Progress on bio-fixation and utilization of CO<sub>2</sub> in acetogens driven by chemical energy

WEN Zhiqiong; LI Yuzhen; ZHANG Jin′gang; Wang Feifei; MA Xiaoqing; LI Fuli

doi:10.12211/2096-8280.2023-045

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Progress on bio-fixation and utilization of CO₂ in acetogens driven by chemical energy

Invited Review | 更新时间：2025-03-20

- Progress on bio-fixation and utilization of CO₂ in acetogens driven by chemical energy
- Synthetic Biology Journal Vol. 4, Issue 6, Pages: 1178-1190(2023)
- 作者机构：
  
  1.中国科学院青岛生物能源与过程研究所，中国科学院生物燃料重点实验室，青岛市碳一炼制工程研究中心，山东青岛266101
  2.山东荣信集团有限公司，山东济宁 273517
- 作者简介：
- 基金信息：
- DOI：10.12211/2096-8280.2023-045
  CLC： Q81
- Received：30 June 2023，
  
  Revised：2023-09-14，
  
  Published：31 December 2023
- 稿件说明：
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文志琼，李煜真，张金刚，王菲菲，马小清，李福利. 化能驱动的产乙酸菌转化利用CO₂研究进展［J］. 合成生物学， 2023， 4（6）： 1178-1190

WEN Zhiqiong， LI Yuzhen， ZHANG Jin′gang， Wang Feifei， MA Xiaoqing， LI Fuli. Progress on bio-fixation and utilization of CO₂ in acetogens driven by chemical energy［J］. Synthetic Biology Journal， 2023， 4（6）： 1178-1190
文志琼，李煜真，张金刚，王菲菲，马小清，李福利. 化能驱动的产乙酸菌转化利用CO₂研究进展［J］. 合成生物学， 2023， 4（6）： 1178-1190 DOI： 10.12211/2096-8280.2023-045.

WEN Zhiqiong， LI Yuzhen， ZHANG Jin′gang， Wang Feifei， MA Xiaoqing， LI Fuli. Progress on bio-fixation and utilization of CO₂ in acetogens driven by chemical energy［J］. Synthetic Biology Journal， 2023， 4（6）： 1178-1190 DOI： 10.12211/2096-8280.2023-045.

摘要

利用微生物发酵一碳气体生产燃料和高值化学品，是实现碳资源回收利用和绿色生物制造的重要途径之一。产乙酸菌可利用CO或H

为能量来源，通过伍德-永达尔（Wood-Ljungdahl）途径固定CO

，维持自身代谢，并生产乙酸、乙醇等高附加值产品。相较于化学催化而言，该生物催化过程对原料气体比例要求不高、反应条件温和、产物选择性高。在气体发酵中，CO和H

均可作为菌株生长和代谢的能量来源，二者具有不同的能量代谢模式。同时，不同的产乙酸菌代谢产物也不相同。发酵过程中气体溶解度低限制了能量供给，导致微生物生长速率慢、原料利用率低以及目标产物产量低等问题。提高气体利用效率和扩大产乙酸菌的产物谱，是面向产业化应用的必然要求。本文简述了产乙酸菌的伍德-永达尔途径、产乙酸途径和产乙醇途径等天然代谢途径，从分子改造的角度总结了产乙酸菌利用一碳气体发酵生产高附加值产品如乙醇、2，3-丁二醇、丙酮、异丙醇等的相关研究进展，并从提高能量供给和产物选择性的角度进行了总结。最后，本文以能量代谢为切入点对产乙酸菌转化一碳气体进行了展望，以期为未来的生物制造提供参考。

Abstract

To promote the development of carbon utilization and biomanufacturing industry

one-carbon substrates are expected to be the next generation feedstocks and attracted much attention because of their abundance and availability for value-added products. Acetog

ens can naturally use syngas (CO

and H

) through the classical Wood-Ljungdahl pathway to support their growth and produce value-added chemicals such as acetic acid and ethanol. Compared to chemical catalysis

this biorefinery process has the advantages of low requirements for feedstocks

mild reaction conditions and high product selectivity. In some acetogens

both CO and H

can act as energy sources to provide the reducing equivalents required for growth and metabolism during gas fermentation. In particular

acetogens utilizing CO and H

have distinct patterns of energy metabolism. Compared to H

CO is a more thermodynamically favorable energy source for gas fermentation. In general

different acetogens during autotrophic growth with syngas as carbon source and energy source can produce different products. However

the low solubility of gases in liquid medium

as well as the poor mass transfer and diffusion

limits the energy supply during the fermentation

resulting in slow growth rate of microorganisms

low raw material utilization

and low target product yield. Therefore

improving syngas utilization efficiency and enlarging products spectrum of acetogens are both of great importance to meet the demands of industrialization. In recent years

with the development of synthetic biology and molecular genetic tools

modification of acetogen strains has been well designed. In this review

we briefly described the natural metabolic pathways such as ethanol and acetone metabolism pathways

and summarized the progress of acetogens in autotrophic fermentation using syngas as energy sources to produce value-added products such as ethanol

3-butanediol

acetone and isoprepanol. For engineering the acetogens

we summarized the pathways to produce natural and non-natural chemicals. Finally

the energy capture and utilization of acetogens was prospected

with the aim to facilitate the future advancement of biorefineries.

关键词

Keywords

references

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⁰

Progress on bio-fixation and utilization of CO2 in acetogens driven by chemical energy

DOI：10.12211/2096-8280.2023-045

摘要

Abstract

关键词

Keywords

references

Progress on bio-fixation and utilization of CO₂ in acetogens driven by chemical energy