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1.中国科学院深圳先进技术研究院,深圳合成生物学创新研究院,合成生物化学研究中心,广东 深圳 518055
2.中国科学院深圳先进技术研究院,深圳合成生物学创新研究院,中国科学院定量工程生物学重点实验室,广东 深圳 518055
Received:13 June 2023,
Revised:2024-01-30,
Published:31 January 2025
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郭姝媛, 张倩楠, 姑丽克孜·买买提热夏提, 杨一群, 于涛. 液体生物燃料合成与炼制的研究进展[J]. 合成生物学, 2025, 6(1): 18-44
GUO Shuyuan, ZHANG Qiannan, Gulikezi·MAIMAITIREXIATI , YANG Yiqun, YU Tao. Advances in microbial production of liquid biofuels[J]. Synthetic Biology Journal, 2025, 6(1): 18-44
郭姝媛, 张倩楠, 姑丽克孜·买买提热夏提, 杨一群, 于涛. 液体生物燃料合成与炼制的研究进展[J]. 合成生物学, 2025, 6(1): 18-44 DOI: 10.12211/2096-8280.2023-040.
GUO Shuyuan, ZHANG Qiannan, Gulikezi·MAIMAITIREXIATI , YANG Yiqun, YU Tao. Advances in microbial production of liquid biofuels[J]. Synthetic Biology Journal, 2025, 6(1): 18-44 DOI: 10.12211/2096-8280.2023-040.
人类社会发展对化石燃料的依赖导致了资源枯竭的加剧及显著的气候变化,迫切需要开发能够代替化石燃料的新型生物燃料。虽然已有生物乙醇和生物柴油等生物能源,但其生产规模和成本仍然是大规模应用的主要问题。近年来,随着可再生能源技术的发展,结合代谢工程及新兴的合成生物学,开发基于CO
2
合成的新兴生物燃料,逐渐成为未来绿色能源的重要研究方向。本文综述了生物燃料的种类及四代生物燃料的发展情况,并着重介绍了第三代和第四代生物燃料丰富的底物原材料、多能源偶联合成生物燃料的研究现状、合成生物学在其中的应用及现阶段的研究进展。最后概括了合成生物燃料所面临的困境,主要包括原料的供应及成本,新型液体生物燃料产量低和产品种类少等问题,并提出相应的解决办法,以二氧化碳作为主要原材料,结合自养型微生物及甲基营养型微生物等细胞工厂,通过优选固碳途径、转化二氧化碳为甲醇等低碳底物及多能源耦合等方式实现多种生物燃料的合成,以期扩大生物燃料的产能及应用范围,进一步推动新型生物燃料的产业化进程。
With the socioeconomic development
the dependence of human beings on fossil fuels has led to their shortage and climate change. This has created an urgent need for alternatives that are renewable and environmentally friendly
and biofuels are one of them. Nowadays
widely recognized b
iofuels like fuel ethanol and biodiesel face challenges in terms of their production capacity due to limitation on raw materials such as grains and edible oils and high cost as well. Hence
the integration of metabolic engineering and synthetic biology has opened avenues for utilizing diverse substrates from other renewable sources
such as solar energy
light energy
electric energy
and waste biomass. Microbial cell factories
including microalgae
bacteria
and yeast
play a crucial role in synthesizing biofuels. The review comments on the evolution of the four generations of biofuels
encompassing fuel ethanol
biodiesel
bio-gasoline
jet and aviation fuels. We also discuss how microorganisms can be explored for producing the third- and fourth-generation biofuels from a variety of unconventional substrates such as carbon dioxide
methanol
and methane
multi-energy coupling to synthesize biofuels from lignocellulose by bacterial or yeast
CO
2
conversion by microalgae or electrochemical-biological systems
the conversion of methanol and methane by methyltrophic microbes
and the application of synthetic biology. Furthermore
we overview biosynthetic pathways and engineering strategies for optimizing biofuels production. These strategies can convert raw materials to various fuel products
including fatty acids and esters
advanced alcohols and esters
isoprenoids
and polyketides. Finally
we highlight some challenges in biofuels production
including raw material supply and cost issue
low production yield
and limited product variety. Meanwhile
to address these challenges
we propose corresponding solutions. For example
by optimizing carbon fixation pathways
and converting carbon dioxide into low-carbon substrates like methanol
autotrophic microorganisms
methylotrophic microorganisms
and other cell factories can utilize carbon dioxide as the major raw material to synthesize various biofuels
which can benefit the application of biofuels and further promote their industrial production.
2
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