建立了多种遗传改造技术，并构建了可以生产多种能源分子及化学品的热纤梭菌细胞工厂。本文首先介绍了热纤梭菌及其纤维素降解与利用特性，简述了热纤梭菌的系统生物学研究和遗传改造工具开发的现状，随后重点回顾和总结了热纤梭菌在生产乙醇、丁醇、异丁醇、氢气、乳酸、中/短链脂肪酸酯和可发酵糖等生物能源开发中的合成生物学研究进展。最后对热纤梭菌的合成生物学发展方向进行了展望，并强调了合成生物学技术在未来生物质能源开发中的重要作用。

Abstract

Biomass

including agricultural and forestry waste

energy plants

and microalgae

possesses both "energy" and "substance" properties

making it a promising renewable resource that can potentially replace fossil fuels. The efficient lignocellulose bioconversion relies on the development of effective biocatalysts.

Clostridium thermocellum

(also known as

Ruminiclostridium thermocellum

Hungateiclostridium thermocellum

and

Acetivibrio thermocellus

) is a thermophilic anaerobic bacterium that can efficiently degrade lignocellulosic biomass. Over the past two decades

extensive research and development have led to the potential of using

C. thermocellum

as a cell factory to produce various energy and chemicals from lignocellulose.

C. thermocellum

has been used to produce ethanol

butanol

isobutanol

hydrogen

lactic acid

medium/short-chain fatty acid esters

and fermentable sugars from lignocellulosic biomass. The degradation and utilization process of lignocellulosic biomass by

C. thermocellum

mainly involves substrate recognition and hydrolysis through the cellulosome

hydrolysate uptake through ABC transporters

and intracellular metabolism

via

atypical glycolytic pathways.

C. thermocellum

possesses dynamic regulation of cellulosome production adapting extracellular substrates

which enables the

high capability of degrading various lignocellulosic substrates. The cellulosome consists of non-catalytic scaffoldins and multiple enzymatic subunits with distinct catalytic activities and has broad applications in synthetic biology as well as lignocellulose degradation. In addition to lignocellulose refinery

the thermophilic

C. thermocellum

also has great potential in synthetic biology research under high-temperature conditions. Several genetic manipulation tools have been developed for

C. thermocellum

although greater challenges have been encountered compared to model organisms such as

Escherichia coli

. The genetic tools include homologous recombination technology

Thermotargetron technology

and CRISPR/Cas systems

which enable gene knockout

insertion

replacement

mutation

and expression regulation of target genes in the strain.

C. thermocellum

has been used as the whole-cell biocatalyst for lignocellulose bioconversion through consolidated bioprocessing (CBP) and consolidated bio-saccharification (CBS). CBS follows the concept of sugar platform construction and shows great potential in real-world applications. The synthetic biology research targeting the CBS strategy still requires future development. For example

we need to explore new genetic tools and thermophilic functional elements for

C. thermocellum

and improve the efficiency of gene editing. We need to st

rengthen research on the genetic

physiological

and metabolic aspects of

C. thermocellum

and the molecular mechanisms underlying lignocellulose degradation. It is noteworthy that

as a strict anaerobe

C. thermocellum

cannot be used as the chassis for catalyzing oxygen-involved reactions. Selecting suitable metabolic pathways and target products will be the focus in future developments of synthetic biology based on

C. thermocellum

. Therefore

we need to investigate additional target pathways and products for synthetic biology development. In recent years

automation methods and artificial intelligence (AI) technologies are being developed rapidly and have been applied in various synthetic biology research fields. Such technologies may also be employed to promote research on thermophilic and anaerobic microorganisms.

关键词

Keywords

references

LI F H , LI Y W , NOVOSELOV K S , et al . Bioresource upgrade for sustainable energy, environment, and biomedicine [J ] . Nano-Micro Letters , 2023 , 15 ( 1 ): 35 .

LYND L R , BECKHAM G T , GUSS A M , et al . Toward low-cost biological and hybrid biological/catalytic conversion of cellulosic biomass to fuels [J ] . Energy & Environmental Science , 2022 , 15 ( 3 ): 938 - 990 .

KEASLING J , GARCIA MARTIN H , LEE T S , et al . Microbial production of advanced biofuels [J ] . Nature Reviews Microbiology , 2021 , 19 ( 11 ): 701 - 715 .

LIU Y J , LI B , FENG Y G , et al . Consolidated bio-saccharification: leading lignocellulose bioconversion into the real world [J ] . Biotechnology Advances , 2020 , 40 : 107535 .

ZHANG H Y , HAN L J , DONG H M . An insight to pretreatment, enzyme adsorption and enzymatic hydrolysis of lignocellulosic biomass: experimental and modeling studies [J ] . Renewable and Sustainable Energy Reviews , 2021 , 140 : 110758 .

RE A , MAZZOLI R . Current progress on engineering microbial strains and consortia for production of cellulosic butanol through consolidated bioprocessing [J ] . Microbial Biotechnology , 2023 , 16 ( 2 ): 238 - 261 .

LYND L R , VAN ZYL W H , MCBRIDE J E , et al . Consolidated bioprocessing of cellulosic biomass: an update [J ] . Current Opinion in Biotechnology , 2005 , 16 ( 5 ): 577 - 583 .

LIN P P , MI L , MORIOKA A H , et al . Consolidated bioprocessing of cellulose to isobutanol using Clostridium thermocellum [J ] . Metabolic Engineering , 2015 , 31 : 44 - 52 .

KOTHARI N , BHAGIA S , PU Y Q , et al . The effect of switchgrass plant cell wall properties on its deconstruction by thermochemical pretreatments coupled with fungal enzymatic hydrolysis or Clostridium thermocellum consolidated bioprocessing [J ] . Green Chemistry , 2020 , 22 ( 22 ): 7924 - 7945 .

PERIYASAMY S , BEULA ISABEL J , KAVITHA S , et al . Recent advances in consolidated bioprocessing for conversion of lignocellulosic biomass into bioethanol — a review [J ] . Chemical Engineering Journal , 2023 , 453 : 139783 .

DEMAIN A L , NEWCOMB M , DAVID WU J H . Cellulase, Clostridia , and ethanol [J ] . Microbiology and Molecular Biology Reviews , 2005 , 69 ( 1 ): 124 - 154 .

OLSON D G , HÖRL M , FUHRER T , et al . Glycolysis without pyruvate kinase in Clostridium thermocellum [J ] . Metabolic Engineering , 2017 , 39 : 169 - 180 .

LAMED R , BAYER E A . Cellulosomes from Clostridium thermocellum [J ] . Methods in Enzymology , 1988 , 160 : 472 - 482 .

HOLWERDA E K , WORTHEN R S , KOTHARI N , et al . Multiple levers for overcoming the recalcitrance of lignocellulosic biomass [J ] . Biotechnology for Biofuels , 2019 , 12 : 15 .

ARTZI L , BAYER E A , MORAÏS S . Cellulosomes: bacterial nanomachines for dismantling plant polysaccharides [J ] . Nature Reviews Microbiology , 2017 , 15 ( 2 ): 83 - 95 .

ZHANG Y H P , LYND L R . Cellulose utilization by Clostridium thermocellum : bioenergetics and hydrolysis product assimilation [J ] . Proceedings of the National Academy of Sciences of the United States of America , 2005 , 102 ( 20 ): 7321 - 7325 .

YAN F , DONG S , LIU Y J , et al . Deciphering cellodextrin and glucose uptake in Clostridium thermocellum [J ] . mBio , 2022 , 13 ( 5 ): e01476-22 .

MAZZOLI R , OLSON D G . Clostridium thermocellum : a microbial platform for high-value chemical production from lignocellulose [M/OL ] // Advances in Applied Microbiology . Amsterdam : Elsevier , 2020 : 111 - 161 [2023-06-01] . https://www.sciencedirect.com/science/article/abs/pii/S0065216420300423?via%3Dihub https://www.sciencedirect.com/science/article/abs/pii/S0065216420300423?via%3Dihub .

LIU Y J , ZHANG Y D , CHI F , et al . Integrated lactic acid production from lignocellulosic agricultural wastes under thermal conditions [J ] . Journal of Environmental Management , 2023 , 342 : 118281 .

LIU G L , BU X Y , CHEN C Y , et al . Bioconversion of non-food corn biomass to polyol esters of fatty acid and single-cell oils [J ] . Biotechnology for Biofuels and Bioproducts , 2023 , 16 ( 1 ): 9 .

LIU G L , ZHAO X X , CHEN C , et al . Robust production of pigment-free pullulan from lignocellulosic hydrolysate by a new fungus co-utilizing glucose and xylose [J ] . Carbohydrate Polymers , 2020 , 241 : 116400 .

LÓPEZ-MONDÉJAR R , ALGORA C , BALDRIAN P . Lignocellulolytic systems of soil bacteria: a vast and diverse toolbox for biotechnological conversion processes [J ] . Biotechnology Advances , 2019 , 37 ( 6 ): 107374 .

XIONG W , REYES L H , MICHENER W E , et al . Engineering cellulolytic bacterium Clostridium thermocellum to co-ferment cellulose- and hemicellulose-derived sugars simultaneously [J ] . Biotechnology and Bioengineering , 2018 , 115 ( 7 ): 1755 - 1763 .

JOHNSON E A , REESE E T , DEMAIN A . Inhibition of Clostridium thermocellum cellulase by end products of cellulolysis [J ] . Journal of Applied Biochemistry , 1982 , 4 : 64 - 71 .

BROWN S D , GUSS A M , KARPINETS T V , et al . Mutant alcohol dehydrogenase leads to improved ethanol tolerance in Clostridium thermocellum [J ] . Proceedings of the National Academy of Sciences of the United States of America , 2011 , 108 ( 33 ): 13752 - 13757 .

HAMILTON-BREHM S D , MOSHER J J , VISHNIVETSKAYA T , et al . Caldicellulosiruptor obsidiansis sp. nov., an anaerobic, extremely thermophilic, cellulolytic bacterium isolated from obsidian pool, Yellowstone National Park [J ] . Applied and Environmental Microbiology , 2010 , 76 ( 4 ): 1014 - 1020 .

DESVAUX M , GUEDON E , PETITDEMANGE H . Cellulose catabol ism by Clostridium cellulolyticum growing in batch culture on defined medium [J ] . Applied and Environmental Microbiology , 2000 , 66 ( 6 ): 2461 - 2470 .

TINDALL B J . The names Hungateiclostridium Zhang et al. 2018 , Hungateiclostridium thermocellum (Viljoenet al. 1926 ) Zhanget al. 2018, Hungateiclostridium cellulolyticum (Patelet al. 1980) Zhanget al. 2018, Hungateiclostridium aldrichii (Yanget al. 1990) Zhanget al. 2018, Hungateiclostridium alkalicellulosi (Zhilinaet al. 2006) Zhanget al. 2018, Hungateiclostridium clariflavum (Shiratoriet al. 2009) Zhanget al. 2018, Hungateiclostridium straminisolvens (Katoet al. 2004) Zhang et al. 2018 and Hungateiclostridium saccincola (Koecket al. 2016) Zhanget al. 2018 contravene Rule 51b of the International Code of Nomenclature of Prokaryotes and require replacement names in the genus Acetivibrio Patelet al. 1980 [J/OL ] . International Journal of Systematic and Evolutionary Microbiology, 2019, 69 ( 12 ): 3927 - 3932 [ 2023-06-01 ] . https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.003685 https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.003685 .

AKINOSHO H , YEE K , CLOSE D , et al . The emergence of Clostridium thermocellum as a high utility candidate for consolidated bioprocessing applications [J ] . Frontiers in Chemistry , 2014 , 2 : 66 .

FEINBERG L , FODEN J , BARRETT T , et al . Complete genome sequence of the cellulolytic thermophile Clostridium thermocellum DSM 1313 [J ] . Journal of Bacteriology , 2011 , 193 ( 11 ): 2906 - 2907 .

TRIPATHI S A , OLSON D G , ARGYROS D A , et al . Development of pyrF -based genetic system for targeted gene deletion in Clostridium thermocellum and creation of a pta mutant [J ] . Applied and Environmental Microbiology , 2010 , 76 ( 19 ): 6591 - 6599 .

OLSON D G , LYND L R . Transformation of Clostridium thermocellum by electroporation [M/OL ] . Methods in enzymology , 2012 , 510: 317 - 330 [ 2023-06-01 ] . https://www.sciencedirect.com/science/article/abs/pii/B9780124159310000173?via%3Dihub https://www.sciencedirect.com/science/article/abs/pii/B9780124159310000173?via%3Dihub .

RILEY L A , JI L X , SCHMITZ R J , et al . Rational development of transformation in Clostridium thermocellum ATCC 27405 via complete methylome analysis and evasion of native restriction-modification systems [J ] . Journal of Industrial Microbiology and Biotechnology , 2019 , 46 ( 9/10 ): 1435 - 1443 .

BAYER E A , BELAICH J P , SHOHAM Y , et al . The cellulosomes: multienzyme machines for degradation of plant cell wall polysaccharides [J ] . Annual Review of Microbiology , 2004 , 58 : 521 - 554 .

HONG W , ZHANG J , FENG Y G , et al . The contribution of cellulosomal scaffoldins to cellulose hydrolysis by Clostridium thermocellum analyzed by using thermotargetrons [J ] . Biotechnology for Biofuels , 2014 , 7 : 80 .

冯银刚 , 刘亚君 , 崔球 . 纤维小体在合成生物学中的应用研究进展 [J ] . 合成生物学 , 2022 , 3 ( 1 ): 138 - 154 .

FENG Y G , LIU Y J , CUI Q . Research progress in cellulosomes and their applications in synthetic biology [J ] . Synthetic Biology Journal , 2022 , 3 ( 1 ): 138 - 154 .

ZVERLOV V V , KELLERMANN J , SCHWARZ W H . Functional subgenomics of Clostridium thermocellum cellulosomal genes: identification of the major catalytic components in the extracellular complex and detection of three new enzymes [J ] . Proteomics , 2005 , 5 ( 14 ): 3646 - 3653 .

KUROKAWA J , HEMJINDA E , ARAI T , et al . Clostridium thermocellum cellulase CelT, a family 9 endoglucanase without an Ig-like domain or family 3c carbohydrate-binding module [J ] . Applied Microbiology and Biotechnology , 2002 , 59 ( 4 ): 455 - 461 .

ZVERLOV V V , SCHANTZ N , SCHWARZ W H . A major new component in the cellulosome of Clostridium thermocellum is a processive endo-β-1, 4-glucanase producing cellotetraose [J ] . FEMS Microbiology Letters , 2005 , 249 ( 2 ): 353 - 358 .

YE X H , ZHU Z G , ZHANG C M , et al . Fusion of a family 9 cellulose-binding module improves catalytic potential of Clostridium thermocellum cellodextrin phosphorylase on insoluble cellulose [J ] . Applied Microbiology and Biotechnology , 2011 , 92 ( 3 ): 551 - 560 .

ANBAR M , GUL O , LAMED R , et al . Improved thermostability of Clostridium thermocellum endoglucanase Cel8A by using consensus-guided mutagenesis [J ] . Applied and Environmental Microbiology , 2012 , 78 ( 9 ): 3458 - 3464 .

LEIS B , HELD C , BERGKEMPER F , et al . Comparative characterization of all cellulosomal cellulases from Clostridium thermocellum reveals high diversity in endoglucanase product formation essential for complex activity [J ] . Biotechnology for Biofuels , 2017 , 10 : 240 .

YUAN S F , WU T H , LEE H L , et al . Biochemical characterization and structural analysis of a bifunctional cellulase/xylanase from Clostridium thermocellum [J ] . The Journal of Biological Chemistry , 2015 , 290 ( 9 ): 5739 - 5748 .

OLSON D G , TRIPATHI S A , GIANNONE R J , et al . Deletion of the Cel48S cellulase from Clostridium thermocellum [J ] . Proceedings of the National Academy of Sciences of the United States of America , 2010 , 107 ( 41 ): 17727 - 17732 .

LIU Y J , LIU S Y , DONG S , et al . Determination of the native features of the exoglucanase Cel48S from Clostridium thermocellum [J ] . Biotechnology for Biofuels , 2018 , 11 : 6 .

EIBINGER M , GANNER T , PLANK H , et al . A biological nanomachine at work: watching the cellulosome degrade crystalline cellulose [J ] . ACS Central Science , 2020 , 6 ( 5 ): 739 - 746 .

DING S Y , BAYER E A . Understanding cellulosome interaction with cellulose by high-resolution imaging [J ] . ACS Central Science , 2020 , 6 ( 7 ): 1034 - 1036 .

WEI Z , CHEN C , LIU Y J , et al . Alternative σ I /anti-σ I factors represent a unique form of bacterial σ/anti-σ complex [J ] . Nucleic Acids Research , 2019 , 47 ( 11 ): 5988 - 5997 .

SMITH S P , BAYER E A . Insights into cellulosome assembly and dynamics: from dissection to reconstruction of the supramolecular enzyme complex [J ] . Current Opinion in Structural Biology , 2013 , 23 ( 5 ): 686 - 694 .

NATAF Y , BAHARI L , KAHEL-RAIFER H , et al . Clostridium thermocellum cellulosomal g enes are regulated by extracytoplasmic polysaccharides via alternative σ factors [J ] . Proceedings of the National Academy of Sciences of the United States of America , 2010 , 107 ( 43 ): 18646 - 18651 .

STEVENSON D M , WEIMER P J . Expression of 17 genes in Clostridium thermocellum ATCC 27405 during fermentation of cellulose or cellobiose in continuous culture [J ] . Applied and Environmental Microbiology , 2005 , 71 ( 8 ): 4672 - 4678 .

KAHEL-RAIFER H , JINDOU S , BAHARI L , et al . The unique set of putative membrane-associated anti-σ factors in Clostridium thermocellum suggests a novel extracellular carbohydrate-sensing mechanism involved in gene regulation [J ] . FEMS Microbiology Letters , 2010 , 308 ( 1 ): 84 - 93 .

GRINBERG I R , YANIV O , DE ORA L O , et al . Distinctive ligand-binding specificities of tandem PA14 biomass-sensory elements from Clostridium thermocellum and Clostridium clariflavum [J ] . Proteins: Structure, Function, and Bioinformatics , 2019 , 87 ( 11 ): 917 - 930 .

ORTIZ DE ORA L , LAMED R , LIU Y J , et al . Regulation of biomass degradation by alternative σ factors in cellulolytic clostridia [J ] . Scientific Reports , 2018 , 8 : 11036 .

CHEN C , DONG S , YU Z L , et al . Essential autoproteolysis of bacterial anti-σ factor RsgI for transmembrane signal transduction [J ] . Science Advances , 2023 , 9 ( 27 ): eadg4846 .

NATAF Y , YARON S , STAHL F , et al . Cellodextrin and laminaribiose ABC transporters in Clostridium thermocellum [J ] . Journal of Bacteriology , 2009 , 191 ( 1 ): 203 - 209 .

JACOBSON T B , KOROSH T K , STEVENSON D M , et al . In vivo thermodynamic analysis of glycolysis in Clostridium thermocellum and Thermoanaerobacterium saccharolyticum using 13 C and 2 H tracers [J ] . mSystems , 2020 , 5 ( 2 ): e00736 - e19 .

DENG Y , OLSON D G , ZHOU J L , et al . Redirecting carbon flux through exogenous pyruvate kinase to achieve high ethanol yields in Clostridium thermocellum [J ] . Metabolic Engineering , 2013 , 15 : 151 - 158 .

XIONG W , LIN P P , MAGNUSSON L , et al . CO 2 -fixing one-carbon metabolism in a cellulose-degrading bacterium Clostridium thermocellum [J ] . Proceedings of the National Academy of Sciences of the United States of America , 2016 , 113 ( 46 ): 13180 - 13185 .

BROWN S D , RAMAN B , MCKEOWN C K , et al . Construction and evaluation of a Clostridium thermocellum ATCC 27405 whole-genome oligonucleotide microarray [J ] . Applied Biochemistry and Biotechnology , 2007 , 137 : 663 - 674 .

GOLD N D , MARTIN V J J . Global view of the Clostridium thermocellum cellulosome revealed by quantitative proteomic analysis [J ] . Journal of Bacteriology , 2007 , 189 ( 19 ): 6787 - 6795 .

ROBERTS S B , GOWEN C M , BROOKS J P , et al . Genome-scale metabolic analysis of Clostridium thermocellum for bioethanol production [J ] . BMC Systems Biology , 2010 , 4 : 31 .

RIEDERER A , TAKASUKA T E , MAKINO S I , et al . Global gene expression patterns in Clostridium thermocellum as as determined by microarray analysis of chemostat cultures on cellulose or cellobiose [J ] . Applied and Environmental Microbiology , 2011 , 77 ( 4 ): 1243 - 1253 .

WILSON C M , RODRIGUEZ M , JOHNSON C M , et al . Global transcriptome analysis of Clostridium thermocellum ATCC 27405 during growth on dilute acid pretreated Populus and switchgrass [J ] . Biotechnology for Biofuels , 2013 , 6 : 179 .

HOLWERDA E K , THORNE P G , OLSON D G , et al . The exometabolome of Clostridium thermocellum reveals overflow metabolism at high cellulose loading [J ] . Biotechnology for Biofuels , 2014 , 7 : 155 .

CHOU W C , MA Q , YANG S H , et al . Analysis of strand-specific RNA-seq data using machine learning reveals the structures of transcription units in Clostridium thermocellum [J ] . Nucleic Acids Research , 2015 , 43 ( 10 ): e67 .

XU Q , RESCH M G , PODKAMINER K , et al . Dramatic performance of Clostridium thermocellum explained by its wide range of cellulase modalities [J ] . Science Advances , 2016 , 2 ( 2 ): e1501254 .

POUDEL S , GIANNONE R J , RODRIGUEZ M , et al . Integrated omics analyses reveal the details of metabolic adaptation of Clostridium thermocellum to lignocellulose-derived growth inhibitors released during the deconstruction of switchgrass [J ] . Biotechnology for Biofuels , 2017 , 10 : 14 .

DASH S , KHODAYARI A , ZHOU J L , et al . Development of a core Clostridium thermocellum kinetic metabolic model consistent with multiple genetic perturbations [J ] . Biotechnology for Biofuels , 2017 , 10 : 108 .

DUMITRACHE A , KLINGEMAN D M , NATZKE J , et al . Specialized activities and expression differences for Clostridium thermocellum biofilm and planktonic cells [J ] . Scientific Reports , 2017 , 7 : 43583 .

WHITHAM J M , MOON J W , RODRIGUEZ M JR , et al . Clostridium thermocellum LL 1210 pH homeostasis mechanisms informed by transcriptomics and metabolomics [J ] . Biotechnology for Biofuels , 2018 , 11 : 98 .

TAFUR RANGEL A E , CROFT T , GONZÁLEZ BARRIOS A F , et al . Transcriptomic analysis of a Clostridium thermocellum strain engineered to utilize xylose: responses to xylose versus cellobiose feeding [J ] . Scientific Reports , 2020 , 10 : 14517 .

FOSTER C , BOORLA V S , DASH S , et al . Assessing the impact of substrate-level enzyme regulations limiting ethanol titer in Clostridium thermocellum using a core kinetic model [J ] . Metabolic Engineering , 2022 , 69 : 286 - 301 .

SCHROEDER W L , KUIL T , VAN MARIS A J A , et al . A detailed genome-scale metabolic model of Clostridium thermocellum investigates sources of pyrophosphate for driving glycolysis [J ] . Metabolic Engineering , 2023 , 77 : 306 - 322 .

RAMAN B , PAN C L , HURST G B , et al . Impact of pretreated switchgrass and biomass carbohydrates on Clostridium thermocellum ATCC 27405 cellulosome composition: a quantitative proteomic analysis [J ] . PLoS One , 2009 , 4 ( 4 ): e5271 .

BURTON E , MARTIN V J J . Proteomic analysis of Clostridium thermocellum ATCC 27405 reveals the upregulation of an alternative transhydrogenase-malate pathway and nitrogen assimilation in cells grown on cellulose [J ] . Canadian Journal of Microbiology , 2012 , 58 ( 12 ): 1378 - 1388 .

WEI H , FU Y , MAGNUSSON L , et al . Comparison of transcriptional profiles of Clostridium thermocellum grown on cellobiose and pretreated yellow poplar u sing RNA-Seq [J ] . Frontiers in Microbiology , 2014 , 5 : 142 .

YOAV S , BARAK Y , SHAMSHOUM M , et al . How does cellulosome composition influence deconstruction of lignocellulosic substrates in Clostridium ( Ruminiclostridium ) thermocellum DSM 1313? [J ] . Biotechnology for Biofuels , 2017 , 10 : 222 .

DE CAMARGO B R , STEINDORFF A S , DA SILVA L A , et al . Expression profiling of Clostridium thermocellum B8 during the deconstruction of sugarcane bagasse and straw [J ] . World Journal of Microbiology and Biotechnology , 2023 , 39 ( 4 ): 105 .

RAMAN B , MCKEOWN C K , RODRIGUEZ M , et al . Transcriptomic analysis of Clostridium thermocellum ATCC 27405 cellulose fermentation [J ] . BMC Microbiology , 2011 , 11 : 134 .

RYDZAK T , MCQUEEN P D , KROKHIN O V , et al . Proteomic analysis of Clostridium thermocellum core metabolism: relative protein expression profiles and growth phase-dependent changes in protein expression [J ] . BMC Microbiology , 2012 , 12 : 214 .

YANG S H , GIANNONE R J , DICE L , et al . Clostridium thermocellum ATCC 27405 transcriptomic, metabolomic and proteomic profiles after ethanol stress [J ] . BMC Genomics , 2012 , 13 : 336 .

WILSON C M , YANG S H , RODRIGUEZ M , et al . Clostridium thermocellum transcriptomic profiles after exposure to furfural or heat stress [J ] . Biotechnology for Biofuels , 2013 , 6 : 131 .

SANDER K , WILSON C M , RODRIGUEZ M , et al . Clostridium thermocellum DSM 1313 transcriptional responses to redox perturbation [J ] . Biotechnology for Biofuels , 2015 , 8 : 211 .

TIAN L , PEROT S J , STEVENSON D , et al . Metabolome analysis reveals a role for glyceraldehyde 3-phosphate dehydrogenase in the inhibition of C. thermocellum by ethanol [J ] . Biotechnology for Biofuels , 2017 , 10 : 276 .

LI H Y , PAN Y L , CHANG S , et al . Transcriptomic analysis of Clostridium thermocellum in cellulolytic consortium after artificial reconstruction to enhance ethanol production [J ] . BioResources , 2015 , 10 ( 4 ): 7105 - 7122 .

LINVILLE J L , RODRIGUEZ M JR , LAND M , et al . Industrial robustness: understanding the mechanism of tolerance for the Populus hydrolysate-tolerant mutant strain of Clostridium thermocellum [J ] . PLoS One , 2013 , 8 ( 10 ): e78829 .

LINVILLE J L , RODRIGUEZ M , BROWN S D , et al . Transcriptomic analysis of Clostridium thermocellum Populus hydrolysate-tolerant mutant strain shows increase d cellular efficiency in response to Populus hydrolysate compared to the wild type strain [J ] . BMC Microbiology , 2014 , 14 : 215 .

THOMPSON R A , LAYTON D S , GUSS A M , et al . Elucidating central metabolic redox obstacles hindering ethanol production in Clostridium thermocellum [J ] . Metabolic Engineering , 2015 , 32 : 207 - 219 .

THOMPSON R A , DAHAL S , GARCIA S , et al . Exploring complex cellular phenotypes and model-guided strain design with a novel genome-scale metabolic model of Clostridium thermocellum DSM 1313 implementing an adjustable cellulosome [J ] . Biotechnology for Biofuels , 2016 , 9 : 194 .

GARCIA S , THOMPSON R A , GIANNONE R J , et al . Development of a genome-scale metabolic model of Clostridium thermocellum and its applications for integration of multi-omics datasets and computational strain design [J ] . Frontiers in Bioengineering and Biotechnology , 2020 , 8 : 772 .

MOHR G , HONG W , ZHANG J E , et al . A targetron system for gene targeting in thermophiles and its application in Clostridium thermocellum [J ] . PLoS One , 2013 , 8 ( 7 ): e69032 .

WALKER J E , LANAHAN A A , ZHENG T Y , et al . Development of both type I-B and type Ⅱ CRISPR/Cas genome editing systems in the cellulolytic bacterium Clostridium thermocellum [J ] . Metabolic Engineering Communications , 2020 , 10 : e00116 .

GANGULY J , MARTIN-PASCUAL M , VAN KRANENBURG R . CRISPR interference (CRISPRi) as transcriptional repression tool for Hungateiclostridium thermocellum DSM 1313 [J ] . Microbial Biotechnology , 2020 , 13 ( 2 ): 339 - 349 .

GUSS A M , OLSON D G , CAIAZZA N C , et al . Dcm methylation is detrimental to plasmid transformation in Clostridium thermocellum [J ] . Biotechnology for Biofuels , 2012 , 5 ( 1 ): 30 .

ZHANG J , LIU S Y , LI R M , et al . Efficient whole-cell-catalyzing cellulose saccharification using engineered Clostridium thermocellum [J ] . Biotechnology for Biofuels , 2017 , 10 : 124 .

ARGYROS D A , TRIPATHI S A , BARRETT T F , et al . High ethanol titers from cellulose by using metabolically engineered thermophilic, anaerobic microbes [J ] . Applied and Environmental Microbiology , 2011 , 77 ( 23 ): 8288 - 8294 .

KWON S W , PAARI K A , MALAVIYA A , et al . Synthetic biology tools for genome and transcriptome engineering of solventogenic Clostridium [J ] . Frontiers in Bioengineering and Biotechnology , 2020 , 8 : 282 .

KUEHNE S A , MINTON N P . ClosTron-mediated engineering of Clostridium [J ] . Bioengineered , 2012 , 3 ( 4 ): 247 - 254 .

HEAP J T , PENNINGTON O J , CARTMAN S T , et al . The ClosTron: a universal gene knock-out system for the genus Clostridium [J ] . Journal of Microbiological Methods , 2007 , 70 ( 3 ): 452 - 464 .

PARK S K , MOHR G , YAO J , et al . Group Ⅱ intron-like reverse transcriptases function in double-strand break repair [J ] . Cell , 2022 , 185 ( 20 ): 3671 - 3688.e23 .

CUI G Z , HONG W , ZHANG J , et al . Targeted gene engineering in Clostridium cellulolyticum H10 without methylation [J ] . Journal of Microbiological Methods , 2012 , 89 ( 3 ): 201 - 208 .

ENYEART P J , CHIRIELEISON S M , DAO M N , et al . Generalized bacterial genome editing using mobile group Ⅱ introns and Cre-lox [J ] . Molecular Systems Biology , 2013 , 9 : 685 .

ADLI M . The CRISPR tool kit for genome editing and beyond [J ] . Nature Communications , 2018 , 9 : 1911 .

OLSON D G , MCBRIDE J E , JOE SHAW A , et al . Recent progress in consolidated bioprocessing [J ] . Current Opinion in Biotechnology , 2012 , 23 ( 3 ): 396 - 405 .

MCALLISTER K N , SORG J A . CRISPR genome editing systems in the genus Clostridium : a timely advancement [J ] . Journal of Bacteriology , 2019 , 201 ( 16 ): e00219 .

PYNE M E , BRUDER M R , MOO-YOUNG M , et al . Harnessing heterologous and endogenous CRISPR-Cas machineries for efficient markerless genome editing in Clostridium [J ] . Scientific Reports , 2016 , 6 : 25666 .

RICHTER H , ZOEPHEL J , SCHERMULY J , et al . Characterization of CRISPR RNA processing in Clostridium thermocellum and Methanococcus maripaludis [J ] . Nucleic Acids Research , 2012 , 40 ( 19 ): 9887 - 9896 .

RICHTER H , ROMPF J , WIEGEL J , et al . Fragmentation of the CRISPR-Cas Type I-B signature protein Cas8b [J ] . Biochimica et Biophysica Acta (BBA)-General Subjects , 2017 , 1861 ( 11 ): 2993 - 3000 .

ZHAO H , SUN Y J , PETERS J M , et al . Depletion of undecaprenyl pyrophosphate phosphatases disrupts cell envelope biogenesis in Bacillus subtilis [J ] . Journal of Bacteriology , 2016 , 198 : 2925 - 2935 .

LIU X , GALLAY C , KJOS M , et al . High-throughput CRISPRi phenotyping identifies new essential genes in Streptococcus pneumoniae [J ] . Molecular Systems Biology , 2017 , 13 ( 5 ): 931 .

WANG M , HAN J , DUNN J B , et al . Well-to-wheels energy use and greenhouse gas emissions of ethanol from corn, sugarcane and cellulosic biomass for US use [J ] . Environmental Research Letters , 2012 , 7 ( 4 ): 045905 .

LI J J , ZHANG Y L , YANG Y L , et al . Life cycle assessment and techno-economic analysis of ethanol production via coal and its competitors: a comparative study [J ] . Applied Energy , 2022 , 312 : 118791 .

KANNUCHAMY S , MUKUND N , SALEENA L M . Genetic engineering of Clostridium thermocellum DSM 1313 for enhanced ethanol production [J ] . BMC Biotechnology , 2016 , 16 ( Suppl 1 ): 34 .

DIEN B S , COTTA M A , JEFFRIES T W . Bacteria engineered for fuel ethanol production: current status [J ] . Applied Microbiology and Biotechnology , 2003 , 63 ( 3 ): 258 - 266 .

RYDZAK T , LYND L R , GUSS A M . Elimination of formate production in Clostridium thermocellum [J ] . Journal of Industrial Microbiology & Biotechnology , 2015 , 42 ( 9 ): 1263 - 1272 .

RYDZAK T , GARCIA D , STEVENSON D M , et al . Deletion of Type Ⅰ glutamine synthetase deregulates nitrogen metabolism and increases ethanol production in Clostridium thermocellum [J ] . Metabolic Engineering , 2017 , 41 : 182 - 191 .

BISWAS R , ZHENG T Y , OLSON D G , et al . Elimination of hydrogenase active site assembly blocks H 2 production and increases ethanol yield in Clostridium thermocellum [J ] . Biotechnology for Biofuels , 2015 , 8 : 20 .

LI H F , KNUTSON B L , NOKES S E , et al . Metabolic control of Clostridium thermocellum via inhibition of hydrogenase activity and the glucose transport rate [J ] . Applied Microbiology and Biotechnology , 2012 , 93 ( 4 ): 1777 - 1784 .

LO J , OLSON D G , MURPHY S J L , et al . Engineering electron metabolism to increase ethanol production in Clostridium thermocellum [J ] . Metabolic Engineering , 2017 , 39 : 71 - 79 .

ZHENG T Y , OLSON D G , TIAN L , et al . Cofactor specificity of the bifunctional alcohol and aldehyde dehydrogenase (AdhE) in wild-type and mutant Clostridium thermocellum and Thermoanaerobacterium saccharolyticum [J ] . Journal of Bacteriology , 2015 , 197 ( 15 ): 2610 - 2619 .

TIAN L , PAPANEK B , OLSON D G , et al . Simultaneous achievement of high ethanol yield and titer in Clostridium thermocellum [J ] . Biotechnology for Biofuels , 2016 , 9 : 116 .

HOLWERDA E K , OLSON D G , RUPPERTSBERGER N M , et al . Metabolic and evolutionary responses of Clostridium thermocellum to genetic interventions aimed at improving ethanol production [J ] . Biotechnology for Biofuels , 2020 , 13 : 40 .

WILLIAMS T I , COMBS J C , LYNN B C , et al . Proteomic profile changes in membranes of ethanol-tolerant Clostridium thermocellum [J ] . Applied Microbiology and Biotechnology , 2007 , 74 ( 2 ): 422 - 432 .

KIM S K , WESTPHELING J . Engineering a spermidine biosynthetic pathway in Clostridium thermocellum results in increased resistance to furans and increased ethanol production [J ] . Metabolic Engineering , 2018 , 49 : 267 - 274 .

HERRING C D , KENEALY W R , JOE SHAW A , et al . Strain and bioprocess improvement of a thermophilic anaerobe for the production of ethanol from wood [J ] . Biotechnology for Biofuels , 2016 , 9 : 125 .

BERI D , HERRING C D , BLAHOVA S , et al . Coculture with hemicellulose-fermenting microbes reverses inhibition of corn fiber solubilization by Clostridium thermocellum at elevated solids loadings [J ] . Biotechnology for Biofuels , 2021 , 14 ( 1 ): 24 .

CHEN S T , XU Z X , DING B N , et al . Big data mining, rational modification, and ancestral sequence reconstruction inferred multiple xylose isomerases for biorefinery [J ] . Science Advances , 2023 , 9 ( 5 ): eadd8835 .

CHEN X W , KUHN E , JENNINGS E W , et al . DMR (deacetylation and mechanical refining) processing of corn stover achieves high monomeric sugar concentrations (230 g·L -1 ) during enzymatic hydrolysis and high ethanol concentrations ( > 10% v/v) during fermentation without hydrolysate purification or concentration [J ] . Energy & Environmental Science , 2016 , 9 ( 4 ): 1237 - 1245 .

GHOSH I N , LANDICK R . OptSSeq: high-throughput sequencing readout of growth enrichment defines optimal gene expression elements for homoethanologenesis [J ] . ACS Synthetic Biology , 2016 , 5 ( 12 ): 1519 - 1534 .

KHANA D B , CALLAGHAN M M , AMADOR-NOGUEZ D . Novel computational and experimental approaches for investigating the thermodynamics of metabolic networks [J ] . Current Opinion in Microbiology , 2022 , 66 : 21 - 31 .

BHANDIWAD A , SHAW A J , GUSS A , et al . Metabolic engineering of Thermoanaerobacterium saccharolyticum for n -butanol production [J ] . Metabolic Engineering , 2014 , 21 : 17 - 25 .

KELLER M W , LIPSCOMB G L , LODER A J , et al . A hybrid synthetic pathway for butanol production by a hyperthermophilic microbe [J ] . Metabolic Engineering , 2015 , 27 : 101 - 106 .

TIAN L , CONWAY P M , CERVENKA N D , et al . Metabolic engineering of Clostridium thermocellum for n -butanol production from cellulose [J ] . Biotechnology for Biofuels , 2019 , 12 : 186 .

TIAN L , CERVENKA N D , LOW A M , et al . A mutation in the AdhE alcohol dehydrogenase of Clostridium thermocellum increases tolerance to several primary alcohols, including isobutanol, n-butanol and ethanol [J ] . Scientific Reports , 2019 , 9 : 1736 .

PINTO T , FLORES-ALSINA X , GERNAEY K V , et al . Alone or together? A review on pure and mixed microbial cultures for butanol production [J ] . Renewable and Sustainable Energy Reviews , 2021 , 147 : 111244 .

KIYOSHI K , FURUKAWA M , SEYAMA T , et al . Butanol production from alkali-pretreated rice straw by co-culture of Clostridium thermocellum and Clostridium saccharoperbutylacetonicum [J ] . Bioresource Technology , 2015 , 186 : 325 - 328 .

WEN Z Q , WU M B , LIN Y J , et al . A novel strategy for sequential co-culture of Clostridium thermocellum and Clostridium beijerinckii to produce solvents from alkali extracted corn cobs [J ] . Process Biochemistry , 2014 , 49 ( 11 ): 1941 - 1949 .

BEGUM S , DAHMAN Y . Enhanced biobutanol production using novel clostridial fusants in simultaneous saccharification and fermentation of green renewable agriculture residues [J ] . Biofuels, Bioproducts and Biorefining , 2015 , 9 ( 5 ): 529 - 544 .

LAKSHMI N M , BINOD P , SINDHU R , et al . Microbial engineering for the production of isobutanol: current status and future directions [J ] . Bioengineered , 2021 , 12 ( 2 ): 12308 - 12321 .

HON S , HOLWERDA E K , WORTHEN R S , et al . Expressing the Thermoanaerobacterium saccharolyticum pforA in engineered Clostridium thermocellum improves ethanol production [J ] . Biotechnology for Biofuels , 2018 , 11 : 242 .

LIN P P , RABE K S , TAKASUMI J L , et al . Isobutanol production at elevated temperatures in thermophilic Geobacillus thermoglucosidasius [J ] . Metabolic Engineering , 2014 , 24 : 1 - 8 .

DONG H J , ZHAO C H , ZHANG T R , et al . A systematically chromosomally engineered Escherichia coli efficiently produces butanol [J ] . Metabolic Engineering , 2017 , 44 : 284 - 292 .

ZHAO C H , SINUMVAYO J P , ZHANG Y P , et al . Design and development of a "Y-shaped" microbial consortium capable of simultaneously utilizing biomass sugars for efficient production of butanol [J ] . Metabolic Engineering , 2019 , 55 : 111 - 119 .

ZHAN Y Y , XU Y , LU X C , et al . Metabolic engineering of Bacillus licheniformis for sustainable production of isobutanol [J ] . ACS Sustainable Chemistry & Engineering , 2021 , 9 ( 51 ): 17254 - 17265 .

BAEZ A , CHO K M , LIAO J C . High-flux isobutanol production using engineered Escherichia coli : a bioreactor study with in situ product removal [J ] . Applied Microbiology and Biotechnology , 2011 , 90 ( 5 ): 1681 - 1690 .

DODDS P E , STAFFELL I , HAWKES A D , et al . Hydrogen and fuel cell technologies for heating: a review [J ] . International Journal of Hydrogen Energy , 2015 , 40 ( 5 ): 2065 - 2083 .

OLADOKUN O , AHMAD A , ABDULLAH T A T , et al . Biohydrogen production from Imperata cylindrica bio-oil using non-stoichiometric and thermodynamic model [J ] . International Journal of Hydrogen Energy , 2017 , 42 ( 14 ): 9011 - 9023 .

LEVIN D B , ISLAM R , CICEK N , et al . Hydrogen production by Clostridium thermocellum 27405 from cellulosic biomass substrates [J ] . International Journal of Hydrogen Energy , 2006 , 31 ( 11 ): 1496 - 1503 .

TIAN Q Q , LIANG L , ZHU M J . Enhanced biohydrogen production from sugarcane bagasse by Clostridium thermocellum supplemented with CaCO 3 [J ] . Bioresource Technology , 2015 , 197 : 422 - 428 .

KIM D H , SHIN H S , KIM S H . Enhanced H 2 fermentation of organic waste by CO 2 sparging [J ] . International Journal of Hydrogen Energy , 2012 , 37 ( 20 ): 15563 - 15568 .

KIM C , WOLF I , DOU C , et al . Coupling gas purging with inorganic carbon supply to enhance biohydrogen production with Clostridium thermocellum [J ] . Chemical Engineering Journal , 2023 , 456 : 141028 .

LEE H S , VERMAAS W F J , RITTMANN B E . Biological hydrogen production: prospects and challenges [J ] . Trends in Biotechnology , 2010 , 28 ( 5 ): 262 - 271 .

LI Q , LIU C Z . Co-culture of Clostridium thermocellum and Clostridium thermosaccharolyticum for enhancing hydrogen production via thermophilic fermentation of cornstalk waste [J ] . International Journal of Hydrogen Energy , 2012 , 37 ( 14 ): 10648 - 10654 .

AN Q , BU J , CHENG J R , et al . Biological saccharification by Clostridium thermocellum and two-stage hydrogen and methane production from hydrogen peroxide-acetic acid pretreated sugarcane bagasse [J ] . International Journal of Hydrogen Energy , 2020 , 45 ( 55 ): 30211 - 30221 .

ALVES DE OLIVEIRA R , KOMESU A , VAZ ROSSELL C E , et al . Challenges and opportunities in lactic acid bioprocess design—from economic to production aspects [J ] . Biochemical Engineering Journal , 2018 , 133 : 219 - 239 .

TARRARAN L , MAZZOLI R . Alternative strategies for lignocellulose fermentation through lactic acid bacteria: the state of the art and perspectives [J ] . FEMS Microbiology Letters , 2018 , 365 ( 15 ): fny126 .

LO J , ZHENG T Y , HON S , et al . The bifunctional alcohol and aldehyde dehydrogenase gene, adhE , is necessary for ethanol production in Clostridium thermocellum and Thermoanaerobacterium saccharolyticum [J ] . Journal of Bacteriology , 2015 , 197 ( 8 ): 1386 - 1393 .

MAZZOLI R , OLSON D G , LYND L R . Construction of lactic acid overproducing Clostridium thermocellum through enhancement of lactate dehydrogenase expression [J ] . Enzyme and Microbial Technology , 2020 , 141 : 109645 .

SUBRAMANIAN M R , TALLURI S , CHRISTOPHER L P . Production of lactic acid using a new homofermentative Enterococcus faecalis isolate [J ] . Microbial Biotechnology , 2015 , 8 ( 2 ): 221 - 229 .

WEN Z Q , LEDESMA-AMARO R , LU M R , et al . Combined evolutionary engineering and genetic manipulation improve low pH toleranc e and butanol production in a synthetic microbial Clostridium community [J ] . Biotechnology and Bioengineering , 2020 , 117 ( 7 ): 2008 - 2022 .

MAZZOLI R , OLSON D G , CONCU A M , et al . In vivo evolution of lactic acid hyper-tolerant Clostridium thermocellum [J ] . New Biotechnology , 2022 , 67 : 12 - 22 .

KRUIS A J , BOHNENKAMP A C , PATINIOS C , et al . Microbial production of short and medium chain esters: enzymes, pathways, and applications [J ] . Biotechnology Advances , 2019 , 37 ( 7 ): 107407 .

RODRIGUEZ G M , TASHIRO Y , ATSUMI S . Expanding ester biosynthesis in Escherichia coli [J ] . Nature Chemical Biology , 2014 , 10 ( 4 ): 259 - 265 .

SEO H , LEE J W , GARCIA S , et al . Single mutation at a highly conserved region of chloramphenicol acetyltransferase enables isobutyl acetate production directly from cellulose by Clostridium thermocellum at elevated temperatures [J ] . Biotechnology for Biofuels , 2019 , 12 : 245 .

SEO H , NICELY P N , TRINH C T . Endogenous carbohydrate esterases of Clostridium thermocellum are identified and disrupted for enhanced isobutyl acetate production from cellulose [J ] . Biotechnology and Bioengineering , 2020 , 117 ( 7 ): 2223 - 2236 .

SEO H , LEE J W , GIANNONE R J , et al . Engineering promiscuity of chloramphenicol acetyltransferase for microbial designer ester biosynthesis [J ] . Metabolic Engineering , 2021 , 66 : 179 - 190 .

SEO H , SINGH P , WYMAN C E , et al . Rewiring metabolism of Clostridium thermocellum for consolidated bioprocessing of lignocellulosic biomass poplar to produce short-chain esters [J ] . Bioresource Technology , 2023 , 384 : 129263 .

LIU S Y , LIU Y J , FENG Y G , et al . Construction of consolidated bio-saccharification biocatalyst and process optimization for highly efficient lignocellulose solubilization [J ] . Biotechnology for Biofuels , 2019 , 12 : 35 .

QI K , CHEN C , YAN F , et al . Coordinated β-glucosidase activity with the cellulosome is effective for enhanced lignocellulose saccharification [J ] . Bioresource Technology , 2021 , 337 : 125441 .

OLGUIN-MACIEL E , SINGH A , CHABLE-VILLACIS R , et al . Consolidated bioprocessing, an innovative strategy towards sustainability for biofuels production from crop residues: an overview [J ] . Agronomy , 2020 , 10 ( 11 ): 1834 .

PATEL A , SHAH A R . Integrated lignocellulosic biorefinery: gateway for production of second generation ethanol and value added products [J ] . Journal of Bioresources and Bioproducts , 2021 , 6 ( 2 ): 108 - 128 .

ARORA R , SINGH P , SARANGI P K , et al . A critical assessment on scalable technologies using high solids loadings in lignocellulose biorefinery: challenges and solutions [J/OL ] . Critical Reviews in Biotechnology , 2023 [ 2023-06-01 ] . https://www.tandfonline.com/doi/abs/10.1080/07388551.2022.2151409 https://www.tandfonline.com/doi/abs/10.1080/07388551.2022.2151409 .

REIS C E R , LIBARDI N , BENTO H B S , et al . Process strategies to reduce cellulase enzyme loading for renewable sugar production in biorefineries [J ] . Chemical Engineering Journal , 2023 , 451 : 138690 .

ICHIKAWA S , ICHIHARA M , ITO T , et al . Glucose production from cellulose through biological simultaneous enzyme production and saccharification using recombinant bacteria expressing the β-glucosidase gene [J ] . Journal of Bioscience and Bioengineering , 2019 , 127 ( 3 ): 340 - 344 .

DASH S , OLSON D G , CHAN S H J , et al . Thermodynamic analysis of the pathway for ethanol production from cellobiose in Clostridium thermocellum [J ] . Metabolic Engineering , 2019 , 55 : 161 - 169 .

HON S , OLSON D G , HOLWERDA E K , et al . The ethanol pathway from Thermoanaerobacterium saccharolyticum improves ethanol production in Clostridium thermocellum [J ] . Metabolic Engineering , 2017 , 42 : 175 - 184 .

SANDER K , ASANO K G , BHANDARI D , et al . Targeted redox and energy cofactor metabolomics in Clostridium thermocellum and Thermoanaerobacterium saccharolyticum [J ] . Biotechnology for Biofuels , 2017 , 10 : 270 .

CUI J X , OLSON D G , LYND L R . Characterization of the Clostridium thermocellum AdhE, NfnAB, ferredoxin and Pfor proteins for their ability to support high titer ethanol production in Thermoanaerobacterium saccharolyticum [J ] . Metabolic Engineering , 2019 , 51 : 32 - 42 .

CHIRANIA P , HOLWERDA E K , GIANNONE R J , et al . Metaproteomics reveals enzymatic strategies deployed by anaerobic microbiomes to maintain lignocellulose deconstruction at high solids [J ] . Nature Communications , 2022 , 13 : 3870 .

BALCH M L , HOLWERDA E K , DAVIS M F , et al . Lignocellulose fermentation and residual solids characterization for senescent switchgrass fermentation by Clostridium thermocellum in the presence and absence of continuous in situ ball-milling [J ] . Energy & Environmental Science , 2017 , 10 ( 5 ): 1252 - 1261 .

YAN F , WEI R , CUI Q , et al . Thermophilic whole-cell degradation of polyethylene terephthalate using engineered Clostridium thermocellum [J ] . Microbial Biotechnology , 2021 , 14 ( 2 ): 374 - 385 .

XIAO Y , DONG S , LIU Y J , et al . Key roles of β-glucosidase BglA for the catabolism of both laminaribiose and cellobiose in the lignocellulolytic bacterium Clostridium thermocellum [J ] . International Journal of Biological Macromolecules , 2023 , 250 : 126226 .

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Progress in synthetic biology research of Clostridium thermocellum for biomass energy applications

DOI：10.12211/2096-8280.2023-046

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