Application of automated high-throughput technology in natural product biosynthesis

HU Zhehui; XU Juan; BIAN Guangkai

doi:10.12211/2096-8280.2023-035

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Application of automated high-throughput technology in natural product biosynthesis

Invited Review | 更新时间：2025-03-20

- Application of automated high-throughput technology in natural product biosynthesis
- Synthetic Biology Journal Vol. 4, Issue 5, Pages: 932-946(2023)
- 作者机构：
  
  1.中国科学院深圳先进技术研究院合成生物学研究所，广东深圳 518055
  2.华中农业大学园艺林学学院，果蔬园艺作物种质创新与利用全国重点实验室，湖北武汉 430071
- 作者简介：
- 基金信息：
- DOI：10.12211/2096-8280.2023-035
  CLC： Q816
- Received：06 May 2023，
  
  Revised：2023-07-24，
  
  Published：31 October 2023
- 稿件说明：
移动端阅览
胡哲辉，徐娟，卞光凯. 自动化高通量技术在天然产物生物合成中的应用［J］. 合成生物学， 2023， 4（5）： 932-946

HU Zhehui， XU Juan， BIAN Guangkai. Application of automated high-throughput technology in natural product biosynthesis［J］. Synthetic Biology Journal， 2023， 4（5）： 932-946
胡哲辉，徐娟，卞光凯. 自动化高通量技术在天然产物生物合成中的应用［J］. 合成生物学， 2023， 4（5）： 932-946 DOI： 10.12211/2096-8280.2023-035.

HU Zhehui， XU Juan， BIAN Guangkai. Application of automated high-throughput technology in natural product biosynthesis［J］. Synthetic Biology Journal， 2023， 4（5）： 932-946 DOI： 10.12211/2096-8280.2023-035.

摘要

天然产物与人们的日常生活息息相关，是药物、食品、营养添加剂、色素、化妆品等产品的重要来源。发掘新的天然产物并实现其高效合成对于提升人们的生活水平至关重要。然而，研究通量低和产物产量低已成为限制该领域快速发展的两大瓶颈。为了克服这些问题，自动化高通量技术的引入在天然产物生物合成领域具有重要意义。该技术可以将高度依赖人力资源的低通量和随机性手工操作的实验过程转变为基于先进设备的自动化、标准化和高效的研究过程。因此，将自动化高通量技术引入天然产物生物合成领域可以有效解决研究瓶颈，加速研发进程。本文综述了自动化高通量技术在天然产物生物合成领域的应用，阐述了其在天然产物挖掘、高效生物合成和快速检测等方面的优势。最后，讨论了该技术现存缺陷，包括仪器设备造价高昂、操作复杂，并且一些装置尚未得到推广。相信在合成生物、信息技术、自动化等多领域的共同协作下，自动化高通量技术具备的独特优势将为新型天然产物功能产品的开发提供可持续来源。

Abstract

Natural products are usually secondary metabolites derived from animals

plants

or microorganisms. They are closely related to people's daily lives and important sources of drugs

food and nutrition additives

pigments

and cosmetics. The exploration of novel natural products and the efficient synthesis of value-added products are key to enriching and improving people's living standards. Researchers have exploited a large number of natural products through strategies such as direct product isolation

homologous activation

and heterologous expression. However

low throughput and low titer have become the two major bottlenecks of this field. With the rapid development of synthetic biology

automated high-throughput technology has been developed to transform the low throughput

stochastic manual experimental process that relies on human resources into an automated

standardized

and efficient research process

which is regarded as a leading technology in natural product biosynthesis. This review compares and highlights the advantages of automated high-throughput technology over the traditional techniques within the context of natural products biosynthesis

provides an overview of the components and principles of automated high-throughput workstations

and summarizes the existing and upcoming automated high-throughput facilities. In addition

this review highlights the applications of automated high-throughput technology

including the batch mining of natural product biosynthetic genes and gene clusters

overproduction

and efficient detection of value-added natural products. These examples effectively illustrate the benefits of this technology in the field of natural product biosynthesis. In conclusion

the use of automated high-throughput technology in synthetic biology has shown promising results

particularly in the high-throughput discovery and biosynthesis of natural products. Finally

we discuss the existing shortcomings of the technology. Despite the cost and operational challenges associated with the automated high-throughput technology

it is expected to significantly advance both basic and applied research in synthetic biology

while also provide a sustainable source for the development of new functional products based on newly discovered natural products.

关键词

Keywords

references

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Applications of the CRISPR/Cas9 editing system in the study of microbial natural products

Recent advances in heterologous production of natural products using Aspergillus oryzae

From chemical synthesis to biosynthesis: trends toward total synthesis of natural products

Related Author

Hu Zhehui

WEI Lingzhen

WANG Jia

SUN Xinxiao

YUAN Qipeng

SHEN Xiaolin

HUI Zhen

TANG Xiaoyu

Related Institution

State Key Laboratory of Chemical Resource Engineering， Beijing University of Chemical Technology

Department of Chemistry， School of Science， The Hong Kong University of Science and Technology， Clearwater Bay Campus

Institute of Chemical Biology， Shenzhen Bay Laboratory

Key Laboratory of Natural Products Synthetic Biology of Ethnic Medicinal Endophytes， State Ethnic Affairs Commission， and Key Laboratory of Chemistry in Ethnic Medicinal Resources， State Ethnic Affairs Commission and Ministry of Education， Yunnan Minzu University

Department of Chemistry， City University of Hong Kong， Kowloon

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