Progress and prospect of ultrasonic liquid transfer and low-volume liquid transfer technology

ZHANG zhiqiang; ZHANG Yang; QIU Weibao; ZHENG Hairong

doi:10.12211/2096-8280.2023-036

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Progress and prospect of ultrasonic liquid transfer and low-volume liquid transfer technology

Invited Review | 更新时间：2025-03-20

- Progress and prospect of ultrasonic liquid transfer and low-volume liquid transfer technology
- Synthetic Biology Journal Vol. 4, Issue 5, Pages: 916-931(2023)
- 作者机构：
  
  1.中国科学院深圳先进技术研究院，保罗·C·劳特伯生物医学成像研究中心，广东深圳 518055
  2.南华大学电气工程学院，湖南衡阳 421001
- 作者简介：
- 基金信息：
- DOI：10.12211/2096-8280.2023-036
  CLC： Q819
- Received：10 May 2023，
  
  Revised：2023-07-05，
  
  Published：31 October 2023
- 稿件说明：
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张志强，张扬，邱维宝，郑海荣. 超声移液及微量移液技术进展和展望［J］. 合成生物学， 2023， 4（5）： 916-931

ZHANG zhiqiang， ZHANG Yang， QIU Weibao， ZHENG Hairong. Progress and prospect of ultrasonic liquid transfer and low-volume liquid transfer technology［J］. Synthetic Biology Journal， 2023， 4（5）： 916-931
张志强，张扬，邱维宝，郑海荣. 超声移液及微量移液技术进展和展望［J］. 合成生物学， 2023， 4（5）： 916-931 DOI： 10.12211/2096-8280.2023-036.

ZHANG zhiqiang， ZHANG Yang， QIU Weibao， ZHENG Hairong. Progress and prospect of ultrasonic liquid transfer and low-volume liquid transfer technology［J］. Synthetic Biology Journal， 2023， 4（5）： 916-931 DOI： 10.12211/2096-8280.2023-036.

摘要

近年来，合成生物学、新药研究以及体外诊断等现代生物、医学技术的快速发展对微量移液技术的精准度、通量、成本等各方面提出了越来越高的要求。传统基于活塞原理的移液技术虽然可以做到自动化，通量可以很高，但是移液精度局限于亚微升级。基于电磁阀、压电驱动等移液技术可以大幅度提高移液精度，但是由于结构复杂，通量难以与活塞式移液技术相媲美。基于电场、磁场、激光等新型移液技术的移液精度可以实现纳升级和皮升级，但是这些技术主要基于微流控平台，针对一些特定应用，通用性差。此外，上述移液技术都需要使用吸头、毛细管、喷嘴等移液头，会与液体直接接触，存在移液头容易堵塞、液体残留、样品交叉污染的风险，而且移液头大都是一次性耗材，成本高，污染环境。非接触式超声移液技术是一种声镊技术，利用超声波声场调制的声辐射力实现对液滴的无接触式操控，无需一次性移液头辅助，无需与液体接触，且具有精度高、移液速度快等特点，是较为理想的精密微量移液技术，展现了重要的应用前景。本文对微量移液技术的发展和代表性研究进展进行了系统性介绍，重点介绍了非接触式超声移液技术的发展和研究进展，并分析讨论了微量移液技术值得关注的发展方向，比如高通量及高通用性的非接触式超声移液技术、智能化移液工作站，以及基于微流控平台的微量液体处理技术等。

Abstract

In recent years

the rapid development of modern biological and medical technologies

such as synthetic biology

new drug research

and

in vitro

diagnosis

has put forward increasingly high requirements on the precision

accuracy

throughput and cost of low-volume liquid transfer technology as the number of liquid samples increases but the volume of liquid samples decreases greatly. Although the traditional piston-based pipetting technology can achieve automation and high throughput

the pipetting precision is limited to sub-microliter

and it consumes a large amount of disposable pipette tips. The liquid transfer technologies based on solenoid valve and piezoelectric actuator can improve liquid transfer precision greatly

however

the throughput of these technologies are lower than pipetting technology due to their complex structures. The liquid transfer technologies based on electric field

magnetic field

and light can achieve high transfer precision of nanoliter and picoliter

but these technologies are mainly based on the microfluidic platform for some special applications. In addition

the tips such as the pipette tip

tubing

or nozzle used in the aforementioned liquid transfer technologies are in direct contact with the liquid

leading to the risks of tip blockage

liquid residue and sample cross-contamination. Moreover

the tips are mostly disposable

resulting in high cost and environmental pollution. Non-contact ultrasonic liquid transfer technology

using acoustic radiation force of focused ultrasonic wave to eject droplets from liquid surface

does not need disposable tips

and the ejected d

roplets do not contact with any other media except the liquid containers during the transfer process. The size of ejected droplets can be accurately controlled by adjusting the focus size and acoustic energy of ultrasound beam. The liquid transfer volume can be adjusted over a large range from nanoliter to picoliter with high precision. Due to its characteristics of fully contact-free

high precision and high transfer speed

non-contact ultrasonic liquid transfer technology shows great potential in biological and medical applications. In this paper

we introduce the development and representative progress of low-volume liquid transfer technology

with emphasis on the development and progress of non-contact ultrasonic liquid transfer technology. Finally the future trends of low-volume liquid transfer technology are analyzed and discussed

such as non-contact ultrasonic liquid transfer technology with high throughput and good versatility

intelligent liquid handling workstation

and low-volume liquid handling technology based on microfluidic platform.

关键词

Keywords

references

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