Multifunction microplate reader for automated foundry platform

MA Cui; YANG Fan; ZHANG Juntai; HE Kai

doi:10.12211/2096-8280.2023-023

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Multifunction microplate reader for automated foundry platform

Research Article | 更新时间：2025-03-20

- Multifunction microplate reader for automated foundry platform
- Synthetic Biology Journal Vol. 4, Issue 5, Pages: 1036-1049(2023)
- 作者机构：
  
  1.中国科学院深圳先进技术研究院，广东　深圳　518055
  2.深圳市精密工程重点实验室，广东　深圳　518055
- 作者简介：
- 基金信息：
- DOI：10.12211/2096-8280.2023-023
  CLC： TH773;Q819
- Received：16 March 2023，
  
  Revised：2023-07-11，
  
  Published：31 October 2023
- 稿件说明：
移动端阅览
马翠，杨凡，张君泰，何凯. 面向自动化铸造平台的多功能微孔板检测系统［J］. 合成生物学， 2023， 4（5）： 1036-1049

MA Cui， YANG Fan， ZHANG Juntai， HE Kai. Multifunction microplate reader for automated foundry platform［J］. Synthetic Biology Journal， 2023， 4（5）： 1036-1049
马翠，杨凡，张君泰，何凯. 面向自动化铸造平台的多功能微孔板检测系统［J］. 合成生物学， 2023， 4（5）： 1036-1049 DOI： 10.12211/2096-8280.2023-023.

MA Cui， YANG Fan， ZHANG Juntai， HE Kai. Multifunction microplate reader for automated foundry platform［J］. Synthetic Biology Journal， 2023， 4（5）： 1036-1049 DOI： 10.12211/2096-8280.2023-023.

摘要

生物分析技术正向高通量、高灵敏度、多功能方向发展，微孔板检测仪作为高通量分析的基础仪器，需要满足微量化、自动化、集成化的要求。目前国内单一功能的微孔板检测仪器技术成熟，检测精度已与国际水平相当，但多功能的微孔板检测仪还较少，大多使用进口仪器。进口产品价格昂贵，有些操作指令不公开，不易集成到自主研发的合成生物自动化铸造平台项目。本研究将对高精度吸光度检测和高灵敏度荧光检测等关键技术进行研究，开发具有吸光检测和荧光检测功能的微孔板检测仪，并自主开发控制系统及数据采集分析软件，使其易于接入自动化铸造平台。该微孔板检测仪结构紧凑，吸光检测和荧光检测模块独立，波段可扩展。通过实验测试，所研发的微孔板检测仪的吸光度检测重复性为0.3%，10 s内可完成96孔的吸光检测；荧光检测数据在皮摩尔浓度范围内线性度较好，对荧光素钠溶液的检测灵敏度大约3.9 pmol/L。此外，该系统可通过串口直接连接自动化平台集成控制系统，也可通过TCP方式进行远程通信控制。

Abstract

Bioanalytical technology is being developed towards the direction of high throughput

high sensitivity and multi-functionality. Microplate readers serve as fundamental instruments for high-throughput analysis. They need to meet the requirements of microanalysis

automation and integration. At present

domestic instruments have limitations in terms of functionality and automation. Although the single-function microplate reader has basically reached the level of oversea equivalents

there is still a gap in the multifunctional instruments. Most of the multi-function microplate reader used are imported instruments. They are usually expensive and some operating instructions are not fully disclosed. It is difficult to integrate them into the automated foundry platform. In this study

we will research key technologies of high-precision absorbance detection and high-sensitivity fluorescence detection

and develop a multifunction microplate reader. The control and soft system is also developed independently to access the automated foundry platform. In our system

the absorbance optical system includes light source

filters

fibers and lens array. Multiple channels of transmitted light are detected in parallel to improve the speed of detection. Combined with the one-dimensional movement of microplate

the whole 96 wells are measured. The fluorescence optical system mainly includes LED light source

filter cube (emission filter

excitation filter and dichroic mirror) and photodiode detector. Single channel of excitation light is detected and two-dimension scanning mechanism is realized for the whole microplate measurement. Besides the optical system

high precision data acquisition system is developed

including signal amplifying

conditioning and isolating circuit. This microplate reader is compact with independent absorbance and fluorescence modules

and scalable wavebands. According to experimental results

the reproducibility of absorbance measurement is high with a standard error of 0.3% and the total 96 micro-wells can be detected accurately within 10 seconds. The fluorescence detection has good linearity in the picomole concentration and the limit of detection for sodium fluorescein is about 3.9 pmol/L. For synthetic biology automated foundry platform

this microplate reader can be directly connected to the integration control system through serial port

and can also be remotely controlled by TCP communication.

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references

WALKOWIAK B , KESY A , MICHALEC L . Microplate reader-a convenient tool in studies of blood coagulation [J ] . Thrombosis Research , 1997 , 87 ( 1 ): 95 - 103 .

MARESOVÁ L , SYCHROVÁ H . Applications of a microplate reader in yeast physiology research [J ] . BioTechniques , 2007 , 43 ( 5 ): 667 - 672 .

WAN Z J , ZHONG L J , PAN Y X , et al . Portable microplate analyzer with a thermostatic chamber based on a smartphone for on-site rapid detection [J ] . Analytical Sciences , 2017 , 33 ( 11 ): 1291 - 1296 .

张国斌 . 酶标仪检测技术应用分析 [J ] . 中国医疗器械信息 , 2021 , 27 ( 21 ): 158 - 160 .

ZHANG G B . Analysis of application of enzyme-plate reader detection technology [J ] . China Medical Device Information , 2021 , 27 ( 21 ): 158 - 160 .

Agilent BioTek产品介绍 [EB/OL ] [ 2023-03-01 ] . https://www.agilent.com/zh-cn/product/microplate-instrumentation/microplate-readers/multimode-microplate-readers https://www.agilent.com/zh-cn/product/microplate-instrumentation/microplate-readers/multimode-microplate-readers .

Product profile of Agilent BioTek [EB/OL ] [ 2023-03-01 ] . https://www.agilent.com/zh-cn/product/microplate-instrumentation/microplate-readers/multimode-microplate-readers https://www.agilent.com/zh-cn/product/microplate-instrumentation/microplate-readers/multimode-microplate-readers .

Molecular Device产品介绍 [EB/OL ] [ 2023-03-01 ] . https://www.moleculardevices.cn/microplate-readers/ https://www.moleculardevices.cn/microplate-readers/ .

Product profile of Molecular Device [EB/OL ] [ 2023-03-01 ] . https://www.moleculardevices.cn/microplate-readers/ https://www.moleculardevices.cn/microplate-readers/ .

TeCan产品介绍 [EB/OL ] [ 2023-03-01 ] . https://lifesciences.tecan.cn/microplate-readers https://lifesciences.tecan.cn/microplate-readers .

Product profile of TeCan [EB/OL ] [ 2023-03-01 ] . https://lifesciences.tecan.cn/microplate-readers https://lifesciences.tecan.cn/microplate-readers .

于学龙 , 郈秀菊 , 张素文 , 等 . 一种多通道酶标仪的研制 [J ] . 生物医学工程研究 , 2021 , 40 ( 1 ): 78 - 82 .

YU X L , HOU X J , ZHANG S W , et al . Development of a multichannel enzyme label analyzer [J ] . Journal of Biomedical Engineering Research , 2021 , 40 ( 1 ): 78 - 82 .

林学源 . 高分辨率酶标分析仪的研制 [D ] . 苏州 : 苏州大学 , 2013 .

LIN X Y . The development of high resolution microplate readers [D ] . Suzhou : Soochow University , 2013 .

朱家亮 . 基于WinCE的免疫检测酶标仪系统设计与实现 [D ] . 上海 : 上海交通大学 , 2012 .

ZHU J L . The design and implementation of the system of microplate reader by immunoassay based on WINCE [D ] . Shanghai : Shanghai Jiao Tong University , 2012 .

李强 . 全自动多功能荧光分析仪的设计与开发 [D ] . 广州 : 华南理工大学 , 2012 .

LI Q . The design and implementation of an automatic and multiple function fluorescence analyzer [D ] . Guangzhou : South China University of Technology , 2012 .

王宇 . 多功能分析仪技术研究 [D ] . 杭州 : 浙江大学 , 2016 .

WANG Y . The research of multifunctional analyzer technology [D ] . Hangzhou : Zhejiang University , 2016 .

周鹏飞 . 多功能微孔板分析仪设计与实现 [D ] . 杭州 : 杭州电子科技大学 , 2015 .

ZHOU P F . Design and implement of multi-function microplate analyzer [D ] . Hangzhou : Hangzhou Dianzi University , 2015 .

GENG X H , GAO Y , FENG C B , et al . A facile and high sensitive micro fluorimeter based on light emitting diode and photodiode [J ] . Talanta , 2017 , 175 : 183 - 188 .

DANG F , GENG X H , LI J M , et al . A miniaturized and high sensitive dual channel fluorimeter based on compact collinear optical arrangement [J ] . Talanta , 2020 , 211 : 120698 .

陈书 . 基于OPC UA的合成生物学自动化铸造平台控制系统的研究及开发 [D ] . 秦皇岛 : 燕山大学 , 2021 .

CHEN S . Research on control system for synthetic biology automated foundry based on OPC UA [D ] . Qinhuangdao : Yanshan University , 2021 .

AKASH M S H , REHMAN K . Introduction to spectrophotometric techniques [M/OL ] // Essentials of pharmaceutical analysis . Singapore: Springer Nature Singapore, 2019 : 19 - 27 [2023-03-01] . https://link.springer.com/chapter/10.1007/978-981-15-1547-7_2 https://link.springer.com/chapter/10.1007/978-981-15-1547-7_2 .

ZIMENKOV O , AMOURETTI X , GIFFORD R M , et al . Universal multidetection system for microplates : US8218141 [P ] . 2012-07-10 .

LAKOWICZ J R . Principles of fluorescence spectroscopy [M/OL ] . 3rd ed . New York : Springer , 2006 [ 2023-03-01 ] . https://link.springer.com/book/10.1007/978-0-387-46312-4 https://link.springer.com/book/10.1007/978-0-387-46312-4 .

PURNAPATRA S B , PRATIM MONDAL P . Generation of extended light-sheets for single and multi-photon fluorescence microscopy [J ] . Applied Physics Letters , 2013 , 103 ( 4 ): 043701 .

ZHAO M Y , LI B H , ZHANG H X , et al . Activatable fluorescence sensors for in vivo bio-detection in the second near-infrared window [J ] . Chemical Science , 2021 , 12 ( 10 ): 3448 - 3459 .

GOMES A J , LUNARDI C N , ROCHA F S , et al . Experimental methods in chemical engineering: fluorescence emission spectroscopy [J ] . The Canadian Journal of Chemical Engineering , 2019 , 97 ( 8 ): 2168 - 2175 .

CUCCI C , MIGNANJ A G , DALL C , et al . A portable fluorometer for the rapid screening of M1 aflatoxin [J ] . Sensors and Actuators B: Chemical , 2007 , 126 ( 2 ): 467 - 472 .

POKRZYWNICKA M , KONCKI R , TYMECKI Ł . A concept of dual optical detection using three light emitting diodes [J ] . Talanta , 2010 , 82 ( 1 ): 422 - 425 .

GENG X H , WANG N , GAO Y , et al . A novel HPLC flow cell integrated UV light emitting diode induced fluorescence detector as alternative for sensitive determination of aflatoxins [J ] . Analytica Chimica Acta , 2018 , 1033 : 81 - 86 .

陈琳 . 酶标分析仪计量检测的探讨与研究 [J ] . 计量与测试技术 , 2018 , 45 ( 11 ): 50 , 53 .

CHEN L . Discussion and research on measurement and detection of ELISA analytical instruments [J ] . Metrology & Measurement Technique , 2018 , 45 ( 11 ): 50 , 53 .

曹先海 , 庞冉冉 , 李盼盼 , 等 . 酶标仪性能验证方法建立及应用 [C ] . 第九届药物毒理学年会论文集 , 2019 : 234 - 235 .

CAO X H , PANG R R , LI P P , et al . Establishment and application of microplate reader performance verification [C ] . Proceedings of the 9th Annual Conference of Drug Toxicology , 2019 : 234 - 235 .

郭同兵 , 彭丽华 , 陈燕平 . 检定酶标仪的标准中性滤光片的校准方法及结果分析 [J ] . 中国计量 , 2020 ( 7 ): 82 - 84 .

GUO T B , PENG L H , CHEN Y P . Calibration and analysis of standard neutral filter for verification microplate reader [J ] . China Metrology , 2020 ( 7 ): 82 - 84 .

陆丰荣 , 曾玉梨 , 李国樑 , 等 . 不同酶标板与加样量对多功能酶标仪测定结果影响 [J ] . 中国职业医学 , 2019 , 46 ( 2 ): 215 - 220 .

LU F R , ZENG Y L , LI G L , et al . Effect of different types of microplate and loading volumes on the detection results of multi-function microplate reader [J ] . China Occupational Medicine , 2019 , 46 ( 2 ): 215 - 220 .

博科产品介绍 [EB/OL ] [ 2023-03-01 ] . https://www.biobase.cn/ProductCenter/Products.aspx https://www.biobase.cn/ProductCenter/Products.aspx .

Product profile of BioBase [EB/OL ] [ 2023-03-01 ] . https://www.biobase.cn/ProductCenter/Products.aspx https://www.biobase.cn/ProductCenter/Products.aspx .

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