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Progress in microalgae chloroplast organelle factory development
- Synthetic Biology Journal Vol. 3, Issue 6, Pages: 1218-1234(2022)
- 作者机构：
1.中国科学院大连化学物理研究所，催化国家重点实验室，洁净能源国家实验室（筹），辽宁 大连 116023
2.大连工业大学，生物工程学院，辽宁 大连 116034
3.中国科学技术大学化学与材料科学学院，安徽 合肥 230026
- 作者简介：
- 基金信息：
DOI：10.12211/2096-8280.2022-044
CLC： Q812Received：08 August 2022，
Revised：2022-09-28，
Published：31 December 2022
- 稿件说明：
移动端阅览
朱振， 田晶， 江静， 王旺银， 曹旭鹏. 微藻叶绿体细胞器工厂研究进展［J］. 合成生物学， 2022， 3（6）： 1218-1234
ZHU Zhen， TIAN Jing， JIANG Jing， WANG Wangyin， CAO Xupeng. Progress in microalgae chloroplast organelle factory development［J］. Synthetic Biology Journal， 2022， 3（6）： 1218-1234
朱振， 田晶， 江静， 王旺银， 曹旭鹏. 微藻叶绿体细胞器工厂研究进展［J］. 合成生物学， 2022， 3（6）： 1218-1234 DOI： 10.12211/2096-8280.2022-044.
ZHU Zhen， TIAN Jing， JIANG Jing， WANG Wangyin， CAO Xupeng. Progress in microalgae chloroplast organelle factory development［J］. Synthetic Biology Journal， 2022， 3（6）： 1218-1234 DOI： 10.12211/2096-8280.2022-044.
摘要
微藻是重要的太阳能驱动CO
2
生物转化的生物，建立微藻叶绿体细胞器工厂是通过合成生物学手段实现“碳中和”的潜在途径之一。这是因为微藻叶绿体是碳同化以及后续碳水化合物、脂肪酸、天然色素、氨基酸等重要合成器官，与高等植物细胞内具备多个相对较小的叶绿体不同，大部分微藻仅拥有一个占细胞体积50%以上的大叶绿体，更有利于获得纯净的株系，有望在食品、水产、医药、化学品、生物燃料等领域占据重要地位。微藻改造及微藻叶绿体细胞器工厂研究尚处于起步阶段，本文系统通过对现有转化、表达技术进展进行汇总和简要分析，对比了叶绿体直接转化和基于叶绿体转运肽的核转化叶绿体表达不同策略的优缺点，为后续发展提供借鉴。其中，靶向叶绿体基因组的直接转化策略应用较广泛，主要集中在莱茵衣藻中，已成功表达了100多种不同的蛋白质，但是叶绿体基因组可插入位点有限和调控手段相对缺乏；通过利用叶绿体转运肽，叶绿体中90%以上的蛋白都是核编码并被可控递送到叶绿体内，因此近年来基于叶绿体转运肽的核转化叶绿体定位表达技术的关注度得到了提升，并且已经在固碳、油脂生产调节方面展示出了一定优势。
Abstract
Microalgae are important solar-driven CO
2
biotransformat
ion organisms. The chloroplasts of microalgae are important organelles for carbon assimilation and subsequent synthesis of carbohydrates
fatty acids
natural pigments
and amino acids. Unlike higher plant cells
which have multiple relatively small chloroplasts
most microalgae only have one large chloroplast that accounts for more than 50% of the cell volume. This makes it more conducive for us to obtain pure strains and it is expected to be used in food
aquatic industry
medicine
chemical products
biofuels and other fields by the establishment of microalgal chloroplast organelle factories. The construction of microalgae chloroplast organelle factories is one of the potential ways to achieve “carbon neutrality” by means of synthetic biology. The researches on microalgae transformation and microalgae chloroplast organelle factory are still in their infancy. There are still a lot of scientific and technical questions to be answered before microalgae chloroplast organelle factory can be applied at a large scale. In this mini review
the current progress of chloroplast transformation and expression technology in microalgae has been systematically summarized and briefly analyzed
and different approaches are compared
especially regarding the transformation strategies
i.e.
direct chloroplast transformation and the transformation of nuclear-encoded chloroplast-targeted genes based on chimeric chloroplast transit peptides. The direct transformation strategy targeting the chloroplast genome is widely used. In
Chlamydomonas
reinhardtii
the most studied species
more than 100 different proteins have been successfully expressed. However
the chloroplast genome has limited insertion sites and the available regulation machineries on the exogenous genes' expression are rare. By using chloroplast signal peptides
more than 90% of native chloroplast proteins are nuclear-encoded and controllably delivered to the chloroplast. In recent years
the strategy of nuclear transf
ormation and chloroplast-targeting expression based on chimeric chloroplast signal peptide has gained attention
having shown advantages in carbon fixation and oil production regulation. Some perspectives were also discussed. In the global effort for carbon neutralization
the microalgae chloroplast organelle factories will be good carriers to convert CO
2
to complex biomass by artificial and natural hybrid photosynthesis as a solution to food
energy
and environmental problems.
2
关键词
Keywords
references
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