Research progress in biosensors based on bacterial two-component systems

ZHAO Jingyu; ZHANG Jian; QI Qingsheng; WANG Qian

doi:10.12211/2096-8280.2023-016

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Research progress in biosensors based on bacterial two-component systems

Invited Review | 更新时间：2025-03-20

- Research progress in biosensors based on bacterial two-component systems
  封面论文
- Synthetic Biology Journal Vol. 5, Issue 1, Pages: 38-52(2024)
- 作者机构：
  
  山东大学，国家糖工程技术研究中心，微生物技术国家重点实验室，山东青岛 266237
- 作者简介：
- 基金信息：
- DOI：10.12211/2096-8280.2023-016
  CLC： Q81
- Received：22 February 2023，
  
  Revised：2023-07-01，
  
  Published：29 February 2024
- 稿件说明：
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赵静宇，张健，祁庆生，王倩. 基于细菌双组分系统的生物传感器的研究进展［J］. 合成生物学， 2024， 5（1）： 38-52

ZHAO Jingyu， ZHANG Jian， QI Qingsheng， WANG Qian. Research progress in biosensors based on bacterial two-component systems［J］. Synthetic Biology Journal， 2024， 5（1）： 38-52
赵静宇，张健，祁庆生，王倩. 基于细菌双组分系统的生物传感器的研究进展［J］. 合成生物学， 2024， 5（1）： 38-52 DOI： 10.12211/2096-8280.2023-016.

ZHAO Jingyu， ZHANG Jian， QI Qingsheng， WANG Qian. Research progress in biosensors based on bacterial two-component systems［J］. Synthetic Biology Journal， 2024， 5（1）： 38-52 DOI： 10.12211/2096-8280.2023-016.

摘要

细菌双组分系统能够感知和响应细胞内外的物理、化学和生物刺激，通过耦合传感和调节机制从而引起一系列的细胞反应，是一个普遍存在的信号转导通路家族。当前越来越多的合成生物学家已开始利用双组分系统的特异属性来工程化设计微生物传感系统，并应用于光遗传学、材料科学、肠道微生物组工程、生物炼制和土壤改良等领域。本综述重点介绍了开发基于双组分系统的生物传感器的最新研究进展以及在各个领域中的潜在应用。同时探讨了如何运用新的工程方法提高双组分系统传感器性能的可靠性，包括遗传重构、DNA结合结构域交换、检测阈值调节和磷酸化串扰隔离，以及如何根据特定应用的要求定制双组分系统信号特性。在未来，研究者可以将这些方法与大规模的基因合成、高通量筛选相结合，以加速和帮助发现更多未确定特征输入的双组分系统，并开发新的对广泛的刺激做出反应的基因编码生物传感器，拓展双组分生物传感器在不同领域的应用。

Abstract

Two-component systems (TCSs) in bacteria

are capable of sensing and making responses to physical

chemical

and biological stimuli within and outside the cells

and subsequently induce a wide range of cellular processes through the role played by the regulatory component and the response component in combination

which is a ubiquitous signal transduction pathway. At present

an growing number of synthetic biologists have devoted their effort to using the specific and irreplaceable properties of TCSs to design biosensors with the aim of applying in optogenetics

materials science

engineering of gut microbiome

biorefining and soil improvement

and the like. The purpose of this review is to focus on the most recent research advances in the development of biosensors based on TCSs and their potential applications. At the same time

topics of great importance are discussed on how to use novel engineering methods with synthetic biology to improve the reliability and robustness of the performance of the biosensors

such as genetic remodeling

DNA-binding domain swapping

tuning of the detection threshold and isolation of phosphorylation crosstalk as well as on how to customize the signal characteristics of TCSs to meet particular needs according to the requirements of specific applications. It would be possible in the future for scientists to combine these methods with gene synthesis on a large scale and high-throughput screening in order to speed up and give synthetic biologists a hand in the discovery of TCSs with numerous uncharacterized signal inputs and the development of genetically encoded novel biosensors that may be capable of responding to a broad range of stimuli. This allows for extending the applications of the biosensors in different fields.

关键词

Keywords

references

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